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Automated phylogenetic taxonomy in Fungi. DS. Hibbett
1. Automated Phylogenetic Taxonomy in Fungi
David Hibbett, Henrik Nilsson, Moran Shonfeld, Mario Fonseca, Marc Snyder, Pete Stein, Ryan Twomey,
Janine Costanzo, Brandon Gaytan, J. P. Burke, and Daniel Menard, Clark University,
and Thomas Heider, College of the Holy Cross,
Worcester, Massachusetts USA
2. Uses of comprehensive trees:
Identification
Curation of public sequence databases
Identification of environmental samples
Discovery of new species
Biogeography
Descriptive biogeography—estimation of ranges
Historical biogeography
Epidemiology, plant pathology
Conservation—phylogenetic diversity analyses
Character evolution
Ancestral state reconstruction
Analyses of directionality
Tests of key innovation hypotheses
Uses of classifications:
Communication
Research
Teaching
Legislation
Representation of the history of life
3. I. Progress in phylogenetic reconstruction in Fungi/Agaricomycetes
II. Progress in classification in Agaricomycetes
III. Automated Phylogenetic Taxonomy in Agaricomycetes
IV. Conclusions and future directions
4. I. Progress in phylogenetic reconstruction in Fungi/Agaricomycetes
II. Progress in classification in Agaricomycetes
III. Automated Phylogenetic Taxonomy in Agaricomycetes
IV. Conclusions and future directions
5. Fungal diversity:
Total species of Fungi 1,500,000 (Hawksworth, 1995)
Described species of Fungi 70,000-100,000 (Rossman, 1995)
Described species of Agaricomycetes 20,000 (Kirk et al. 2001)
Fungal sequence data in GenBank:
Fungi
Core nucleotide seqs 631,464
No. unique names including uncultured, unspecified 32,766
No. spp. excluding uncultured, unspecified 17,131 (17-24% of described spp.; 1% of extant spp.)
Agaricomycetes
Core nucleotide seqs 38,589
No. unique names including uncultured, unspecified 8,061
No. spp. excluding uncultured, unspecified 4,842 (24% of described spp.)
Nuc-lsu rDNA sequences > 800 bp 3,940
ITS rDNA sequences 13,905
ID to species
No. new names
All names
Deposited
(Fungi)
93 94 95 96 97 98 99 00 01 02 03 04 05 06 07
7. LABS/PEOPLE
5 labs (Hibbett/Clark University; Lutzoni & Vilgalys/Duke
University; McLaughlin/Univ. of Minnesota;
Spatafora/Oregon State Univ.
8 post-docs
12 Ph.D. students
26 visiting students and scholars
17 undergraduates
DATA
5191 sequences, 7 genes, 2087 species
41 subcellular characters, 30 species
http://aftol.org/
PUBLICATIONS
49 articles in print, 14 in press/in review
INFORMATICS
WASABI: http://www.lutzonilab.net/aftol/
mor: http://mor.clarku.edu/
Structural and Biochemical Database: http://aftol.umn.edu/
OUTREACH
Workshops for teachers
Teaching the Fungal Tree of Life website
http://www.clarku.edu/faculty/dhibbett/TFTOL/index.html
8. T. James et 69 al.
199 Fungi
15 non-fungi
6436 bp: rpb1,
bp:
rpb2, tef1-α, nuc-
tef1-α
lsu rDNA, nuc-ssu
rDNA,
rDNA, nuc 5.8s
rDNA,
rDNA
9. 1078 Neur ospora crassa S
216 Sordaria fimicola S
1085 Podospora anser ina S
phagotroph to Ascomycota 217 Chaetomium globosum S
H
T. James et 69 al. 1081 Magnaporthe grisea P
phototroph
O
1087 Copr inopsis cinerea S 935 Diapor the er es P
lichenized
L
480 Lycoper don pyrifor me S 952 Gnomonia gnomon P
mycorrhizal
M
626 C opr inus comatus S 51 Xylaria hypoxylon S
plant pathogen 563 C lavar ia zollingeri ?
