Rapid UHPLC Determination of Common Preservatives in Cosmetic Products v2zq
This document presents a fast UHPLC method for quantifying six common parabens (preservatives) in cosmetic products. The UHPLC method using a 1.9 μm column achieved a run time of 3.5 minutes, an over 80% reduction compared to the 19 minute run time using a conventional HPLC column. The UHPLC method also reduced solvent usage by over 90%. The method was shown to be linear, precise, and able to detect parabens at levels within EU and FDA regulations using samples of face lotion and body lotion.
This document discusses honey adulteration, including direct adulteration by adding sugars like corn or cane sugar, and indirect adulteration by feeding bees adulterating substances. It outlines various detection tests for different types of adulteration, such as testing density for sugar solutions or using Fiehe's test and HMF content to detect invert sugar. The health effects of adulteration and pesticide residues in honey are also mentioned. Finally, the document lists several journal articles and references on topics related to honey adulteration detection and analysis.
Development and validation of GC-MS method for analysis of chloropyramine hyd...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
1) Malaria is a fatal disease caused by Plasmodium parasites and transmitted via the bites of infected Anopheles mosquitoes.
2) Primaquine, an 8-aminoquinoline drug, is commonly used to treat malaria despite side effects, but absorption can be increased using a nanoemulsion formulation.
3) The document discusses developing a primaquine nanoemulsion using castor oil via low energy methods, evaluating batches based on parameters like particle size, pH, and zeta potential, and selecting an optimized stable formulation for further studies.
EVALUATION OF AQUAFEED INGREDIENTS AND FEED QUALITYsouravfnftmb306
This document discusses methods for evaluating aquafeed ingredients and feed quality. It covers biological, physical, and chemical evaluation methods. Physical evaluation involves assessing attributes like smell, taste, and structure using senses and microscopy. Chemical evaluation consists of proximate analysis to determine components like moisture, ash, protein, fat, fiber. Additional chemical analyses include amino acid profiles, lipid quality tests, and vitamin analysis using techniques like HPLC. Biological evaluation parameters monitored during feeding trials include growth rates, feed conversion ratio, digestibility, and protein utilization measures. The choice of high quality feed ingredients is important for aquaculture success, and thorough evaluation using integrated physical, chemical, and biological methods allows for an effective feed formulation.
This document discusses different analytical techniques for determining biogenic amines:
- Thin-layer chromatography was found to effectively separate and determine biogenic amines with repeatability of 1.82% using a specific solvent system.
- Gas chromatography separates components through a temperature controlled column based on chemical and physical properties.
- Capillary electrophoresis uses an electric field to drive charged analytes through a narrow capillary tube to separate biogenic amines in food.
- High-performance liquid chromatography is widely used and can sensitively, specifically, and precisely separate and quantify active ingredients using interactions with a stationary phase and solvent.
Application of GC-MS in Quantitative Analysis of Some Carminative Syrupsiosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
This document describes the development and validation of a reversed phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of aminocaproic acid in pharmaceutical dosage forms. The method was developed using a C18 column with a mobile phase of buffer and methanol. The developed method was validated according to ICH guidelines and found to be precise, accurate, specific, linear and robust for the analysis of aminocaproic acid in drug products and dissolution samples. The method can be used for quality control testing in pharmaceutical laboratories.
Rapid UHPLC Determination of Common Preservatives in Cosmetic Products v2zq
This document presents a fast UHPLC method for quantifying six common parabens (preservatives) in cosmetic products. The UHPLC method using a 1.9 μm column achieved a run time of 3.5 minutes, an over 80% reduction compared to the 19 minute run time using a conventional HPLC column. The UHPLC method also reduced solvent usage by over 90%. The method was shown to be linear, precise, and able to detect parabens at levels within EU and FDA regulations using samples of face lotion and body lotion.
This document discusses honey adulteration, including direct adulteration by adding sugars like corn or cane sugar, and indirect adulteration by feeding bees adulterating substances. It outlines various detection tests for different types of adulteration, such as testing density for sugar solutions or using Fiehe's test and HMF content to detect invert sugar. The health effects of adulteration and pesticide residues in honey are also mentioned. Finally, the document lists several journal articles and references on topics related to honey adulteration detection and analysis.
