Anticancer Drug Screening ‘ Cancer’: a fatal disease of uncontrolled proliferation of genetically altered cells. In all known cases, cancer cells are derived from the repeated divisions of a mutant cell. Some of these mutations may be due to the effects of carcinogens, such as tobacco smoke, radiation, chemicals or infectious agents. A few other cancer-promoting mutations may be acquired through errors in DNA replication. Genetic alterations that render a normal cell cancerous usually arise in two classes of genes termed the oncogenes and tumors suppressors. Activate the cancer promoting oncogenes and/or inactivate the tumor suppressor genes. Cancer

Figure-1: Normal (Control) HeLa cells showing no signs of necrosis or apoptosis Figure-2: Effect of Doxorubicin on HeLa cells showing cell shrinkage, nuclear condensation Figure-3: Effect of compound - 6 on HeLa cells showing apoptosis and cell necrosis Figure-4: Effect of Com -4 on HeLa cells showing cell shrinkage and nuclear condensation

Cell Cycle Analysis Control R6 R3 R4 R5 DOX R6 R3 R4 R5 Phase Sub-G 0 G 0 -G1 S G 2 -M Phase Sub-G 0 G 0 -G 1 S G 2 -M % cells 1.15 74.27 6.34 18.65 % cells 2.22 16.62 4.01 76.76 Figure-7: Control HeLa cells showing normal cell cycle Figure-8: Doxorubicin showing prominent G 2 -M phase arrest

BE-7 R5 R2 R3 R4 BE-4 R6 R3 R4 R5 Phase Sub-G 0 G 0 -G 1 S G 2 -M Phase Sub-G 0 G 0 -G 1 S G 2- M % cells 27.87 44.46 9.73 17.91 % cells 0.62 88.41 1.04 9.72 Figure-9: Com-6 treated HeLa cells showing sub G 0 (apoptotic) arrest Figure-10: Com-4 treated HeLa cells showing prominent G 0 -G 1 phase arrest

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