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DIATOMS
CHARACTERISTICS
   INTRODUCING ME…
Characteristic # 1

 Diatoms are…

                                        the most abundant
                                      species in AQUATIC/
                                         moist ecosystems



http://www.jm-derochette.be/images/Diatoms/Diatoms_1.jpg
Characteristic # 2

 Diatoms are…


                               Azpeitia tabularis




    Important:                                            Photosynthetic
    • Fucoxanthin
                                                             autotrophs
http://www.sciencephoto.com/media/16036/enlarge,
http://berryberryeasy.com/wp-content/uploads/2011/06/Photoautotrophs.jpg
Characteristic # 3

 Diatoms are…




  Psammodictyon panduriforme                                     UNICELLULAR
                                                                PROTISTS/ alga/
                                                                  phytoplankton
http://1.bp.blogspot.com/_C97NrIblNPQ/TTkPj0DIgZI/AAAAAAAAB38/QYLupyEthaw/s640/Diatom007.jpg,
http://www.cartage.org.lb/en/themes/sciences/botanicalsciences/majordivisions/kingdomprotista/Protists/98258d
.jpg
Characteristic # 4

 Diatoms are…
                                              Triceratium sp.




           Biddulphia tridens


                             The most beautiful creatures
                                                                 on earth
http://www.sciencephoto.com/image/15909/350wm/B3050211-Diatom_alga,_SEM-SPL.jpg,
http://cdn.indulgy.com/78/b3/Q8/210684088788027965687KsAPSc.jpg
STRUCTURAL
 CHARACTERISTICS
THAT’S WHAT MAKES THEM BEAUTIFUL!
Structural Characteristic # 1

 Diatoms have…
                                               Functions:
                                               • Filtration/ Disinfection
                                               • Industry (eg. toothpastes,
                             Epitheca          soap)
                                               • Gives them shape (boat-
                                               like, rod-like, disk-like,
                              Hypotheca        triangle-like)
                                               • Gives them color

    Stichodiscus sp.
                                              Glass-like Cell
                                          walls(Frustule)with
                                                 silica (siO2)
http://www.sciencephoto.com/image/15918/530wm/B3050220-
Diatom_alga,_SEM-SPL.jpg
Structural Characteristic # 1




http://www.ucl.ac.uk/GeolSci/micropal/images/diat/diadiag01.gif
Structural Characteristic # 2

 Diatoms have…
                                                  Functions:
                                                  • Photosynthesis
                                                      • Primary Food Source
                                                      • Vitamin D Source




                                   Chlorophyll-like plastids
                                          inside their cells


http://www.chm.bris.ac.uk/motm/chlorophyll/chphyll.gif
Structural Characteristic # 3

Diatoms have…
Functions:
• Oil production
     • Floating
     • Oil Deposits under the
     ocean      fuel                      Oil drops made of
     • Storage of chemical energy
     from photosynthesis
                                        sugar & starch and
• Nitrogen production
                                    volutin inside their cells
Structural Characteristic # 4

 Diatoms have…
                                                    Functions:
                                                    • Oxygen and Nitrogen
                                                    Distribution
                                                    • Exchange of materials




                                                      Pores with
                                                patterns on their
                                                        frustules
http://1.bp.blogspot.com/_C97NrIblNPQ/TTkbPZID0nI/AAAAAAAAB4s/cyjz8FLyF_g/s640/
rad_diatoms.jpg
Structural Characteristic # 4

 Diatoms have…
                                               Effects:
                                               • Immobility




                                                   NO cilia and
                                                       flagella
           Aulacodiscus oregonus

http://www.sciencephoto.com/image/15915/530wm/B3050217-
Diatom_alga,_SEM-SPL.jpg
Structural Characteristic # 5

 Diatoms have…



                                                  Nitzschia reinholdii

                                                     Different sizes
                                      Types:
   Actinocylcus sp.                   • Centric         and shapes
                                      • Pennate

http://2.bp.blogspot.com/_C97NrIblNPQ/TTkiNLuvrEI/AAAAAAAAB5c/9KwK--
vMrG8/s400/Diatom-2.jpg, http://www.sciencephoto.com/image/15921/530wm/B3050223-
HABITAT
SOMEWHERE ONLY THEY KNOW
Habitats

