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SUTURES AND SUTURING TECHNIQUES




DISSERTATION SUBMITTED IN PARTIAL FULFILMENT OF REQUIREMENTS FOR
     THE DEGREE OF BACHELOR OF DENTAL SURGERY IN ORAL AND
                    MAXILLOFACIAL SURGERY




                    VESTA ENID LYDIA.R,
                     FINAL YEAR, B.D.S
                             2011


                               1
Dr. M.G.R MEDICAL UNIVERSITY

               C.S.I COLLEGE OF DENTAL SCIENCES AND
                              RESEARCH




                            Certificate
     This is to certify that the Library Dissertation entitled “SUTURES
AND SUTURING TECHNIQUES” was conducted by the UnderGraduate
student, VESTA ENID LYDIA.R, under my guidance and supervision in
partial fulfillment of the requirements of the Dr. M.G.R Medical University,
for the award of the degree “Bachelor of Dental Surgery”.




                                                              Dr.JayaPrakash,

                                                            Professor & H.O.D,

                                  Department of Oral & Maxillofacial Surgery

Date:
Madurai.

                                     2
ACKNOWLEDGEMENT
First of all, I would like to thank the almighty , for giving
me the strength and health to do this library dissertation
work until it be done Not forgotten to my family for
providing everything, such as money, to buy anything that
are related to this project work and their advice, which is
the most needed for this project. Internet, books, computers
and all that as my source to complete this project. They also
supported me and encouraged me to complete this task so
that I will not procrastinate in doing it.

It is with deep satisfaction and gratitude that I
acknowledgenmy guide,DR.JAYAPRAKASH ,MDS, HOD of
oral surgery CSICDSR for scholoraly guidance,help and
confidence ,encouragement which enabled me to complete
this study.

I whole heartedly thank DR.RATHNAKUMAR our
principal for providing the necessary infrastructure and
environment that is conductive for research activities,in
college.
                               3
And Im greatfull to DR.THANVIR MOHAMMED NIAZI,
MDS,
DR.ULAGANATHAN,MDS,DR.YOGANATHA,MDS, for
their mentoring heartfull discusions,continious guidance and
advices.




                             4
S.NO                    TABLE OF CONTENTS   PAGE.NO




1.     INTRODUCTION                         1


2.     REVIEW OF LITERATURE                 3


3.     ARMAMENTARIUM                        11


4.     SUTURING MATERIALS                   16

5.     ESSENTIAL SUTURE CHARACTERISTICS     30


6.     SUTURE SIZES                         35


-7.    SUTURE NEEDLES                       37


8.     PRINCIPLES OF SUTURING               47


9.     SUTURING TECHNIQUES                  49


10.    SURGICAL KNOTS                       52


11.    KNOT TYING                           61

12.    SUTURE REMOVAL                       66


13.    CONCLUSION                           67


14.    REFERENCE                            68




                                     5
6
INTRODUCTION


Wound repair is a well orchestrated and highly coordinated process that includes

a series of overlapping phases: inflammation, cell proliferation, matrix deposition,

and tissue remodeling.Sutures play an important role in wound healing after

surgical interventions and thus the selection of suture material, especially in oral

procedures, must be made carefully. This location differs from other body sites

due to the constant presence of saliva, specific microbiata, high vascularization,

as well as its functions related to speech, mastication and swallowing.


The series of pathological changes associated with several diseases ultimately

leads to severely disturbed wound healing conditions.Systemic diseases which

delay wound healing is another significant point that ef- fects the choice of suture

material and represent major clinical importanc.Diabetic wound healing

impairment is one of the most well-known chronic wound situations.The factors

ensuring appropriate intercellular communication during wound repair are not

completely understood.


The primary objective of dental suturing is to position and secure surgical flaps to

promote optimal healing. When used properly, surgical sutures should       hold flap

edges in apposition      until the wound       has healed enough to withstand normal

func- tional stresses. When the proper suture technique is used with the

appropriate thread type and diameter, tension is placed on the wound        margins
                                           7
so primary intention healing occurs.1 Accurate apposition of surgical flaps is

significant     to patient comfort, hemostasis, reduction of the wound size to be

repaired, and prevention of unnecessary bone        destruction. If surgical     wound

edges    are    not     properly approximated    and    are    therefore   inadequate,

hemostasis is present        and blood and serum may accumulate under the flap,

delaying the healing process by sep-arating the flap from the underlying bone.

Learning      how     to suture   wounds   and   lacerations   requires    a   thorough

understanding of the theory of wound care and the basic principles of suturing.we

belive that this work on sutures and suturing techniques will enlighten your

knowledge on patient care with the available source.




                                           8
REVIEW OF LITERATURE


Sometime between 50,000 and 30,000 B.C. eyed needles were invented, and by

20,000 B.C., bone needles became the standard that was not improved upon until

the Renaissance. It is reasonable to assume that these needles were used to sew

wounds together, because Neolithic (“of the ‘New’ Stone Age”) skulls have been

found, showing that trepanning (a form of surgery where a hole is drilled or

scraped into the skull) was used successfully. Evidence shows that the wounds

must have been closed up after the procedure because there is bone growth

inward from the edges of the hole;




this means that the patient was not only alive at the time of the operation, but lived

for a considerable period of time afterward.

                                          9
The primitive men in the beginning of more modern times give examples of how

early surgery was performed. Native Americans used cautery (the burning of the

body to remove or close a part of it) and East African tribes would ligate (tie off)

blood vessels with tendons and close wounds with acacia thorns pushed through

the wound with strips of leaves wound around the two protruding ends in a figure

eight.


A South African method of wound closure uses large black ants to bite the wound

edges together, with their powerful jaws acting as Michel clips. The bodies would

then be twisted off, leaving the head in the place to keep the wound closed. In

more ancient times (1,900 B.C.), the king of Babylon, Hammurabi, engraved his

country’s laws on a pillar. Some of these law related to surgical practice; one

stated that “If a physician should make a severe wound with an operating knife

and kill a patient or destroy an eye, his hands shall be cut off.”




 Because of this and similar other laws, the Babylonian practice of medicine
                                          10
declined so far that people with illness and disease were carried into the market

square so that they could get recommendations and advice from people who had

already experienced the illness.


The Mesopotamian civilizations are known to have been in regular contact with

the India and one Indian man wrote a surgical text which was a great reservoir of

information. Susruta described how to perform, in great detail, a tonsillectomy,

caesarean section, amputation, rhinoplasty and the repair of anal fistulae.




Rhinoplasty was a popular operation since the punishment for adultery was

having the offender’s nose cut off. There were many different, yet successful,

surgical procedures performed, such as the opening of the intestines and removal

of any blockage, rinsed with milk, then lubricated with butter and then finally

closed by the ant head method described before. Instruments were described in

detail in this surgical text, including triangular,round-bodied, curved, or straight

needles; sutures were made from hemp, hair, flax, and bark fiber. Training for

incisions was very important and they used melons, gourds, and animal bladders

                                         11
to practice suturing and lotus stems for ligating. It is obvious from this and other

texts that Indian surgery was considerably ahead of any other early civilization

and it can be assumed that much of Arabic, Babylonian, Egyptian, and Greek

surgery techniques originated in India.


In the seventh century B.C., the Greeks began to found medical schools because

of the great demand for surgical and medical attention; it was also at this

particular time that medicine was finally recognized as a science. A Greek

physician by the name of Hippocrates is considered to be one of the most

outstanding figures in the history of medicine.




His main contributions to surgery were his detailed clinical descriptions and the

discarding of treatments founded on tradition or wishful thinking rather than on

rationality


Sometime around 30 A.D., a medical encyclopedia was written by a Roman

named Aurelius Cornelius Celsus. His work, De Re Medicina, tells the reader that

sutures should be “soft, and not over twisted, so that they may be more easy on
                                          12
the part.” He is also credited with first substantiated mention of ligating by

recommending it as a secondary means of stopping haemmorhage


Galen, an ancient Greek physician from A.D. 150, gained a sterling reputation

from treating and suturing the severed tendons of gladiators, giving them a

chance at recovery ratherthan the sure fate of paralysis.




He was an authority on suture thread materials and has many recommendations

on which material would be best for each sort of wound closure in his book Del

Methodo Medendi. Also, Galen, along with Hippocrates, recognized two kinds of

wounds: a clean wound and a dirty wound (which required drainage before

healing could occur).


A Muslim scholar named Avicenna became known as the Prince of Physicians

because at twenty years old, he had already written extensively on philosophy,

natural history, mathematics, law, and medicine (of which he was already an

authority).


                                         13
Another development in suturing was Avicenna’s realization that some traditional

materials had a tendency to break down rapidly; because of this, he invented the

first monofilament suture by using pig’s bristles.Avicenna may have been the

Prince of Physicians, but the Prince of Surgeons was undoubtedly Albucasis. In

his first book, he recommended the indiscriminate use of cautery, but in his

second book, the use of cutting instruments and sutures were implemented

instead. In this book he described a technique called a “double suture” which is

still used today.


The technique of closing wounds by means of needle and thread is several

thousand years old. The history of surgical sutures can be traced back to ancient

Egypt, and the literature of the classical period contains a number of descriptions

of surgical techniques involving sutures.




Before catgut became the standard surgical suture material towards the end of the

19th century, many different paths had been followed to find a suitable material for
                                            14
sutures and ligatures. Materials that had been tried included gold, silver and steel

wire, silk, linen, hemp, flax, tree bark, animal and human hair, bowstrings, and gut

strings from sheep and goats.


At the beginning of the 19th century metal threads were tested as suture material.

At that time inertness of a material with respect to body tissues was considered an

advantage. Nevertheless, metal threads had major disadvantages: their stiffness

rendered knotting more difficult and could easily result in knot breakage; in

addition, suppuration of the wound edges occurred frequently.


These negative experiences with metal contributed to the establishment of silk as

the number one suture material. Wounds sewn with silk cicatrised within a few

days, and the small knot caused no problems. For these reasons most surgeons

at that time chose silk for sutures and vessel ligatures. A fundamental change in

the assessment of suture materials followed the publication in 1867 of Lister’’s

research on the prevention of wound suppuration. On the basis of work by Koch

and Pasteur, Lister concluded that wound suppuration could be prevented by

disinfecting sutures, dressings, and instruments with carbolic acid. Initially Lister

used silk as a suture material, on the assumption that it was absorbable and

therefore could also be used for ligatures. Later he searched for a more rapidly

absorbable material and consequently began to use catgut.




                                         15
Catgut is produced from animal connective tissue, in particular bovine subserosa.

Over the years it gradually emerged that animals born and bred in South America

were most suitable because they had the lowest fat content thanks to their natural

husbandry conditions.


The use of catgut was never called into question until the appearance of BSE at

the beginning of the 21st century. Alternative products had already been

developed by this time. These are the synthetically manufactured absorbable

suture materials which have largely superseded catgut in Europe. However,

catgut continues to play a major role in woundcare world-wide.


A wide variety of sterilization methods have been tested at various times.

Nowadays sutures are mostly sterilized by ethylene oxide or gamma irradiation.In

response to the requirements of modern surgery and thanks to the efforts of users

and manufacturers over the last few decades, a wide variety of sutures have now

been developed have made these sutures available to all Surgeons




                        ARMAMENTARIUM


  TOOLS FOR SUTURING


                                        16
To obtain the best results, it is important to have good quality

  instruments that are the correct size for the location and nature of the

  wounds being closed. The instruments also need to be correctly

  sterilised and handled carefully.



SURGICAL SCISSORS


Surgical scissors are classified according to the 2 blade tips - thus:


    • Sharp–sharp


    • Sharp–blunt


    • Blunt–blunt


Sometimes scissors are classified according to function – for example:


    • Suture cutting scissors


    • Dissection scissors


    In certain operations it is safer to carefully dissect your way towards an

    area/organ rather than cutting into the tissues with a sharp scalpel blade.




                                         17
                                         1
Use your medium Sharp–blunt scissors for general cutting purposes and to cut

off excess suture material after placing a suture and tying the knot.


Use the small Sharp-sharp scissors to cut the suture for removal.


SURGICAL PROBES (SEEKERS)


    • Sharp (straight)


    • Blunt (slightly curved)


Probes are also classified as:


    • Hollow


     • Solid


                                         18
A dentist uses a sharp curved probe to                                examine teeth

and   detect    cavities.Anesthetists   and                           radiologists

use flexible blunt probes to maneuver                                 their way into

specific veins or arteries in the body (for diagnostic or therapeutic purposes)




SKIN HOOK


A skin hook is used to lift a section of skin, to facilitate the placement of sutures

while minimizing the amount of injury to the tissues.By placing two skin hooks into

the tissue at the corners on the 2 sides of a laceration, and gently lifting both skin

hooks, one can facilitate eversion (having a slightly raised sutured laceration

compared to the adjacent tissue).




                                          19
                                          1
SCALPEL

A scalpel is a surgical knife with a fixed or removable blade (cutting area).

Removable blades are produced in a variety of patterns and sizes.




FORCEPS
A forceps is an instrument used in medicine to grab or to hold something.

Suture Kit contains a general-purpose tweezer-forceps. The inside of the tips

(jaws) are serrated to enhance gripping. This forceps is used for general handling

and gripping of tissue or objects.




                                        20
The other forceps is called a tissue    forceps. The tip of this forceps shows a

sharpish tip (jaws) on the one leg and a v-shaped groove on the other side. It is

commonly referred to as a rat-tooth forceps. Use this forceps to handle tissue

when placing sutures.




NEEDLE HOLDER

A Needle Holder is a special type of forceps, designed to securely hold

the surgical suture needle when placing sutures. Artery forceps are somewhat

similar in appearance, but have longer jaws – some with straight and some with

curved jaws.




                                       21
SUTURE MATERIALS




In addition to proper technique, it is critical to select the appropriate type and size

(diameter) of suture material to ensure that wound margins are free of tension,


 allowing healing by primary intention. Accurate apposition of surgical flaps

contributes to patient comfort, hemostasis, reduction of wound size, and

prevention of unnecessary bone resorption. If surgical wound edges are not

properly approximated, hemostasis can be compromised and blood/serum may


accumulate under the flap. This could result in a space between the underlying

soft tissue and bone, thus delaying the healing process. In addition, when this

occurs, healing will be by secondary intention, which can lead to irregular soft-

tissue contours and the formation of scar tissue.


Conventional intraoral surgical treatment concludes with closure of the soft tissue.

