Chromatography Fall 09

Thin-Layer Chromatography and Column Chromatography

Background ,[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],cyclohexane petroleum ether hexane toluene dichloromethane ethyl acetate ethanol acetone methanol least polar most polar TLC vs. Column Chromatography

TLC vs. Column Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Spotting, Developing, and Visualizing ,[object Object],[object Object],[object Object],[object Object],[object Object]

Spotting, Developing, and Visualizing ,[object Object],[object Object],[object Object]

Spotting, Developing, and Visualizing ,[object Object],[object Object],[object Object],[object Object],[object Object],UV Light Iodine

Retention Factor (R f ) ,[object Object],[object Object],[object Object],[object Object],Eluent Front S A 1 Origin A 1 Rf 1 = ———— S ,[object Object],[object Object],[object Object],1 2 A 2 A 2 Rf 2 = ———— S

What You Need for TLC Experiment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

TLC Experiment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

1- Preparing a Dry Pack Column ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

2- Eluting Ferrocene ,[object Object],[object Object],[object Object],[object Object],[object Object]

3- Eluting Acetylferrocene ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Observations ,[object Object],[object Object],[object Object],Ferrocene in hexane Acetylferrocene in TBME

Experiment ,[object Object],[object Object],[object Object]

Waste Disposal ,[object Object],[object Object],[object Object],[object Object],[object Object]

Chromatography Fall 09

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Chromatography Fall 09