(July 21, 2021) Webinar #3 Applications Review – Highlighting Imaging Examples Using the IVM Intravital Microscope
•Transferir como PPTX, PDF•
0 gostou•94 visualizações
After introducing intravital imaging in Webinar #1 and the IVM system and its capabilities in Webinar #2, we brought you real-world examples of IVM applications in collaboration with our reference sites. We presented specific examples of how to apply the IVM system to address various research questions during this webinar. After viewing this on-demand webinar, attendees will have an understanding of how the IVM system can be applied in preclinical research to visualize and analyze in vivo dynamic processes at a cellular level. Please register for the final part of this webinar series to see the IVM system in action as we give a live virtual demonstration of the IVM system and showcase its software and hardware components.
Recomendados
Recomendados
Mais conteúdo relacionado
Mais procurados
Mais procurados (20)
Semelhante a (July 21, 2021) Webinar #3 Applications Review – Highlighting Imaging Examples Using the IVM Intravital Microscope
Semelhante a (July 21, 2021) Webinar #3 Applications Review – Highlighting Imaging Examples Using the IVM Intravital Microscope (20)
Mais de Scintica Instrumentation
Mais de Scintica Instrumentation (20)
Último
Último (20)
(July 21, 2021) Webinar #3 Applications Review – Highlighting Imaging Examples Using the IVM Intravital Microscope
- 1. Applications Review: Highlighting Imaging Examples Using the IVM Intravital Microscope DR. PILHAN KIM Associate Professor, Korea Advanced Institute of Science and Technology, Daejeon, Korea CEO, IVIM Technology, Inc., Daejeon, Korea DR. KEEHOON JUNG Assistant Professor, Seoul National University College of Medicine, Seoul, Korea
- 3. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell Imaging X-ray / CT / MRI (Tissue-level) Intravital Microscopy (Cell-level, Video) Sepsis lung Bloodcirculation Neutrophil provide a detailed live video of disease development in cellular-level Eur. Res. J. 2019,53:1800736
- 4. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell Imaging Macroscopic-scale Imaging (Oran/Tissue-level: StructuralInformation) Microscopic-scale Imaging & Analysis (3D Cell-level: Molecular/FunctionalInformation) Intravital microscopy (IVM) Islet cell (MIP-GFP) Vessel (CD31) PET / CT / MRI X-Ray OCT / OFDI Ultrasound Cancercell (B16F10-GFP) Vessel (CD31) Sinusoid (LYVE-1) Blood circulation (FITC-Dextran) Neutrophil (Ly6G) IVIM’s IntraVitalMicroscopy(IVM) enablesa dynamic3D imagingof various cellular-leveldynamics such as cell trafficking,cell-cellinteraction,and cell- microenvironmentinteractioninside the living preclinicalanimalmodelin vivo. For drug development,IVM enablesa direct imaging analysis,monitoring the drug deliveryto target tissue and cell, assessingthe drug efficacy,and validatingthe mode of action (MOA) in various preclinicalmodelsof humandiseases.
- 5. Copyright©IVIMTechnology.Allrightreserved2021 Skin Inflammatory response KARS Granulocyte Bone Marrow Transplanted BM cell, HSPC Lung Microcirculation in Sepsis Model Neutrophil Blood flow BM cell H2B Sinus IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell Imaging IVIM’s IntraVitalMicroscopy(IVM) enablesa dynamic3D imagingof various cellular-leveldynamics such as cell trafficking,cell-cellinteraction,and cell- microenvironmentinteractioninside the living preclinicalanimalmodelin vivo. For drug development,IVM enablesa direct imaging analysis,monitoring the drug deliveryto target tissue and cell, assessingthe drug efficacy,and validatingthe mode of action (MOA) in various preclinicalmodelsof humandiseases.
- 6. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell Imaging Cancer Xenograft - T cell Triple Negative Human Breast Cancer Drug Delivery - Nanoparticle Anti-cancer nanoparticle delivery T cell Cancer cell Vessel Drug carrier Cancer cell Vessel Drug Distribution - Nanoparticle Real-time nanoparticle delivery Nanoparticle Liver sinusoid IVIM’s IntraVitalMicroscopy(IVM) enablesa dynamic3D imagingof various cellular-leveldynamics such as cell trafficking,cell-cellinteraction,and cell- microenvironmentinteractioninside the living preclinicalanimalmodelin vivo. For drug development,IVM enablesa direct imaging analysis,monitoring the drug deliveryto target tissue and cell, assessingthe drug efficacy,and validatingthe mode of action (MOA) in various preclinicalmodelsof humandiseases.