Proper suturing precisely positions the mucosal and/or mucoperiosteal flaps as

required by the surgical procedure being performed. Certain periodontal surgical
                                          22
procedures (eg, excisional new attachment procedure [ENAP] and modified

Widman flap procedure) require the surgical flap margins to be positioned in their

original location, whereas other periodontal procedures may require that the

surgical flaps be placed apically, coronally, or laterally to their original position in

order to achieve the surgical objectives.


Suturing technique, the type and diameter of suture material (thread), the type of

surgical needle, and the design of the surgical knot are essential factors in

achieving optimal wound healing. Wound closure variables are different when

suturing over hard versus soft tissue, or suturing over various types of materials

placed into the surgical site to promote periodontal regeneration (eg, bone graft

material or a membrane). The suture material and needle design will change

accordingly.


Tensile strength is an important quality when determining which suture material is

appropriate for specific situations. Tissue biocompatibility and ease of handling,

with a focus on minimal knot slippage, also influence which thread should be

selected. The clinician should select the suture material and diameter based on

the thickness of the tissue to be sutured and whether there is a need for flap

tension.


Therefore, selection of the suturing technique and material should be based on

the goals of the surgical procedure and the physical/biologic characteristics of the
                                            23
suture material in relationship to the healing process. Adequate strength of the

suture material will prevent breakage during suturing, and proper tying of the knot

in consideration of the material being used will prevent untying or knot slippage.

The clinician must also understand the nature of the suture material, the wound

healing process, the biologic forces exerted on the healing wound (eg, muscle

pulls and swelling), and the interaction of the suture and tissue. The suture must

retain its strength until the tissues of the flaps regain sufficient strength to keep the

wound edges together. In clinical situations where the tissues will not regain their

preoperative strength, or tension is exerted on the surgical flaps, consideration

should be given to using a suture material that retains long-term strength (up to 14

days) and resorbs in 21 to 28 days, such as conventional polyglycolic acid (PGA)

suture material. A clinical example would be a resorbed anterior mandible that has

muscle attachments close to the crestal ridge; when the flap margins are

reapproximated there will be tension on the margins. Should a resorbable suture

material be used that loses its tensile strength after a few days, the re-adhesion of

the periosteum to the underlying bone will not have gained enough strength to

overcome the muscle pull. Therefore, a longer-lasting suture material should be

utilized until the flap has achieved sufficient reattachment to the bone.


Resorbable sutures lose tensile strength over a period of time from several days

to several weeks, and the breakdown of the resorbable material should equal the

healing rate of the tissue being coapted by the material. If a suture is to be placed
                                           24
in tissue that heals rapidly, a resorbable suture should be used that will lose its

tensile strength at approximately the same rate as the tissue gains strength. The

suture will be absorbed by the tissue, leaving no foreign material in the wound

after healing. Examples are surgical gut or the rapidly resorbable PGA sutures

(PGA-FA).


Resorbable sutures re-sorb due to 2 mechanisms. Sutures of biological origin (eg,

surgical gut, plain and chromic gut) are gradually digested by enzymes in the

tissue, whereas resorbable sutures fabricated from synthetic materials such as

polygycolic acid are hydrolyzed via the Kreb's cycle.2 Surgical gut suture material

is made from animal protein (ie, gut), thus it can potentially induce an antigenic

reaction.6 When used intraorally, this material loses most of its tensile strength in

24 to 48 hours; coating the material with a chromic compound extends resorption

to 7 to 10 days, and extends significant tensile strength to 5 days.


An additional consideration with regard to gut su-tures is that breakage of the

material during the resorption process may occur too rapidly to maintain flap


apposition, particularly if used in patients with a very low intraoral pH.4 Many

physiological events can cause a decrease in intraoral pH, including disorders

such as epigastric reflux, hiatal hernia, and bulimia. Sjogren's syndrome,

chemotherapy, radiation therapy, and certain medications (eg, angiotensin-

converting   inhibitors,   anti-psychotics,    diuretics,   antihypertensive   agents,
                                          25
antipsoriasis medications, and steroid inhalers) can cause xerostomia and a low

intraoral pH.


Coaptation of tissue flaps requires a minimum of 5 days.5 Selection of a fast-

absorbing PGA suture is indicated in clinical situations where there is a low

intraoral pH (and surgical gut sutures are contraindicated). PGA-FA suture

material is not affected by low intraoral pH; it is manufactured from synthetic

polymers and is mainly degraded by hydrolysis in tissue fluids (via enzymes

involved in the Kreb's cycle). This requires 7 to 10 days. This material has a

higher tensile strength than surgical gut suture material, but its resorption rate is

comparable to that of surgical gut sutures under normal intraoral physiologic

conditions.


Nonresorbable sutures are fabricated either from natural or synthetic materials.

Silk has been the most widely used material for dental and many other types of

surgery. Silk is easy to handle, is tied with a slipknot, and costs less than many

other nonresorbable suture materials. However, silk sutures have certain

disadvantages. Being nonresor-bable, silk sutures must be removed by the

clinician, usually 1 week following surgery. The patient generally is not


anesthetized for this suture removal. Further, being a multifilament thread, silk

demonstrates a "wick effect," which pulls bacteria and fluids into the wound

site.9Therefore, silk is not the suture material of choice when foreign materials
                                         26
such as dental implants, bone grafts, or regenerative barriers are placed under a

mucoperiosteal flap, or when infection of the surgical site is present at the time of

surgery (ie, removal of a septic tooth).


Nonresorbable sutures that can be used in situations where silk is contraindicated

include   nylon,    polyester,    polyethylene,   polypropylene,     or   expanded

polytetrafluoroethylene (e-PTFE). Polyester sutures comprise multiple filaments of

polyester polymer, which are braided into a single strand that possesses high

tensile strength and does not weaken when moistened. A biologically inert,

nonresorbable compound of proprietary composition4 is often used to coat these

sutures to aid the suture in passing more easily through tissues. However, this

coating allows the material to untie easily unless the suture is secured with a

surgeon's knot. Nonresorbable e-PTFE suture material is a monofilament with

high tensile strength, good handling properties, and good knot security. It is,

however, expensive compared with other nonresorbable suture materials.In

addition to material composition, surgical threads are also classified by numbering

from 1 to 10; higher numbers indicate thinner, more delicate thread. 10 For

example, in implant dentistry a 3-0 thread diameter is generally used to secure

flaps when a mattress suturing technique is used, and a 4-0 thread is used closer

to the flap edges to coapt tension-free flap edges. A 4-0 thread also is used to




                                           27
secure implant surgical flaps when interrupted sutures, horizontal or vertical

mattress sutures (depending on where the tissue is positioned), and most

continuous suture techniques are utilized. In periodontal plastic surgery

procedures a 5-0 thread diameter is most often used to secure soft-tissue grafts

and transpositional/sliding pedicle flaps. When securing most other periodontal

mucoperiosteal flaps, 4-0 thread is used




 ABSORBABLE MATERIALS


Catgut plain – used to suture mucous membrane of lips, tongues superficial

laceration of the genital area. They are easily absorbed within one week.


Catgut chromic – used to suture fascia, muscles, or ligature of blood

vessels.It is usually absorbed within 30 – 45 days.


vicryl – same as above. Takes at least 70 days for absorption. Rapid vicryl is

easily absorbed.


                                           28
PDS – expensive, takes at least 5 – 6 months to be absorbed.


However, vicryl is the most commonly used suture materials during surgery

while closing in layers.


                                                                 TENSILE
                             COLOR OF                             STRENGTH ABSORPTIO
    SUTURE         TYPES              RAW MATERIAL
                             MATERIAL                             RETENTION           N RATE
                                                                  in vivo
 Surgical Gut   Plain        Yellowish- Collagen derived from    Individual       Absorbed
 Suture                      tan        healthy beef and         patient          by
                                        sheep.                   characteristics proteolytic
                             Blue Dyed                           can affect rate enzymatic
                                                                 of       tensile digestive
                                                                 strength loss. process.
 Surgical Gut   Chromic      Brown       Collagen derived from   Individual       Absorbed
 Suture                                  healthy beef and        patient          by
                             Blue Dyed   sheep.                  characteristics proteolytic
                                                                 can              enzymatic
                                                                 affect rate of digestive
                                                                 tensile          process.
                                                                 strength loss.
 (polyglactin   Braided      Violet      Copolymer of lactide    Approximately Essentially
 910) Suture                             and glycolide coated    75% remains atcomplete
                Monofilament Undyed      with polyglactin 370    two       weeks.between
                             (Natural)   and calcium stearate.   Approximately 56-70 days.
                                                                 50% remains Absorbed
                                                                 at three weeks. by
 Coated         Braided      Undyed      Copolymer of lactide    Approximately hydrolysis.
                                                                                  Essentially
 (polyglactin                (Natural)   and glycolide coated    50% remains complete by
 910)                                    with polyglactin 370    at 5 days. All 42         days.
 Suture                                  and calcium stearate.   tensile          Absorbed by
                                                                 strength is lost hydrolysis.
                                                                 at
                                                                 approximately
                                                                 14 days.




                                          29
(poliglecaprone Monofilament Undyed      Copolymer            of Approximately Complete
25) Suture                   (Natural)   glycolide and epsilon- 50-60%           at
                                         caprolactone.           (violet: 60-    91-119
                              Violet                             70%) remainsdays.
                                                                 at one week.Absorbed
                                                                 Approximately by
                                                                 20-30%          hydrolysis.
                                                                 (violet: 30-
                                                                 40%) remains
                                                                 at two weeks.
                                                                 Lost within
                                                                 three weeks
                                                                 (violet: four
                                                                 weeks).
  (polydioxanone Monofilament Violet     Polyester polymer.      Approximately Minimal until
) Suture                                                         70% remains atabout      90th
                              Blue                               two       weeks.day.
                                                                 Approximately Essentially
                              Clear                              50% remains atcomplete
                                                                 four      weeks.within six
                                                                 Approximately months.
                                                                 25% remains Absorbed
                                                                 at six weeks. by      slow
Braided         Braided                                                          hydrolysis.
                                         Copolymer of lactide Approximately Essentially
                              Undyed
Synthetic                     (White)    and glycolide coated80% remains atcomplete
Absorbable                               with       caprolactone/3        months.between 18
Suture                                   glycolide.              Approximately and
                                                                 60% remains at30 months.
                                                                 6        months.Absorbed
                                                                 Approximately by      slow
                                                                 20% remains hydrolysis.
                                                                 at 12 months.




 NON-ABSORBABLE MATERIALS


Ethilon – most commonly used to close and suture skin after surgery or

trauma to the skin. Cutting needles are usually used.


                                          30
Prolene – used to suture nerve, tendon or blood vessels.                Preferable round

body needles are used.


Silk and Linen – have similar properties. They are very strong, but they are

adherent to the tissues and can caused reaction or infection.


                                                         TENSILE
                                 COLOR OF                STRENGTH               ABSORPTIO
      SUTURE          TYPES                 RAW MATERIAL
                                 MATERIAL                RETENTION                 N RATE
                                                         in vivo
Silk               Braided       Violet   Organic        Progressive            Gradual
Suture                                    protein called degradation            encapsulatio
                                 White    fibroin.       of fiber may           n by fibrous
                                                         result       in        connective
                                                         gradual loss           tissue.
                                                         of      tensile
                                                         strength over
                                                         time.


Surgical Stainless Monofilament Silver      316L stainless steel. Indefinite.   Nonabsorbabl
Steel Suture                     metallic                                       e.
                   Multifilament

Nylon              Monofilament Violet      Long-chain         Progressive      Gradual
Suture                                      aliphatic polymers hydrolysis       encapsulatio
                                 Green      Nylon 6 or Nylon may result in      n by fibrous
                                            6,6.               gradual loss     connective
                                 Undyed
                                                               of     tensile   tissue.
                                 (Clear)
                                                               strength over
                                                               time.

Nylon              Braided       Violet     Long-chain         Progressive      Gradual
Suture                                      aliphatic polymers hydrolysis       encapsulatio
                                 Green      Nylon 6 or Nylon may result in      n by fibrous
                                            6,6.               gradual loss     connective
                                 Undyed
                                                               of     tensile   tissue.
                                 (Clear)
                                                               strength over
                                                               time.



                                            31
Polyester   Fiber Braided        Green     Poly                   No               Gradual
Suture                                     (ethylene              significant      encapsulatio
                    Monofilament Undyed    terephthalate)         change           n by fibrous
                                 (White)   .                      known to         connective
                                                                  occur in vivo.   tissue.

                    Braided      Green     Poly       (ethylene   No               Gradual
Polyester   Fiber                          terephthalate)         significant      encapsulatio
Suture                           Undyed    coated          with   change           n by fibrous
                                 (White)   polybutilate.          knownto          connective
                                                                  occur in vivo.   tissue.

Polypropylene       Monofilament Clear     Isotactic              Not subject toNonabsorbabl
Suture                                     crystalline            degradation e.
                                 Blue      stereoisomer   of      or weakening
                                           polypropylene.         by action of
                                                                  tissue
                                                                  enzymes.

              PolyMonofilament Blue        Polymer blend ofNot subject toNonabsorbabl
(hexafluoropropyle                         poly      (vinylidenedegradation e.
ne- VDF) Suture                            fluoride) and polyor weakening
                                           (vinylidene fluoride-by action of
                                           co-                  tissue
                                           hexafluoropropylene) enzymes.
                                           .



 OTHER SUTURE MATERIALS THAT ARE ALSO USED ARE:


Staples – to close wound under high tension, like scalp, trunk and extremeties.


Strips and tapes – used to close superficial laceration on the face.


Dermabond – very expensive, ideal for simple laceration, but fact around the

edges have to be removed.



                                           32
TYPES OF SUTURE MATERIALS




                        33
IDEAL SUTURE CHARACTERISTICS
                       34
The ideal suture has the following characteristics:


•   Sterile

•   All-purpose (composed of material that can be used in any surgical

    procedure)

•   Causes    minimal    tissue     injury   or    tissue   reaction   (ie,   nonelectrolytic,

    noncapillary, nonallergenic, noncarcinogenic)

•   Easy to handle

•   Holds securely when knotted (ie, no fraying or cutting)

•   High tensile strength

•   Favorable absorption profile

•   Resistant to infection


Unfortunately, at the present time, no single material can provide all of these

characteristics. In different situations and with differences in tissue composition

throughout the body, the requirements for adequate wound closure require

different suture characteristics.