Development and validation of GC-MS method for analysis of chloropyramine hyd...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
1) Malaria is a fatal disease caused by Plasmodium parasites and transmitted via the bites of infected Anopheles mosquitoes.
2) Primaquine, an 8-aminoquinoline drug, is commonly used to treat malaria despite side effects, but absorption can be increased using a nanoemulsion formulation.
3) The document discusses developing a primaquine nanoemulsion using castor oil via low energy methods, evaluating batches based on parameters like particle size, pH, and zeta potential, and selecting an optimized stable formulation for further studies.
EVALUATION OF AQUAFEED INGREDIENTS AND FEED QUALITYsouravfnftmb306
This document discusses methods for evaluating aquafeed ingredients and feed quality. It covers biological, physical, and chemical evaluation methods. Physical evaluation involves assessing attributes like smell, taste, and structure using senses and microscopy. Chemical evaluation consists of proximate analysis to determine components like moisture, ash, protein, fat, fiber. Additional chemical analyses include amino acid profiles, lipid quality tests, and vitamin analysis using techniques like HPLC. Biological evaluation parameters monitored during feeding trials include growth rates, feed conversion ratio, digestibility, and protein utilization measures. The choice of high quality feed ingredients is important for aquaculture success, and thorough evaluation using integrated physical, chemical, and biological methods allows for an effective feed formulation.
This document discusses different analytical techniques for determining biogenic amines:
- Thin-layer chromatography was found to effectively separate and determine biogenic amines with repeatability of 1.82% using a specific solvent system.
- Gas chromatography separates components through a temperature controlled column based on chemical and physical properties.
- Capillary electrophoresis uses an electric field to drive charged analytes through a narrow capillary tube to separate biogenic amines in food.
- High-performance liquid chromatography is widely used and can sensitively, specifically, and precisely separate and quantify active ingredients using interactions with a stationary phase and solvent.
Application of GC-MS in Quantitative Analysis of Some Carminative Syrupsiosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
This document describes the development and validation of a reversed phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of aminocaproic acid in pharmaceutical dosage forms. The method was developed using a C18 column with a mobile phase of buffer and methanol. The developed method was validated according to ICH guidelines and found to be precise, accurate, specific, linear and robust for the analysis of aminocaproic acid in drug products and dissolution samples. The method can be used for quality control testing in pharmaceutical laboratories.
Development and Validation of Reversed Phase-High-Performance Liquid Chromato...BRNSS Publication Hub
A simple, accurate, precise, and robust in vitro methods developed and validated for measurement of drug release in Aminocaproic Acid tablets. High-performance liquid chromatography (HPLC) method for quantification of drug in dissolution samples of Aminocaproic Acid tablet is developed and validated. 0.1 N Hydrochloric acid is used as dissolution medium and Basket (USP-I) as apparatus at 100 rpm. The sample was withdrawn after 60 min. The developed HPLC method was used for quantitative estimation of drug release in dissolution samples of Aminocaproic Acid tablet. Chromatogram was run through Inertsil ODS 3V, (250 × 4.6 mm), 5 μm. Mobile phase containing buffer solution and methanol in the pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 13.3 g sodium dihydrogen phosphate monohydrate, 500 mg of Heptane-1-sulfonic acid sodium salt, and 1.0 mL of Triethylamine buffer with pH 2.20 adjusted by orthophosphoric acid. Optimized wavelength for Aminocaproic acid was 210 nm. Retention time of Aminocaproic acid was found about 4.0 min; linearity range was 132.605 μg/ml–828.787 μg/ml. The new method was evaluated according to ICH guideline and as far as validation results are concern correlation coefficient value was 0.9999 for the very compound, percentage recovery 100.0%, repeatability results relative standard deviation 0.9 for Aminocaproic acid. The developed HPLC method was found to be a simple and rapid one for regular analysis in professional laboratory.
