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4.6
GROWTH IN
MULTICELLUL
AR
ORGANISMS
A series of changes in the life of an organism
–from zygote to adult – involves two major
processes:
(i) Growth
(ii) Development
Growth Phases
Introduction
Any irreversible and permanent increase in
quantitative parameters that can be measured
such as:
Growth Phases
Definition of Growth
size
length
volume
height
number
of cells
dry
masscytoplasmic
mass
surface
area
Any qualitative change and involve cell
differentiation:
Growth Phases
Definition of Development
shape
specificity
complexity
Growth
Involves 3 processes:
• Cell division – mitosis
• Cell elongation
• Cell differentiation
Growth Phases
1. Cell Division
• mitotic cell division in all somatic cells
• Through mitosis, the number of cells increase
exponentially – 1-2-4-8-16-32
Growth Phases
1. Cell Division
Plants – occurs in apical meristem (Root tip/shoot tip)
• Cells small, closely arranged, large nucleus
• Thin cell wall, no vacuole, dense cytoplasm
Growth Phases
2. Cell Elongation
 Plants - cells elongate & expand due to:Plants - cells elongate & expand due to:
-- absorption of water, into the cells by osmosis,, into the cells by osmosis,
- vacuoles increase in size- vacuoles increase in size
- small vacuoles fuse to form a large central vacuole- small vacuoles fuse to form a large central vacuole
Growth Phases
3. Cell Differentiation
• the cell
undergoes change
in shape
 in order to
perform specialized
functions - same set of
genetic information to
produce a diversity of cell
types
• e.g. : meristematic vascular cells
• the meristematic cells differentiate into:
 xylem: transportation of
water and minerals
 phloem: transportation of
products of photosynthesis and
hormones
Growth Phases
3. Cell Differentiation
GROWTH OF CELLS
CELL DIFFERENTIATION
Guard cell
and stomata
Mesophyll
cell
phloem xylem Parencymatous
cell of cortex
Growth Phases
Plants – In Root Tip
Three phases / zones:
1. Cell divisions
2. Cell elongation
3. Cell differentiation
THE GROWTH ZONE IN A PLANT SHOOT
Zone of cell division/
growth zone
Zone of cell
elongation
Zone of cell
differentiation
Matured tissue
Leaf
primordium
xylem
phloem
cambium
Apical meristem
-produces leaf primordium
which protects the
meristematic tissue
Axial bud
-containing cells which are
capable to divide to form
side branches.
1. Cell divisions
2. Cell elongation
3. Cell differentiation
• Surface area of a leave
can be determine by
draw the outline of the
leave at a graph paper
or square paper (1cm x
1cm) and count the
square inside the leave
outline
• What is the surface are
for the leave?
 20 cm2
/ / /
/ / / / / /
/ / / / /
/ / / /
/ /
Measuring Growth
Surface Area
the more appropriate parameters for the
measurement of growth in multicellular
organisms are the weight or mass
Measuring Growth
• fresh mass/weight
• dry mass/weight
• Periodic linear measurementPeriodic linear measurement
Eg: Measuring height for human being –
the best way to measure the growth of an
individual
Measuring Growth
Estimate length/ height
• the mass of the organism in the normalnormal condition
Measuring Growth
Fresh Mass
The advantages
• easier and convenient to measure
• no need to destroy the specimen
• the same organism can be used for repeated
measurement
The disadvantages
• inaccurate and inconsistent
• because it is affected by the fluctuation of
the amount of water in the organism
Measuring Growth
Fresh Mass
( water ~ 70 - 90% wet mass )
• the mass of the organism after its water
content is fully eliminated
Measuring Growth
Dry Mass: Definition
• it implies the actual growthactual growth of the organism
1. The specimen is destroyed…
Measuring Growth
Dry Mass: Methods
2. …then transferred into the oven at 110°C
to eliminate its water content.
3. Next, it is put inside a desiccator to
ensure the water is completely eliminated.
4. The dried specimen is weighed.
5. The above procedures are repeated until
a constant value is obtained.
Measuring Growth
Dry Mass
The advantages
• more accurate
• the amount of water in an organism is not
constant, i.e. depends on the water intake
by the individual organism
Measuring Growth
Dry Mass
The disadvantages
• destruction of specimens
• in repeated measurement, specimens of the
similar age, size are required (destroyed!) to
avoid or minimize errors in the measurement
• Large samples of genetically identical organism
need to be grown under similar condition
• Time consuming
If an organism’s measurements (height,
weight, etc) are plotted against time, a
growth curve is obtained.