 Diatoms live in…




http://www.allabouthappylife.com/wallpaper/widescreen_wallpapers/blue_water/blue_ocea
n-dsc05506.jpg, https://cmsdata.iucn.org/img/freshwater_6352.jpg
Habitats
                                                        Habitats

 Diatoms live in…




                                                      NO cilia and
                                                          flagella

http://www.scienceclarified.com/images/uesc_04_img0211.jpg
Habitats




http://cornellbiochem.wikispaces.com/file/view/Picture_1.png/139679067/301x313/
Picture_1.png
NUTRITION
MAKULAY ANG BUHAY SA SINABAWANG
             GULAY!
Nutrition

 Diatoms are…




http://www.youtube.com/watch?v=__xEh1FrhLM,
http://mrhartansscienceclass.pbworks.com/f/1328587105/10-20-PhotosynthesisRev-L.gif
REPRODUCTION
 I JUST HAD… (BLEEP)!
Asexual Reproduction

The shrinking phase
1. Cell division takes place.
2. The two valves (epitheca and hypotheca) separate.
3. Each valve forms an epivalve of the daughter cell while
   hypovalves are secreted from the parent cells.
4. This results to one cell being the same size as the parent
   cell while the other is smaller.
5. This process continues until cells reach about 30%-40%
   of their original size.
Asexual Reproduction




http://www.mbari.org/staff/conn/botany/phytoplankton/images/diatom_asexual.j
pg
Sexual Reproduction

The growth phase
1. Meiotic Cell division
   • Formation of eggs and monoflagellated sperms in
      the parent cell
2. Fertilization
   • Union of egg and sperm produces auxospore
3. Auxospore/Zygote Formation (Growth Phase)
   • Splitting of the parent cell while continuing to grow
4. Test Secretion
   • End of growth phase; new parent cell develops
Sexual Reproduction




http://rbg-
web2.rbge.org.uk/algae/auxospores/auxospore_files/cycle_reproduction.jpg
Reproductive Cycle




http://upload.wikimedia.org/wikipedia/commons/thumb/8/8a/Centric_diatom_lif
e-cycle.jpg/580px-Centric_diatom_life-cycle.jpg
Resting Spores

 The inactivity phase
      Skeletonema marinoi
                                      When: Times of low nutrients
                                        and low sunlight
                                      What: Metabolically inactive
                                        spores
                                      How: Stored energy from
                                        photosynthetic products &
                                        thick and tough cell walls
                                      Where: At the bottom of the
                                        ocean

http://images.sciencedaily.com/2011/03/110301111658-large.jpg
ECOLOGY
I WAS BORN THIS WAY!
Ecological Role # 1

 Primary producers




http://oceansjsu.com/images/exped_ecoystems/arctic_marine_food_web_90.jpg
Ecological Role # 2

Important resource
•   20%-25% of the world’s carbon fixation
•   40% of the world’s oxygen (in the atmosphere)
•   Vitamin D
•   23% of primary productivity in the world
•   Oil/Fuel
•   Silicon (SiO2)
Ecological Role # 3

 Nutrient detector




 PLANKTON BLOOM – when there is an abundance of
    nutrients and excessive amount of sunlight in the ocean
http://oceansjsu.com/images/exer8/plankt1.gif
EXAMPLES
I’M NEVER CHANGING WHO I AM!
Example # 1

 Triceratium moreirae
 Where: (mentioned)
 Southern Brazil
 Who: L.F. Fernandes and
 R.M. Sousa-Nosimann
 When: 2001
 What: 47-61 μm; convex,
 tripolar valves; elevated
 with ocellus and
 rimoportula
http://www.scielo.br/img/fbpe/rbbio/v61n1/a20fig6b.gif
Example # 2

 Lithodesmium undulatum
 Where: warm, temperate
 seas
 Who: C.G. Ehrenberg
 When: 1839
 What: 37-93 μm; marginal
 ridge with clear pattern of
 perforation; undulated
 sides; ‘wavy’

http://www.sciencephoto.com/image/15919/530wm/B3050221-
Diatom_alga,_SEM-SPL.jpg
Example # 3