P
63 Xylaria acuta S
100/100
animal pathogen 673 Amanita brunnescens M
A
1082 F usarium gr aminearum P
625 Pluteus romellii S
mycoparasi te 161 Fusarium aff. solani P
Y
285 Cortinarius iodes M
insect commensual 186 Hydropisphaer a erubescens S
199 Fungi
I
564 Pleurotus ostreatus S 52 Hypocrea citr ina Y
saprobe
S
449 Armillar ia mellea S P M 914 Microascus tr igonospor us S
uncertain
?
558 Flammulina velutipes S 413 Lindra thalassiae S
556 Marasmius alliaceus S
15 non-fungi 424 Lulworthia gr andispora S
genome sequenced 542 Ampulloclitocybe clavipes ? 1 Leotia lubrica S
557 Collybia tuberosa S
motile cell stage present 147 Coccomyces dentatus S
468 Henningsomyces candidus S 744 Potebniamyces pyri P
729 Hygrocybe aff. conica S
phagotroph
151 Chlorociboria aer uginosa S
phototroph 439 Calostoma cinnabar inum M 76 Mollisia cinerea P
100/71 713 Boletellus projectellus M
mutualist
279 Monilinia fr ucticola P Ascomycota :
pathogen 714 Hygrophor opsis aurantiaca S 59 Botryotinia fuckeliana P Leotiomycetes
6436 bp: rpb1,
saprobe
bp: 717 Suillus pictus M 941 Dermea acerina S
uncertain 576 Fibulorhizoctonia sp. I 166 Cudoniella clavus S
455 Echinodontium tinctorium S 49 Lachnum virgineum S
682 Lactarius deceptivus M 56 Geoglossum nigritum S
rpb2, tef1-α, nuc-
tef1-α 452 Bondarzewia montana S 64 Trichoglossum hirsutum S
Basidiomycota: 492 Stereum hirsutum S 1004 Pleopsidium chlor ophanum L
Agaricomycotina 447 C oltricia perennis M 1005 Acarospora schleicheri L
688 Fomitipor ia mediterranea S P 1007 Acarospora laqueata L
lsu rDNA, nuc-ssu
rDNA, 484 Phlebia radiata S 106 Echinoplaca str igulacea L
767 Climacodon septentr ionalis S 958 Diploschistes ocellatus L
776 Phanerochaete chrysosporium S 78 Acarosporina microspora S
562 Grifola sordulenta S P 398 Stictis radiata S
rDNA, nuc 5.8s
rDNA, 100/100 701 Grifola fr ondosa S P 296 Orceolina ker guelensis L
770 Fomitopsis pinicola S P 962 Trapelia placodioides L
518 Hyphoderma praetermissum S 224 Pertusaria dactylina L
100/92 Asco mycota :
700 Cotylidia sp. ? 358 Dibaeis baeomyces L
rDNA Lecanor omycetes
466 Gautieria otthii M 645 Umbilicar ia mammulata L
100/73 724 Ramaria rubella M 687 Hypocenomyce scalaris L
471 Hydnum albomagnum M 134 Peltigera degenii L
438 Calocera cornea S 196 Mycoblastus sanguinarius L
454 Dacryopinax spathularia S 639 Lecanora hybocar pa L
1088 Cryptococcus neoformans A 6 Canoparmelia caroliniana L
505 Ustilago maydis P 3 Cladonia car oliniana L
Basidiomycota: 867 Cintractia sorghi vulgaris P 642 Bacidia schweinitzii L
Ustilaginomycotina 870 Tilletiopsis sp. P 84 Physcia aipolia L
865 Tilletiar ia anomala P 1079 Aspergillus fumigatus S A
100/80 675 Agaricostilbum hyphaenes S 1080 Aspergillus nidulans S
709 Colacogloea peniophorae Y 426 Monascus purpureus S
Basidiomycota:
674 Rhodotorula hor dea S P 1083 Histoplasma capsulatum A
Urediniomycotina 456 Endocronartium harknessii P 1084 Coccidioides immitis A
