In this vendor presentation given at WCBP, we introduce a new glycan fluorescent label, RapiFluor-MS, which is used to label N-linked glycans. This innovative label improves FLR and mass spectrometry signals for glycan characterization and profiling analysis. Plus - our GlycoWorks RapiFluor-MS N-Glycan Kit now allows you to finish glycan deglycosylation, labeling and cleanup in 3 steps and just 30 minutes.
Learn more about this novel technology and its applications for glycosylated proteins:
http://www.waters.com/glycans
See more of Waters' solutions for biopharmaceutical laboratories:
http://www.waters.com/biopharmaceutical
Playback a full video of this talk, recorded Jan. 27, 2015 at WCBP:
http://www.waters.com/waters/library.htm?cid=10116552&lid=134833758
Based on the observed chromatographic peak areas, response factors for fluorescence and MS detection were determined.
These results indicate that RapiFluor-MS labeled glycans produce 2x higher fluorescence signal and, more astoundingly, nearly 800x greater MS signal than N-glycans labeled with Instant AB.
In this slide, deconvoluted mass spectra are presented for a mAb subjected to various conditions.
The top spectrum shows the mAb before any treatment.
The middle spectrum after treatment with only deglycosylation conditions.
Lastly, the bottom spectrum after our combined approach of surfactant-based heat denaturation and Rapid PNGase F deglycosyaltion.