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41. laboratory diagnosis of common fungal diseases
- 1. LABORATORY DIAGNOSIS OF COMMON FUNGAL DISEASES Prof. Louella A. Dancel, RMT, MSc. University of Perpetual Help System DALTA 42 nd PAMET ANNUAL CONVENTION Manila Hotel – December 2,2006
- 2. Clinical specimens submitted for Fungal Isolation 2004-2006* Clinical specimen 2004 2005 2006 Total Respiratory 29 35 34 98 Body fluids 37 17 34 88 Tissues 9 0 23 32 Skin 1 0 0 1 Nails 29 31 20 80 Hair 0 1 0 1 CSF 8 5 8 21 Others 5 20 24 49 TOTAL 118 109 153 380
- 3. Fungal Isolates 2004-2006 * Isolate 2004 2005 2006 C.albicans 5.1% 13.8% 11.8% C.tropicalis 1.8% 3.7% 5.2% C.parapsilosis 5.1% 6.4% 3.9% C.glabrata 0.91% 0.91% 3.9% C.famata 1.8% 4.6% 2.0% Aspergillus spp. 0.91% 0% 0.65% Fusarium spp. 0% 0% 0.65%
- 35. Black piedra - distribution Superficial mycoses
- 59. Classification of Dermatophytes
- 60. Microsporum Macroconidia Rough walled Microconida present
- 61. Trichophyton Macroconidia Smooth walled Microconidia present
- 62. Epidermophyton Chlamydoconidia Macroconidia Smooth walled Microconidia none
- 77. Skin scraping specimen Wipe with water www.doctorfungus.org scalpel Paper / envelope active edge
- 79. KOH of skin scrapings Cutaneous Septate hypha
- 80. KOH of skin scrapings Cutaneous arthrospores septate hypha
- 89. Mycology
Notas do Editor
- Cutaneous mycoses are fungal infections of the integument and its appendages. They are superficial infections of the skin, hair & nails The fungus localizes only in the cornified layer, stratum corneum, No living tissue is invaded Pathological changes occur in the host because of her reaction to the fungus and its products of metabolism or life. A llergic & inflammatory eczematous host response
- Dermatophytosis are caused by a closely related group of organisms, called dermatophytes Which are keratinophilic Very similar & closely related group of fungi that cause a wide variety of clinical conditions A single species may be involved in several disease types, each with its distinctive pathology Most commonest infectious agents of man, the infections are distributed worldwide (geographically & racially) Common term is ringworm
- Dermatomycosis are skin infections that are caused neither by dermatophytes nor by the genus candida. They may be caused by soil inhabiting fungi
- Tinea capitis is a dermatophyte infection of the scalp, eyebrows and eyelashes caused by species of Microsporum and Trichophyton. type & severity of response is related to the species & strain of dermatophyte causing infection
- Tinea capitis is a dermatophyte infection of the scalp, eyebrows and eyelashes caused by species of Microsporum and Trichophyton. type & severity of response is related to the species & strain of dermatophyte causing infection
- Favus refers to the similarity of appearance of scutula and honeycombs. Favus is characterized by the occurrence of dense masses of mycelium & epithelial debris which form yellowish, cup-shaped crusts called SCUTULA. The scutulum develops in a hair follicle, with the hair shaft in the center of the raised lesion. Removal of these crusts reveals an oozing, moist red base. After a period of years, atrophy of the skin occurs, leaving a cicatricial alopecia & scarring. Scutula may be formed on the scalp or the glabrous skin.
- Tinea corporis is a dermatophyte infection of the glabrous skin most commonly caused by species of the gnera Trichophyton and Microsporum . The infection is generally restricted to the stratum corneum of the epidermis. The clinical symptoms are a result of the fungal metabolites acting as toxins & allergens. Lesions vary from simple scaling, scaling with erythema & vesicles, to deep granulomata. Villous hair in the involved area may be invaded, & the follicle often acts as a reservoir for recrudescence of the disease.