GROWTH
CURVE
Sigmoid Curve
Maturity
phase
Senescence
& death
GROWTH STAGES
1. Lag phase:
(a)The beginning of the growth process
(b)The rate of growth is generally low
(c)Cell division by mitosis takes place
very actively but there is no increase in cell size.
2. Exponential Growth:
a) The highest growth rate
b) Increase in the number of cells by mitosis
c) Cell size increases by absorbing water and addition of
protoplasmic substances.
3. Maturity Stage
a) Growth rate is low
b) Cells achieve the optimum size(adult)
and there is no more increase in size of
the cell.
a) A little cell division to replace damaged cells
4. Stationary (Adult) Stage
a) Growth rate is zero
b) Cells still divide to replace damaged / dead cells
5. Senescence Stage
a) Growth rate is negative because of loss of tissues due to aging
process
HUMANS GROWTH CURVE
VIDEO
GROWTH CURVE OF A HUMAN
GROWTH CURVE OF A HUMAN
Time / year
Growth ( height/
weight)
At birth
Rapid growth
Slow growth
Very rapid growth
Very little
growth death
senescence
adult /Adolescence /
childhood
baby
GROWTH OF A BOY AND A GIRL
Rate of increase in height Kadar pertambahan berat
Age /
years
Height/cm
boy
girl
Freshweight
Rate of increase in weight
boy
girl
Age /
years
GROWTH CURVE OF
INSECTS
VIDEO
Grasshoppers moult (shed skin) 5 times from 1st
instar to adult
Growth curve of insects
– staircase shape
PRIMARY &
SECONDARY
GROWTH IN PLANTS
VIDEO
Growth in Plant
Meristem
Actively dividing tissue.
• Apical meristem: These are found at the
tips of roots and shoots and are
responsible for primary growth of the
plant. They increase its length.
• Lateral meristem:
• Cambium & Cork Cambium
• They are responsible for secondary
growth and cause an increase in girth
Primary & Secondary Growth
• All plants exhibit primary growth
• Woody plants also undergo secondary
growth (increase in girth wood)
• Herbaceous plants do not undergo
secondary growth
Primary Growth
• Primary growth leads to an increase in length
of the plant shoots or roots.
• It is due to the activity of apical meristems.
http://www.ucalgary.ca/~biolcore/biol233/plant5/apicalm
agon.seowon.ac.kr/~bioedu/bio/ohp/t-188.jpg
Root apical meristem (RAM)
Shoot apical meristem (SAM)
THE GROWTH ZONE IN A PLANT SHOOT
Zone of cell division/
growth zone
Zone of cell
elongation
Zone of cell
differentiation
Matured tissue
Leaf
primordium
xylem
phloem
cambium
Apical meristem
-produces leaf primordium
which protects the
meristematic tissue
Axial bud
-containing cells which are
capable to divide to form
side branches.
THE GROWTH ZONE IN A PLANT ROOT
Root hair
phloem
xylem
Matured tissue
Zone of cell division/
growth zone
Zone of cell
differentiation
Zone of cell elongation
cortex
Apical
Meristem
Root
cap
SECONDARY GROWTH IN
PLANTS
Secondary growth leads to the increase in thickness of a
plant. This is due to lateral meristems. (dicots only)
(perennial plants only)
Structure of Dicot Stem
Lateral meristem – vascular cambium & cork cambium
.Vascular cambium divides to form cambium ring.
.Cambium ring divides tangentially to form secondary xylem inside & secondary
hloem outside.
.Cork cambium divides to form cork outside.
.Lenticels develop to allow gaseous exchange between air & stem
.Primary xylem, phloem and pith are “squashed”. Epidermis ruptures.
Secondary Growth
Xylem
year 1
year 2
year 3
PithVascular
Cambium
Phloem
Cork
Cork Cambium
Note:
Xylem = wood
Cork, Cork cambium and
Phloem = bark
http://www.umanitoba.ca/faculties/science/biological_sciences/lab9/images/til
• Each year a new ring of xylem – produces a hard inner core
called wood
• Spring - fast growth – cells large, thin wall
• Autumn - slow growth – cells small , thick walls
• Light & dark rings – annual rings – calculate age of tree
Structure of Dicotyledonous Root
dicotyledonous root
Lateral meristem – vascular cambium & cork cambium
1.Vascular cambium divides to form cambium ring.
2.Cambium ring divides tangentially to form secondary xylem inside & secondary
phloem outside.
3.Cork cambium divides to form cork outside.
4.Primary xylem & phloem compressed.
