1. Zakary Kadish

2.  Uses small pieces of DNA to identify:
 Criminals
 Parental Identity
 Bodies
 Most commonly used for court cases where
DNA is used as evidence

3.  DNA is unique to its person-much like
fingerprints
 Chances that you will find two people with the
same DNA who are not identical twins is
VERY low
 DNA is extremely reliable in identifying who a
person is, much like a fingerprint can identify
who someone is
 DNA is 99.9% similar between humans!

4.  There are 6 steps to fingerprinting:
1. Isolation of DNA
2. Cutting, sizing, and sorting of DNA
3. Transfer of DNA to nylon
4. Probing
5. Probing
6. DNA Fingerprint

5. **NOTE: THIS PROCESS WAS MADE TO DO AT HOME**
 Obtain a sample of DNA from mouth, and spit
into a shot glass
 Add 3 pinches of table salt
 Add 1 mL. of dish soap-stir for 3 minutes
 Do not make bubbles
 Pour 5 mL. of alcohol down the glass’s side
 Wait for 5 minutes
 DNA will become visible, can be extracted with
toothpick

6.  Restriction enzymes cut DNA
 DNA then sorted by size using electrophoresis
 DNA placed in gel, then electric current used to drag
pieces through the gel
 Gel is made from seaweed agarose

7.  Gel used in electrophoresis is placed on nylon
 Allowed to sit and dry overnight, so that all
liquid is evaporated, and only DNA is left

8.  Use restriction enzymes to cut DNA
 Add radioactive N-bases to DNA Pol, which
are coded for a wanted gene
 Once the DNA Pol has synthesized a new DNA
strand, the radioactive labels will be able to be
read!

9.  The DNA fingerprint can now be assembled
 Have the radioactive labels transfer to the sheet
 Align the genetic information with that of the
criminal/suspect
 You are now ready to catch a criminal!

10.  http://www.accessexcellence.org/RC/AB/BA/DNA_
Fingerprinting_Basics.php
 http://vesolt.free.fr/dna.htm