    ESSENTIAL SUTURE CHARACTERISTICS

All sutures should be manufactured to assure several fundamental characteristics,

as follows:


                                              35
•   Sterility

•   Uniform diameter and size

•   Pliability for ease of handling and knot security

•   Uniform tensile strength by suture type and size


Freedom from irritants or impurities that would elicit tissue reaction


OTHER SUTURE CHARACTERISTICS


The following terms describe various characteristics related to suture material:


•   Absorbable - Progressive loss of mass and/or volume of suture material; does

    not correlate with initial tensile strength

•   Breaking strength - Limit of tensile strength at which suture failure occurs

•   Capillarity - Extent to which absorbed fluid is transferred along the suture

•   Elasticity - Measure of the ability of the material to regain its original form and

    length after deformation

•   Fluid absorption - Ability to take up fluid after immersion

•   Knot-pull tensile strength - Breaking strength of knotted suture material

    (10-40% weaker after deformation by knot placement)

•   Knot strength - Amount of force necessary to cause a knot to slip (related to

    the coefficient of static friction and plasticity of a given material)


                                             36
•   Memory - Inherent capability of suture to return to or maintain its original gross

    shape (related to elasticity, plasticity, and diameter)

•   Plasticity - Measure of the ability to deform without breaking and to maintain a

    new form after relief of the deforming force

•   Pliability - Ease of handling of suture material; ability to adjust knot tension and

    to secure knots (related to suture material, filament type, and diameter)

•   Straight-pull tensile strength - Linear breaking strength of suture material

•   Suture pullout value - The application of force to a loop of suture located where

    tissue failure occurs, which measures the strength of a particular tissue;

    variable depending on anatomic site and histologic composition (fat, 0.2 kg;

    muscle, 1.27 kg; skin, 1.82 kg; fascia, 3.77 kg)

•   Tensile strength - Measure of a material or tissue's ability to resist deformation

    and breakage


Wound breaking strength - Limit of tensile strength of a healing wound at which

separation of the wound edges occurs




                                           37
38
POSSIBLE FAILURE MECHANISMS


•   Soft tissue strength: One possible failure mechanism is suture cutting

    through the soft tissue to which it is tied. This is something all suture retaining

    devices have in common. This failure mechanism is dependent only on the

    suture, soft tissue and surgical technique so the failure mechanisms involving

    the bone anchor may be evaluated independently of the soft tissue strength.

•   Suture strength: The suture is a probable point of failure, partly because the

    suture is usually weaker than the anchor. The suture may fail at the anchor,

    knot or some unexpected flaw mechanically isolated from the anchor.

•   Bone or anchor strength:The anchor may fracture and loosen from the bone

    or the bone may fracture, resulting in anchor displacement from the bone due

    to inadequate fixation. Bone fractures are more likely to occur at bony sites

    which contain greater amounts of cancellous or more porous bone.

•   Suture fatigue resistance: Notching of the suture as the suture rubs against

    bone or the anchor during cyclic motion may result in suture breakage. This

    may not be an important issue except in special applications where healing

    would not be sufficient to bear expected loads by six weeks.

•   Anchor fatigue resistance: Cyclic stresses in the device may exceed the

    endurance limit of the anchor design, resulting in device fracture, loosening

    and loss of fixation. This may not be an important issue if the tissue heals soon

    (less than six weeks).
                                          39
SUTURE SIZES

Modern suture diameters range from thick to thin and are represented by the

series of numbers 5, 4, 3, 1, 0,2-0, 3-0, 4-0, 5-0, 6-0, 7-0, 8-0, 9-0, 10-0 and 11-0.

Number 5 sutures are heavy braided sutures used by orthopedic surgeons and

11-0 sutures are micro-fine monofilament sutures used by ophthalmic surgeons

operating with the aid of a surgical microscope. Number 5-0 or 6-0 sutures are

used to stitch up lacerations in cosmetically sensitive areas like face




                                          40
SUTURE NEEDLE

The surgical needle is composed of the point, the body, and the swaged (press-fit)

end. Classification of suture needles is usually based on their curvature, radius,

and shape. For intraoral use, three-eighths and one-half circle needles are most

commonly used.


When using the three-eighths needle to close tissue in the oral cavity, the clinician

rotates the needle on a central axis to pass it from the buccal surface to the

lingual surface in one motion, whereas the one-half circle needle is traditionally

used in more restricted areas (eg, buccal surface of maxillary molars and facial

surface of maxillary and mandibular incisors). The one-half circle needle is

routinely used for periosteal and mucogingival surgery.




Suture needles may also be classified as either conventional cutting or reverse

cutting. In the oral cavity, reverse-cutting sutures should be used to prevent the

                                         41
suture material from tearing through the papillae or edges of the surgical flap

(referred to as "cut out" ). Conventional su-ture needles are generally associated

with cut out because the inside concave (inner) curvature is sharpened; as the

needle is pulled through the tissue, it cuts the tissue. This is detrimental in dental

surgery because the tears that are created will complicate healing. In contrast, the

inner curvature of a reverse-cutting needle is smooth, with a third cutting edge

located on the convex (outer) edge.




Figure illustrates the inner curvature of a reverse-cutting needle compared to a

conventional needle. For suturing of mucoperiosteal flaps in the oral cavity, the

three-eighths reverse-cutting needle with 3-0 or 4-0 thread diameters and the one-

half reverse-cutting needle with thinner 5-0 or 6-0 thread diameters are commonly

used combinations.




                                          42
Modern needles are pre-assembled with a suitable suture material attached to the

blunt end. These needles are referred to as “atraumatic” meaning they do not

have an eye that may injure the tissue as it traverses the tissues. The needles in

your Kit have a small eye on the side opposite to the tipfor you to attach the suture

to. Atraumatic needles are manufactured in all shapes for most sizes of sutures.




ATTACHMENT OF SUTURE MATERIAL TO A NEEDLE

                                         43
In past generations, a medical professional would routinely use a needle with an

eye (an “eye” is a small hole on the blunt side of a needle where the thread is

held) for suturing purposes. The eye part of such a needle may cause minimal

damage as it traverses the tissue.

Modern suturing materials have pre-attached thread. Pre-attached sutures allow

for a smooth transition from the needle’s body to the swage and then to the suture

– and are thus referred to as an “atraumatic design” (won’t cause further injury to

the tissue).

The needle-suture attachment is an occasional weak link, and on rare occasions

may become undone. This attachment occupies about ⅛ inch (3 mm) on the

suture end of the needle (the swage). One should avoid clamping the Needle

Holder to the swage of the needle as one may interfere with the secure

attachment of the suture to the needle.

STEP 1-

Unroll about 12-16 inches (30-40 cm) of silk suture from one of the reels supplied.

STEP 2-

Remove one no 16 needle from the package using the Needle Holder. Clamp the

needle roughly in the middle of the needle’s body. Secure the Needle Holder by

clamping it to the first ratchet. (Be careful when working with sharp objects).




                                          44
STEPS I N ATTACHMENT OF

    SUTURE MATERIAL TO A NEEDLE




:




     45
STEP 3

Fold the last 1½-inch (4 cm) of suture double and pass the double thread through

the eye of the suture needle.


STEP 4

open up the double thread slightly to form a loop, and pass the needle through the

loop firmly pull the long and short loose ends of the double hread away from the

needle - thus tightening the simple loop knot to attach the thread to the needle.

STEP 5

Firmly pull the long and short loose ends of the double hread away from the

needle - thus tightening the SImple loop knot to attach the thread to the needle.

                                         4
                                         46
SUTURING TECHNIQUE GRIP

The needle holder should be held with the palm grip as illustrated in Figure 1.

This allows superior wrist mobility than if the fingers are placed in the handle

loops. The needle should be grasped between 1/3 to 1/2 of the distance between

the suture attachment and the needle tip




THE BASIC PRINCIPLES OF WOUND CARE

KNOW YOUR PATIENT

If time allows – take a good medical history, if not take a brief medical history –

but always take a medical history -Is your patient allergic to certain local

anesthetics,   antibiotics   and   pain    medication,   antiseptic   solutions   or

plasters/strapping? Does he/she suffer from chronic diseases like Diabetes or

bleeding disorders? Are they using any chronic medications? Etc

                                          47
.



GOOD VISION (GOOD LIGHTING)

Fact is that medical schools have trained a number of blind physicians over the

years – but no blind surgeon yet. Scrub sisters have a saying that the good

surgeons are those who always complain about the light – might be true, because

the whole success of the surgical procedure depends on good, proper lighting of

the operative field offering the surgeon with optimal visual sensory input!




ANESTHESIA

The surgeon will make decisions regarding local anesthesia / general anesthesia

and/or sedation. You cannot do your best for a patient who is jumping, jerking

screaming or crying all the time.

                                         48
ASEPTIC TECHNIQUE


Complete sterility of the operative field is not attainable. Sterile instruments and

suture material must be used. Excess suture material must be discarded in a

container purposed for biological waste. The needle must be discarded in a

suitable biological sharps waste container). Avoid using strong antiseptic

preparations for cleaning the wound. Most antiseptic solutions will cause damage

to the friable exposed tissue cells. In most cases a normal saline solution will be

sufficient to clean an uninfected wound!

REMOVE ALL FOREIGN MATERIAL

The removal of all foreign material must be ensured. Remove all pieces of glass,

soil, plant material etc. Soil remaining in the wound will cause a traumatic

tattooing (very difficult if not impossible to remove at a later stage!) If necessary

brush the wound with a bristled brush combined with a mild soap solution e.g.

Savlon. Leave the least number of sutures buried in the depth of the tissue - within

                                           49
the limits of getting a secure closure. Remember that suturing materials although

necessary are considered by the tissue as foreign material.

LEAVE MINIMAL DEAD SPACE

While suturing, the operator will try to suture living tissue to living tissue. Do not

leave empty spaces filled with air, blood or tissue fluid. Dead spaces produce

wonderful opportunities for bacteria to proliferate and to cause infection. Dead

space may fill up with blood clot and will contribute to the formation of excessive

scarring.




HANDLE TISSUE GENTLY

Always perform surgery - showing respect for living tissue. Careless suturing may

cause more unsightly damage compared to the original wound! Use a toothed

forceps to handle the skin (gently touch though). A flat forceps slipping all the time

will cause more damage compared to a toothed forceps handled gently.

CONTROL BLEEDING

Bleeding can be reduced with suctioning and gentle sponging, and controlled by

Electro-cautery (electrical burning) and suturing – ligate (tie-off) larger veins and

arteries and use tight suturing over bleeding areas (within reasonable limits of

course). Excessive bleeding will decrease your ability to see what you are doing

and good vision is the first principle of surgery!


                                           50
General bleeding and an inability of blood to clot may be due to a number of

medications e.g. aspirin (pain-killer), Hemophilia (a hereditary absence of clotting

factors in the blood), Liver disease, a number of blood diseases, anti-cancer

medication (chemotherapy may reduce the blood platelets which are essential for

normal blood clotting to occur) and alcohol consumption (not an infrequent finding

with patients reporting to a hospital’s emergency section). Do take a thorough

patient history before you start treating the injury!




THE REPAIR OF WOUNDS

Goals For Suturing Wounds

Optimal wound care aims at maximizing functional restoration as well as

optimizing the esthetic result. These goals must occur within the limits of

maximum patient safety and patient comfort (a calm patient experiencing

the minimal amount of pain and discomfort).

Suturing a wound may assist the healthcare professional with 3 immediate goals:

• Tight sutures will assist in controlling bleeding (securing hemostasis). It is not a

substitute for normal bleeding control measures e.g. ligating arterial bleeds in the

depth of the wound etc.

• It reduces the chances of wound infection. A closed wound is much less prone to

                                           51
wound sepsis than an open wound. Further contamination from the outside

environment is also reduced considerably!

• Reduced pain. An open wound leaves the severed sensory nerve endings open

– thus increasing pain.

Suturing a wound will optimize the traumatized tissue’s chances of retaining

its blood supply, and at the same time minimizing the formation of unsightly

scar tissue.

WOUND CLOSURE IS DIVIDED INTO:

• Primary closure – closure within the first 24 hours

• Secondary closure – wound closure more than 24 hours after the injury.

Primary closure of wounds should be the norm in most cases.

Exceptions to the rule would be highly compromised tissue where the

medical professional anticipates debridement of the wound (cleaning

and cutting away dead tissue and-or foreign material) to be necessary.

REASONS FOR WOUND BREAKDOWN

•   Suturing under tension. Suturing should be passive – do not stretch tissue and

try to close the wound under tension – it will break down!

• Sepsis. Common reasons for sutured wounds to open up again are wound

contamination by bacteria and/or foreign material.

• Poor blood supply to the wound edges due to the extent of the trauma.

• Other factors include irradiated tissue, certain systemic diseases like diabetes,

                                         52
AIDS etc.


                        PRINCIPLES OF SUTURING

  •   The needle should be grasped with the help of needle holders at

      approximately 3/4th of its distance from the tip of the needle

  • The needle should never be held at the suture end as it is the weakest point

      of the needle and grasping at this point results in either bending or

      breakage of the needle

  • The needle should pierce the tissue perpenidcular to its surface. The curved

      needles should be passed through the tissues following the curvature of the

      needle to prevent tearing of the tissue

  • The suture should be placed equidistant from the incision line

  • When one side of the incision is fixed and the other end is free, the needle

      should be passed from the free to the fixed end When one side of the tissue

      is thinner than the other side, then the needle should pass from the thinner

      to the thicker side

  •   similarly, when one side is deeper ant the other side is superficial, the

      needle should The suture should not be tied too tightly that it results in

      blanching of the tissues

  • The knot should be placed over the wound margins

  • Each suture should be placed 3-4 mm apart


                                         53
• Sometimes extra tissue might be present on one side of the incision and

      suturing it would result in ‘dog-ear’ formation

   • pass through the deeper to superficial side

   • The distance from the incision point to the needle penetration should be

      less than the depth to which the needle penetrates into the tissue




                    SUTURING TECHNIQUES

 There are many types of suture patterns available to close the incisions and

 wounds encountered daily in veterinary practice. Selecting the appropriate type

 of pattern is important to achieve not only uncomplicated wound healing, but

 also good cosmetic appearance. However, the important factors that assist in the

 selection of the appropriate pattern are not always clear. This review article

 provides some helpful hints and suggestions.

 Suture patterns are typically categorised as:

 1. continuous or interrupted

 2. inverting, appositional, or everting

 3. the effect the suture pattern has on wound tension.