 Baceteriastrum delicatulum
 Where: (mentioned)
 Mexico, Lebanon, UK,
 Red Sea
 Who: Cleve
 When: 1897
 What: 20-40 μm; circular
 valves with bristles;
 ‘spider-like’

http://www.sciencephoto.com/image/15931/large/B3050233-Diatom_alga,_SEM-
SPL.jpg
Sources
 Becker, J. (1996). Diatom Reproduction. Retrieved October 26, 2012,
         from Monterey Bay Aquarium Research Institute:
         http://www.mbari.org/staff/conn/botany/diatoms/john/basics/r
         epro.htm
 Biggs, A., Kapicka, C., & Lundgren, L. (1995). Biology: The Dynamics of
         Life. Ohio: Glencoe/McGraw-Hill.
 Braby, C. E. (2001). Diatoms. Retrieved October 27, 2012, from Monterey
         Bay Aquarium Research Institute:
         http://www.mbari.org/staff/conn/botany/phytoplankton/phytop
         lankton_diatoms.htm
 Braby, C. E. (2001). Diatoms Asexual Reproduction. Retrieved October
         26, 2012, from Monterey Bay Aquarium Research Institute:
         http://www.mbari.org/staff/conn/botany/phytoplankton/phytop
         lankton_diatom_asexual.HTM


http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
Sources
 Cuvelier, D. (2005, July 8). Bacteriastrum delicatulum Cleve, 189.
         Retrieved October 27, 2012, from World Register of Marine
         Species:
         http://www.marinespecies.org/aphia.php?p=taxdetails&id=1641
         08
 Essenfield, B., Gontang, C., & Moore, R. (1996). Biology, 2nd Edition. US:
         Addison-Wesley Publishing Company, Inc.
 Fernandes, L., & de Souza-Mosimann, R. (2005, May 22). Triceratium
         moreirae Sp Nov And Triceratium dubium Triceratiaceae
         Bacillariophy. Retrieved October 27, 2012, from Biblioteca:
         http://biblioteca.universia.net/html_bura/ficha/params/title/tri
         ceratium-moreirae-sp-nov-and-triceratium-dubium-
         triceratiaceae-bacillariophyta-from/id/272002.html



http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
Sources
 Frazer, & Jennifer. (2010, March 28). Diatoms, or The Trouble With Life
         in Glass Houses. Retrieved October 26, 2012, from The Artful
         Amoeba: http://theartfulamoeba.com/2010/03/28/diatoms-or-
         the-trouble-with-life-in-glass-houses/
 Guiry, M. (2004, October 11). Triceratium moreirae. Retrieved October
         27, 2012, from Seaweed Africa:
         http://www.seaweedafrica.org/search/species/detail/?species_id
         =140330
 Guiry, M., & Guiry, G. (2002, April 23). Species Detail. Retrieved October
         27, 2012, from Algaebase:
         http://www.algaebase.org/search/species/detail/?species_id=38
         373
 Kaskel, A., Hummer, P. J., & Daniel, L. (1992). Biology: An Everyday
         Experience. USA: Merrill Publishing Company.


http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
Sources
 Kuylenstierna, M., & Karlson, B. (2006, September 15). Lithodesmium
          undulatum. Retrieved October 27, 2012, from SMHI:
          http://www.smhi.se/oceanografi/oce_info_data/plankton_check
          list/diatoms/lithodesmium_undulatum.htm
 Life XMU. (n.d.). Centric & Pennate template. Retrieved October 27,
          2012, from Life XMU:
          http://life.xmu.edu.cn/diatom/diatomphoto/Centric%20diatoms
          /htmldir/0000008e.htm
 Miller, K., & Levine, J. (2008). Biology: the Dragonfly Book. US: Prentice
          Hall.
 Oktar, A. (2011, April 1). The Microscopic living being that forms the oil
          fields: Diatom. Retrieved October 26, 2012, from Harun Yahya:
          http://harunyahya.com/en/works/40358/the-microscopic-living-
          being-that