THE END
Chapter review…Chapter review…
Growth
Growth phases
Growth curveGrowth curve
sigmoid human Secondary growth
(plants)
insects
Primary growth
(plants)
Growth
measurement
Wet/fresh
mass
Dry
mass
Definition
Phases of growth
Cell division
Cell enlargement
Cell differentiation
Growth in Multicellular Organisms
Growth in Multicellular Organisms

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Growth in Multicellular Organisms

  • 2. A series of changes in the life of an organism –from zygote to adult – involves two major processes: (i) Growth (ii) Development Growth Phases Introduction
  • 3. Any irreversible and permanent increase in quantitative parameters that can be measured such as: Growth Phases Definition of Growth size length volume height number of cells dry masscytoplasmic mass surface area
  • 4. Any qualitative change and involve cell differentiation: Growth Phases Definition of Development shape specificity complexity
  • 5. Growth Involves 3 processes: • Cell division – mitosis • Cell elongation • Cell differentiation
  • 6. Growth Phases 1. Cell Division • mitotic cell division in all somatic cells • Through mitosis, the number of cells increase exponentially – 1-2-4-8-16-32
  • 7. Growth Phases 1. Cell Division Plants – occurs in apical meristem (Root tip/shoot tip) • Cells small, closely arranged, large nucleus • Thin cell wall, no vacuole, dense cytoplasm
  • 8. Growth Phases 2. Cell Elongation  Plants - cells elongate & expand due to:Plants - cells elongate & expand due to: -- absorption of water, into the cells by osmosis,, into the cells by osmosis, - vacuoles increase in size- vacuoles increase in size - small vacuoles fuse to form a large central vacuole- small vacuoles fuse to form a large central vacuole
  • 9. Growth Phases 3. Cell Differentiation • the cell undergoes change in shape  in order to perform specialized functions - same set of genetic information to produce a diversity of cell types
  • 10. • e.g. : meristematic vascular cells • the meristematic cells differentiate into:  xylem: transportation of water and minerals  phloem: transportation of products of photosynthesis and hormones Growth Phases 3. Cell Differentiation
  • 11. GROWTH OF CELLS CELL DIFFERENTIATION Guard cell and stomata Mesophyll cell phloem xylem Parencymatous cell of cortex
  • 12. Growth Phases Plants – In Root Tip Three phases / zones: 1. Cell divisions 2. Cell elongation 3. Cell differentiation
  • 13. THE GROWTH ZONE IN A PLANT SHOOT Zone of cell division/ growth zone Zone of cell elongation Zone of cell differentiation Matured tissue Leaf primordium xylem phloem cambium Apical meristem -produces leaf primordium which protects the meristematic tissue Axial bud -containing cells which are capable to divide to form side branches. 1. Cell divisions 2. Cell elongation 3. Cell differentiation
  • 14.
  • 15.
  • 16.
  • 17. • Surface area of a leave can be determine by draw the outline of the leave at a graph paper or square paper (1cm x 1cm) and count the square inside the leave outline • What is the surface are for the leave?  20 cm2 / / / / / / / / / / / / / / / / / / / / Measuring Growth Surface Area
  • 18. the more appropriate parameters for the measurement of growth in multicellular organisms are the weight or mass Measuring Growth • fresh mass/weight • dry mass/weight
  • 19. • Periodic linear measurementPeriodic linear measurement Eg: Measuring height for human being – the best way to measure the growth of an individual Measuring Growth Estimate length/ height
  • 20. • the mass of the organism in the normalnormal condition Measuring Growth Fresh Mass The advantages • easier and convenient to measure • no need to destroy the specimen • the same organism can be used for repeated measurement
  • 21. The disadvantages • inaccurate and inconsistent • because it is affected by the fluctuation of the amount of water in the organism Measuring Growth Fresh Mass ( water ~ 70 - 90% wet mass )
  • 22. • the mass of the organism after its water content is fully eliminated Measuring Growth Dry Mass: Definition • it implies the actual growthactual growth of the organism
  • 23. 1. The specimen is destroyed… Measuring Growth Dry Mass: Methods 2. …then transferred into the oven at 110°C to eliminate its water content. 3. Next, it is put inside a desiccator to ensure the water is completely eliminated. 4. The dried specimen is weighed. 5. The above procedures are repeated until a constant value is obtained.