This document describes the development and validation of a reversed phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of aminocaproic acid in pharmaceutical dosage forms. The method was developed using a C18 column with a mobile phase of buffer and methanol. The developed method was validated according to ICH guidelines and found to be precise, accurate, specific, linear and robust for the analysis of aminocaproic acid in drug products and dissolution samples. The method can be used for quality control testing in pharmaceutical laboratories.
This document describes the development and validation of a reversed phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of aminocaproic acid in pharmaceutical dosage forms. The method was developed using a C18 column with a mobile phase of buffer and methanol. The developed method was validated according to ICH guidelines and found to be precise, accurate, specific, linear and robust for the analysis of aminocaproic acid in drug products and dissolution samples. The method can be used for quality control testing in pharmaceutical laboratories.
Mycotoxins are are secondary metabolites produced by fungi and are dangerous for feed and food chains as they can create contamination in pre- and post-harvest processes. Many are highly toxic and as such levels in food products are regulated in Europe, the US, Japan and other countries. This presentation is an overview of the application of ultra-performance liquid chromatography combined with tandem quadrupole mass spectrometry to analyse various food products for mycotoxins in line with regulatory requirements.
This document discusses chromatography and PCR techniques. It provides details on:
- The principles and types of chromatography including TLC, HPLC, and their components and procedures. HPLC allows for quantitative analysis and is commonly used for pharmaceutical quality control.
- PCR amplification which uses DNA polymerase to exponentially replicate DNA sequences. It requires template DNA, primers, nucleotides, and DNA polymerase. Repeated heating and cooling cycles allow for target DNA replication.
- Applications of chromatography and PCR include pharmaceutical analysis, forensic analysis, detection of genetic disorders, microbial detection, and molecular biology research techniques. Both provide powerful tools for separation, detection, and analysis of biological molecules.
This document describes the development and validation of an RP-HPLC method for the simultaneous estimation of anti-tuberculosis drugs isoniazid, rifampicin, pyrazinamide, and ethambutol in human plasma. It provides background on tuberculosis and the common drugs used to treat it. The document reviews several literature methods for analyzing these drugs and discusses the drug profiles. It states that the objective is to develop a sensitive analytical method to quantitatively determine the drugs and metabolites in biological fluids to evaluate pharmacokinetics and pharmacodynamics.
Multi-residue pesticide analysis of food samples using acetonitrile extractio...Kate?ina Svobodov
This document describes a method for multi-residue pesticide analysis of food samples using two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC-MS/MS). The method involves acetonitrile extraction of samples followed by separation using reverse phase and HILIC columns connected by a switching valve. The method showed improved peak shape and sensitivity for polar pesticides compared to single column methods. Recoveries of 79.67% of analytes were between 60-100% and reporting limits were 1 ppb or lower for most pesticides tested, demonstrating this 2D-LC-MS/MS method is effective for broad-scope pesticide residue testing in foods.
Bulletin of environmental contamination and toxicology sudeb mandalDr Sudeb Mandal
This study evaluated the dissipation of thiophanate methyl residue in grapes grown in India. Field trials were conducted in four locations at two application rates of 500 and 1000 g ai/ha. Samples were collected up to 15 days after application and residues were analyzed. The fungicide dissipated following first order kinetics with half-lives ranging from 4.74 to 6.52 days depending on location and dose. More than 50% of the initial residue degraded within 7 days irrespective of location or treatment. The initial deposits were below India's maximum residue limit of 5 ug/g.
Dissipation Study of Thiophanate Methyl Residue in/on Grapes (Vitis vinifera ...Dr. Sudeb Mandal
A multi-location field trial was conducted in
India during 2006–2008 to evaluate the dissipation pattern
of thiophanate methyl (75% WP) in/on grapes at two
application rates (500 and 1,000 g a.i. ha-1). The quantitative analysis of the fungicide residues as carbendazim
was performed using a UV/VIS spectrophotometer at the
maximum absorption band of 281 nm. The average
recovery was found 87% and the relative standard deviations (RSD) were below 3.8%. Following the first order
kinetics the fungicide dissipates in grapes with a half-life
(t) value of 4.74–6.52 days irrespective of locations and
doses.
