Asian American Pacific Islander Month DDSD 2024.pptx
Microscope Biology I Lab
1. Microscope
• Micro (small)
• Scope (view)
Three common types:
1. Compound Light
2. Stereo
3. Electron (2 versions)
2. 1. Compound Light
• Most common
• 2 lenses focusing at the same time
1. Ocular
2. Objective
• 2D image
• High magnification through light
absorption
4. Term: Magnification
• Ratio of image size to actual size
• Ocular: 10X
(“X” means times; for example, the ocular
magnifies something 10 times)
• 4 objectives
– Scan: 4X
– Low Power: 10X
– High Power: 40X
– Oil immersion: 100x
• Multiply ocular x objective to get
overall magnification
5. Field of View
Amount of object you can see
• Decreases with increasing magnification
6. Depth of Focus
Thickness of specimen in focus at a given
magnification
• Decreases with increasing magnification
• Lab says to make your own slide-we will use a
prepared thread slide
7. Inversion Phenomenon
• Objects appear upside down and
backwards in the compound microscope
• We will use prepared letter e slides
8. 2. Stereomicroscope
• Used to get a better look at larger objects
• 3D image
• Specimen not mounted on a slide
• Low magnification
• Uses visible light through light scattering
9. 3. Electron Microscope
• Uses electron streams focused by
magnets to view specimens, not light
• 2 types
1. Scanning
2. Transmission
12. Wet mount
• Way to create a simple slide
• Need a clean slide and a coverslip
• Procedure
– Place specimen on slide
– Add a drop of water
– Place coverslip over specimen at an angle to avoid air
bubbles
– Wipe away excess water
– View under microscope
• Today: pond water wet mount
Notas do Editor
Image of phase contrast microscope by GcG
https://commons.wikimedia.org/wiki/File:Phase_contrast_microscope.jpg
Public Domain
Image of parts of the microscope by Peri Coleman
https://commons.wikimedia.org/wiki/File:Labelledmicroscope.gif
Public Domain
Image of microscope lens by Halfblue
https://commons.wikimedia.org/wiki/File:Eyepieces_random_selection.jpg
CC-By SA
Image of optic lenses on microscope by Rama
https://commons.wikimedia.org/wiki/File:Loupe-binoculaire-p1030891.jpg
CC-By SA
Image of microscope lenses by PublicDomainPictures
https://pixabay.com/en/microscope-slide-research-close-up-275984/
Public Domain
Image of Optical stereo microscope nikon smz10 by GcG(jawp)
https://commons.wikimedia.org/wiki/File:Optical_stereo_microscope_nikon_smz10.jpg
Public Domain
Image of Scanning Electron Microscope by Cjp24
https://commons.wikimedia.org/wiki/File:Scanning_electron_microscope2.jpg
CC-By SA
Image of pollen by Dartmouth College Electron Microscope Facility
https://commons.wikimedia.org/wiki/File:Misc_pollen.jpg
Public Domain
Image of mitochondria, mammalian lung – TEM by Louisa Howard
https://commons.wikimedia.org/wiki/File:Mitochondria,_mammalian_lung_-_TEM.jpg
Public Domain
Image of Transmission electron microscope (Morgangni 268D) 1pl by Pleple2000
https://commons.wikimedia.org/wiki/File:Transmission_electron_microscope_(Morgagni_268D)_1pl.jpg
CC-By SA
Image of Bdelloid Rotifer by Bob Blaylock
https://commons.wikimedia.org/wiki/File:Bdelloid_Rotifer.jpg
CC-By SA