1459 Puccinia graminis P 430 Spiromastix warcupii S
710 Platygloea disciformis Y 657 Capronia pilosella S
138 Scutellospora heter ogama M 668 Exophiala dermatitidis S A
100/81
139 Glomus mosseae M 659 Ramichlor idium anceps S
Glomeromycota 845 Glomus intraradices M 669 Exophiala pisciphila S A
574 Geosiphon pyriformis M 684 Agonimia sp. L L
844 Paraglomus occultum M 91 Dermatocar pon miniatum L
* 141 Mortierella verticillata S 661 Endocarpon pallidulum L
144 Umbelopsis ramanniana S 697 Staurothele fr ustulenta L
184 Phycomyces blakesleeanus S 342 Pyrgillus javanicus L
1241 Rhizopus or yzae S 387 Pyrenula pseudobufonia L
539 Endogone pisiformis S 891 Peltula umbilicata L Ascomycota :
“Z ygomycota” 136 Dimargaris bacillispora Y 892 Peltula auriculata L Lichinomycetes
140 Coemansia reversa S 896 Lichinella iodopulchra L
1062 Orphella aff. haysii I 101 Anisomeridium polypor i L
29 Smittium culisetae I 1036 Trematosphaeria heter ospora S
185 Spiromyces aspiralis S 1037 Westerdykella cylindrica S Ascomycota:
142 Rhopalomyces elegans A 283 Pyrenophora phaeocomes P Dothi deom ycet es
145 Piptocephalis corymbifera Y
--/85 54 Cochliobolus heterostr ophus P
301 Basidiobolus ranarum S A 940 Pleospora herbarum S
“Chytri diomycota” 633 Olpidium brassicae P 110 Trypethelium sp. L
28 Entomophthora muscae A 274 Dothidea sambuci P
“Z ygomycota” Ascomycota :
137 Conidiobolus cor onatus S A 921 Dothidea insculpta S Dothideomycetes
19 Physoderma maydis P 939 Capnodium coffeae P
18 Coelomomyces stegomyiae A 355 Dendr ographa minor L A scomycota:
300 Allomyces arbusculus S 126 Roccella fuciformis L
80 Simonyella variegata L A rthoniomycetes
20 Rhizoclosmatium sp. S
24 Polychytrium aggregatum S 148 Cheilymenia stercor ea S
27 Cladochytrium replicatum S 62 Scutellinia scutellata S
21 Batrachochytr ium dendr obatidis A 100/94
“Chytridiomycota” 65 Aleur ia aurantia S
689 Rhizophydium macroporosum S 949 Pyronema domesticum S
43 Rhizophlyctis rosea S 50 Sarcoscypha coccinea S
182 Spizellomyces punctatus S 152 Caloscypha fulgens S Ascomycota :
635 Synchytr ium macrosporum P 176 Gyromitr a californica S Pezizomycetes
25 Monoblepharella sp. S 179 Disciotis sp. S
26 H yalor aphidium curvatum S 60 Morchella aff. esculenta S
638 Neocallimastix sp. ? 66 Helvella compr essa S
1068 Encephalitozoon cuniculi A
181 Ascobolus crenulatus S
Microspor idia
1089 Antonospora locustae A 507 Peziza vesiculosa S
297 Rozella allomycis Y
“Chytri diomycota” 100/93 71 Peziza pr oteana S
Caenorhabditis elegans H 905 Orbilia vinosa S Ascom ycota: Orbiliomycetes
Ciona intestinalis H 906 Orbilia auricolor S
Metazoa Homo sapiens H 1069 Saccharomyces cer evisiae S
Drosophila melanogaster H 1070 Saccharomyces castellii S
Choanoflagellida Monosiga brevicollis H 1073 Candida glabrata A
Mycetozoa Dictyostelium discoideum H 1071 Kluyveromyces waltii S
C ryptospor idium parvum A
Apicomplexa 1072 Ashbya gossypii P
