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Further Development of Vytex Natural Rubber
           Latex Leads to Strong Niche Market
                       Advances


           Matt Clarka (Speaker), Travis Honeycutta, Gunalan Narayananb,
                          William Doylea, Joanne Kearneya




aVystar® Corporation, 3235 Satellite Boulevard, Building 400, Suite 290, Duluth, GA 30096 USA
bStratoshift Technologies SDN. BHD., No 24, Jalan SS21/42A, Damansara Utama, 47400 Peltaling Jaya, Senlangor, Malaysia
What is Vytex® Natural Rubber Latex?

A fully commercialized source material, using a multi-patented
process and green chemistry to significantly reduce non-rubber
     components, including antigenic proteins, for a more
       consistent, stable and cleaner natural rubber latex
Vytex® NRL Technology


          FW
         L
              P            FW
                                    L
        FW
              L            P
L       P FW       P L              FW

                           FW
FW             P      FW
        P L                     L   P
    L             P                       FW     FW
                                                        FW
                                                           L
                                    L        L        L
         FW                P             L     L    L
                                         FW P FW P FW
                                                          P
                                           L P          L P
                                                 L P
Vystar® Corporation Covers the Market




Vytex Production




            4
New Standard of Latex
Vytex® NRL Meets the New ASTM D1076-10 Category 5 Standard

“Centrifuged Hevea natural latex treated with aluminum hydroxide or by
   other means, preserved with ammonia……containing less than
                     0.5 % non-rubber content.”



                                Categories
                                   1–4

                                Max. 2.0%
Vytex® NRL Advancements: Specialty Gloves

Clean Room and Specialty Gloves

    Clean room gloves made with Vytex® require
     ~ 50% less leaching to remove non-rubbers
     and chemical particles
       •   Lower product rejection rates due to extensive alkaline washes
       •   Decreased energy, water and waste usage

    Vytex® use with common antimicrobials is superior to NRL- will not
     react and de-stabilize
Protein Results in Clean Room Gloves
                        Using Comparative Leaching Processes

                                         Powder Free                 Powdered
 Vytex® NRL Trials                      Modified Lowry             Modified Lowry
 Using Normal Established                  (µg/dm²)                    (µg/dm²)
 Parameters                     Size   Spec: < 50 µg/dm2   Size   Spec: < 200 µg/dm2
 Trial #1 – Normal Process       L           17.87         XXXS         20.68
    2 pre-leach; 2 post-leach
 Trial #2                        XL          19.32          L           43.29
    2 pre-leach; 1 post-leach
 Trial #3                        S           18.30          S           68.61
    2 pre-leach only
 Trial #4                       XXXS         14.89          L           67.07
    1 pre-leach only
 Trial #5                        L           15.46          S          142.57
    No post or pre-leach

Testing conducted by Malaysian Glove Manufacturer
Vytex® NRL Advancements: Condoms
      Naturally whiter, more translucent
       condoms

      Condoms made with Vytex® NRL
       contained 35% fewer nitrosamines than traditional latex*

      Significantly reduced odor compared to NRL

      US FDA protein claim “less than 2 µg/dm2 antigenic protein”

* Customer Test Data
Vytex® NRL Advancements: Balloons




Vytex®      Control




                         Whiter, cleaner balloons for brighter,
                          truer colors with less pigment usage
                         Reduced usage of pigments and
                          whiteners (TiO2)
                         Better air and helium retention in balloons
  Vytex®    Control
Vytex® NRL Balloons: Stay Inflated Longer

                     80         Helium Retention Over Time                 Normal Air Retention Over Time

                     70
Circumference (cm)




                     60

                     50

                     40

                     30

                     20

                     10

                     0
                          12   24   36   48   60   72   84   96 108 120

                          Hours                              Vytex NRL
                                                             Control NRL
Vytex® NRL Advancements: Adhesives
Spray Adhesives

