1. EXPRESSION OF FOLLICLE-STIMULATING HORMONE RECEPTOR IN TUMOR BLOOD VESSELS Aurelian Radu,Ph. D, et al Laura Andrea Gómez Marín ID 000144738 Felipe Lozano Pineda ID 000172651 Molecular Biology Medicine school III semester

2. INTRODUCTION

3. FSH Is a hormone that is produced in the anterior pituitary gland. In females: FSH stimulates follicular maduration and estrogen . In males: FSH stimulates Sertolli cell proliferation spermatogenesis

4. TUMOR

5. They are anormal celular proliferation at the blood vassels cells BLOOD VESSELS TUMOR

6. http://www.google.com.co/images?hl=es&biw=1276&bih=557&gbv=2&tbs=isch%3A1&sa=1&q=BLOOD+VASSELS+TUMOR&aq=f&aqi=&aql=&oq= BLOOD VESSELS TUMOR CHARACTERISTICS

7. FSH receptor is known to be expressed only in the testicular sertolli cells and the ovarian granulosa cells. But acording to the study the FSH positive receptor expression was localizated in blood vessels surrounding the tumor (EX: breast , prostate and testis tumor) In consecuence FHS can stimulate the proliferartion and maturation of those tumor cells. RELATIONSHIP BETWEEN FSH AND TUMOR

8. GENERAL OBJECTIVE Identify the expression of follicule-stimulating Hormone receptor in tumor blood vessels Imagen de www.google.com

9. MATERIALES Y MÉTODOS

10. MUESTRAS Y ANTICUERPOS

11. FISH Y MICROSCOPIA CONFOCAL Fish cDNA antisense oligonucleotide Microscopia confocal Imagen de: www.google.com

12. INMUNOPRECIPITACIÓN, ELECTROFORESIS Y WESTERN BLOT Imagen de: www.molecular station.com

13. RESULTADOS

15. IDENTIFICACIÓN RECEPTOR DE FSH RESULTADOS

17. RESULTADOS

18. RESULTADOS Expresión del receptor en otros canceres Imagen de: www.google.com

19. RESULTADOS

20. RESULTADOS

21. DISCUSSION

22. DISCUSSION Author What he say Agree or disagree Mariani S,Ben –Josef, et al. “ two previous studies showed immunohistochemical detection of the FSH receptor in the tumor cells of prostate adenocarcinomas” Alam H, et al. “ the binding of FSH to FSH receptor in ovarian granulosa cell induces an increase in hypoxia-inducible factor 1alfa protein levels under normoxic conditions, which in turn leads to up-regulation of vascular endothelial growth factor(VEGF)”

23. DISCUSSION Author What he say Agree or disagree Fox SB, et al. “ another possibbility is suggested by the observation that in the breast tumors in humans, endothelial cells proliferate at the tumor periphery but not in the interior” Castro-Fernández C, et al. “ the fact that FSH and FSH receptor signaling is known to generate activated Gq/11 protein”

27. MAPA CONCEPTUAL FELIPE

28. http://www.molecularstation.com/es/protein/immunoprecipitation/ http://www.uco.es/grupos/inmunologiamolecular/inmunologia/tema17/etexto17.htm MARTINEZ S., Lina María. Biología molecular. 5. ed. Medellín: UPB. Fac. de Medicina, 2009. 313 p. (Notas de clase: biología molecular). LUQUE CABRERA, J y HERRAEZ SANCHEZ, A. Texto ilustrado de Biología Molecular e Ingeniería genética: conceptos, técnicas y aplicaciones en ciencias de la salud. Madrid: Elsevier Science, 2001. 469p BIBLIOGRAFÍA