100/94 Ascomycota:
Toxoplasma gondii A 1075 Kluyveromyces lactis S
Phytophthora sojae P Saccharomycotina
1074 Candida albicans A
Stramenopiles
Thalassiosira pseudonana O to basal 1269 C andida tropicalis A
Rhodophyta Cyanidioschyzon merolae O 1270 Candida guilliermondii A
lineages
Arabidopsis thaliana O 1077 Debaryomyces hansenii S
Viridiplantae Populus trichocarpa O 1268 Candida lusitaniae A
Oryza sativa O 1076 Yarrow ia lipolytica S
Chlamydomonas reinhar dtii O 1199 Schizosaccharomyces pombe S
Ascomycota:
265 Taphr ina wiesneri P
266 Protomyces inouyei P Taphrinomycotina
100/98
0.05 substitutions/site 1192 Pneumocystis carinii A
10. Mycologia 98(6) 2006:
1. James et al. “Chytridiomycota”
2. White et al. “Zygomycota”
3. Redecker & Rabb Glomeromycota
4. Aime et al. Pucciniomycotina
5. Begerow et al. Ustilaginomycotina
6. Hibbett Agaricomycotina
7. Larsson et al. Hymenochaetales
8. Moncalvo et al. Cantharellales
9. Hosaka et al. Phallomycetidae
10. Miller et al. Russulales
11. Binder & Hibbett Boletales
12. Matheny et al. Agaricales
13. Sugiyama et al. Ascomycota
14. Suh et al. Saccharomycetales
15. Spatafora et al. Pezizomycotina
16. Hansen & Pfister Pezizomycetes
17. Schoch et al. Dothideomycetes
18. Geiser et al. Eurotiomycetes
19. Wang et al. Leotiomycetes
20. Zhang et al. Sordariomycetes
21. Miadlikowska et al. Lecanoromycetes
Papers citing AFTOL
11. P. Matheny et al. MPE 2007.
Data: 146 OTUs, five genes:
nuc-lsu rDNA
nuc-ssu rDNA
nuc 5.8S rDNA
tef1, rpb2
Total: 8671 bp
12. Agaricales
P. Matheny et al. MPE 2007. BP
Boletales
BP
Data: 146 OTUs, five genes: BP
BP
nuc-lsu rDNA
Atheliales
B NA
nuc-ssu rDNA
BP
nuc 5.8S rDNA
tef1, rpb2 Russulales
Total: 8671 bp B
BP
Thelephorales
B BP
Analyses/Support: B
Bayesian analysis Agaricomycetes BP Polyporales
B
of all-nucleotide dataset
B
B = post. prob. = 1.0
B
Hymenochaetales
BP
Parsimony bootstrap of
BP
rDNA nucleotide/protein BP
Corticiales
BP
amino acid dataset:
P = 70-89%
BP
BP
P > 90% Trechisporales
BP
BP
Phallomycetidae
U
BP
st BP
Auriculariales
ila
gi
no
U
BP B
re Cantharellales
di my
c
n
AS om otin
Sebacinales
BP
y a
C
O cot
i
M
YC na BP
Dacrymycetes
O
TA
NA
Tremellomycetes
13. 107 recent studies (1999-2007) on individual clades of Agaricomycetes
Aanen et al. (2000), de Arruda et al. (2003), Binder et al. (2001), Boyle et al. (2006), Callac et al. (2005),
Agaricales
Challen et al. (2003), Chapela and Garbelotto (2004), Coetzee et al. (2000, 2001, 2002, 2003, 2005), Dentinger
et al. (2007), Drehmel et al. (1999), Frøslev et al. (2004, 2004), Garnica et al. (2003), Gulden et al. (2005),
Hofstetter et al. (2002), Høiland and Holst-Jensen (2000), Hopple and Vilgalys (1999), Hughes et al. (2001),
Hwang and Kim (2000), Kerrigan et al. (2005), Kirchmair et al. (2004), Krüger et al. (2001), Martin and
Raccabruna (1999), Mata et al. (2001), Matheny et al. (2002), Mitchell and Bresinsky (1999), Moncalvo et al.