  • 24. Measuring Growth Dry Mass The advantages • more accurate • the amount of water in an organism is not constant, i.e. depends on the water intake by the individual organism
  • 25. Measuring Growth Dry Mass The disadvantages • destruction of specimens • in repeated measurement, specimens of the similar age, size are required (destroyed!) to avoid or minimize errors in the measurement • Large samples of genetically identical organism need to be grown under similar condition • Time consuming
  • 26. If an organism’s measurements (height, weight, etc) are plotted against time, a growth curve is obtained. GROWTH CURVE
  • 28. GROWTH STAGES 1. Lag phase: (a)The beginning of the growth process (b)The rate of growth is generally low (c)Cell division by mitosis takes place very actively but there is no increase in cell size. 2. Exponential Growth: a) The highest growth rate b) Increase in the number of cells by mitosis c) Cell size increases by absorbing water and addition of protoplasmic substances.
  • 29. 3. Maturity Stage a) Growth rate is low b) Cells achieve the optimum size(adult) and there is no more increase in size of the cell. a) A little cell division to replace damaged cells 4. Stationary (Adult) Stage a) Growth rate is zero b) Cells still divide to replace damaged / dead cells 5. Senescence Stage a) Growth rate is negative because of loss of tissues due to aging process
  • 31.
  • 32. GROWTH CURVE OF A HUMAN
  • 33. GROWTH CURVE OF A HUMAN Time / year Growth ( height/ weight) At birth Rapid growth Slow growth Very rapid growth Very little growth death senescence adult /Adolescence / childhood baby
  • 34. GROWTH OF A BOY AND A GIRL Rate of increase in height Kadar pertambahan berat Age / years Height/cm boy girl Freshweight Rate of increase in weight boy girl Age / years
  • 36.
  • 37. Grasshoppers moult (shed skin) 5 times from 1st instar to adult
  • 38. Growth curve of insects – staircase shape
  • 39.
  • 41. VIDEO
  • 43. Meristem Actively dividing tissue. • Apical meristem: These are found at the tips of roots and shoots and are responsible for primary growth of the plant. They increase its length. • Lateral meristem: • Cambium & Cork Cambium • They are responsible for secondary growth and cause an increase in girth
  • 44. Primary & Secondary Growth • All plants exhibit primary growth • Woody plants also undergo secondary growth (increase in girth wood) • Herbaceous plants do not undergo secondary growth
  • 45. Primary Growth • Primary growth leads to an increase in length of the plant shoots or roots. • It is due to the activity of apical meristems. http://www.ucalgary.ca/~biolcore/biol233/plant5/apicalm agon.seowon.ac.kr/~bioedu/bio/ohp/t-188.jpg Root apical meristem (RAM) Shoot apical meristem (SAM)
  • 46. THE GROWTH ZONE IN A PLANT SHOOT Zone of cell division/ growth zone Zone of cell elongation Zone of cell differentiation Matured tissue Leaf primordium xylem phloem cambium Apical meristem -produces leaf primordium which protects the meristematic tissue Axial bud -containing cells which are capable to divide to form side branches.
  • 47. THE GROWTH ZONE IN A PLANT ROOT Root hair phloem xylem Matured tissue Zone of cell division/ growth zone Zone of cell differentiation Zone of cell elongation cortex Apical Meristem Root cap
  • 48. SECONDARY GROWTH IN PLANTS Secondary growth leads to the increase in thickness of a plant. This is due to lateral meristems. (dicots only) (perennial plants only)
  • 50. Lateral meristem – vascular cambium & cork cambium .Vascular cambium divides to form cambium ring. .Cambium ring divides tangentially to form secondary xylem inside & secondary hloem outside. .Cork cambium divides to form cork outside. .Lenticels develop to allow gaseous exchange between air & stem .Primary xylem, phloem and pith are “squashed”. Epidermis ruptures.
  • 51.
  • 52.
  • 53. Secondary Growth Xylem year 1 year 2 year 3 PithVascular Cambium Phloem Cork Cork Cambium Note: Xylem = wood Cork, Cork cambium and Phloem = bark http://www.umanitoba.ca/faculties/science/biological_sciences/lab9/images/til • Each year a new ring of xylem – produces a hard inner core called wood • Spring - fast growth – cells large, thin wall • Autumn - slow growth – cells small , thick walls • Light & dark rings – annual rings – calculate age of tree
  • 55. dicotyledonous root Lateral meristem – vascular cambium & cork cambium 1.Vascular cambium divides to form cambium ring. 2.Cambium ring divides tangentially to form secondary xylem inside & secondary phloem outside. 3.Cork cambium divides to form cork outside. 4.Primary xylem & phloem compressed.
  • 56.
  • 58. Chapter review…Chapter review… Growth Growth phases Growth curveGrowth curve sigmoid human Secondary growth (plants) insects Primary growth (plants) Growth measurement Wet/fresh mass Dry mass Definition Phases of growth Cell division Cell enlargement Cell differentiation