The choice of using interrupted versus continuous suture patterns still remains

controversial. Perhaps the biggest advantage of continuous suture patterns is

                                           54
their speed, allowing faster wound closure, thereby          saving       anaesthetic   and

surgical time   in critically ill patients. However, interrupted patterns allow the

tension along the wound line to be more precisely controlled, adjusting tension

according to the variable spreading forces along the margin.These types of forces

are usually more of a problem with irregular wound edges. How these wound

edges look once they are apposed and the suture pattern is applied can be

descr ibed as         either inverting, appositional, or everting. For most tissue

closure, appositional suture patterns are preferable, as they allow the best

anatomical approximation        of   the disrupted tissue planes. Inverting suture

patterns have been traditionally described for the closure of hollow               viscera.

However,    studies have      shown       no added benefit of using inverting suture

patterns on routine closure of hollow viscera, and have even documented a

delay in healing when compared to appositional suture                 patterns   (Radasch

1990). An inverting pattern can sometimes be quite useful, for example to

invaginate a section of      stomach wall when managing a patient with gastric

dilatation and volvulus     whose     gastr ic    mucosal     viability    is questionable.

Otherwise, due to concerns regarding possible stricture formation and delayed

healing, inverting patterns for gastrointestinal surgery have largely fallen into

disfavour. Everting suture patterns are used primarily in areas that require

dispersal of tension forces along the wound closure line. Many of the tension

relieving suture patterns commonly in use will produce slight eversion. The

benefit   of having     slight eversion    on    skin   closure   becomes evident after
                                            55
removal of sutures (or staples), as the scar has a tendency to flatten rather than

widen. More commonly, most skin closures are accomplished using a more

traditional interrupted or continuous appositional type pattern.

ADVANTAGES AND DISADVANTAGES OF INTERRUPTED VERSUS CONTINUOUS

SUTURE PATTERNS

   INTERRUPTED SUTURE PATTERNS

   Advantages

   • Allows adjustment of tension throughout the suture line

   • Failure of one knot is often inconsequential

   Disadvantages

   • More time needed to tie individual knots

   • Poor suture economy

   Increased amount of foreign material in the wound

   CONTINUOUS SUTURE PATTERNS

   Advantages

   • Faster

   • Less foreign material in wound

   • Potentially better airtight or watertight seal

   Disadvantages

   • Failure of knot may lead to disruption of suture line

   • Less precise control of wound approximation and tension

                                          56
SURGICAL KNOTS

All suture patterns start with one basic component - the square knot. Also known

as the ‘reef ’ knot, this knot is primarily used to start and finish all suture patterns,

whether continuous or interrupted. Each square knot consists of two ‘throws’, and

by reversing directions after each throw and applying even pressure as the knot

is tightened, the resulting knot leaves the ‘tags’ of the knot coming out on the

same side of the loops. Extra throws are placed over the square knot to produce

the final knot, with the number of throws depending on the type of suture material.

As a general rule, all square knots should have a minimum of three total throws

(Rosin 1989). Extra throws beyond those necessary to produce a secure knot will

result in unnecessary extra bulk.




Failing to reverse directions while tying the knot produces a ‘granny’ knot , thus

producing ‘tags’ that exit on opposing sides of the suture loops. This knot is

inferior to the square knot because of its tendency to slip (Rosin 1989).

A surgeon’s knot , produced by passing one strand through the loop twice on the

first throw of a square knot, is occasionally used for closure of tissues where
                                           57
                                           5
tension on    the   tissues makes it difficult to apply a regular square knot.The

increase in frictional forces obtained from passing the strand through the

Surgeon’sHalf-hitch loop twice will allow a second throw to be placed without loss

of significant tightening. However, this does produce an asymmetrical knot, and

subsequent regular square knot throws must be utilised to prevent the knot from

slipping or coming undone. The increased bulk and asymmetry of the knot

makes it less suitable for general ligation than the square knot. There should

never be a need to routinely use a surgeon’s knot other than in areas where

the tension is too great to facilitate tying a square knot.




In addition, surgeon’s knots should not be utilised with catgut as the increased

friction has a tendency to make the material fray.An alternative to the surgeon’s

knot for utilisation in areas of wound tension is to tie a ‘half-hitch’ knot, slide it

down the suture line towards the pedicle, and by judiciously pulling the correct tag,

turning the half-hitch into a true square knot.This is termed a ‘sliding knot’.This

technique requires some patience and practice, but can be a very useful

addition to the       surgeon’s ar mamentar ium, particularly when            ligating

structures within deep cavities. It leaves a square knot rather than the more bulky
                                           58
                                           5
and asymmetrical surgeon’s knot. However, it must be tightened correctly in order

to avoid the suture material slipping off the pedicle.

Surgeons will often utilise a ‘buried’ knot              for subcuticular or intradermal

patterns.This knot is tied using the same knotting technique as a square knot, but

the suture is passed on the near side from deep to superficial and then across to

the far side from superficial to deep. In effect, this produces an ‘upside down’

version of the simple interrupted suture, with the knot buried in the deeper layers

of the tissues.



GENERAL PRINCIPLES OF TYING KNOTS

There are three basic methods for tying knots:

1. instrument

2. one-handed

3. two-handed tying techniques

Numerous      methods have been described for each technique, and detailed

descriptions can be found in the recommended reading list at the end of this

article. Instrument tying is the most widely used tying technique, and has the

advantage of producing consistent and reliable square knots. This technique can

be difficult to apply in deep cavities, where the one-handed tie may be more

useful.The two-handed tie produces reliably more consistent square knots than

the one-handed method, but can be slower and unwieldy in small areas. All three

techniques have their distinct advantages and disadvantages, and mastery of
                                          59
these three methods allows the surgeon to secure ligatures in a wide variety of

situations.

There are several important principles to consider when tying suture material

(Toombs and Clarke 2003):

●       Knot     secur ity   is   inversely   proportional   to diameter of the suture

material. As a general rule, use sutures no larger than 3-0 (2M) on individual

vessels and 0 (3.5M) on tissue pedicles)

●       Ensure that adequate and equal tension is applied to each strand during

knot tightening to produce a secure square knot

    •   Completed knots are left with 3 mm long tags for synthetic material and 6

        mm long tags for surgical gut. Gut must be cut long due to its tendency to

        swell and potentially loosen when exposed to tissue fluids.

●       Do     not   include frayed or damaged suture material within a knot, and

only use instrumentson the end of the suture material. This tag end will be

removed at the completion of the knot anyway.

●       Extra knots produce more bulk and potentially more tissue reaction. Only

use the recommended number of throws for your particular suture material.

EXAMPLES OF USEFUL SUTURING TECHNIQUES IN ORAL SURGERY




                                              60
A simple loop suture used Figure 6. The simple loop

                    to coapt flap margins.    suture being tied to coapt

                                      the edges of the incision.
Two suturing techniques can be used for the interrupted suture: the simple loop

and the criss-cross (which is a modification of the horizontal mattress suture

technique). In dental surgery, the simple loop is used most commonly to coapt

tension-free, mobile surgical flaps.4 Procedures where the simple loop is useful

include surgery of edentulous ridge areas, to coapt vertical releasing incisions, for

periosteal suturing, and to coapt flaps as part of certain periodontal surgical

procedures (ie, modified Wid-man flap, some periodontal regeneration surgery,

and some exploratory flap procedures). A simple loop is created by entering the

buccal flap from the epithelial surface (position 1) and crossing under the

periosteum to exit the epithelial surface of the lingual flap (position 2); a knot is

tied toward the buccal (Note: This example assumes a simple flap where all the

soft tissue has been elevated off the bone, including the periosteum.)




. A criss-cross suture placed at

an extraction site to close the

margins and aid in retention of

graft material placed in th
                                             61
socket.
The criss-cross is similar to the simple loop on the buccal aspect; however, on the

lingual aspect, the needle penetrates first through the epithelial surface of the

lingual flap, thus interposing additional suture thread between the surgical flaps.

The criss-cross technique is useful when suturing on the lingual aspect of the

man-dibular molars, especially in a patient with an active gag reflex or a large

tongue.4 A criss-cross suture is tied by entering the mesial buccal aspect (position

1) and exiting the distal buccal aspect (position 2); the suture is then crossed over

the socket, enters the mesial lingual aspect (position 3), and exits the distal lingual

aspect (position 4). The suture at the distal lingual (position 4) is tied to the free

end at the mesial buccal (position 1), and the knot is positioned toward the buccal

Interrupted sutures should be used only with tension-free mobile flaps and should

have needle penetration ap-proximately 3 mm from the wound edges or at the

base of an interdental papilla. For closing wounds with flaps free of tension, these

interrupted suture techniques achieve similar results.

The mattress technique is a variation of the interrupted suture and is usually used

in areas where tension-free flap closure cannot be accomplished.4 Mattress su-

turing techniques generally are used to resist muscle pull, to evert the wound

edges (which keeps epithelium away from underlying structures), and to adapt the

tissue flaps tightly to underlying structures when a bone graft or regenerative

membrane is used, or during dental implant surgery. A three-eighths reverse-

cutting needle with a thicker thread diameter (3-0 or 4-0) is usually used with a

                                          62
mattress suture technique1 Mattress sutures are usually left in place for 14 to 21

days before dissolution or removal.

There are variations of the mattress suture technique referred to as the horizontal

mattress and the apically or coronally repositioned vertical mattress.




                 A horizontal mattress Horizontal            mattress

               suture     showing       the suture used to closely

               sutures'    position      in adapt     nontension-free

               relation      to         the tissue around an implant

               mucogingival junction.      abutment, coupled with

                                           simple loop sutures to

                                           coapt    the   tension-free

                                           flap margins created by

                                           the horizontal    mattress

                                      suture.
When performing a mattress suture, the needle penetration through the surgical

flap should be approximately 8 mm away from the flap edge or just above the

muco-gingival junction . A horizontal mattress suture is placed by penetration of

the needle at the mesial buccal (position 1) apical to the mucogingival junction


                                          63
and is then crossed under the flap to exit at the mesial lingual (position 2). The

suture then penetrates the tissue at the distal lingual (position 3) and again

crosses under the flap to exit at the distal buccal (position 4) apical to the

mucogingival junction. The suture at the distal buccal (position 4) is tied to the free

end at the mesial buccal




               . Sling suture used to .            Lingual        view

               reposition the buccal flap demonstrating             the

               margin, independent of direction and position of

               the position of the palatal/ the suture around the

               lingual tissues. The teeth neck of the tooth.

               are utilized to help hold

              the tissue in position.
Another suture technique is the interrupted suspensory suture, commonly referred

to as the sling suture. This technique is used when only 1 side (or 1 or more

papillae of a flap) is independently repositioned to its original position or coronally

repositioned. The sling suture technique is especially useful when performing

coronally repositioned sliding flaps. When tying a sling suture, the needle enters


                                          64
the buccal flap papilla distally (position 1) and is carried lingually around the neck

of the tooth or implant to penetrate the papilla mesially (position 2), exiting

buccally. The suture is then looped back around the same tooth or implant

lingually and is tied with the free end, positioning the knot buccally. With this

technique, each suture includes the papilla on the mesial and distal aspects of

every other tooth, using separate ties.

Another variation of the interrupted suture technique is called a continuous

suture. Continuous sutures can be used to attach 2 surgical flap edges or to

secure multiple interproximal papillae of one flap independently of the other flap.

This technique offers the advantage of fewer individual suture ties; however, there

are significant disadvantages associated with this technique. If one knot or loop

breaks, the integrity of the entire surgical site will be compromised.12 For this

reason, more control can be gained using individually placed interrupted, sling,

criss-cross, or mattress sutures in lieu of placing one large continuous suture.

                            KNOT TYING

  (SQUARE KNOT)

The long end of the suture is wrapped around the tip of the closed needle holder

twice before grasping the short end of the suture with the needle holder. The first

double knot is then pulled gently tight. Two (or three) further single throws are

then added in a similar fashion to secure the knot. Each throw is pulled in the

opposite direction across the wound edge.

                                          65
SIMPLE INTERRUPTED SUTURE

The wound edge should be gently stabilised with either toothed forceps or a

skin hook. The needle should enter perpendicular to the skin 3-5mm from the

wound edge.Entering perpendicular causes a wider bite of deeper tissue to

be included in the suture than at the surface and consequently causes more

wound edge eversion and ultimately a superior cosmetic result with a thinner

scar. A common mistake is to enter the skin at a flatter angle resulting in

much less wound edge eversion The knot is then tied

.




                                      66
CONTINUOUS SUTURE



Using a continuous suture rather than multiple interrupted sutures offers a

significant time saving. However,it is not as strong as interrupted sutures,

and can strangulate the blood supply in wounds under more than minimal

tension. An interrupted suture is performed, but only the free suture end is

cut before the needle is reintroduced and directed diagonally across the

wound to exit the skin on the other side. The suture is then brought

across perpendicular to the wound edge and reintroduced on the first side

again with each bite. Once the entire wound is closed, a loop is made

with the last pass of suture, and this loop is grasp by the needle holder to

tie the knot.




VERTICAL MATTRESS SUTURE
This suture provides excellent wound support, decreases dead space,
                                     67
and provides superior wound edge eversion. The needle is introduced

  5-10mm from the wound edge and a deep bite of tissue is taken before

  exiting the skin in the same position on the opposite wound edge. The

  needle position is then reversed in the needle holder, and the needle is

  reintroduced 1-3mm from the second side of the wound and a smaller bite

  of tissue is taken before exiting on the first side of the wound. A knot can

  then be secured




HORIZONTAL MATTRESS SUTURE
This suture is especially good for distributing wound tension across larger

wounds particularly for the initial sutures. The needle is introduced 5-10mm from

the wound edge and exited on the opposite side of the wound. The needle is

then reintroduced on the second side of the wound but 3-5mm along the wound

edge from the exit point. The suture exits in the same position on the first side of

the wound and the suture is tied. The disadvantage of this suture is the risk of

strangulation of the dermal blood supply and subsequent edge necrosis.




                                         68
RUNNING SUBCUTICULAR SUTURE
 The benefit of this suture is the minimal epidermal puncture points allowing

 the suture to be left in place longer without suture-track scarring. The

 needle is introduced 10mm distal to one wound end and brought out inside

 the apex of the wound within the dermis. The free end of suture can be

 tied off on itself, or secured with a bead or crimp. Horizontal bites of dermis

 are then taken from alternating sides of the wound working towards the

 other wound apex. The second epidermal puncture is made when the

 needle exits 10mm from the other end of the wound. The second free end

 can be secured in the same way as the first. Alternatively, absorbable

 suture material can be used and the ends tied off underneath the skin

 surface.