(2000a, 2000b, 2002), Mwenje et al. (2003), Oda et al. (2004), Peintner et al. (2001, 2002, 2003, 2004),
Redhead et al. (2002), Seidl (2000), Thorn et al. (2000), Vellinga (2003, 2004), Wilson et al. (2005), Yang et al.
(2005)
Bakker et al. (2004), Binder and Bresinsky (2002), Bresinksky et al. (1999), Den Bakker et al. (2004), Grubisha
Boletales
et al. (2001, 2002), Jarosch and Besl (2001), Jarosch (2001), Kretzer and Bruns (1999), Kretzer et al. (2003),
Miller (2002, 2003), Peintner et al. (2003), Reddy et al. (2005), Taylor et al. (2006), Binder and Hibbett (2007)
Henkel et al. (2000), E. Larsson et al. (2003), E. Larsson and Hallenberg (2001), Lickey et al. (2002), Miller et
Russulales
al. (2001, 2002), Nuytinck et al. (2004), Wu et al. (1999), Lebel et al. (2004), Miller et al. (2007)
Decock et al. (2005), Fischer and Binder (2004), Geslebin et al. (2004), Paulus et al. (2002), Redberg et al.
Hymenochaetales
(2003), Wagner and Fischer (2001, 2002a, 2002b), Larsson et al. (2007)
Dai et al. (2006), De Koker et al. (2003), Desjardin et al. (2004), Hong et al. (2002), Hong and Jung (2004), Kim
Polyporales
et al. (2005), Ko et al. (2001), Krüger (2004), Nilsson et al. (2003), Wang et al. (2004)
Dahlman et al. (2000), Dunham et al. (2003),Gonzalez et al. (2001), Kottke et al. (2003), Moncalvo et al. (2007)
Cantharellales
Eberhardt et al. (1999), Kernaghan et al. (2002), Lilleskov et al. (2002)
Atheliales
DePriest et al. (2005), Diederich et al. (2003), Matsuura et al. (2000), Sikaroodi et al. (2001)
Corticiales
Kõljalg et al. (2000, 2001, 2002)
Thelephorales
Geml et al. (2005), Humpert et al. (1999), Hosaka et al. (2007)
Phallomycetidae
Weiss et al. (2004), Selosse et al. (2002)
Sebacinales
K.-H. Larsson (2001)
Trechisporales
14. Most inclusive phylogenetic analyses of Agaricomycetes
J. M. Moncalvo et al. 2002. One hundred and seventeen
clades of euagarics (Agaricales)
Data: nuc-lsu rDNA
No. seqs. sampled 877
No. species sampled 877
Nuc-lsu rRNA > 800 bp in GenBank 2309
Species in GenBank 2429
M. Binder et al. 2005. The phylogenetic distribution of
resupinate forms across the major clades of
homobasidiomycetes (Agaricomycetes)
Data: mt/nuc lsu/ssu rDNA
No. seqs. sampled 656
No. species sampled 640
Nuc-lsu rRNA > 800 bp in GenBank 3940
Species in GenBank 4842
M. Binder and D. S. Hibbett. 2006. Molecular systematics
and biological diversification of Boletales.
Data: nuc lsu rDNA
No. seqs. sampled 435
No. species sampled 301
Nuc-lsu rRNA > 800 bp in GenBank 469
Species in GenBank 442
15. I. Progress in phylogenetic reconstruction in Fungi/Agaricomycetes
II. Progress in classification in Agaricomycetes
III. Automated Phylogenetic Taxonomy in Agaricomycetes
IV. Conclusions and future directions
17. quot;The NCBI taxonomy database is not a primary
source for taxonomic or phylogenetic information.
Furthermore, the database does not follow a single
taxonomic treatise but rather attempts to incorporate
phylogenetic and taxonomic knowledge from a
variety of sources, including the published literature,
web-based databases, and the advice of sequence
submitters and outside taxonomy experts.