Isolation Extraction Estimation of ArtemisininAFSATH
This document summarizes the extraction and quantification of artemisinin from the plant Artemisia annua. The plant material is extracted using solvents like methanol, hexane, and ethyl acetate. The extract is then partitioned and purified through chromatography. Artemisinin content is estimated using two methods - TLC densitometry and HPLC. Both methods involve developing a calibration curve to determine the concentration of artemisinin in the test samples. Artemisinin extracted from A. annua is used effectively to treat malaria and other diseases.
Method Development and Validation on Etomidate injection by RP-HPLCpharmaindexing
This document describes the development and validation of a high performance liquid chromatography (HPLC) method for the analysis of etomidate injection. The method uses a Waters HPLC system with a Develosil-ODS-UG column and a mobile phase of acetonitrile and phosphate buffer at a ratio of 40:60. The method was validated per ICH guidelines and found to be accurate, precise, linear, robust and sensitive for quantifying etomidate in injections. The method was then applied to analyze etomidate levels in marketed injection formulations.
Determination of hydroxy methyl furfural concentration in honey using ultra v...Open Access Research Paper
This paper aimed to determine the concentration of hydroxyl methyl furfural (HMF) using UV-visible spectroscopy to assess the quality of honey. The honey samples were collected from three honeys productive temperature zones: temperate, sub-tropical and tropical. Following the procedure of white method, the concentration of HMF of temperate, sub-tropical and tropical zone honey are found to be 11.18 ± 0.052mg/kg, 24.95± 0.119mg/kg, and 56.94±0.366mg/kg respectively. There is statistically significance differences between the groups in HMF concentration at 95% confidence level (p<0.05). All the samples are found to have HMF value less than the maximum concentration of HMF in honey set by standard controlling international organizations, which shows good quality of the honey in the study areas.
Validation of analytical method for Diabetes Mellitus DhruvkumarPatel25
This document describes the development and validation of an analytical method for the simultaneous estimation of teneligliptin hydrobromide hydrate and metformin hydrochloride in a combined dosage form. It provides background on the drugs, reviews relevant literature, and outlines the methodology that will be used including the stationary and mobile phases, flow rate, detection wavelength, and other analytical conditions. The objective is to develop a stability-indicating method that can accurately quantify both drugs simultaneously.
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION HPLC UVVenkatesh Mantha
The document describes the development and validation of a reverse phase high performance liquid chromatography (RP-HPLC) method for the estimation of drugs in bulk and pharmaceutical dosage forms. It discusses the need for analytical methods in pharmaceutical analysis and introduces RP-HPLC as a commonly used technique. The document provides details of various studies done to develop and validate RP-HPLC methods for estimating specific drugs and drug combinations in research papers. It also provides chemical and pharmacological profiles of drugs that were estimated using RP-HPLC methods discussed in the literature review section. The aim is to develop a validated RP-HPLC method for estimation of a drug in bulk and marketed dosage form.
Solution Preparation automatically calculates and dispenses the exact amount of solvent needed to prepare sample solutions for analytical instruments based on the mass of solute added. It accomplishes this by preparing liquid and gas chromatography samples, spectroscopy samples, and other analyses on a weight-to-weight or molar basis to an accuracy of 0.02%. Typical preparation time is less than 2 minutes, saving labor costs. Up to eight solvents can be dispensed without cross-contamination using disposable sample bottles, eliminating expensive volumetric glassware. It provides documentation and decreases solvent exposure while increasing accuracy and consistency in sample preparation.
Solution Preparation automatically calculates and dispenses the exact amount of solvent needed to prepare sample solutions for analytical instruments based on the mass of solute added. It accomplishes this by weighing samples and solvents to determine solvent volumes to within 0.02% accuracy. Typical preparation times are less than 2 minutes, saving labor costs for labs that analyze many samples daily. It can dispense up to eight solvents without cross-contamination using disposable bottles, eliminating expensive volumetric glassware.