BURIED SUTURE
                                       69
This suture is extremely important for distributing wound tension to the dermis

rather than the epidermis and also for closing dead space. It provides longer-

term support to the healing wound and improves the cosmetic result. The wound

edge is everted with a skin hook and then an absorbable suture is introduced at

the subcutaneous level and brought back out at dermal level on the same side of

the wound.. The needle then enters the same dermal level on the opposite side

of the wound and exits the in the same subcutaneous level as it was initially

entered into on the first side of the wound. The knot is tied deep at the

subcutaneous level and the free ends cut short




                                       70
SUTURE REMOVAL

The time to suture removal depends on the location and the degree of

tension the wound was closed under. This varies between surgeon and

situation, but as a general rule sutures on the head and neck are usually

removed between five and seven days post-operatively, while sutures on

trunk or extremity wounds are typically removed between ten and fourteen

days. To remove sutures, one tail of the suture should be grasp with

forceps and pulled gently towards one side to the wound, elevating the

knot. The opposite side of the suture should then be cut with stitch-cutters

or fine suture scissors immediately under the knot.. The suture can then

be pulled out of the tissue by pulling towards the opposite side of the

wound




                                     71
CONCLUSION

Suturing in essence is a surgical procedure and is governed by the basic

principles of surgery like aseptic technique etc. At the end of the day we

should be reminded that historically, surgery has been seen as a last

resort. Let us also be reminded of the famous quotation by the famous

surgeon in history, Ambrose Paré (1510–1590), who on occasion

remarked, “I dressed the wound, and God healed it!”



The body has healing mechanisms of its own. Most wounds if left for a

sufficient period of time will close completely/significantly on its own by the

process of wound contraction. Remember – do not suture each and every

single little wound – some minor cuts and bruises in esthetically unimportant

areas will heal perfectly well without suturing. Sometimes cleaning and a

small band-aid strapping is the appropriate way to manage a cut. Some

wounds may even heal better if left undisturbed by invasive measures…




                                    72
REFERENCES

 •   Surgery of the skin procedural dermatology. Ed Robinson JK, Hanke CW,

     Sengelmann RD, Siegel DM. Elsevier Mosby 2005.



 • Text book of oral and maxillofacial surgery

     Daniel.m. Laskin



 • Textbook of oral and maxillofacial surgery

     Neelima anil malik



 • Text book of oral and maxillofacial surgery

     S.M.Balaji




                                     73

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sutures and suturing techniques