Consequently, the NCBI taxonomy database is not a
phylogenetic or taxonomic authority and should not
be cited as such.quot;
http://www.ncbi.nlm.nih.gov:80/Taxonomy/taxonomyhome.html
28. Summary: current status of homobasidiomycete systematics
Largely incomplete documentation of extant species
Ca. 20% of described species represented in GenBank
Steady accumulation of “taxonomic” sequences, accelerating accumulation of
“environmental” sequences
Higher-level analyses resolve broad outlines of Agaricomycete phylogeny
A plethora of analyses at lower taxonomic levels
Lack of integration of existing data
Unacceptably slow translation of phylogenies into classifications
A disconnect between phylogenetic reconstruction and classification, creating a
(widening?) gap between taxonomy and understanding of phylogeny
What is needed to achieve a comprehensive, phylogenetically accurate
classification of homobasidiomycetes?
A dramatic increase in the rate of species discovery, including sequence-based
discovery and description
Automated integration of emerging data into comprehensive trees
Automated translation of trees into classifications
29. I. Progress in phylogenetic reconstruction in Fungi/Agaricomycetes
II. Progress in classification in Agaricomycetes
III. Automated Phylogenetic Taxonomy in Agaricomycetes
IV. Conclusions and future directions
37. Heuristic search strategy:
1 2
Load General Constraint as backbone
Create General Constraint tree
monophyly constraint.
(220 species, based on multilocus
Add new sequences to dataset and tree and
analyses).
perform branch swapping (TBR, 5 hours).
Save new tree as Temporary Constraint.
3 4
Load Temporary Constraint as backbone Load General Constraint as backbone.
monophyly constraint. Use (new) Temporary Constraint as a starting
Add new sequences to dataset and tree, but tree for branch swapping (TBR, 5 hours).
perform no swapping. Save new tree as Temporary Constraint.
Save new tree as Temporary Constraint Go to 3.
(i.e., overwrite file).
40. Parsing a node-based phylogenetic taxon definition:
“Taxon X is the least-inclusive clade that contains D and H”.
D and H are “specifiers”
A B C E D F G H
A B C D E F G H
1 2
Tree 1 (A(B(C(D(E(F(G,H))))))) Taxon X = D, E, F, G, H
Tree 2 (A(B(C(E(D(F(G,H))))))) Taxon X = D, F, G, H
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52. I. Progress in phylogenetic reconstruction in Fungi/Agaricomycetes
II. Progress in classification in Agaricomycetes
III. Automated Phylogenetic Taxonomy in Agaricomycetes
IV. Conclusions and future directions
53. Conclusions
Current taxonomic and phylogenetic practices are failing in two key areas:
Integration of available and emerging data
Translation of trees into classifications
Core elements of taxonomy--tree building and translation of trees into classifications--can
be automated. Phylogenetic definitions of taxa (ranked or unranked) are essential for
this purpose.
But, expert user input is still required for:
Curation of the backbone tree
Clade definition
mor does not replace traditional taxonomy, produce monographs or keys
mor is most useful for mega-diverse, poorly-known groups, with large quantities of
emerging data, including Fungi and all groups of “microbes”
54. Future directions
Enhancements:
Improvements to alignment and phylogenetic analysis routines
RAxML, Parsimony Ratchet, TNT, DCM…
Incorporation of ITS data, including environmental sequences
Will dramatically expand taxonomic content
May enable sequence-based species discovery
Will require automated supertree analyses
Will require protocols to assign correspondence between ITS and nuc-lsu
sequences
Expansion to all groups of Fungi (AFTOL2)
Definition of many more clades
Integration with traditional taxonomy
Possible because taxonomic hierarchies have an inherent tree structure
Will require protocols for determining correspondence between sequences and
names (difficult when type specimen has not been sequenced)
Will allow automated construction of trees and classifications that approach the
total knowledge of fungal diversity and phylogeny
55. Ryan Twomey*, Pete Stein*
R. Henrik Nilsson
J. P. Burke*
Not shown: Moran Shonfeld*, Mario Fonseca*,
Thomas Heider*, Daniel Menard* Brandon Gaytán* Marc Snyder*
*undergraduates Janine Costanzo*