Targeted Drug Delivery System Resealed Erythrocytes discusses using red blood cells (erythrocytes) as carriers for drug and enzyme delivery. Erythrocytes can be isolated from blood and loaded with drugs or enzymes using various methods like hypotonic lysis and resealing. Loaded erythrocytes can then be used to treat conditions by targeting delivery and increasing drug circulation time. New systems like nanoerythrosomes use extruded erythrocyte ghosts to produce smaller drug carrying vesicles.

1. Targeted Drug Delivery System Resealed Erythrocytes

7. Red Blood Cells (Erythrocytes, RBCs) Two million new RBCs are formed every second Diameter of RBC is 6-9 μ Composed of ~90% hemoglobin Each RBC contains ~270 million hemoglobin molecules RBCs have a life-span of ~120 days before they are removed by the spleen Typically 4-8 x 106 cells per ml in normal human blood 6-9 µ

8. Basic features of erythrocytes : Composition of erythrocytes :

16. Electro encapsulation Drug loading in Resealed erythrocytes Membrane perturbation Hypo-osmotic lysis Lipid fusion endocytosis Osmotic-lysis Preswell method Dilution method

24. Time consuming, impermeable to large molecules Better in vivo surveillance --- Isotonic osmotic lysis --- Good retention of cytoplasm 20-70% Preswell dilution Less Entrapment efficiency Fastest and simplest for low mol. Wt. 1-8% Dilution method Disadvantages Advantages %Loading Method

29. In-vitro characterization

33. Pentamidine, Mycotoxine, HOmidium bromide Targeting to RES Albumin, Salbutamol, Tyrosine kinase circulating carriers Human recombinant interleukin-2 Immuno therapy Brinase, Aspirine, Heparine Thrombolytic activity ß-galactosidase, ß-fructo-furoodase, urease Enzyme deficiency, replacement therapy Drug / Enzyme / Macromolecule Application