This document describes the development and validation of an HPLC method for the quantification of linagliptin in bulk and pharmaceutical dosage forms. The method was developed using a C18 column with a mobile phase of phosphate buffer and acetonitrile. Standard and sample solutions were prepared and the chromatographic conditions were optimized based on peak symmetry and resolution. The developed method will be validated as per ICH guidelines for specificity, linearity, accuracy, precision, filter compatibility and solution stability. This will provide a stability-indicating assay for the quantification of linagliptin in tablets.
Application of High Performance Thin Layer Chromatography with Densitometry f...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
VALIDATION AND DETERMINATION OF CAFFEINE CONTENT IN ENERGY DRINKS BY USING HP...Ruqsar Fatima
This document outlines the validation and determination of caffeine content in energy drinks using HPLC methods. It includes an introduction on caffeine, the principle of HPLC separation, materials and methods for sample preparation and instrument operation, results and discussion of the validation including calibration curves, precision, accuracy, specificity, LOD and LOQ. The method was found to be precise, accurate, specific and sensitive for quantifying caffeine levels in various energy drinks in under 3 minutes. In conclusion, the validated HPLC method provides an effective quality control technique for caffeine analysis in energy drinks.
Development and Validation of Reversed Phase-High-Performance Liquid Chromato...BRNSS Publication Hub
A simple, accurate, precise, and robust in vitro methods developed and validated for measurement of drug release in Aminocaproic Acid tablets. High-performance liquid chromatography (HPLC) method for quantification of drug in dissolution samples of Aminocaproic Acid tablet is developed and validated. 0.1 N Hydrochloric acid is used as dissolution medium and Basket (USP-I) as apparatus at 100 rpm. The sample was withdrawn after 60 min. The developed HPLC method was used for quantitative estimation of drug release in dissolution samples of Aminocaproic Acid tablet. Chromatogram was run through Inertsil ODS 3V, (250 × 4.6 mm), 5 μm. Mobile phase containing buffer solution and methanol in the pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 13.3 g sodium dihydrogen phosphate monohydrate, 500 mg of Heptane-1-sulfonic acid sodium salt, and 1.0 mL of Triethylamine buffer with pH 2.20 adjusted by orthophosphoric acid. Optimized wavelength for Aminocaproic acid was 210 nm. Retention time of Aminocaproic acid was found about 4.0 min; linearity range was 132.605 μg/ml–828.787 μg/ml. The new method was evaluated according to ICH guideline and as far as validation results are concern correlation coefficient value was 0.9999 for the very compound, percentage recovery 100.0%, repeatability results relative standard deviation 0.9 for Aminocaproic acid. The developed HPLC method was found to be a simple and rapid one for regular analysis in professional laboratory.
This document describes the development and validation of a reversed phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of aminocaproic acid in pharmaceutical dosage forms. The method was developed using a C18 column with a mobile phase of buffer and methanol. The developed method was validated according to ICH guidelines and found to be precise, accurate, specific, linear and robust for the analysis of aminocaproic acid in drug products and dissolution samples. The method can be used for quality control testing in pharmaceutical laboratories.
This document describes the development and validation of a reversed phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of aminocaproic acid in pharmaceutical dosage forms. The method was developed using a C18 column with a mobile phase of buffer and methanol. The developed method was validated according to ICH guidelines and found to be precise, accurate, specific, linear and robust for the analysis of aminocaproic acid in drug products and dissolution samples. The method can be used for quality control testing in pharmaceutical laboratories.
Mycotoxins are are secondary metabolites produced by fungi and are dangerous for feed and food chains as they can create contamination in pre- and post-harvest processes. Many are highly toxic and as such levels in food products are regulated in Europe, the US, Japan and other countries. This presentation is an overview of the application of ultra-performance liquid chromatography combined with tandem quadrupole mass spectrometry to analyse various food products for mycotoxins in line with regulatory requirements.
This document discusses chromatography and PCR techniques. It provides details on:
- The principles and types of chromatography including TLC, HPLC, and their components and procedures. HPLC allows for quantitative analysis and is commonly used for pharmaceutical quality control.
