7. Chemical analysis Sophisticated tools are available for studying the structural composition of bacteria (most commonly fatty acid , carbohydrate or ubiquinone profiling) of bacteria. Characterization of secreted metabolic products (e.g. volatile alcohols and short chain fatty acids ) is also helpful. Dr.M.K. SATEESH
8.
9. A model for bacterial speciation Dr.M.K. SATEESH
21. Protein profiles/fingerprints Total Cellular membrane proteins PAGE/ Isoelectric focusing ~ 10-30 proteins ( water soluble) Clostridium, Rhizobium, Haemophilus Dr.M.K. SATEESH
22. Enzyme characterization Citrate synthase is found in all living cells, so it has been a useful enzyme for comparing differences from organism to organism. Citrate synthase Dr.M.K. SATEESH
24. Succinyl-CoA synthetase, ( succinate thiokinase) succinyl-CoA synthetase, sometimes called succinate thiokinase Dr.M.K. SATEESH
25.
26. Oxidation - Reduction Reactions are Always Coupled Oxidation = A B H + H { { { Reduction = gain of e - & H + loss of e - & H + NAD + NADH + H = electron Dr.M.K. SATEESH B A
27. MKS Use of Intact Cell MALDI Fingerprints to Identify Bacillus and Pseudomonas Isolates