  • 1. SUTURES AND SUTURING TECHNIQUES DISSERTATION SUBMITTED IN PARTIAL FULFILMENT OF REQUIREMENTS FOR THE DEGREE OF BACHELOR OF DENTAL SURGERY IN ORAL AND MAXILLOFACIAL SURGERY VESTA ENID LYDIA.R, FINAL YEAR, B.D.S 2011 1
  • 2. Dr. M.G.R MEDICAL UNIVERSITY C.S.I COLLEGE OF DENTAL SCIENCES AND RESEARCH Certificate This is to certify that the Library Dissertation entitled “SUTURES AND SUTURING TECHNIQUES” was conducted by the UnderGraduate student, VESTA ENID LYDIA.R, under my guidance and supervision in partial fulfillment of the requirements of the Dr. M.G.R Medical University, for the award of the degree “Bachelor of Dental Surgery”. Dr.JayaPrakash, Professor & H.O.D, Department of Oral & Maxillofacial Surgery Date: Madurai. 2
  • 3. ACKNOWLEDGEMENT First of all, I would like to thank the almighty , for giving me the strength and health to do this library dissertation work until it be done Not forgotten to my family for providing everything, such as money, to buy anything that are related to this project work and their advice, which is the most needed for this project. Internet, books, computers and all that as my source to complete this project. They also supported me and encouraged me to complete this task so that I will not procrastinate in doing it. It is with deep satisfaction and gratitude that I acknowledgenmy guide,DR.JAYAPRAKASH ,MDS, HOD of oral surgery CSICDSR for scholoraly guidance,help and confidence ,encouragement which enabled me to complete this study. I whole heartedly thank DR.RATHNAKUMAR our principal for providing the necessary infrastructure and environment that is conductive for research activities,in college. 3
  • 4. And Im greatfull to DR.THANVIR MOHAMMED NIAZI, MDS, DR.ULAGANATHAN,MDS,DR.YOGANATHA,MDS, for their mentoring heartfull discusions,continious guidance and advices. 4
  • 5. S.NO TABLE OF CONTENTS PAGE.NO 1. INTRODUCTION 1 2. REVIEW OF LITERATURE 3 3. ARMAMENTARIUM 11 4. SUTURING MATERIALS 16 5. ESSENTIAL SUTURE CHARACTERISTICS 30 6. SUTURE SIZES 35 -7. SUTURE NEEDLES 37 8. PRINCIPLES OF SUTURING 47 9. SUTURING TECHNIQUES 49 10. SURGICAL KNOTS 52 11. KNOT TYING 61 12. SUTURE REMOVAL 66 13. CONCLUSION 67 14. REFERENCE 68 5
  • 6. 6
  • 7. INTRODUCTION Wound repair is a well orchestrated and highly coordinated process that includes a series of overlapping phases: inflammation, cell proliferation, matrix deposition, and tissue remodeling.Sutures play an important role in wound healing after surgical interventions and thus the selection of suture material, especially in oral procedures, must be made carefully. This location differs from other body sites due to the constant presence of saliva, specific microbiata, high vascularization, as well as its functions related to speech, mastication and swallowing. The series of pathological changes associated with several diseases ultimately leads to severely disturbed wound healing conditions.Systemic diseases which delay wound healing is another significant point that ef- fects the choice of suture material and represent major clinical importanc.Diabetic wound healing impairment is one of the most well-known chronic wound situations.The factors ensuring appropriate intercellular communication during wound repair are not completely understood. The primary objective of dental suturing is to position and secure surgical flaps to promote optimal healing. When used properly, surgical sutures should hold flap edges in apposition until the wound has healed enough to withstand normal func- tional stresses. When the proper suture technique is used with the appropriate thread type and diameter, tension is placed on the wound margins 7
  • 8. so primary intention healing occurs.1 Accurate apposition of surgical flaps is significant to patient comfort, hemostasis, reduction of the wound size to be repaired, and prevention of unnecessary bone destruction. If surgical wound edges are not properly approximated and are therefore inadequate, hemostasis is present and blood and serum may accumulate under the flap, delaying the healing process by sep-arating the flap from the underlying bone. Learning how to suture wounds and lacerations requires a thorough understanding of the theory of wound care and the basic principles of suturing.we belive that this work on sutures and suturing techniques will enlighten your knowledge on patient care with the available source. 8
  • 9. REVIEW OF LITERATURE Sometime between 50,000 and 30,000 B.C. eyed needles were invented, and by 20,000 B.C., bone needles became the standard that was not improved upon until the Renaissance. It is reasonable to assume that these needles were used to sew wounds together, because Neolithic (“of the ‘New’ Stone Age”) skulls have been found, showing that trepanning (a form of surgery where a hole is drilled or scraped into the skull) was used successfully. Evidence shows that the wounds must have been closed up after the procedure because there is bone growth inward from the edges of the hole; this means that the patient was not only alive at the time of the operation, but lived for a considerable period of time afterward. 9
  • 10. The primitive men in the beginning of more modern times give examples of how early surgery was performed. Native Americans used cautery (the burning of the body to remove or close a part of it) and East African tribes would ligate (tie off) blood vessels with tendons and close wounds with acacia thorns pushed through the wound with strips of leaves wound around the two protruding ends in a figure eight. A South African method of wound closure uses large black ants to bite the wound edges together, with their powerful jaws acting as Michel clips. The bodies would then be twisted off, leaving the head in the place to keep the wound closed. In more ancient times (1,900 B.C.), the king of Babylon, Hammurabi, engraved his country’s laws on a pillar. Some of these law related to surgical practice; one stated that “If a physician should make a severe wound with an operating knife and kill a patient or destroy an eye, his hands shall be cut off.” Because of this and similar other laws, the Babylonian practice of medicine 10
  • 11. declined so far that people with illness and disease were carried into the market square so that they could get recommendations and advice from people who had already experienced the illness. The Mesopotamian civilizations are known to have been in regular contact with the India and one Indian man wrote a surgical text which was a great reservoir of information. Susruta described how to perform, in great detail, a tonsillectomy, caesarean section, amputation, rhinoplasty and the repair of anal fistulae. Rhinoplasty was a popular operation since the punishment for adultery was having the offender’s nose cut off. There were many different, yet successful, surgical procedures performed, such as the opening of the intestines and removal of any blockage, rinsed with milk, then lubricated with butter and then finally closed by the ant head method described before. Instruments were described in detail in this surgical text, including triangular,round-bodied, curved, or straight needles; sutures were made from hemp, hair, flax, and bark fiber. Training for incisions was very important and they used melons, gourds, and animal bladders 11
  • 12. to practice suturing and lotus stems for ligating. It is obvious from this and other texts that Indian surgery was considerably ahead of any other early civilization and it can be assumed that much of Arabic, Babylonian, Egyptian, and Greek surgery techniques originated in India. In the seventh century B.C., the Greeks began to found medical schools because of the great demand for surgical and medical attention; it was also at this particular time that medicine was finally recognized as a science. A Greek physician by the name of Hippocrates is considered to be one of the most outstanding figures in the history of medicine. His main contributions to surgery were his detailed clinical descriptions and the discarding of treatments founded on tradition or wishful thinking rather than on rationality Sometime around 30 A.D., a medical encyclopedia was written by a Roman named Aurelius Cornelius Celsus. His work, De Re Medicina, tells the reader that sutures should be “soft, and not over twisted, so that they may be more easy on 12
  • 13. the part.” He is also credited with first substantiated mention of ligating by recommending it as a secondary means of stopping haemmorhage Galen, an ancient Greek physician from A.D. 150, gained a sterling reputation from treating and suturing the severed tendons of gladiators, giving them a chance at recovery ratherthan the sure fate of paralysis. He was an authority on suture thread materials and has many recommendations on which material would be best for each sort of wound closure in his book Del Methodo Medendi. Also, Galen, along with Hippocrates, recognized two kinds of wounds: a clean wound and a dirty wound (which required drainage before healing could occur). A Muslim scholar named Avicenna became known as the Prince of Physicians because at twenty years old, he had already written extensively on philosophy, natural history, mathematics, law, and medicine (of which he was already an authority). 13
  • 14. Another development in suturing was Avicenna’s realization that some traditional materials had a tendency to break down rapidly; because of this, he invented the first monofilament suture by using pig’s bristles.Avicenna may have been the Prince of Physicians, but the Prince of Surgeons was undoubtedly Albucasis. In his first book, he recommended the indiscriminate use of cautery, but in his second book, the use of cutting instruments and sutures were implemented instead. In this book he described a technique called a “double suture” which is still used today. The technique of closing wounds by means of needle and thread is several thousand years old. The history of surgical sutures can be traced back to ancient Egypt, and the literature of the classical period contains a number of descriptions of surgical techniques involving sutures. Before catgut became the standard surgical suture material towards the end of the 19th century, many different paths had been followed to find a suitable material for 14
  • 15. sutures and ligatures. Materials that had been tried included gold, silver and steel wire, silk, linen, hemp, flax, tree bark, animal and human hair, bowstrings, and gut strings from sheep and goats. At the beginning of the 19th century metal threads were tested as suture material. At that time inertness of a material with respect to body tissues was considered an advantage. Nevertheless, metal threads had major disadvantages: their stiffness rendered knotting more difficult and could easily result in knot breakage; in addition, suppuration of the wound edges occurred frequently. These negative experiences with metal contributed to the establishment of silk as the number one suture material. Wounds sewn with silk cicatrised within a few days, and the small knot caused no problems. For these reasons most surgeons at that time chose silk for sutures and vessel ligatures. A fundamental change in the assessment of suture materials followed the publication in 1867 of Lister’’s research on the prevention of wound suppuration. On the basis of work by Koch and Pasteur, Lister concluded that wound suppuration could be prevented by disinfecting sutures, dressings, and instruments with carbolic acid. Initially Lister used silk as a suture material, on the assumption that it was absorbable and therefore could also be used for ligatures. Later he searched for a more rapidly absorbable material and consequently began to use catgut. 15
  • 16. Catgut is produced from animal connective tissue, in particular bovine subserosa. Over the years it gradually emerged that animals born and bred in South America were most suitable because they had the lowest fat content thanks to their natural husbandry conditions. The use of catgut was never called into question until the appearance of BSE at the beginning of the 21st century. Alternative products had already been developed by this time. These are the synthetically manufactured absorbable suture materials which have largely superseded catgut in Europe. However, catgut continues to play a major role in woundcare world-wide. A wide variety of sterilization methods have been tested at various times. Nowadays sutures are mostly sterilized by ethylene oxide or gamma irradiation.In response to the requirements of modern surgery and thanks to the efforts of users and manufacturers over the last few decades, a wide variety of sutures have now been developed have made these sutures available to all Surgeons ARMAMENTARIUM TOOLS FOR SUTURING 16
  • 17. To obtain the best results, it is important to have good quality instruments that are the correct size for the location and nature of the wounds being closed. The instruments also need to be correctly sterilised and handled carefully. SURGICAL SCISSORS Surgical scissors are classified according to the 2 blade tips - thus: • Sharp–sharp • Sharp–blunt • Blunt–blunt Sometimes scissors are classified according to function – for example: • Suture cutting scissors • Dissection scissors In certain operations it is safer to carefully dissect your way towards an area/organ rather than cutting into the tissues with a sharp scalpel blade. 17 1
  • 18. Use your medium Sharp–blunt scissors for general cutting purposes and to cut off excess suture material after placing a suture and tying the knot. Use the small Sharp-sharp scissors to cut the suture for removal. SURGICAL PROBES (SEEKERS) • Sharp (straight) • Blunt (slightly curved) Probes are also classified as: • Hollow • Solid 18
  • 19. A dentist uses a sharp curved probe to examine teeth and detect cavities.Anesthetists and radiologists use flexible blunt probes to maneuver their way into specific veins or arteries in the body (for diagnostic or therapeutic purposes) SKIN HOOK A skin hook is used to lift a section of skin, to facilitate the placement of sutures while minimizing the amount of injury to the tissues.By placing two skin hooks into the tissue at the corners on the 2 sides of a laceration, and gently lifting both skin hooks, one can facilitate eversion (having a slightly raised sutured laceration compared to the adjacent tissue). 19 1
  • 20. SCALPEL A scalpel is a surgical knife with a fixed or removable blade (cutting area). Removable blades are produced in a variety of patterns and sizes. FORCEPS A forceps is an instrument used in medicine to grab or to hold something. Suture Kit contains a general-purpose tweezer-forceps. The inside of the tips (jaws) are serrated to enhance gripping. This forceps is used for general handling and gripping of tissue or objects. 20
  • 21. The other forceps is called a tissue forceps. The tip of this forceps shows a sharpish tip (jaws) on the one leg and a v-shaped groove on the other side. It is commonly referred to as a rat-tooth forceps. Use this forceps to handle tissue when placing sutures. NEEDLE HOLDER A Needle Holder is a special type of forceps, designed to securely hold the surgical suture needle when placing sutures. Artery forceps are somewhat similar in appearance, but have longer jaws – some with straight and some with curved jaws. 21
  • 22. SUTURE MATERIALS In addition to proper technique, it is critical to select the appropriate type and size (diameter) of suture material to ensure that wound margins are free of tension, allowing healing by primary intention. Accurate apposition of surgical flaps contributes to patient comfort, hemostasis, reduction of wound size, and prevention of unnecessary bone resorption. If surgical wound edges are not properly approximated, hemostasis can be compromised and blood/serum may accumulate under the flap. This could result in a space between the underlying soft tissue and bone, thus delaying the healing process. In addition, when this occurs, healing will be by secondary intention, which can lead to irregular soft- tissue contours and the formation of scar tissue. Conventional intraoral surgical treatment concludes with closure of the soft tissue. Proper suturing precisely positions the mucosal and/or mucoperiosteal flaps as required by the surgical procedure being performed. Certain periodontal surgical 22
  • 23. procedures (eg, excisional new attachment procedure [ENAP] and modified Widman flap procedure) require the surgical flap margins to be positioned in their original location, whereas other periodontal procedures may require that the surgical flaps be placed apically, coronally, or laterally to their original position in order to achieve the surgical objectives. Suturing technique, the type and diameter of suture material (thread), the type of surgical needle, and the design of the surgical knot are essential factors in achieving optimal wound healing. Wound closure variables are different when suturing over hard versus soft tissue, or suturing over various types of materials placed into the surgical site to promote periodontal regeneration (eg, bone graft material or a membrane). The suture material and needle design will change accordingly. Tensile strength is an important quality when determining which suture material is appropriate for specific situations. Tissue biocompatibility and ease of handling, with a focus on minimal knot slippage, also influence which thread should be selected. The clinician should select the suture material and diameter based on the thickness of the tissue to be sutured and whether there is a need for flap tension. Therefore, selection of the suturing technique and material should be based on the goals of the surgical procedure and the physical/biologic characteristics of the 23
  • 24. suture material in relationship to the healing process. Adequate strength of the suture material will prevent breakage during suturing, and proper tying of the knot in consideration of the material being used will prevent untying or knot slippage. The clinician must also understand the nature of the suture material, the wound healing process, the biologic forces exerted on the healing wound (eg, muscle pulls and swelling), and the interaction of the suture and tissue. The suture must retain its strength until the tissues of the flaps regain sufficient strength to keep the wound edges together. In clinical situations where the tissues will not regain their preoperative strength, or tension is exerted on the surgical flaps, consideration should be given to using a suture material that retains long-term strength (up to 14 days) and resorbs in 21 to 28 days, such as conventional polyglycolic acid (PGA) suture material. A clinical example would be a resorbed anterior mandible that has muscle attachments close to the crestal ridge; when the flap margins are reapproximated there will be tension on the margins. Should a resorbable suture material be used that loses its tensile strength after a few days, the re-adhesion of the periosteum to the underlying bone will not have gained enough strength to overcome the muscle pull. Therefore, a longer-lasting suture material should be utilized until the flap has achieved sufficient reattachment to the bone. Resorbable sutures lose tensile strength over a period of time from several days to several weeks, and the breakdown of the resorbable material should equal the healing rate of the tissue being coapted by the material. If a suture is to be placed 24
  • 25. in tissue that heals rapidly, a resorbable suture should be used that will lose its tensile strength at approximately the same rate as the tissue gains strength. The suture will be absorbed by the tissue, leaving no foreign material in the wound after healing. Examples are surgical gut or the rapidly resorbable PGA sutures (PGA-FA). Resorbable sutures re-sorb due to 2 mechanisms. Sutures of biological origin (eg, surgical gut, plain and chromic gut) are gradually digested by enzymes in the tissue, whereas resorbable sutures fabricated from synthetic materials such as polygycolic acid are hydrolyzed via the Kreb's cycle.2 Surgical gut suture material is made from animal protein (ie, gut), thus it can potentially induce an antigenic reaction.6 When used intraorally, this material loses most of its tensile strength in 24 to 48 hours; coating the material with a chromic compound extends resorption to 7 to 10 days, and extends significant tensile strength to 5 days. An additional consideration with regard to gut su-tures is that breakage of the material during the resorption process may occur too rapidly to maintain flap apposition, particularly if used in patients with a very low intraoral pH.4 Many physiological events can cause a decrease in intraoral pH, including disorders such as epigastric reflux, hiatal hernia, and bulimia. Sjogren's syndrome, chemotherapy, radiation therapy, and certain medications (eg, angiotensin- converting inhibitors, anti-psychotics, diuretics, antihypertensive agents, 25
  • 26. antipsoriasis medications, and steroid inhalers) can cause xerostomia and a low intraoral pH. Coaptation of tissue flaps requires a minimum of 5 days.5 Selection of a fast- absorbing PGA suture is indicated in clinical situations where there is a low intraoral pH (and surgical gut sutures are contraindicated). PGA-FA suture material is not affected by low intraoral pH; it is manufactured from synthetic polymers and is mainly degraded by hydrolysis in tissue fluids (via enzymes involved in the Kreb's cycle). This requires 7 to 10 days. This material has a higher tensile strength than surgical gut suture material, but its resorption rate is comparable to that of surgical gut sutures under normal intraoral physiologic conditions. Nonresorbable sutures are fabricated either from natural or synthetic materials. Silk has been the most widely used material for dental and many other types of surgery. Silk is easy to handle, is tied with a slipknot, and costs less than many other nonresorbable suture materials. However, silk sutures have certain disadvantages. Being nonresor-bable, silk sutures must be removed by the clinician, usually 1 week following surgery. The patient generally is not anesthetized for this suture removal. Further, being a multifilament thread, silk demonstrates a "wick effect," which pulls bacteria and fluids into the wound site.9Therefore, silk is not the suture material of choice when foreign materials 26
