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Microbial diversity and ecology: Microbial evolution
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Microbial diversity and ecology: Microbial evolution
1.
LS2026 Microbiology 微生物學 陳俊堯
老師 生命科學系 D928 cychen@mail.tcu.edu.tw
2.
Chemoheterotroph Chemolithotroph
Photoheterotroph Photolithotroph 2
3.
根據 DNA
相似程度區分 Prokaryotes Archaea 古菌界 Bacteria 細菌界 Eucarya 真核界 3
4.
Eukaryote =
archaea + 產能的 bacteria (mitochondria) + 利用光的 cyanobacteria (cyanobacteria) 4 http://learn.genetics.utah.edu/content/cells/organell
5.
外型很相似,不能像動植 物那樣用外型區分。 先來看看傳統方法,再來 看現代方法。 5 blog.qatestlab.com
6.
形態:細胞是球菌或桿菌, 大小,
菌落顏色, 形狀等。 生理活性:能不能利用 glucose, 需不需要氧氣, 會 不會還原 nitrate, 能不能氧化鐵。 缺點:會受到趨同演化的影響 6 classroom.sdmesa.ed
7.
利用 DNA
序列來推算演化關係,當做分類的依據。 7 betastuffs.blogspot.com
8.
所有微生物都有的基因 演化速度不能太快
突變累積速度維持一致 8 威 蝦 米 哩
9.
rRNA 是組成
ribosome 的零件之一 Ribosome 有 small 和 large subunits 9 serc.carleton.edu
10.
1 http://www.insectscience.org/10.159/ 原核:16S rRNA
gene 真核:18S rRNA gene 合稱 small subunit RNA (ssRNA) 人氣 基因
11.
所有微生物都有的基因 演化速度不能太快
突變累積速度維持一致 1 有 沒 有 哩
12.
誰需要鑑定細菌? 病原菌鑑定
環境微生物鑑定 食品微生物鑑定 1 www.healthcarerelocationservices.com
13.
以 PCR
放大 16S rRNA 基因序列 進行 DNA 定序 對得到的序列跟資料庫 進行比對 1
14.
利用傳統鑑定方法當做 確認的工具。 1
15.
1 Deep-branching lineage Bacteria
Archaea Eucarya
16.
Euryarchaeota Crenarchaeota Korarchaeota Nanoarchaeota Thaumarchaeota
17.
Deep- branching lineages Gram
positive bacteria Gram negative bacteria
18.
Deinococcus Chloroflexi
Actinobacteria Firmicutes Cyanobacteria Bacteroidetes Spirochaetes Proteobacteria
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