- PCR amplification which uses DNA polymerase to exponentially replicate DNA sequences. It requires template DNA, primers, nucleotides, and DNA polymerase. Repeated heating and cooling cycles allow for target DNA replication.
- Applications of chromatography and PCR include pharmaceutical analysis, forensic analysis, detection of genetic disorders, microbial detection, and molecular biology research techniques. Both provide powerful tools for separation, detection, and analysis of biological molecules.
This document describes the development and validation of an RP-HPLC method for the simultaneous estimation of anti-tuberculosis drugs isoniazid, rifampicin, pyrazinamide, and ethambutol in human plasma. It provides background on tuberculosis and the common drugs used to treat it. The document reviews several literature methods for analyzing these drugs and discusses the drug profiles. It states that the objective is to develop a sensitive analytical method to quantitatively determine the drugs and metabolites in biological fluids to evaluate pharmacokinetics and pharmacodynamics.
Multi-residue pesticide analysis of food samples using acetonitrile extractio...Kate?ina Svobodov
This document describes a method for multi-residue pesticide analysis of food samples using two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC-MS/MS). The method involves acetonitrile extraction of samples followed by separation using reverse phase and HILIC columns connected by a switching valve. The method showed improved peak shape and sensitivity for polar pesticides compared to single column methods. Recoveries of 79.67% of analytes were between 60-100% and reporting limits were 1 ppb or lower for most pesticides tested, demonstrating this 2D-LC-MS/MS method is effective for broad-scope pesticide residue testing in foods.
Bulletin of environmental contamination and toxicology sudeb mandalDr Sudeb Mandal
This study evaluated the dissipation of thiophanate methyl residue in grapes grown in India. Field trials were conducted in four locations at two application rates of 500 and 1000 g ai/ha. Samples were collected up to 15 days after application and residues were analyzed. The fungicide dissipated following first order kinetics with half-lives ranging from 4.74 to 6.52 days depending on location and dose. More than 50% of the initial residue degraded within 7 days irrespective of location or treatment. The initial deposits were below India's maximum residue limit of 5 ug/g.
Dissipation Study of Thiophanate Methyl Residue in/on Grapes (Vitis vinifera ...Dr. Sudeb Mandal
A multi-location field trial was conducted in
India during 2006–2008 to evaluate the dissipation pattern
of thiophanate methyl (75% WP) in/on grapes at two
application rates (500 and 1,000 g a.i. ha-1). The quantitative analysis of the fungicide residues as carbendazim
was performed using a UV/VIS spectrophotometer at the
maximum absorption band of 281 nm. The average
recovery was found 87% and the relative standard deviations (RSD) were below 3.8%. Following the first order
kinetics the fungicide dissipates in grapes with a half-life
(t) value of 4.74–6.52 days irrespective of locations and
doses.
Isolation Extraction Estimation of ArtemisininAFSATH
This document summarizes the extraction and quantification of artemisinin from the plant Artemisia annua. The plant material is extracted using solvents like methanol, hexane, and ethyl acetate. The extract is then partitioned and purified through chromatography. Artemisinin content is estimated using two methods - TLC densitometry and HPLC. Both methods involve developing a calibration curve to determine the concentration of artemisinin in the test samples. Artemisinin extracted from A. annua is used effectively to treat malaria and other diseases.
Method Development and Validation on Etomidate injection by RP-HPLCpharmaindexing
This document describes the development and validation of a high performance liquid chromatography (HPLC) method for the analysis of etomidate injection. The method uses a Waters HPLC system with a Develosil-ODS-UG column and a mobile phase of acetonitrile and phosphate buffer at a ratio of 40:60. The method was validated per ICH guidelines and found to be accurate, precise, linear, robust and sensitive for quantifying etomidate in injections. The method was then applied to analyze etomidate levels in marketed injection formulations.
