Compound microscopy

UNITS OF MEASUREMENT ,[object Object],[object Object],[object Object],[object Object],[object Object]

History ,[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object]

Early Compound Microscopes ,[object Object],[object Object],[object Object]

Microscope Microbiology Cytology Histology Embryology

Simple to Complex – Life’s Levels of Organization Our journey begins here.

Properties of light ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

The light spectrum ,[object Object],Blue light 488 nm short wavelength high frequency high energy (2 times the red) Red light 650 nm long wavelength low frequency low energy Photon as a wave packet of energy

[object Object],[object Object],[object Object],[object Object]

Principles of Microscopy ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Resolution Actual What We Might See Even if we magnify an image of two objects, we can not distinguish them unless we have adequate resolution .

[object Object],[object Object],[object Object],[object Object],constant Wavelength of illumination Numerical aperture Refractive index of air or liquid between specimen & lens Sine of semi-angle of the aperture

THE MICROSCOPE ,[object Object]

THE MICROSCOPE ,[object Object],[object Object],[object Object]

How Does a Microscope Work? the eye the image the specimen Each lens magnifies the image, increasing its overall size A lens is a bi-convex disk that bends light The farther the light rays are bent, the larger the image appears The bent rays produces an image

How Does a Microscope Work? the eye the image the specimen The image is always seen upside down and backwards from its actual position

Know and be able to use the vocabulary of the microscope ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Know and be able to use the vocabulary of the microscope contd… ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Care of microscope ,[object Object],[object Object],[object Object],[object Object],[object Object]

Compound microscopy

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Compound microscopy

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