- 7. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell Imaging
- 8. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – IVM C/M/CM/MS
- 9. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – IVM C/M/CM/MS
- 10. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – IVM C/M/CM/MS Real-time Intravital Microscopy (Video-rate imaging)
- 11. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – IVM C/M/CM/MS Real-time Intravital Microscopy (Video-rate imaging) No Motion Compensation Motion Compensation
- 12. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell Imaging
- 13. Singer et al. JAMA, (2016) Sepsis Lagu T et al. Crit Care Med (2012) Wang HE et al. Crit Care Med (2017)
- 14. Intravital Microscopy Stabilized Suction Chamber Imaging Window Lung Stabilized lung imaging - Suction-assisted lung stabilization imaging window chamber Cover glass Mouse Intubation Imaging chamber holder Tilting mount Lung Lung Imaging Window chamber Suction tube Suction hole
- 15. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology: Intravital Microscopy (IVM) – In Vivo Live Cell Imaging https://youtu.be/IP-CfUjHP4E Lung Surgery for Stabilized IntraVital Imaging Intravital Microscopy of Lung (Blood Vessel,Neutrophil,Circulation)
- 16. Tie2-GFP RBC-DiD Tie2-GFP Donor DiD Staining Adoptive transfer Recipient Tie2-GFP Real-time Intravital Lung Imaging RBC DiD-RBC European Respiratory Journal 2019,53:1800736
- 17. Tie2-GFP RBC-DiD 50μm XY Motion Compensation - SNR improvement Y X European Respiratory Journal 2019,53:1800736
- 18. Track Speed Mean (μm/sec) 0 1500 Tie2-GFP RBC-DiD 50μm European Respiratory Journal 2019,53:1800736 50μm
- 19. Tie2-GFP RBC-DiD 50 μm PBS LPS – Sepsis Model 50 μm European Respiratory Journal 2019,53:1800736
- 20. Tie2-GFP RBC-DiD European Respiratory Journal 2019,53:1800736 T Y X 00:00 00:10 00:20 00:30 Time Projection (900 frames / 30 sec)
- 21. PBS LPS Erythrocyte Merge Capillary Tie2 DiD-RBC Tie2 DiD-RBC * * * * * * * * * * * * * * * European Respiratory Journal 2019,53:1800736 Disturbance of Pulmonary Microcirculation in Acute Lung Injury
- 22. FITC-Dextran Neutrophil-Ly6G NeutrophilsDisturbPulmonary MicrocirculationinSepsis- inducedAcuteLungInjury Eur.Resp.J.53:1800736(2019) Neutrophil Cluster Formation in Pulmonary Microcirculation 10:00 00:00 04:00 07:00
- 23. Dead-Space Formation by Neutrophil Cluster FITC-Dextran Neutrophil-Ly6G RBC-DiD Eur.Resp.J.53:1800736(2019)
- 24. 100 μm Dead-Space Formation by Neutrophil Cluster FITC-Dextran Neutrophil-Ly6G RBC-DiD FITC-Dextran Neutrophil-Ly6G RBC-DiD Eur.Resp.J.53:1800736(2019)
- 25. Neutrophil depletion restore pulmonary microcirculation European Respiratory Journal 2019,53:1800736
- 26. I J K L H G Ly6G Merge PBS LPS CD11b Ly6G Merge PBS LPS CD18 European Respiratory Journal 2019,53:1800736 Mac-1 (CD11b/CD18) upregulation in the entrapped neutrophil
- 27. Abciximab (c7E3)
- 28. Mac-1 (CD11b/CD18) inhibition improves pulmonary microcirculation C F Capillary Neutrophil Sham Fc Erythrocyte Merge Anti-CD11b Abciximab FITC Dextran Ly6G DiD-RBC * * * * * * * * * * * * Ly6G DiD-RBC FITC Dextran European Respiratory Journal 2019,53:1800736
- 29. Abciximab improves pulmonary microcirculation in Sepsis-induced ALI E G FITC Dextran Ly6G DiD-RBC Merge Erythrocyte Pre-Abciximab Post-Abciximab DiD-RBC European Respiratory Journal 2019,53:1800736
- 30. Abciximab improves pulmonary microcirculation in Sepsis-induced ALI European Respiratory Journal 2019,53:1800736
- 31. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology | Intravital Microscopy (IVM) – In Vivo Live Cell Imaging
- 32. Copyright©IVIMTechnology.Allrightreserved2021 IVM-MS2: Two-Photon Model, Newly Launched at 2021 32 IVM-MS2 isthe most compact and affordable intravital two-photon microscope in the market • Costandspacesaving,EasyinstallationandHighusability,Hands-freemaintenance,All-in-onepackage • Fullyintegratedwithacompactfs-pulselasermodulefortwo-photonimagingand4Detectors formulti-colorimaging • Two-photonmicroscopemainboxsizeis500x620x470mm(WxDxH) • Slidingblindtocoversamplestage,alleviatingthespacerequirementofdarkroom
- 33. Copyright©IVIMTechnology.Allrightreserved2021 IVM-MS2: Two-Photon Model, Newly Launched at 2021 33 Adipose Tissue Skeletal Muscle Nuclei Actin Lung Ear Skin Actin SHG Nuclei Actin SHG Nuclei Actin SHG IVM-MS2 isthe most compact and affordable intravital two-photon microscope in the market • Costandspacesaving,EasyinstallationandHighusability,Hands-freemaintenance,All-in-onepackage • Fullyintegratedwithacompactfs-pulselasermodulefortwo-photonimagingand4Detectors formulti-colorimaging • Two-photonmicroscopemainboxsizeis500x620x470mm(WxDxH) • Slidingblindtocoversamplestage,alleviatingthespacerequirementofdarkroom
- 34. Copyright©IVIMTechnology.Allrightreserved2021 IVIM Technology: IntraVital Imaging Service
- 35. Copyright©IVIMTechnology.Allrightreserved2021 Full Product / Service Catalog – Available on your request Webpage: http://www.ivimtech.com Email: information@ivimtech.com
- 36. Copyright©IVIMTechnology.Allrightreserved2021 Webpage: http://www.ivimtech.com Email: information@ivimtech.com
- 37. WWW.SCINTICA.COM Q&A Session WWW.SCINTICA.COM INFO@SCINTICA.COM Please enter your questions in the Q&A section. Thank You