      Less non-rubber debris for cleaner spray
      Absence of non-rubber debris reduces spray-head clogging;
       decreased equipment/line downtime
      Cleaner adhesives flow easily; allowing for gravity-fed system
       vs. pump system using propellants
      ~ 40% faster tack time in some formulations compared to
       traditional latex
Antigenic Protein Results in Adhesives
                    Vytex® NRL vs. Standard NRL


                                        ELISA D6499-07 (µg/g)
         Source                    Sample 1                  Sample 2
       Vytex® NRL                     1.6                       2.4
        Control A                    624.5                     76.3




Testing conducted by LEAP Testing Services, Sayre, PA, USA
How Vytex® NRL is Helping to Solve Customer Product
              Development Challenges:
Adhesives for Personal Care Products

   Goal:
      Safe and effective skin adhesive which can be easily and
         safely removed

   Result:
      Material with excellent adhesive quality
      Low levels of protein
      Adhesive can easily be removed with soap
Vytex® NRL Advancements: Foam Products
Virtually Odor Free
       Significantly reduced leaching
       No fragrances required to mask or eliminate odor
       Vytex® process eliminates microbial food sources that
        break down to cause odor and accelerated aging

Whiter, Cleaner Foams
How Vytex® NRL is Helping to Solve Customer Product
              Development Challenges:
Customized Foams

   Goal: a greener, naturally based foam with slow recovery properties
                    4.00%
                    3.50%
                    3.00%
                    2.50%
                                                                 Typical NRL**
                    2.00%
                                                                 Vytex NRL*
                    1.50%
                                                                 Typical Polyurethane**
                    1.00%
                    0.50%
                    0.00%
                                      compression set

    * Internal test results
    **Carey, R.H. and Rogers, E.A. (1956) Modern Plastics, 33 (12), 139.
Vytex® NRL Threads: Physical Property Testing
                                                          Physical Properties
                                                                                                 Antigenic
                                                       Modulusa             Tenacitya             Protein
                                  %   Straina
                                                       (g/denier)           (g/denier)            ELISAb
           Source                                                                                (µg/dm2)

     Vytex® NRL                       929.5              0.0088                 0.31                 3.8
                                    (± 48.8)             (±0.0007)             (±0.02)




     NRL Control                      771.3              0.0144                 0.33                14.1
                                      (±90.1)            (±0.0016)             (±0.02)




       Spandex                        755.7              0.0781                 0.87                 n/a
                                    (±160.1)             (±0.0111)             (±0.07)


aTesting   conducted by North Carolina State University, College of Textiles, Raleigh, NC USA; bLEAP Testing Services.
Vytex® NRL Threads: Lower Modulus/Higher Elongation
                                       Easier Donning and Greater Comfort
                                             Tenacity vs. % Strain Curves
                            1.00



                            0.80
     Tenacity (gf/denier)




                            0.60

                                                                                     Natural Rubber Latex Control
                                                                                     Spandex
                            0.40
                                                                                     Vytex NRL


                            0.20



                            0.00
                                   0   200    400                600   800    1000
                                                    Strain (%)


  Testing conducted by North Carolina State University, College of Textiles
How Vytex® NRL is Helping to Solve Customer Product
              Development Challenges:

Specialty Fabrics for Personal Protection Products
   Goal:
      •    Latex based apparel that can worn daily
      •    Fabric must retain fluids to prevent leakage
      •    Fabric must be easy to don and comfortable to wear
      •    Production process does not include a leaching step


   Result:
      •    Low levels of antigenic proteins
      •    Tight film structure minimizes leakage
      •    Low modulus material for easy donning
Opportunities for Further Enhancements to Vytex® NRL
No Ammonia Alternative
Ammonia Preserved CL 60 is the Industry Standard
•    Current low ammonia levels to 0.20 – 0.35%
•    However, TMTD used as a preservative and therefore CL60 is not
     Nitrosamine-free

Growing demand for improved lattices:
•    Low VOCs for use in enclosed factory spaces
•    Specialty end use applications such as body paints, adhesives, etc.
•    Without ammonia or TMTD used at any stage of preservation

Very Low Ammonia Vytex NRLO5 (<.05% ammonia) currently available
Conclusions

Vytex® NRL Multi-patented Process

      Physically removes non-rubbers, including proteins,
       from liquid natural latex

      Meets new Category 5 standards of ASTM D1076-10
Conclusions

Products Made with Vytex® NRL

      Exam gloves and condoms: FDA 510(k) allowances of protein claims
      Foams: naturally whiter with minimal latex odor
      Balloons: truer colors and better air/helium retention
      Dipped goods: less leaching/improved environmental impact


   Vystar R&D focuses on addressing our customers’ varied
             product and production challenges

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International Latex Conference Presentation on Vytex NRL.

