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Biochem recombinant dna technology(29.6.10)
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Viruses are infectious intracellular obligate parasites with subcellular level of organisation and without protoplasm, cell, cell organells and the molecular machineries for energy metabolism and protein synthesis,Grouped on the basis of size and shape, chemical composition and structure of the genome and mode of replication most viruses have a specific shape that is determined by the capsomeres or the envelope. Capsid symmetry - Three types (1) HELICAL CAPSID (2) ICOSAHEDRALCAPSID (3) COMPLEX CAPSID Capsid - large macromolecular structures. Made up of proteins called capsomers. Chemical unit of capsomers are polypeptide chain. Capsid - surrounded by lipoprotein layer called envelop Envelop is made up of proteins and glycoproteins Presence of lipid -envelope seems flexible and loose. Envelope is composed of both the host viral components. projections on the envelope known as spikes/peplomers which are arranged into distinct units.
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A recombinant DNA molecule is produced by joining together two or more DNA segments usually originating from two different organisms. More Specifically, a recombinant DNA molecule is a vector into which desired DNA fragment has been inserted to enable its cloning in an appropriate host. Recombinant DNA molecules are produced with one of the following objectives: 1. To obtain large number of copies of specific DNA fragments. 2. Large scale production of the protein encoded by the gene. 3. Integration of the desired DNA fragment into target organism where it expresses itself. Drought tolerant-genetically modified plants: Present abiotic stress is a major challenge in our quest for sustainable food production as these may reduce the potential yields by 70% in crop plants Of all abiotic stress, drought is regarded as the most damaging Transgenic plants carrying genes for abiotic stress tolerance are being developed for water stress management Conventional breeding approaches, involving inter specific and inter generic hybridizations and mutagenesis have been limited success. Major problems have been the complexity of drought tolerance & low genetic yield components under drought conditions. Unlike conventional plant breeding there is no need of repeated back crossing Gene pyramiding or gene stacking through co-transformation of different genes with similar effects can be achieved.
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