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2. Gene Cloning

4. DNA CLONING A method for identifying and purifying a particular DNA fragment (clone) of interest from a complex mixture of DNA fragments, and then producing large numbers of the fragment (clone) of interest.

8. PCR-amplified DNA

11. Blunt & Sticky ends

13. Cloning vectors allowing the exogenous DNA to be inserted, stored, and manipulated mainly at DNA level. 1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs

16. Plasmid vectors

22.  ZAP

27. Expression vector

32. Blue/White Color Screening lacZ lacZ insert functional enzyme nonfunctional enzyme X-gal product X-gal product

33. Selecting Colonies with Recombinant Plasmids

35. bacteriophage lambda as a cloning vector (Fig. 10.44, p. 311, Madigan et al.) transduction: transfer of host genes from one cell to another by a virus Btech7

38. End ARRIVIDIERCI………