http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
Sources
 Pece. (2011, January 20). PSYCHEDELIC DIATOMS. Retrieved October
 26, 2012, from Predict Weather @ Blogspot:
         http://predictweather.blogspot.com/2011/01/psychedelic-
         diatoms.html
 Prescott, L. M., Harley, J., & Klein, D. (2005). Microbiology. New York:
         McGraw-Hill.
 Science Photo Library. (n.d.). Jewels of the Sea. Retrieved October 26,
         2012, from Science Photo Library:
         http://www.sciencephoto.com/set/1277/jewels-of-the-sea
 Seeberg-Elverfeldt, I. A. (2004). Laminated diatomaceous sediments of
         the Red Sea, their composition and significance as recorders of
         abrupt changes in productivity and circulation during the Late
         Quaternary. Bremen, Germany.



http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
Sources
 Shin, J. (1999). Intro to Diatoms. Retrieved October 26, 2012, from
          Monterey Bay Aquarium Research Institute:
          http://www.mbari.org/staff/conn/botany/diatoms/jennifer/intro
          a.htm

 * Photo sources are inserted in the slides.




http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg

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Diatoms Bio1 Camia (7,14,27)

  • 2. CHARACTERISTICS INTRODUCING ME…
  • 3. Characteristic # 1 Diatoms are… the most abundant species in AQUATIC/ moist ecosystems http://www.jm-derochette.be/images/Diatoms/Diatoms_1.jpg
  • 4. Characteristic # 2 Diatoms are… Azpeitia tabularis Important: Photosynthetic • Fucoxanthin autotrophs http://www.sciencephoto.com/media/16036/enlarge, http://berryberryeasy.com/wp-content/uploads/2011/06/Photoautotrophs.jpg
  • 5. Characteristic # 3 Diatoms are… Psammodictyon panduriforme UNICELLULAR PROTISTS/ alga/ phytoplankton http://1.bp.blogspot.com/_C97NrIblNPQ/TTkPj0DIgZI/AAAAAAAAB38/QYLupyEthaw/s640/Diatom007.jpg, http://www.cartage.org.lb/en/themes/sciences/botanicalsciences/majordivisions/kingdomprotista/Protists/98258d .jpg
  • 6. Characteristic # 4 Diatoms are… Triceratium sp. Biddulphia tridens The most beautiful creatures on earth http://www.sciencephoto.com/image/15909/350wm/B3050211-Diatom_alga,_SEM-SPL.jpg, http://cdn.indulgy.com/78/b3/Q8/210684088788027965687KsAPSc.jpg
  • 8. Structural Characteristic # 1 Diatoms have… Functions: • Filtration/ Disinfection • Industry (eg. toothpastes, Epitheca soap) • Gives them shape (boat- like, rod-like, disk-like, Hypotheca triangle-like) • Gives them color Stichodiscus sp. Glass-like Cell walls(Frustule)with silica (siO2) http://www.sciencephoto.com/image/15918/530wm/B3050220- Diatom_alga,_SEM-SPL.jpg
  • 9. Structural Characteristic # 1 http://www.ucl.ac.uk/GeolSci/micropal/images/diat/diadiag01.gif
  • 10. Structural Characteristic # 2 Diatoms have… Functions: • Photosynthesis • Primary Food Source • Vitamin D Source Chlorophyll-like plastids inside their cells http://www.chm.bris.ac.uk/motm/chlorophyll/chphyll.gif
  • 11. Structural Characteristic # 3 Diatoms have… Functions: • Oil production • Floating • Oil Deposits under the ocean fuel Oil drops made of • Storage of chemical energy from photosynthesis sugar & starch and • Nitrogen production volutin inside their cells
  • 12. Structural Characteristic # 4 Diatoms have… Functions: • Oxygen and Nitrogen Distribution • Exchange of materials Pores with patterns on their frustules http://1.bp.blogspot.com/_C97NrIblNPQ/TTkbPZID0nI/AAAAAAAAB4s/cyjz8FLyF_g/s640/ rad_diatoms.jpg
  • 13. Structural Characteristic # 4 Diatoms have… Effects: • Immobility NO cilia and flagella Aulacodiscus oregonus http://www.sciencephoto.com/image/15915/530wm/B3050217- Diatom_alga,_SEM-SPL.jpg