  • 27. such as dental implants, bone grafts, or regenerative barriers are placed under a mucoperiosteal flap, or when infection of the surgical site is present at the time of surgery (ie, removal of a septic tooth). Nonresorbable sutures that can be used in situations where silk is contraindicated include nylon, polyester, polyethylene, polypropylene, or expanded polytetrafluoroethylene (e-PTFE). Polyester sutures comprise multiple filaments of polyester polymer, which are braided into a single strand that possesses high tensile strength and does not weaken when moistened. A biologically inert, nonresorbable compound of proprietary composition4 is often used to coat these sutures to aid the suture in passing more easily through tissues. However, this coating allows the material to untie easily unless the suture is secured with a surgeon's knot. Nonresorbable e-PTFE suture material is a monofilament with high tensile strength, good handling properties, and good knot security. It is, however, expensive compared with other nonresorbable suture materials.In addition to material composition, surgical threads are also classified by numbering from 1 to 10; higher numbers indicate thinner, more delicate thread. 10 For example, in implant dentistry a 3-0 thread diameter is generally used to secure flaps when a mattress suturing technique is used, and a 4-0 thread is used closer to the flap edges to coapt tension-free flap edges. A 4-0 thread also is used to 27
  • 28. secure implant surgical flaps when interrupted sutures, horizontal or vertical mattress sutures (depending on where the tissue is positioned), and most continuous suture techniques are utilized. In periodontal plastic surgery procedures a 5-0 thread diameter is most often used to secure soft-tissue grafts and transpositional/sliding pedicle flaps. When securing most other periodontal mucoperiosteal flaps, 4-0 thread is used ABSORBABLE MATERIALS Catgut plain – used to suture mucous membrane of lips, tongues superficial laceration of the genital area. They are easily absorbed within one week. Catgut chromic – used to suture fascia, muscles, or ligature of blood vessels.It is usually absorbed within 30 – 45 days. vicryl – same as above. Takes at least 70 days for absorption. Rapid vicryl is easily absorbed. 28
  • 29. PDS – expensive, takes at least 5 – 6 months to be absorbed. However, vicryl is the most commonly used suture materials during surgery while closing in layers. TENSILE COLOR OF STRENGTH ABSORPTIO SUTURE TYPES RAW MATERIAL MATERIAL RETENTION N RATE in vivo Surgical Gut Plain Yellowish- Collagen derived from Individual Absorbed Suture tan healthy beef and patient by sheep. characteristics proteolytic Blue Dyed can affect rate enzymatic of tensile digestive strength loss. process. Surgical Gut Chromic Brown Collagen derived from Individual Absorbed Suture healthy beef and patient by Blue Dyed sheep. characteristics proteolytic can enzymatic affect rate of digestive tensile process. strength loss. (polyglactin Braided Violet Copolymer of lactide Approximately Essentially 910) Suture and glycolide coated 75% remains atcomplete Monofilament Undyed with polyglactin 370 two weeks.between (Natural) and calcium stearate. Approximately 56-70 days. 50% remains Absorbed at three weeks. by Coated Braided Undyed Copolymer of lactide Approximately hydrolysis. Essentially (polyglactin (Natural) and glycolide coated 50% remains complete by 910) with polyglactin 370 at 5 days. All 42 days. Suture and calcium stearate. tensile Absorbed by strength is lost hydrolysis. at approximately 14 days. 29
  • 30. (poliglecaprone Monofilament Undyed Copolymer of Approximately Complete 25) Suture (Natural) glycolide and epsilon- 50-60% at caprolactone. (violet: 60- 91-119 Violet 70%) remainsdays. at one week.Absorbed Approximately by 20-30% hydrolysis. (violet: 30- 40%) remains at two weeks. Lost within three weeks (violet: four weeks). (polydioxanone Monofilament Violet Polyester polymer. Approximately Minimal until ) Suture 70% remains atabout 90th Blue two weeks.day. Approximately Essentially Clear 50% remains atcomplete four weeks.within six Approximately months. 25% remains Absorbed at six weeks. by slow Braided Braided hydrolysis. Copolymer of lactide Approximately Essentially Undyed Synthetic (White) and glycolide coated80% remains atcomplete Absorbable with caprolactone/3 months.between 18 Suture glycolide. Approximately and 60% remains at30 months. 6 months.Absorbed Approximately by slow 20% remains hydrolysis. at 12 months. NON-ABSORBABLE MATERIALS Ethilon – most commonly used to close and suture skin after surgery or trauma to the skin. Cutting needles are usually used. 30
  • 31. Prolene – used to suture nerve, tendon or blood vessels. Preferable round body needles are used. Silk and Linen – have similar properties. They are very strong, but they are adherent to the tissues and can caused reaction or infection. TENSILE COLOR OF STRENGTH ABSORPTIO SUTURE TYPES RAW MATERIAL MATERIAL RETENTION N RATE in vivo Silk Braided Violet Organic Progressive Gradual Suture protein called degradation encapsulatio White fibroin. of fiber may n by fibrous result in connective gradual loss tissue. of tensile strength over time. Surgical Stainless Monofilament Silver 316L stainless steel. Indefinite. Nonabsorbabl Steel Suture metallic e. Multifilament Nylon Monofilament Violet Long-chain Progressive Gradual Suture aliphatic polymers hydrolysis encapsulatio Green Nylon 6 or Nylon may result in n by fibrous 6,6. gradual loss connective Undyed of tensile tissue. (Clear) strength over time. Nylon Braided Violet Long-chain Progressive Gradual Suture aliphatic polymers hydrolysis encapsulatio Green Nylon 6 or Nylon may result in n by fibrous 6,6. gradual loss connective Undyed of tensile tissue. (Clear) strength over time. 31
  • 32. Polyester Fiber Braided Green Poly No Gradual Suture (ethylene significant encapsulatio Monofilament Undyed terephthalate) change n by fibrous (White) . known to connective occur in vivo. tissue. Braided Green Poly (ethylene No Gradual Polyester Fiber terephthalate) significant encapsulatio Suture Undyed coated with change n by fibrous (White) polybutilate. knownto connective occur in vivo. tissue. Polypropylene Monofilament Clear Isotactic Not subject toNonabsorbabl Suture crystalline degradation e. Blue stereoisomer of or weakening polypropylene. by action of tissue enzymes. PolyMonofilament Blue Polymer blend ofNot subject toNonabsorbabl (hexafluoropropyle poly (vinylidenedegradation e. ne- VDF) Suture fluoride) and polyor weakening (vinylidene fluoride-by action of co- tissue hexafluoropropylene) enzymes. . OTHER SUTURE MATERIALS THAT ARE ALSO USED ARE: Staples – to close wound under high tension, like scalp, trunk and extremeties. Strips and tapes – used to close superficial laceration on the face. Dermabond – very expensive, ideal for simple laceration, but fact around the edges have to be removed. 32
  • 33. TYPES OF SUTURE MATERIALS 33
  • 35. The ideal suture has the following characteristics: • Sterile • All-purpose (composed of material that can be used in any surgical procedure) • Causes minimal tissue injury or tissue reaction (ie, nonelectrolytic, noncapillary, nonallergenic, noncarcinogenic) • Easy to handle • Holds securely when knotted (ie, no fraying or cutting) • High tensile strength • Favorable absorption profile • Resistant to infection Unfortunately, at the present time, no single material can provide all of these characteristics. In different situations and with differences in tissue composition throughout the body, the requirements for adequate wound closure require different suture characteristics. ESSENTIAL SUTURE CHARACTERISTICS All sutures should be manufactured to assure several fundamental characteristics, as follows: 35
  • 36. Sterility • Uniform diameter and size • Pliability for ease of handling and knot security • Uniform tensile strength by suture type and size Freedom from irritants or impurities that would elicit tissue reaction OTHER SUTURE CHARACTERISTICS The following terms describe various characteristics related to suture material: • Absorbable - Progressive loss of mass and/or volume of suture material; does not correlate with initial tensile strength • Breaking strength - Limit of tensile strength at which suture failure occurs • Capillarity - Extent to which absorbed fluid is transferred along the suture • Elasticity - Measure of the ability of the material to regain its original form and length after deformation • Fluid absorption - Ability to take up fluid after immersion • Knot-pull tensile strength - Breaking strength of knotted suture material (10-40% weaker after deformation by knot placement) • Knot strength - Amount of force necessary to cause a knot to slip (related to the coefficient of static friction and plasticity of a given material) 36
  • 37. Memory - Inherent capability of suture to return to or maintain its original gross shape (related to elasticity, plasticity, and diameter) • Plasticity - Measure of the ability to deform without breaking and to maintain a new form after relief of the deforming force • Pliability - Ease of handling of suture material; ability to adjust knot tension and to secure knots (related to suture material, filament type, and diameter) • Straight-pull tensile strength - Linear breaking strength of suture material • Suture pullout value - The application of force to a loop of suture located where tissue failure occurs, which measures the strength of a particular tissue; variable depending on anatomic site and histologic composition (fat, 0.2 kg; muscle, 1.27 kg; skin, 1.82 kg; fascia, 3.77 kg) • Tensile strength - Measure of a material or tissue's ability to resist deformation and breakage Wound breaking strength - Limit of tensile strength of a healing wound at which separation of the wound edges occurs 37
  • 38. 38
  • 39. POSSIBLE FAILURE MECHANISMS • Soft tissue strength: One possible failure mechanism is suture cutting through the soft tissue to which it is tied. This is something all suture retaining devices have in common. This failure mechanism is dependent only on the suture, soft tissue and surgical technique so the failure mechanisms involving the bone anchor may be evaluated independently of the soft tissue strength. • Suture strength: The suture is a probable point of failure, partly because the suture is usually weaker than the anchor. The suture may fail at the anchor, knot or some unexpected flaw mechanically isolated from the anchor. • Bone or anchor strength:The anchor may fracture and loosen from the bone or the bone may fracture, resulting in anchor displacement from the bone due to inadequate fixation. Bone fractures are more likely to occur at bony sites which contain greater amounts of cancellous or more porous bone. • Suture fatigue resistance: Notching of the suture as the suture rubs against bone or the anchor during cyclic motion may result in suture breakage. This may not be an important issue except in special applications where healing would not be sufficient to bear expected loads by six weeks. • Anchor fatigue resistance: Cyclic stresses in the device may exceed the endurance limit of the anchor design, resulting in device fracture, loosening and loss of fixation. This may not be an important issue if the tissue heals soon (less than six weeks). 39
  • 40. SUTURE SIZES Modern suture diameters range from thick to thin and are represented by the series of numbers 5, 4, 3, 1, 0,2-0, 3-0, 4-0, 5-0, 6-0, 7-0, 8-0, 9-0, 10-0 and 11-0. Number 5 sutures are heavy braided sutures used by orthopedic surgeons and 11-0 sutures are micro-fine monofilament sutures used by ophthalmic surgeons operating with the aid of a surgical microscope. Number 5-0 or 6-0 sutures are used to stitch up lacerations in cosmetically sensitive areas like face 40
  • 41. SUTURE NEEDLE The surgical needle is composed of the point, the body, and the swaged (press-fit) end. Classification of suture needles is usually based on their curvature, radius, and shape. For intraoral use, three-eighths and one-half circle needles are most commonly used. When using the three-eighths needle to close tissue in the oral cavity, the clinician rotates the needle on a central axis to pass it from the buccal surface to the lingual surface in one motion, whereas the one-half circle needle is traditionally used in more restricted areas (eg, buccal surface of maxillary molars and facial surface of maxillary and mandibular incisors). The one-half circle needle is routinely used for periosteal and mucogingival surgery. Suture needles may also be classified as either conventional cutting or reverse cutting. In the oral cavity, reverse-cutting sutures should be used to prevent the 41
  • 42. suture material from tearing through the papillae or edges of the surgical flap (referred to as "cut out" ). Conventional su-ture needles are generally associated with cut out because the inside concave (inner) curvature is sharpened; as the needle is pulled through the tissue, it cuts the tissue. This is detrimental in dental surgery because the tears that are created will complicate healing. In contrast, the inner curvature of a reverse-cutting needle is smooth, with a third cutting edge located on the convex (outer) edge. Figure illustrates the inner curvature of a reverse-cutting needle compared to a conventional needle. For suturing of mucoperiosteal flaps in the oral cavity, the three-eighths reverse-cutting needle with 3-0 or 4-0 thread diameters and the one- half reverse-cutting needle with thinner 5-0 or 6-0 thread diameters are commonly used combinations. 42
  • 43. Modern needles are pre-assembled with a suitable suture material attached to the blunt end. These needles are referred to as “atraumatic” meaning they do not have an eye that may injure the tissue as it traverses the tissues. The needles in your Kit have a small eye on the side opposite to the tipfor you to attach the suture to. Atraumatic needles are manufactured in all shapes for most sizes of sutures. ATTACHMENT OF SUTURE MATERIAL TO A NEEDLE 43
  • 44. In past generations, a medical professional would routinely use a needle with an eye (an “eye” is a small hole on the blunt side of a needle where the thread is held) for suturing purposes. The eye part of such a needle may cause minimal damage as it traverses the tissue. Modern suturing materials have pre-attached thread. Pre-attached sutures allow for a smooth transition from the needle’s body to the swage and then to the suture – and are thus referred to as an “atraumatic design” (won’t cause further injury to the tissue). The needle-suture attachment is an occasional weak link, and on rare occasions may become undone. This attachment occupies about ⅛ inch (3 mm) on the suture end of the needle (the swage). One should avoid clamping the Needle Holder to the swage of the needle as one may interfere with the secure attachment of the suture to the needle. STEP 1- Unroll about 12-16 inches (30-40 cm) of silk suture from one of the reels supplied. STEP 2- Remove one no 16 needle from the package using the Needle Holder. Clamp the needle roughly in the middle of the needle’s body. Secure the Needle Holder by clamping it to the first ratchet. (Be careful when working with sharp objects). 44
  • 45. STEPS I N ATTACHMENT OF SUTURE MATERIAL TO A NEEDLE : 45
  • 46. STEP 3 Fold the last 1½-inch (4 cm) of suture double and pass the double thread through the eye of the suture needle. STEP 4 open up the double thread slightly to form a loop, and pass the needle through the loop firmly pull the long and short loose ends of the double hread away from the needle - thus tightening the simple loop knot to attach the thread to the needle. STEP 5 Firmly pull the long and short loose ends of the double hread away from the needle - thus tightening the SImple loop knot to attach the thread to the needle. 4 46
  • 47. SUTURING TECHNIQUE GRIP The needle holder should be held with the palm grip as illustrated in Figure 1. This allows superior wrist mobility than if the fingers are placed in the handle loops. The needle should be grasped between 1/3 to 1/2 of the distance between the suture attachment and the needle tip THE BASIC PRINCIPLES OF WOUND CARE KNOW YOUR PATIENT If time allows – take a good medical history, if not take a brief medical history – but always take a medical history -Is your patient allergic to certain local anesthetics, antibiotics and pain medication, antiseptic solutions or plasters/strapping? Does he/she suffer from chronic diseases like Diabetes or bleeding disorders? Are they using any chronic medications? Etc 47
  • 48. . GOOD VISION (GOOD LIGHTING) Fact is that medical schools have trained a number of blind physicians over the years – but no blind surgeon yet. Scrub sisters have a saying that the good surgeons are those who always complain about the light – might be true, because the whole success of the surgical procedure depends on good, proper lighting of the operative field offering the surgeon with optimal visual sensory input! ANESTHESIA The surgeon will make decisions regarding local anesthesia / general anesthesia and/or sedation. You cannot do your best for a patient who is jumping, jerking screaming or crying all the time. 48
  • 49. ASEPTIC TECHNIQUE Complete sterility of the operative field is not attainable. Sterile instruments and suture material must be used. Excess suture material must be discarded in a container purposed for biological waste. The needle must be discarded in a suitable biological sharps waste container). Avoid using strong antiseptic preparations for cleaning the wound. Most antiseptic solutions will cause damage to the friable exposed tissue cells. In most cases a normal saline solution will be sufficient to clean an uninfected wound! REMOVE ALL FOREIGN MATERIAL The removal of all foreign material must be ensured. Remove all pieces of glass, soil, plant material etc. Soil remaining in the wound will cause a traumatic tattooing (very difficult if not impossible to remove at a later stage!) If necessary brush the wound with a bristled brush combined with a mild soap solution e.g. Savlon. Leave the least number of sutures buried in the depth of the tissue - within 49
  • 50. the limits of getting a secure closure. Remember that suturing materials although necessary are considered by the tissue as foreign material. LEAVE MINIMAL DEAD SPACE While suturing, the operator will try to suture living tissue to living tissue. Do not leave empty spaces filled with air, blood or tissue fluid. Dead spaces produce wonderful opportunities for bacteria to proliferate and to cause infection. Dead space may fill up with blood clot and will contribute to the formation of excessive scarring. HANDLE TISSUE GENTLY Always perform surgery - showing respect for living tissue. Careless suturing may cause more unsightly damage compared to the original wound! Use a toothed forceps to handle the skin (gently touch though). A flat forceps slipping all the time will cause more damage compared to a toothed forceps handled gently. CONTROL BLEEDING Bleeding can be reduced with suctioning and gentle sponging, and controlled by Electro-cautery (electrical burning) and suturing – ligate (tie-off) larger veins and arteries and use tight suturing over bleeding areas (within reasonable limits of course). Excessive bleeding will decrease your ability to see what you are doing and good vision is the first principle of surgery! 50
  • 51. General bleeding and an inability of blood to clot may be due to a number of medications e.g. aspirin (pain-killer), Hemophilia (a hereditary absence of clotting factors in the blood), Liver disease, a number of blood diseases, anti-cancer medication (chemotherapy may reduce the blood platelets which are essential for normal blood clotting to occur) and alcohol consumption (not an infrequent finding with patients reporting to a hospital’s emergency section). Do take a thorough patient history before you start treating the injury! THE REPAIR OF WOUNDS Goals For Suturing Wounds Optimal wound care aims at maximizing functional restoration as well as optimizing the esthetic result. These goals must occur within the limits of maximum patient safety and patient comfort (a calm patient experiencing the minimal amount of pain and discomfort). Suturing a wound may assist the healthcare professional with 3 immediate goals: • Tight sutures will assist in controlling bleeding (securing hemostasis). It is not a substitute for normal bleeding control measures e.g. ligating arterial bleeds in the depth of the wound etc. • It reduces the chances of wound infection. A closed wound is much less prone to 51
  • 52. wound sepsis than an open wound. Further contamination from the outside environment is also reduced considerably! • Reduced pain. An open wound leaves the severed sensory nerve endings open – thus increasing pain. Suturing a wound will optimize the traumatized tissue’s chances of retaining its blood supply, and at the same time minimizing the formation of unsightly scar tissue. WOUND CLOSURE IS DIVIDED INTO: • Primary closure – closure within the first 24 hours • Secondary closure – wound closure more than 24 hours after the injury. Primary closure of wounds should be the norm in most cases. Exceptions to the rule would be highly compromised tissue where the medical professional anticipates debridement of the wound (cleaning and cutting away dead tissue and-or foreign material) to be necessary. REASONS FOR WOUND BREAKDOWN • Suturing under tension. Suturing should be passive – do not stretch tissue and try to close the wound under tension – it will break down! • Sepsis. Common reasons for sutured wounds to open up again are wound contamination by bacteria and/or foreign material. • Poor blood supply to the wound edges due to the extent of the trauma. • Other factors include irradiated tissue, certain systemic diseases like diabetes, 52