- Tinea corporis is a dermatophyte infection of the glabrous skin most commonly caused by species of the gnera Trichophyton and Microsporum . The infection is generally restricted to the stratum corneum of the epidermis. The clinical symptoms are a result of the fungal metabolites acting as toxins & allergens. Lesions vary from simple scaling, scaling with erythema & vesicles, to deep granulomata. Villous hair in the involved area may be invaded, & the follicle often acts as a reservoir for recrudescence of the disease.
- Tinea imbricata is a geographically restricted form of tinea corporis caused by Trichophyton concentricum. It is characterized by polycyclic, concentrically arranged rings of papulosquamous patches of scales scattered over and often covering most of the body
- Tinea barbae is a dermatophyte infection of the bearded areas of the face and neck, and therefore is restricted to adult males.
- Tinea cruris is a dermatophyte infection of the groin, perineum, & perianal region, which is acute or chronic and generally pruritic. The lesion is sharply demarcated, with a raised, erythematous margin & thin, dry epidermal scaling. The disease is found in all parts of the world but is more prevalent in the tropics. It tends to occur when conditions of high humidity lead to maceration of the crural region. A similar condition may involve the axilla or other intertriginous areas. The disease is more common in men, but frequently involves women, usually when it is transmitted by intimate contact or fomite. It may reach epidemic proportions in athletic teams, troops, ship crews, & inmates of institutions. In such cases, it is probably most commonly transmitted by towels, linens, & clothing. E. Floccosum has been isolated from blankets & sheets as well as rugs & other fomites where it can survive for years as infective arthroconidia.
- Tinea pedis is a dermatophyte infection of the feet involving particularly the toe webs and soles. The lesions are of several types, varying from mild, chronic, and scaling to acute, exfoliative, pustular, & bullous disease.
- Most dermatophyte infections of the hand, particularly of the dorsal aspect, are similar to tinea corporis. Tinea manuum refers to those infections in which the interdigital areas & the palmar surfaces are involved & show characteristic pathologic features. Along with tinea pedis, tinea manuum is one of the commoner types of chronic dermatophytosis in the adult. It has been postulated that this is related to the lack of sebaceous glands & their fungistatic lipids in these two areas.
- Tinea unguium is an invasion of the nail plates by a dermatophyte. Onychomycosis is an infection of the nails caused by nondermatophytic fungi and yeasts. Tinea unguium is of at least 2 types: Superficial white onychomycosis – invasion is restricted to patches or pits on the surface of the nail invasive subungual dermatophytosis (ringworm of the nail), in which the lateral or distal edges of the nail are first involved, followed by establishment of the infection beneath the nail plate.
- For skin specimen, first clean lesion & periphery with 70% alcohol. Use sterile scalpel or edge of microscope slide, scrape perpendicular to the skin. Scrape around the active edge where the fungus is actively growing. The center of lesion heals first, so the laboratory results are negative using this sample. If the lesion is inflamed or with fissures, clean it with sterile distilled water. Collect skin scrapings in paper envelope or petri dish, or place between 2 slides. Store at room temperature. If patients are young children and are scared of the scalpel, use can use scotch tape to collect specimen for microscopy. Collect moist exudate for candida
- These Fungi exhibit characteristic structures in clinical specimens that can be seen microscopically using a brightfield or phase contrast microscope. You can prepare a wet mount, a specimen plus sterile water or NSS, or specimen alone, like exudates. We can use potassium hydroxide, from 10 to 30% depending upon the type of specimen, for skin use 10%, for nails use a stronger concentration, 30%. Potassium hydroxide digests or dissolves proteins, fats & carbohydrates, the tissue clears Making the fungal cell wall, which is resistant to alkali, visible. Addition of Parker blue-black ink, will stain the fungal structures, facilitating microscopic examination, fungi appear bluish green. The preparation is passed 2 or 3 times over an alcohol lamp or bunsen burner, to hasten the reaction. If the result is negative, especially in nail scrapings, you can leave the preparation overnight on the lab table, to give time for digestion to occur. View the preparation again the next day. Question: Can you use Parker permanent blue or black ink? YES
- Microscopic examination
- Use several media