  • 14. Structural Characteristic # 5 Diatoms have… Nitzschia reinholdii Different sizes Types: Actinocylcus sp. • Centric and shapes • Pennate http://2.bp.blogspot.com/_C97NrIblNPQ/TTkiNLuvrEI/AAAAAAAAB5c/9KwK-- vMrG8/s400/Diatom-2.jpg, http://www.sciencephoto.com/image/15921/530wm/B3050223-
  • 16. Habitats Diatoms live in… http://www.allabouthappylife.com/wallpaper/widescreen_wallpapers/blue_water/blue_ocea n-dsc05506.jpg, https://cmsdata.iucn.org/img/freshwater_6352.jpg
  • 17. Habitats Habitats Diatoms live in… NO cilia and flagella http://www.scienceclarified.com/images/uesc_04_img0211.jpg
  • 19. NUTRITION MAKULAY ANG BUHAY SA SINABAWANG GULAY!
  • 21. REPRODUCTION I JUST HAD… (BLEEP)!
  • 22. Asexual Reproduction The shrinking phase 1. Cell division takes place. 2. The two valves (epitheca and hypotheca) separate. 3. Each valve forms an epivalve of the daughter cell while hypovalves are secreted from the parent cells. 4. This results to one cell being the same size as the parent cell while the other is smaller. 5. This process continues until cells reach about 30%-40% of their original size.
  • 24. Sexual Reproduction The growth phase 1. Meiotic Cell division • Formation of eggs and monoflagellated sperms in the parent cell 2. Fertilization • Union of egg and sperm produces auxospore 3. Auxospore/Zygote Formation (Growth Phase) • Splitting of the parent cell while continuing to grow 4. Test Secretion • End of growth phase; new parent cell develops
  • 27. Resting Spores The inactivity phase Skeletonema marinoi When: Times of low nutrients and low sunlight What: Metabolically inactive spores How: Stored energy from photosynthetic products & thick and tough cell walls Where: At the bottom of the ocean http://images.sciencedaily.com/2011/03/110301111658-large.jpg
  • 28. ECOLOGY I WAS BORN THIS WAY!
  • 29. Ecological Role # 1 Primary producers http://oceansjsu.com/images/exped_ecoystems/arctic_marine_food_web_90.jpg
  • 30. Ecological Role # 2 Important resource • 20%-25% of the world’s carbon fixation • 40% of the world’s oxygen (in the atmosphere) • Vitamin D • 23% of primary productivity in the world • Oil/Fuel • Silicon (SiO2)
  • 31. Ecological Role # 3 Nutrient detector PLANKTON BLOOM – when there is an abundance of nutrients and excessive amount of sunlight in the ocean http://oceansjsu.com/images/exer8/plankt1.gif
  • 33. Example # 1 Triceratium moreirae Where: (mentioned) Southern Brazil Who: L.F. Fernandes and R.M. Sousa-Nosimann When: 2001 What: 47-61 μm; convex, tripolar valves; elevated with ocellus and rimoportula http://www.scielo.br/img/fbpe/rbbio/v61n1/a20fig6b.gif
  • 34. Example # 2 Lithodesmium undulatum Where: warm, temperate seas Who: C.G. Ehrenberg When: 1839 What: 37-93 μm; marginal ridge with clear pattern of perforation; undulated sides; ‘wavy’ http://www.sciencephoto.com/image/15919/530wm/B3050221- Diatom_alga,_SEM-SPL.jpg
  • 35. Example # 3 Baceteriastrum delicatulum Where: (mentioned) Mexico, Lebanon, UK, Red Sea Who: Cleve When: 1897 What: 20-40 μm; circular valves with bristles; ‘spider-like’ http://www.sciencephoto.com/image/15931/large/B3050233-Diatom_alga,_SEM- SPL.jpg
  • 36. Sources Becker, J. (1996). Diatom Reproduction. Retrieved October 26, 2012, from Monterey Bay Aquarium Research Institute: http://www.mbari.org/staff/conn/botany/diatoms/john/basics/r epro.htm Biggs, A., Kapicka, C., & Lundgren, L. (1995). Biology: The Dynamics of Life. Ohio: Glencoe/McGraw-Hill. Braby, C. E. (2001). Diatoms. Retrieved October 27, 2012, from Monterey Bay Aquarium Research Institute: http://www.mbari.org/staff/conn/botany/phytoplankton/phytop lankton_diatoms.htm Braby, C. E. (2001). Diatoms Asexual Reproduction. Retrieved October 26, 2012, from Monterey Bay Aquarium Research Institute: http://www.mbari.org/staff/conn/botany/phytoplankton/phytop lankton_diatom_asexual.HTM http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
