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Zahoor Ahmed
Zahoor Ahmed
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[object Object],[object Object],[object Object],[object Object],NUDRAT JAWED BSMT 3 RD YEAR, 5 TH SEMESTER
Contents ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
HORMONE RECEPTORS ,[object Object],[object Object],[object Object]
Why ER/PR testing ? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Estrogen Receptors ,[object Object],[object Object],[object Object],[object Object]
Types ,[object Object],[object Object],[object Object],[object Object]
Progesterone Receptor ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Envision System ,[object Object],[object Object],[object Object],[object Object],[object Object]
Introduction ,[object Object],[object Object]
Step by Step Envision Staining Method ,[object Object],paraffin wax coated slide Deparaffinization and Rehydration SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],tissue section Deparaffinization and Rehydration SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],Rinse Deparaffinization and Rehydration Antigen Retrieval SPECIMEN LNH 2009-013 ER/PR SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],PBS Buffer Deparaffinization and Rehydration Antigen Retrieval Rinse SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],3% Hydrogen Peroxide SPECIMEN LNH 2009-013 ER/PR Rinse Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase
Step by Step Envision Staining Method ,[object Object],PBS Buffer Rinse Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Rinse SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],ER Antibody Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Rinse Rinse SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Rinse Rinse PBS Buffer Rinse SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],Link Antibody Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Rinse Rinse Rinse Biotinylated Secondary Antibody Rinse SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Biotinylated Secondary Antibody Rinse Rinse Rinse Rinse PBS Buffer SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],Deparaffinization and Rehydration Streptavidin HRP Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Biotinylated Secondary Antibody Rinse Rinse Rinse Rinse HRP Streptavidin SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],PBS Buffer Deparaffinization and Rehydration Streptavidin HRP Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Biotinylated Secondary Antibody Rinse Rinse Rinse Rinse Rinse SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],DAB Chromogen Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Biotinylated Secondary Antibody Streptavidin HRP Rinse Rinse Rinse Rinse Rinse DAB Chromogen SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Biotinylated Secondary Antibody Streptavidin HRP DAB Chromogen Rinse Rinse Rinse Rinse Rinse Distilled Water Rinse SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],Hematoxylin Counterstain Hematoxylin Harris’s Hematoxylin Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Biotinylated Secondary Antibody Streptavidin HRP DAB Chromogen Rinse Rinse Rinse Rinse Rinse Rinse SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],Hematoxylin Counterstain Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Biotinylated Secondary Antibody Streptavidin HRP DAB Chromogen Rinse Rinse Rinse Rinse Rinse Rinse Tap Water Rinse SPECIMEN LNH 2009-013 ER/PR
Step by Step Envision Staining Method ,[object Object],Deparaffinization and Rehydration Antigen Retrieval Block Endogenous Peroxidase Primary Antibody Biotinylated Secondary Antibody Streptavidin HRP DAB Chromogen Rinse Rinse Rinse Rinse Rinse Rinse Hematoxylin Counterstain Rinse DPX Aqueous Mount SPECIMEN LNH 2009-013 ER/PR
FACTORS AFFECTING THE TECHNIQUE ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Contd. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
INTERPRETATION ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
QC/QA Measures ,[object Object],[object Object],[object Object],[object Object],[object Object]
Conclusion ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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