resumo diagnostico laboratorial Micologia.pdf

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Micologia

Saúde

Resumo
Diagnóstico
laboratorial das
micoses

Diagnóstico micológico
1. Coleta do material
2. Exame direto
3. Cultura

Exame microscópico direto
• Material clínico clarificado com KOH
10-20%
• Biópsia:
-Fragmento do tecido com KOH
-Histopatológico: Coloração: Gomori,
PAS,HE

Cultura
- Técnica de complemento ao exame
microscópico
Crescimento:
- Filamentoso: 1 a 3 semanas
- Leveduriformes: 3 a 10 dias
Meio de cultura: Ágar Sabouraud
+cloranfenicol+cicloheximida

Identificação de fungos filamentosos
•Técnica do esgarçamento
•Técnica do microcultivo:
Ágar batata
•Micélio reprodutivo: Formado por
conídeos ou esporos

Identificação de leveduras
• Tubo germinativo
• Microcultivo
• Auxanograma –Assimilação de carbono
e nitrogênio
• Zimograma- Fermentação de açúcares

LAUDO EM MICOLOGIA
Material pele – Exame direto KOH

• 1a Etapa Presença de....
• 2a Etapa Forma Hifas......
Coloração Hialinas....
Septos Septadas.....
Achado característico Artroconidiadas
• 3a Etapa grupamento fúngico Sugestivo de dermatófito

#Presença de hifas hialinas septadas e artroconidiadas,
sugestivas de dermatófitos
•Laudo final: Após a cultura com o isolamento do
patôgeno
•Gênero e espécie: Microsporum canis
LAUDO FINAL

Micoses Superficiais-Pitiriase
versicolor
Exame direto

Micose Cutâneas - DERMATOFITOSE
Exame direto
cultura

Micoses Sub-cutaneas: Cromoblastomicose
Exame direto
cultura
Cladosporium Phialophora
Rhinocladiella

Micoses Sub-cutaneas: esporotricose
Exame direto de biopsia
Corado pela Prata
cultura Dimorfismo
térmico

Micoses Sub-cutaneas:micetoma
maduromicose
actinomicose
grãos

Micose Sistêmica- Paracoccidioidomicose
Exame direto
escarro
cultura
SOROLOGIA
37oC

Micose Sistêmica- Histoplasmose
Exame direto
Escovado bronquico
cultura
SOROLOGIA
37oC

Micoses oportunistas-candidiases
Exame direto
• Tubo germinativo
• Microcultivo
• Auxanograma –Assimilação de carbono e nitrogênio
• Zimograma- Fermentação de açúcares
cultura

Micoses oportunistas-
criptococose
Exame direto
Líquor
Tinta da china ou nanquim
cultura
Agar niger: criptococcus +

Micoses oportunistas-zigomicose
Exame direto ou biopsia

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resumo diagnostico laboratorial Micologia.pdf

1. Resumo Diagnóstico laboratorial das micoses

2. Diagnóstico micológico 1. Coleta do material 2. Exame direto 3. Cultura

3. Material Clínico

4. Coleta de material clínico 1o Assepsia

5. Coleta de material - Pêlo/Cabelo - Pele

6. Unha

7. -Pus -Escarro

8. Exame microscópico direto • Material clínico clarificado com KOH 10-20% • Biópsia: -Fragmento do tecido com KOH -Histopatológico: Coloração: Gomori, PAS,HE

9. Cultura - Técnica de complemento ao exame microscópico Crescimento: - Filamentoso: 1 a 3 semanas - Leveduriformes: 3 a 10 dias Meio de cultura: Ágar Sabouraud +cloranfenicol+cicloheximida

10. Identificação de fungos filamentosos •Técnica do esgarçamento •Técnica do microcultivo: Ágar batata •Micélio reprodutivo: Formado por conídeos ou esporos

11. Identificação de leveduras • Tubo germinativo • Microcultivo • Auxanograma –Assimilação de carbono e nitrogênio • Zimograma- Fermentação de açúcares

12. LAUDO EM MICOLOGIA Material pele – Exame direto KOH

13. • 1a Etapa Presença de.... • 2a Etapa Forma Hifas...... Coloração Hialinas.... Septos Septadas..... Achado característico Artroconidiadas • 3a Etapa grupamento fúngico Sugestivo de dermatófito

14. #Presença de hifas hialinas septadas e artroconidiadas, sugestivas de dermatófitos •Laudo final: Após a cultura com o isolamento do patôgeno •Gênero e espécie: Microsporum canis LAUDO FINAL

15. Micoses Superficiais-Pitiriase versicolor Exame direto

16. Micose Cutâneas - DERMATOFITOSE Exame direto cultura

17. Micoses Sub-cutaneas: Cromoblastomicose Exame direto cultura Cladosporium Phialophora Rhinocladiella

18. Micoses Sub-cutaneas: esporotricose Exame direto de biopsia Corado pela Prata cultura Dimorfismo térmico

19. Micoses Sub-cutaneas:micetoma maduromicose actinomicose grãos

20. Micose Sistêmica- Paracoccidioidomicose Exame direto escarro cultura SOROLOGIA 37oC

21. Micose Sistêmica- Histoplasmose Exame direto Escovado bronquico cultura SOROLOGIA 37oC

22. Micoses oportunistas-candidiases Exame direto • Tubo germinativo • Microcultivo • Auxanograma –Assimilação de carbono e nitrogênio • Zimograma- Fermentação de açúcares cultura

23. Micoses oportunistas- criptococose Exame direto Líquor Tinta da china ou nanquim cultura Agar niger: criptococcus +

24. Micoses oportunistas-zigomicose Exame direto ou biopsia

resumo diagnostico laboratorial Micologia.pdf

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