Determination of hydroxy methyl furfural concentration in honey using ultra v...Open Access Research Paper
This paper aimed to determine the concentration of hydroxyl methyl furfural (HMF) using UV-visible spectroscopy to assess the quality of honey. The honey samples were collected from three honeys productive temperature zones: temperate, sub-tropical and tropical. Following the procedure of white method, the concentration of HMF of temperate, sub-tropical and tropical zone honey are found to be 11.18 ± 0.052mg/kg, 24.95± 0.119mg/kg, and 56.94±0.366mg/kg respectively. There is statistically significance differences between the groups in HMF concentration at 95% confidence level (p<0.05). All the samples are found to have HMF value less than the maximum concentration of HMF in honey set by standard controlling international organizations, which shows good quality of the honey in the study areas.
Validation of analytical method for Diabetes Mellitus DhruvkumarPatel25
This document describes the development and validation of an analytical method for the simultaneous estimation of teneligliptin hydrobromide hydrate and metformin hydrochloride in a combined dosage form. It provides background on the drugs, reviews relevant literature, and outlines the methodology that will be used including the stationary and mobile phases, flow rate, detection wavelength, and other analytical conditions. The objective is to develop a stability-indicating method that can accurately quantify both drugs simultaneously.
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION HPLC UVVenkatesh Mantha
The document describes the development and validation of a reverse phase high performance liquid chromatography (RP-HPLC) method for the estimation of drugs in bulk and pharmaceutical dosage forms. It discusses the need for analytical methods in pharmaceutical analysis and introduces RP-HPLC as a commonly used technique. The document provides details of various studies done to develop and validate RP-HPLC methods for estimating specific drugs and drug combinations in research papers. It also provides chemical and pharmacological profiles of drugs that were estimated using RP-HPLC methods discussed in the literature review section. The aim is to develop a validated RP-HPLC method for estimation of a drug in bulk and marketed dosage form.
Solution Preparation automatically calculates and dispenses the exact amount of solvent needed to prepare sample solutions for analytical instruments based on the mass of solute added. It accomplishes this by preparing liquid and gas chromatography samples, spectroscopy samples, and other analyses on a weight-to-weight or molar basis to an accuracy of 0.02%. Typical preparation time is less than 2 minutes, saving labor costs. Up to eight solvents can be dispensed without cross-contamination using disposable sample bottles, eliminating expensive volumetric glassware. It provides documentation and decreases solvent exposure while increasing accuracy and consistency in sample preparation.
Solution Preparation automatically calculates and dispenses the exact amount of solvent needed to prepare sample solutions for analytical instruments based on the mass of solute added. It accomplishes this by weighing samples and solvents to determine solvent volumes to within 0.02% accuracy. Typical preparation times are less than 2 minutes, saving labor costs for labs that analyze many samples daily. It can dispense up to eight solvents without cross-contamination using disposable bottles, eliminating expensive volumetric glassware.
This document describes the development and validation of an HPLC method for the quantification of linagliptin in bulk and pharmaceutical dosage forms. The method was developed using a C18 column with a mobile phase of phosphate buffer and acetonitrile. Standard and sample solutions were prepared and the chromatographic conditions were optimized based on peak symmetry and resolution. The developed method will be validated as per ICH guidelines for specificity, linearity, accuracy, precision, filter compatibility and solution stability. This will provide a stability-indicating assay for the quantification of linagliptin in tablets.
Application of High Performance Thin Layer Chromatography with Densitometry f...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
VALIDATION AND DETERMINATION OF CAFFEINE CONTENT IN ENERGY DRINKS BY USING HP...Ruqsar Fatima
This document outlines the validation and determination of caffeine content in energy drinks using HPLC methods. It includes an introduction on caffeine, the principle of HPLC separation, materials and methods for sample preparation and instrument operation, results and discussion of the validation including calibration curves, precision, accuracy, specificity, LOD and LOQ. The method was found to be precise, accurate, specific and sensitive for quantifying caffeine levels in various energy drinks in under 3 minutes. In conclusion, the validated HPLC method provides an effective quality control technique for caffeine analysis in energy drinks.