  • 1. Further Development of Vytex Natural Rubber Latex Leads to Strong Niche Market Advances Matt Clarka (Speaker), Travis Honeycutta, Gunalan Narayananb, William Doylea, Joanne Kearneya aVystar® Corporation, 3235 Satellite Boulevard, Building 400, Suite 290, Duluth, GA 30096 USA bStratoshift Technologies SDN. BHD., No 24, Jalan SS21/42A, Damansara Utama, 47400 Peltaling Jaya, Senlangor, Malaysia
  • 2. What is Vytex® Natural Rubber Latex? A fully commercialized source material, using a multi-patented process and green chemistry to significantly reduce non-rubber components, including antigenic proteins, for a more consistent, stable and cleaner natural rubber latex
  • 3. Vytex® NRL Technology FW L P FW L FW L P L P FW P L FW FW FW P FW P L L P L P FW FW FW L L L L FW P L L L FW P FW P FW P L P L P L P
  • 4. Vystar® Corporation Covers the Market Vytex Production 4
  • 5. New Standard of Latex Vytex® NRL Meets the New ASTM D1076-10 Category 5 Standard “Centrifuged Hevea natural latex treated with aluminum hydroxide or by other means, preserved with ammonia……containing less than 0.5 % non-rubber content.” Categories 1–4 Max. 2.0%
  • 6. Vytex® NRL Advancements: Specialty Gloves Clean Room and Specialty Gloves  Clean room gloves made with Vytex® require ~ 50% less leaching to remove non-rubbers and chemical particles • Lower product rejection rates due to extensive alkaline washes • Decreased energy, water and waste usage  Vytex® use with common antimicrobials is superior to NRL- will not react and de-stabilize
  • 7. Protein Results in Clean Room Gloves Using Comparative Leaching Processes Powder Free Powdered Vytex® NRL Trials Modified Lowry Modified Lowry Using Normal Established (µg/dm²) (µg/dm²) Parameters Size Spec: < 50 µg/dm2 Size Spec: < 200 µg/dm2 Trial #1 – Normal Process L 17.87 XXXS 20.68 2 pre-leach; 2 post-leach Trial #2 XL 19.32 L 43.29 2 pre-leach; 1 post-leach Trial #3 S 18.30 S 68.61 2 pre-leach only Trial #4 XXXS 14.89 L 67.07 1 pre-leach only Trial #5 L 15.46 S 142.57 No post or pre-leach Testing conducted by Malaysian Glove Manufacturer
  • 8. Vytex® NRL Advancements: Condoms  Naturally whiter, more translucent condoms  Condoms made with Vytex® NRL contained 35% fewer nitrosamines than traditional latex*  Significantly reduced odor compared to NRL  US FDA protein claim “less than 2 µg/dm2 antigenic protein” * Customer Test Data
  • 9. Vytex® NRL Advancements: Balloons Vytex® Control  Whiter, cleaner balloons for brighter, truer colors with less pigment usage  Reduced usage of pigments and whiteners (TiO2)  Better air and helium retention in balloons Vytex® Control
  • 10. Vytex® NRL Balloons: Stay Inflated Longer 80 Helium Retention Over Time Normal Air Retention Over Time 70 Circumference (cm) 60 50 40 30 20 10 0 12 24 36 48 60 72 84 96 108 120 Hours Vytex NRL Control NRL
  • 11. Vytex® NRL Advancements: Adhesives Spray Adhesives  Less non-rubber debris for cleaner spray  Absence of non-rubber debris reduces spray-head clogging; decreased equipment/line downtime  Cleaner adhesives flow easily; allowing for gravity-fed system vs. pump system using propellants  ~ 40% faster tack time in some formulations compared to traditional latex
  • 12. Antigenic Protein Results in Adhesives Vytex® NRL vs. Standard NRL ELISA D6499-07 (µg/g) Source Sample 1 Sample 2 Vytex® NRL 1.6 2.4 Control A 624.5 76.3 Testing conducted by LEAP Testing Services, Sayre, PA, USA