  • 37. Sources Cuvelier, D. (2005, July 8). Bacteriastrum delicatulum Cleve, 189. Retrieved October 27, 2012, from World Register of Marine Species: http://www.marinespecies.org/aphia.php?p=taxdetails&id=1641 08 Essenfield, B., Gontang, C., & Moore, R. (1996). Biology, 2nd Edition. US: Addison-Wesley Publishing Company, Inc. Fernandes, L., & de Souza-Mosimann, R. (2005, May 22). Triceratium moreirae Sp Nov And Triceratium dubium Triceratiaceae Bacillariophy. Retrieved October 27, 2012, from Biblioteca: http://biblioteca.universia.net/html_bura/ficha/params/title/tri ceratium-moreirae-sp-nov-and-triceratium-dubium- triceratiaceae-bacillariophyta-from/id/272002.html http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
  • 38. Sources Frazer, & Jennifer. (2010, March 28). Diatoms, or The Trouble With Life in Glass Houses. Retrieved October 26, 2012, from The Artful Amoeba: http://theartfulamoeba.com/2010/03/28/diatoms-or- the-trouble-with-life-in-glass-houses/ Guiry, M. (2004, October 11). Triceratium moreirae. Retrieved October 27, 2012, from Seaweed Africa: http://www.seaweedafrica.org/search/species/detail/?species_id =140330 Guiry, M., & Guiry, G. (2002, April 23). Species Detail. Retrieved October 27, 2012, from Algaebase: http://www.algaebase.org/search/species/detail/?species_id=38 373 Kaskel, A., Hummer, P. J., & Daniel, L. (1992). Biology: An Everyday Experience. USA: Merrill Publishing Company. http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
  • 39. Sources Kuylenstierna, M., & Karlson, B. (2006, September 15). Lithodesmium undulatum. Retrieved October 27, 2012, from SMHI: http://www.smhi.se/oceanografi/oce_info_data/plankton_check list/diatoms/lithodesmium_undulatum.htm Life XMU. (n.d.). Centric & Pennate template. Retrieved October 27, 2012, from Life XMU: http://life.xmu.edu.cn/diatom/diatomphoto/Centric%20diatoms /htmldir/0000008e.htm Miller, K., & Levine, J. (2008). Biology: the Dragonfly Book. US: Prentice Hall. Oktar, A. (2011, April 1). The Microscopic living being that forms the oil fields: Diatom. Retrieved October 26, 2012, from Harun Yahya: http://harunyahya.com/en/works/40358/the-microscopic-living- being-that http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
  • 40. Sources Pece. (2011, January 20). PSYCHEDELIC DIATOMS. Retrieved October 26, 2012, from Predict Weather @ Blogspot: http://predictweather.blogspot.com/2011/01/psychedelic- diatoms.html Prescott, L. M., Harley, J., & Klein, D. (2005). Microbiology. New York: McGraw-Hill. Science Photo Library. (n.d.). Jewels of the Sea. Retrieved October 26, 2012, from Science Photo Library: http://www.sciencephoto.com/set/1277/jewels-of-the-sea Seeberg-Elverfeldt, I. A. (2004). Laminated diatomaceous sediments of the Red Sea, their composition and significance as recorders of abrupt changes in productivity and circulation during the Late Quaternary. Bremen, Germany. http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg
  • 41. Sources Shin, J. (1999). Intro to Diatoms. Retrieved October 26, 2012, from Monterey Bay Aquarium Research Institute: http://www.mbari.org/staff/conn/botany/diatoms/jennifer/intro a.htm * Photo sources are inserted in the slides. http://www.mbari.org/staff/conn/botany/diatoms/jennifer/scan11.jpg