Semelhante a Extraction of Cytotoxic and Carcinogenic compound: HMF (5-hydroxymethylfurfural) from Honey and its Quantification using HPLC (20)
Heritage Conservation.Strategies and Options for Preserving India HeritageJIT KUMAR GUPTA
Presentation looks at the role , relevance and importance of built and natural heritage, issues faced by heritage in the Indian context and options which can be leveraged to preserve and conserve the heritage.It also lists the challenges faced by the heritage due to rapid urbanisation, land speculation and commercialisation in the urban areas. In addition, ppt lays down the roadmap for the preservation, conservation and making value addition to the available heritage by making it integral part of the planning , designing and management of the human settlements.
Extraction of Cytotoxic and Carcinogenic compound: HMF (5-hydroxymethylfurfural) from Honey and its Quantification using HPLC
1. Extraction of
Cytotoxic and
Carcinogenic
compound: HMF
(5-hydroxymethyl
furfural) from
Honey and its
Quantification
using HPLC
Authors:
1Pranjali Singh,
Senior Research Fellow,
CCRAS-RARI, Gwalior
Email: pranjali7888@gmail.com
OP - 31
2. • Honey is a natural product which is
produced by honey bees (Apis mellifera).
• Honey’s composition differs depending on
its floral, geographical and entomological
sources.
• HMF concentration in fresh honey is absent
but its concentration increases during
processing, upon storage for longer periods
of time and upon heating/storing in higher
temperatures.
• HMF contributes to sometimes fatal effects
(mutagenic, chromosomal aberrations,
genotoxic, organotoxic, carcinogenic,
cytotoxicity towards mucous membranes,
the skin, the eyes and the upper respiratory
tract).
3. • Honey is used in the treatment of
Cancer.
• Honey is useful for the Skin as it
gives an anti-oxidant effect,
promotes the development of new
tissue, cures wounds, acne, eczema,
dandruff, tinea and psoriasis as it is
anti-inflammatory, anti-fungal and
anti-bacterial properties.
5. • In this research work, Extraction of HMF (5-
hydroxymethyl furfural) from honey was done and the
amount was estimated by using the reference standard of
HMF on HPLC.
• Honey samples were received from two companies and
the reference standard was obtained from Sigma-Aldrich.
• Extraction of HMF from honey was carried out by
making Carrez I and Carrez II solutions.
• An accurate and specific HPLC method was developed.
6. Extraction of HMF from Honey:
5 g of Honey in 50 ml Volumetric flask
+
25 ml of Water
+
0.6 ml Carrez I Solution ---------------> Filtrate into
Filter HPLC vials
+
0.6 ml of Carrez II Solution
+
make up upto the mark with Water
7. Parameters Chromatographic conditions
Mobile Phase Acetonitrile and Water with 0.2% Formic acid
Chomatographic Column (Stationary phase) Phenomenex C18; 150mm x 4.6 mm x 3 μm
Flow rate 0.6 ml/minute
Column temperature 30°C
Run time 15 minutes
Injection volume 10 μl
Wavelength 282 nm
Elution Mode Isocratic
Chromatographic
conditions:
8. Run time
(minutes)
Mobile phase A
(Water with 0.2% Formic acid)
Mobile phase B
(Acetonitrile)
15 90 10
Mobile phase composition
9. Standard Stock Solutions and Calibration Standards
• Standard Stock Solution was prepared with 2 mg/ml Concentration.
• The Calibration Standards 0.012 ppm, 0.024 ppm, 0.049 ppm, 0.098 ppm, 0.195 ppm, 0.391 ppm, 0.780
ppm, 1.563 ppm, 3.125 ppm, 6.25 ppm were prepared by ½ Serial Dilutions of Standard Stock Solution.
• These standards/points were used to plot on the Calibration curve of HMF Reference Standard.
• The Calibration plot showed a linear calibration curve with a correlation coefficient, R2 value of 0.999.
• The retention time of the HMF Reference standard was 6.53 minutes.
10. • The amount of HMF present in Sample 1 and Sample 2 was found to be 3.80 mg/kg and 6.50 mg/kg
respectively.
• The amount of HMF in honey in the samples were found to be within the limit of 40 mg/kg as per FSSAI.