  • 13. How Vytex® NRL is Helping to Solve Customer Product Development Challenges: Adhesives for Personal Care Products Goal:  Safe and effective skin adhesive which can be easily and safely removed Result:  Material with excellent adhesive quality  Low levels of protein  Adhesive can easily be removed with soap
  • 14. Vytex® NRL Advancements: Foam Products Virtually Odor Free  Significantly reduced leaching  No fragrances required to mask or eliminate odor  Vytex® process eliminates microbial food sources that break down to cause odor and accelerated aging Whiter, Cleaner Foams
  • 15. How Vytex® NRL is Helping to Solve Customer Product Development Challenges: Customized Foams Goal: a greener, naturally based foam with slow recovery properties 4.00% 3.50% 3.00% 2.50% Typical NRL** 2.00% Vytex NRL* 1.50% Typical Polyurethane** 1.00% 0.50% 0.00% compression set * Internal test results **Carey, R.H. and Rogers, E.A. (1956) Modern Plastics, 33 (12), 139.
  • 16. Vytex® NRL Threads: Physical Property Testing Physical Properties Antigenic Modulusa Tenacitya Protein % Straina (g/denier) (g/denier) ELISAb Source (µg/dm2) Vytex® NRL 929.5 0.0088 0.31 3.8 (± 48.8) (±0.0007) (±0.02) NRL Control 771.3 0.0144 0.33 14.1 (±90.1) (±0.0016) (±0.02) Spandex 755.7 0.0781 0.87 n/a (±160.1) (±0.0111) (±0.07) aTesting conducted by North Carolina State University, College of Textiles, Raleigh, NC USA; bLEAP Testing Services.
  • 17. Vytex® NRL Threads: Lower Modulus/Higher Elongation Easier Donning and Greater Comfort Tenacity vs. % Strain Curves 1.00 0.80 Tenacity (gf/denier) 0.60 Natural Rubber Latex Control Spandex 0.40 Vytex NRL 0.20 0.00 0 200 400 600 800 1000 Strain (%) Testing conducted by North Carolina State University, College of Textiles
  • 18. How Vytex® NRL is Helping to Solve Customer Product Development Challenges: Specialty Fabrics for Personal Protection Products Goal: • Latex based apparel that can worn daily • Fabric must retain fluids to prevent leakage • Fabric must be easy to don and comfortable to wear • Production process does not include a leaching step Result: • Low levels of antigenic proteins • Tight film structure minimizes leakage • Low modulus material for easy donning
  • 19. Opportunities for Further Enhancements to Vytex® NRL No Ammonia Alternative Ammonia Preserved CL 60 is the Industry Standard • Current low ammonia levels to 0.20 – 0.35% • However, TMTD used as a preservative and therefore CL60 is not Nitrosamine-free Growing demand for improved lattices: • Low VOCs for use in enclosed factory spaces • Specialty end use applications such as body paints, adhesives, etc. • Without ammonia or TMTD used at any stage of preservation Very Low Ammonia Vytex NRLO5 (<.05% ammonia) currently available
  • 20. Conclusions Vytex® NRL Multi-patented Process  Physically removes non-rubbers, including proteins, from liquid natural latex  Meets new Category 5 standards of ASTM D1076-10
  • 21. Conclusions Products Made with Vytex® NRL  Exam gloves and condoms: FDA 510(k) allowances of protein claims  Foams: naturally whiter with minimal latex odor  Balloons: truer colors and better air/helium retention  Dipped goods: less leaching/improved environmental impact Vystar R&D focuses on addressing our customers’ varied product and production challenges