  • 53. AIDS etc. PRINCIPLES OF SUTURING • The needle should be grasped with the help of needle holders at approximately 3/4th of its distance from the tip of the needle • The needle should never be held at the suture end as it is the weakest point of the needle and grasping at this point results in either bending or breakage of the needle • The needle should pierce the tissue perpenidcular to its surface. The curved needles should be passed through the tissues following the curvature of the needle to prevent tearing of the tissue • The suture should be placed equidistant from the incision line • When one side of the incision is fixed and the other end is free, the needle should be passed from the free to the fixed end When one side of the tissue is thinner than the other side, then the needle should pass from the thinner to the thicker side • similarly, when one side is deeper ant the other side is superficial, the needle should The suture should not be tied too tightly that it results in blanching of the tissues • The knot should be placed over the wound margins • Each suture should be placed 3-4 mm apart 53
  • 54. • Sometimes extra tissue might be present on one side of the incision and suturing it would result in ‘dog-ear’ formation • pass through the deeper to superficial side • The distance from the incision point to the needle penetration should be less than the depth to which the needle penetrates into the tissue SUTURING TECHNIQUES There are many types of suture patterns available to close the incisions and wounds encountered daily in veterinary practice. Selecting the appropriate type of pattern is important to achieve not only uncomplicated wound healing, but also good cosmetic appearance. However, the important factors that assist in the selection of the appropriate pattern are not always clear. This review article provides some helpful hints and suggestions. Suture patterns are typically categorised as: 1. continuous or interrupted 2. inverting, appositional, or everting 3. the effect the suture pattern has on wound tension. The choice of using interrupted versus continuous suture patterns still remains controversial. Perhaps the biggest advantage of continuous suture patterns is 54
  • 55. their speed, allowing faster wound closure, thereby saving anaesthetic and surgical time in critically ill patients. However, interrupted patterns allow the tension along the wound line to be more precisely controlled, adjusting tension according to the variable spreading forces along the margin.These types of forces are usually more of a problem with irregular wound edges. How these wound edges look once they are apposed and the suture pattern is applied can be descr ibed as either inverting, appositional, or everting. For most tissue closure, appositional suture patterns are preferable, as they allow the best anatomical approximation of the disrupted tissue planes. Inverting suture patterns have been traditionally described for the closure of hollow viscera. However, studies have shown no added benefit of using inverting suture patterns on routine closure of hollow viscera, and have even documented a delay in healing when compared to appositional suture patterns (Radasch 1990). An inverting pattern can sometimes be quite useful, for example to invaginate a section of stomach wall when managing a patient with gastric dilatation and volvulus whose gastr ic mucosal viability is questionable. Otherwise, due to concerns regarding possible stricture formation and delayed healing, inverting patterns for gastrointestinal surgery have largely fallen into disfavour. Everting suture patterns are used primarily in areas that require dispersal of tension forces along the wound closure line. Many of the tension relieving suture patterns commonly in use will produce slight eversion. The benefit of having slight eversion on skin closure becomes evident after 55
  • 56. removal of sutures (or staples), as the scar has a tendency to flatten rather than widen. More commonly, most skin closures are accomplished using a more traditional interrupted or continuous appositional type pattern. ADVANTAGES AND DISADVANTAGES OF INTERRUPTED VERSUS CONTINUOUS SUTURE PATTERNS INTERRUPTED SUTURE PATTERNS Advantages • Allows adjustment of tension throughout the suture line • Failure of one knot is often inconsequential Disadvantages • More time needed to tie individual knots • Poor suture economy Increased amount of foreign material in the wound CONTINUOUS SUTURE PATTERNS Advantages • Faster • Less foreign material in wound • Potentially better airtight or watertight seal Disadvantages • Failure of knot may lead to disruption of suture line • Less precise control of wound approximation and tension 56
  • 57. SURGICAL KNOTS All suture patterns start with one basic component - the square knot. Also known as the ‘reef ’ knot, this knot is primarily used to start and finish all suture patterns, whether continuous or interrupted. Each square knot consists of two ‘throws’, and by reversing directions after each throw and applying even pressure as the knot is tightened, the resulting knot leaves the ‘tags’ of the knot coming out on the same side of the loops. Extra throws are placed over the square knot to produce the final knot, with the number of throws depending on the type of suture material. As a general rule, all square knots should have a minimum of three total throws (Rosin 1989). Extra throws beyond those necessary to produce a secure knot will result in unnecessary extra bulk. Failing to reverse directions while tying the knot produces a ‘granny’ knot , thus producing ‘tags’ that exit on opposing sides of the suture loops. This knot is inferior to the square knot because of its tendency to slip (Rosin 1989). A surgeon’s knot , produced by passing one strand through the loop twice on the first throw of a square knot, is occasionally used for closure of tissues where 57 5
  • 58. tension on the tissues makes it difficult to apply a regular square knot.The increase in frictional forces obtained from passing the strand through the Surgeon’sHalf-hitch loop twice will allow a second throw to be placed without loss of significant tightening. However, this does produce an asymmetrical knot, and subsequent regular square knot throws must be utilised to prevent the knot from slipping or coming undone. The increased bulk and asymmetry of the knot makes it less suitable for general ligation than the square knot. There should never be a need to routinely use a surgeon’s knot other than in areas where the tension is too great to facilitate tying a square knot. In addition, surgeon’s knots should not be utilised with catgut as the increased friction has a tendency to make the material fray.An alternative to the surgeon’s knot for utilisation in areas of wound tension is to tie a ‘half-hitch’ knot, slide it down the suture line towards the pedicle, and by judiciously pulling the correct tag, turning the half-hitch into a true square knot.This is termed a ‘sliding knot’.This technique requires some patience and practice, but can be a very useful addition to the surgeon’s ar mamentar ium, particularly when ligating structures within deep cavities. It leaves a square knot rather than the more bulky 58 5
  • 59. and asymmetrical surgeon’s knot. However, it must be tightened correctly in order to avoid the suture material slipping off the pedicle. Surgeons will often utilise a ‘buried’ knot for subcuticular or intradermal patterns.This knot is tied using the same knotting technique as a square knot, but the suture is passed on the near side from deep to superficial and then across to the far side from superficial to deep. In effect, this produces an ‘upside down’ version of the simple interrupted suture, with the knot buried in the deeper layers of the tissues. GENERAL PRINCIPLES OF TYING KNOTS There are three basic methods for tying knots: 1. instrument 2. one-handed 3. two-handed tying techniques Numerous methods have been described for each technique, and detailed descriptions can be found in the recommended reading list at the end of this article. Instrument tying is the most widely used tying technique, and has the advantage of producing consistent and reliable square knots. This technique can be difficult to apply in deep cavities, where the one-handed tie may be more useful.The two-handed tie produces reliably more consistent square knots than the one-handed method, but can be slower and unwieldy in small areas. All three techniques have their distinct advantages and disadvantages, and mastery of 59
  • 60. these three methods allows the surgeon to secure ligatures in a wide variety of situations. There are several important principles to consider when tying suture material (Toombs and Clarke 2003): ● Knot secur ity is inversely proportional to diameter of the suture material. As a general rule, use sutures no larger than 3-0 (2M) on individual vessels and 0 (3.5M) on tissue pedicles) ● Ensure that adequate and equal tension is applied to each strand during knot tightening to produce a secure square knot • Completed knots are left with 3 mm long tags for synthetic material and 6 mm long tags for surgical gut. Gut must be cut long due to its tendency to swell and potentially loosen when exposed to tissue fluids. ● Do not include frayed or damaged suture material within a knot, and only use instrumentson the end of the suture material. This tag end will be removed at the completion of the knot anyway. ● Extra knots produce more bulk and potentially more tissue reaction. Only use the recommended number of throws for your particular suture material. EXAMPLES OF USEFUL SUTURING TECHNIQUES IN ORAL SURGERY 60
  • 61. A simple loop suture used Figure 6. The simple loop to coapt flap margins. suture being tied to coapt the edges of the incision. Two suturing techniques can be used for the interrupted suture: the simple loop and the criss-cross (which is a modification of the horizontal mattress suture technique). In dental surgery, the simple loop is used most commonly to coapt tension-free, mobile surgical flaps.4 Procedures where the simple loop is useful include surgery of edentulous ridge areas, to coapt vertical releasing incisions, for periosteal suturing, and to coapt flaps as part of certain periodontal surgical procedures (ie, modified Wid-man flap, some periodontal regeneration surgery, and some exploratory flap procedures). A simple loop is created by entering the buccal flap from the epithelial surface (position 1) and crossing under the periosteum to exit the epithelial surface of the lingual flap (position 2); a knot is tied toward the buccal (Note: This example assumes a simple flap where all the soft tissue has been elevated off the bone, including the periosteum.) . A criss-cross suture placed at an extraction site to close the margins and aid in retention of graft material placed in th 61
  • 62. socket. The criss-cross is similar to the simple loop on the buccal aspect; however, on the lingual aspect, the needle penetrates first through the epithelial surface of the lingual flap, thus interposing additional suture thread between the surgical flaps. The criss-cross technique is useful when suturing on the lingual aspect of the man-dibular molars, especially in a patient with an active gag reflex or a large tongue.4 A criss-cross suture is tied by entering the mesial buccal aspect (position 1) and exiting the distal buccal aspect (position 2); the suture is then crossed over the socket, enters the mesial lingual aspect (position 3), and exits the distal lingual aspect (position 4). The suture at the distal lingual (position 4) is tied to the free end at the mesial buccal (position 1), and the knot is positioned toward the buccal Interrupted sutures should be used only with tension-free mobile flaps and should have needle penetration ap-proximately 3 mm from the wound edges or at the base of an interdental papilla. For closing wounds with flaps free of tension, these interrupted suture techniques achieve similar results. The mattress technique is a variation of the interrupted suture and is usually used in areas where tension-free flap closure cannot be accomplished.4 Mattress su- turing techniques generally are used to resist muscle pull, to evert the wound edges (which keeps epithelium away from underlying structures), and to adapt the tissue flaps tightly to underlying structures when a bone graft or regenerative membrane is used, or during dental implant surgery. A three-eighths reverse- cutting needle with a thicker thread diameter (3-0 or 4-0) is usually used with a 62
  • 63. mattress suture technique1 Mattress sutures are usually left in place for 14 to 21 days before dissolution or removal. There are variations of the mattress suture technique referred to as the horizontal mattress and the apically or coronally repositioned vertical mattress. A horizontal mattress Horizontal mattress suture showing the suture used to closely sutures' position in adapt nontension-free relation to the tissue around an implant mucogingival junction. abutment, coupled with simple loop sutures to coapt the tension-free flap margins created by the horizontal mattress suture. When performing a mattress suture, the needle penetration through the surgical flap should be approximately 8 mm away from the flap edge or just above the muco-gingival junction . A horizontal mattress suture is placed by penetration of the needle at the mesial buccal (position 1) apical to the mucogingival junction 63
  • 64. and is then crossed under the flap to exit at the mesial lingual (position 2). The suture then penetrates the tissue at the distal lingual (position 3) and again crosses under the flap to exit at the distal buccal (position 4) apical to the mucogingival junction. The suture at the distal buccal (position 4) is tied to the free end at the mesial buccal . Sling suture used to . Lingual view reposition the buccal flap demonstrating the margin, independent of direction and position of the position of the palatal/ the suture around the lingual tissues. The teeth neck of the tooth. are utilized to help hold the tissue in position. Another suture technique is the interrupted suspensory suture, commonly referred to as the sling suture. This technique is used when only 1 side (or 1 or more papillae of a flap) is independently repositioned to its original position or coronally repositioned. The sling suture technique is especially useful when performing coronally repositioned sliding flaps. When tying a sling suture, the needle enters 64
  • 65. the buccal flap papilla distally (position 1) and is carried lingually around the neck of the tooth or implant to penetrate the papilla mesially (position 2), exiting buccally. The suture is then looped back around the same tooth or implant lingually and is tied with the free end, positioning the knot buccally. With this technique, each suture includes the papilla on the mesial and distal aspects of every other tooth, using separate ties. Another variation of the interrupted suture technique is called a continuous suture. Continuous sutures can be used to attach 2 surgical flap edges or to secure multiple interproximal papillae of one flap independently of the other flap. This technique offers the advantage of fewer individual suture ties; however, there are significant disadvantages associated with this technique. If one knot or loop breaks, the integrity of the entire surgical site will be compromised.12 For this reason, more control can be gained using individually placed interrupted, sling, criss-cross, or mattress sutures in lieu of placing one large continuous suture. KNOT TYING (SQUARE KNOT) The long end of the suture is wrapped around the tip of the closed needle holder twice before grasping the short end of the suture with the needle holder. The first double knot is then pulled gently tight. Two (or three) further single throws are then added in a similar fashion to secure the knot. Each throw is pulled in the opposite direction across the wound edge. 65
  • 66. SIMPLE INTERRUPTED SUTURE The wound edge should be gently stabilised with either toothed forceps or a skin hook. The needle should enter perpendicular to the skin 3-5mm from the wound edge.Entering perpendicular causes a wider bite of deeper tissue to be included in the suture than at the surface and consequently causes more wound edge eversion and ultimately a superior cosmetic result with a thinner scar. A common mistake is to enter the skin at a flatter angle resulting in much less wound edge eversion The knot is then tied . 66
  • 67. CONTINUOUS SUTURE Using a continuous suture rather than multiple interrupted sutures offers a significant time saving. However,it is not as strong as interrupted sutures, and can strangulate the blood supply in wounds under more than minimal tension. An interrupted suture is performed, but only the free suture end is cut before the needle is reintroduced and directed diagonally across the wound to exit the skin on the other side. The suture is then brought across perpendicular to the wound edge and reintroduced on the first side again with each bite. Once the entire wound is closed, a loop is made with the last pass of suture, and this loop is grasp by the needle holder to tie the knot. VERTICAL MATTRESS SUTURE This suture provides excellent wound support, decreases dead space, 67
  • 68. and provides superior wound edge eversion. The needle is introduced 5-10mm from the wound edge and a deep bite of tissue is taken before exiting the skin in the same position on the opposite wound edge. The needle position is then reversed in the needle holder, and the needle is reintroduced 1-3mm from the second side of the wound and a smaller bite of tissue is taken before exiting on the first side of the wound. A knot can then be secured HORIZONTAL MATTRESS SUTURE This suture is especially good for distributing wound tension across larger wounds particularly for the initial sutures. The needle is introduced 5-10mm from the wound edge and exited on the opposite side of the wound. The needle is then reintroduced on the second side of the wound but 3-5mm along the wound edge from the exit point. The suture exits in the same position on the first side of the wound and the suture is tied. The disadvantage of this suture is the risk of strangulation of the dermal blood supply and subsequent edge necrosis. 68
  • 69. RUNNING SUBCUTICULAR SUTURE The benefit of this suture is the minimal epidermal puncture points allowing the suture to be left in place longer without suture-track scarring. The needle is introduced 10mm distal to one wound end and brought out inside the apex of the wound within the dermis. The free end of suture can be tied off on itself, or secured with a bead or crimp. Horizontal bites of dermis are then taken from alternating sides of the wound working towards the other wound apex. The second epidermal puncture is made when the needle exits 10mm from the other end of the wound. The second free end can be secured in the same way as the first. Alternatively, absorbable suture material can be used and the ends tied off underneath the skin surface. BURIED SUTURE 69
  • 70. This suture is extremely important for distributing wound tension to the dermis rather than the epidermis and also for closing dead space. It provides longer- term support to the healing wound and improves the cosmetic result. The wound edge is everted with a skin hook and then an absorbable suture is introduced at the subcutaneous level and brought back out at dermal level on the same side of the wound.. The needle then enters the same dermal level on the opposite side of the wound and exits the in the same subcutaneous level as it was initially entered into on the first side of the wound. The knot is tied deep at the subcutaneous level and the free ends cut short 70
  • 71. SUTURE REMOVAL The time to suture removal depends on the location and the degree of tension the wound was closed under. This varies between surgeon and situation, but as a general rule sutures on the head and neck are usually removed between five and seven days post-operatively, while sutures on trunk or extremity wounds are typically removed between ten and fourteen days. To remove sutures, one tail of the suture should be grasp with forceps and pulled gently towards one side to the wound, elevating the knot. The opposite side of the suture should then be cut with stitch-cutters or fine suture scissors immediately under the knot.. The suture can then be pulled out of the tissue by pulling towards the opposite side of the wound 71
  • 72. CONCLUSION Suturing in essence is a surgical procedure and is governed by the basic principles of surgery like aseptic technique etc. At the end of the day we should be reminded that historically, surgery has been seen as a last resort. Let us also be reminded of the famous quotation by the famous surgeon in history, Ambrose Paré (1510–1590), who on occasion remarked, “I dressed the wound, and God healed it!” The body has healing mechanisms of its own. Most wounds if left for a sufficient period of time will close completely/significantly on its own by the process of wound contraction. Remember – do not suture each and every single little wound – some minor cuts and bruises in esthetically unimportant areas will heal perfectly well without suturing. Sometimes cleaning and a small band-aid strapping is the appropriate way to manage a cut. Some wounds may even heal better if left undisturbed by invasive measures… 72
  • 73. REFERENCES • Surgery of the skin procedural dermatology. Ed Robinson JK, Hanke CW, Sengelmann RD, Siegel DM. Elsevier Mosby 2005. • Text book of oral and maxillofacial surgery Daniel.m. Laskin • Textbook of oral and maxillofacial surgery Neelima anil malik • Text book of oral and maxillofacial surgery S.M.Balaji 73