Biomineralization, Magnetic nanoparticles

1. “ Ferric Iron Nanoparticles Formation Mediated By Negatively Charged Polypeptides” Vamsi Krishna Aluru, Dr Robin S. Hissam Department of Chemical Engineering, West Virginia University, Morgantown, WV November 9, 2010 AICHE 2010 Annual Meeting

12. Design of Peptides AICHE 2010 Annual Meeting Name Peptide VA1 M G A V Y A Y G K S R S I Y S A Q S Y S S V S L R Y (OH) VA2 M G A V D A D G K E R D I D E A Q E D E E V D L R D (COOH) VA3 M G S E D A Y G S Y D E S Y D E Q S E D Y E S Y Y S (Combination) VAMms6 M G A V Y A Y G K S R D I E S A Q S D E E V E L R D

14. SDS-PAGE Protein purification profile of VA-Mms6, VA1 and VA2, 12.5 % separating gel, ran @100V: Lane 1) Protein ladder, Lane 2) VA-Mms6, Lane 3) VA1 and Lane 4) VA2 AICHE 2010 Annual Meeting 37 kD 25 kD 20 kD 15 kD 10 kD VA-Mms6 VA1 VA2 1 2 3 4

15. Western Blot Western Blot Stained with anti-His antibody: Lane1) purified His-tagged VA-Mms6; Lane 2) purified His-tagged VA1; Lane 3) purified His-tagged VA2 Courtesy: Dr. Yogesh Kulkarni AICHE 2010 Annual Meeting

17. Scanning Electron Microscopy (SEM) (a) SEM image of iron particles synthesized in the presence of VA-Mms6 (b) EDS of iron particles synthesized in the presence of VA-Mms6 AICHE 2010 Annual Meeting (c) SEM image of iron particles synthesized in the presence of VA2 (d) SEM image of iron particles synthesized in the presence of VA3 (c) (d)

18. Transmission Electron Microscopy (TEM) TEM images of magnetite particles synthesized in presence of : (a) VA2 (b) Mms6 VA2 *Mms6 Courtesy: Dr. Chaoying Ni, University of Delaware *Arakaki A et al. J. Biol. Chem 2003 AICHE 2010 Annual Meeting Selected Area Electron Diffraction (SAED) patterns of iron oxide particles synthesized in the presence of VA2 (a) (b)

19. Atomic Force Microscopy (AFM) (a) (b) (c) AFM image of magnetite particles synthesized in the presence of (a) VA-Mms6, (b) VA2, (c) VA3 Courtesy: Srikanth Raghavan, WVU AICHE 2010 Annual Meeting

20. X-ray Photoelectron Spectroscopy (XPS) XPS spectra of magnetite particles synthesized in the presence of VA2, VA3, VA-Mms6 and XPS spectra of standard magnetite particles 711 eV 724.4 eV VA-Mms6 Courtesy: Srikanth Raghavan, WVU AICHE 2010 Annual Meeting

21. DLS AICHE 2010 Annual Meeting Size distribution of magnetite particles synthesized in the presence of (a) VAMms6, (b) VA2, and (c) VA3 Size (d,nm) Size (d,nm) Size (d,nm) Number (%) Number (%) Number (%) a) b) c) Peak Diameter VAMms6 88 nm VA2 62 nm VA3 41 nm

22. AICHE 2010 Annual Meeting

23. Magnetic Force Microscopy (MFM) MFM image of magnetite particles synthesized in presence of VA2 (a) topography (b) phase (a) (b) Courtesy: Srikanth Raghavan, WVU AICHE 2010 Annual Meeting Individual particles of size range 40 nm

28. Thank You AICHE 2010 Annual Meeting