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1. RJ Almeida*, DSS Meireles, ACB Filho, MC Pereira, AC Lima, ACA Silva, AM Loyola, NO Dantas, CJA Sousa.
drronaldoalmeida@hotmail.com
Syntetase titanium dioxide (TiO2) nanocrystals on high degree of pureness; Evaluate its
biocompatibility following parameters defined and accepted for the Dental International
1 2
Federation ( FDI) and American Dental Association (ADA): Evidence its nanotopograph in the
interface of the new bone through Atomic Microscopy Force (AMF)
Thus, through the methodology used was possible to evidence the osseointegration phenomenal and biocompatibility of an aloplasthic material containing nanocrystals
of titanium dioxide. We can also conclude that there was direct bone deposition on the oxide, what can be called osteointegration.
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The material was gotten through the synthesis of a nano powder using the humid one way precipitation, Pechini method on ambient temperature (Fig. 1). After
temperature treatment was structurally characterized by X-ray Diffraction (XRD) (Fig. 2a), Confirming titanium dioxide synthesis on Rutilo and Anatasio phases, with
tetragonal crystalline symmetry and medium size of 21 nm (Fig. 3). We characterize using de Raman micro spectroscopy too. (Fig. 2b) The vibration asset ways typical on
TiO2 nanocrystals on Rutilo e Anatase phases, confirming the pureness on the synthesis through the absence of others nanostructures identified by Raman specter (Fig. 4).
After that, the biocompatibility test was made using intra bone implantation of the TiO2 powder inside a teflon cup in guinea pigs jaw. (Fig. 5) Previously approved by the
CEUA/UFU 101/10. Ten guinea pigs were used on the experimental periods: 4 and 12 weeks. Each animal received a Teflon cup containing TiO2 nanocrystals. From each
implant was made 24 blades with 144 cuts colored by hematoxilin-eosine method and analyzed by two independent observers by means of inflammatory intensity response
scores, considering all Teflon cup board. The analysis was criteriously interpreted following the FDI normative as acceptable or not acceptable to be related to bone.
Fig 6: (a) General vision, (b) giant cells delicate layer, (c) osteóide material, (d) Osteocite presence and TiO2 attached to bone.
Figure 7: (a) General vision, (b) appalling of the material, (c) material on gaps and bone and lamellar bone formation, (d) direct bone deposition.