The Effect of Electrical Stimulation on Adipose Stem Cells Cultured in Conductive Stereolithographic Scaffold Structures by Suvi Haimi

1. The Effect of Electrical Stimulation
on Adipose Stem Cells Cultured in
Conductive Stereolithographic
Scaffold Structures
Suvi Haimi, PhD
Adult Stem Cell Group, BioMediTech,
University of Tampere, Finland
&
Department of Biomaterials Science and Technology,
University of Twente, The Netherlands

2. BioMediTech
• Tissue engineering and stem cell
technology
• Biomaterials
• Sensor and actuator technologies
• Measurement and imaging technologies
• Biotechnology
• Immunology
• Cancer and mitochondrial research
• Systems biology
• Bioinformatics
• Computational methods in biomedicine
BioMediTech is a joint institute of
University of Tampere & Tampere University of Technology
Over 250 scientists in world-class basic and translational research
Tampere, Finland

3. Adult Stem Cell Group:
world leader in stem
cell based
reconstructions of
cranial bones
Example of Research Excellence
Combining stem
cells with
biomaterial
Isolation of
stem cells
 GMP culture for
2-6 weeks
Transplantation
back into same
patient
Combination of
biomaterials and stem
cells differentiated from
subcutaneous fat
Harvesting
of adipose tissue

4. Department of Biomaterials Science and
Technology
University of Twente
Enschede,
The Netherlands
Stereolithography
• Polymer synthesis
• Polymer engineering
• State-of-the-art scaffold processing
techniques

5. Mechanical
stress
CompressionStretch
Fluidflow
+
-
Electrical stimulation (ES) in skeletal tissue
engineering
Wound
healing
Embryonic
development
Action
potential

6. • Used in numerous biomedical applications
• Bioactive – can be easily incorporated with
negatively charged biomolecules
Electrically conductive polypyrrole (PPy)
J Biomed Mater Res A, 2009
Langmuir, 2013
Tissue Eng Part A, 2013
Ann Biomed Eng, 2014

7. Biocompatibility of PPy in vitro
Our team was the first to show
• Biocompatibility with ASCs
• Attachment and spreading can be
enhanced by charging
J Biomed Mater Res A, 2009
Langmuir, 2013
Charged PPy (+)
Non-Charged PPy (0)
100 µm
100 µm

8. Biocompatibility of PPy in vitro
My team was the first to show
• Biocompatibility with ASCs
• Attachment and spreading can be
enhanced by charging
J Biomed Mater Res A, 2009
Langmuir, 2013
Charged PPy (+)
Uncharged PPy (0)
100 µm
100 µm
• Charging increased cell surface area of ASCs at 3h

9. PPy promotes smooth muscle differentiation
Björninen et al. submitted
Calponin/DAPI MHC/DAPI SMA/DAPI

10. Aim
Differentiate hASCs towards skeletal tissues
applying ES via designed poly (trimethylene
carbonate) (PTMC) scaffolds coated with PPy

11. Study design
Electroactive
polypyrrole coating
3D electrical
stimulation
Designed
3D scaffolds
Adipose
stem cells

12. 0
1
2
3
4
5
6
7
8
9
Control ES 7d - rest 7d ES 4h/d ES 8h/d
α-SMA
Calponin
MHC
Relativeexpressionat14d
/Electrical stimulation in 2D- Effects on smooth muscle
differentiation
*
*

13. 0.00
0.50
1.00
1.50
2.00
2.50
3.00
control ES 4h/d ES 8h/d
α-SMA
SM22α
MHC
Relativeexpressionat14d
Electrical stimulation in 3D-Effects on smooth muscle
differentiation

14. Electrical stimulation in 3D enhanced ASC osteogenic
differentiation
*
*
14d
Control
ES
14d

15. Summary
• PPy-coated scaffolds are potential for skeletal
tissue engineering
• ES is potential method to engineer muscle and
bone tissue
• Screening of more effective ES parameters in 3D
environment needed

16. 11.12.2008
Acknowledgements
TAMPERE UNIVERSITY OF TECHNOLOGY
Funding
• Academy of Finland
• TEKES, Finnish Funding Agency for
Technology and Innovation
• Pirkanmaa Hospital District
Competitive research funding, EVO
• The City of Tampere
Adult Stem Cell GroupDepartment of Biomaterials Science and Technology
Key collaborators
•
• Tampere University of Technology,
Finland
• VTT Technical Research Center,
Finland

17. Kiitos / Thank You!