Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Anti-TIMP-1 antibody (STJ96023)- http://www.stjohnslabs.com/timp-1-antibody?filter_name=STJ96023 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Rating:
Specificity:
ANTIBODY CUSTOMER REVIEW
Findings: “Good signal, cost effective (1:2000),
gives several unspecific bands between 30-60
kD.” – Gabor Kokeny, Semmelweis University.
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Protocol
Gel
Electrophoresis
12% polyacrylamide gel,
constant voltage 150 V, 60 min.
Membrane
Wash
1X TBST wash for 3 times.
Transfer 0.45 nm nitrocellulose
membrane, 1 piece of gel (wet
transfer), constant current 250
mA 60 min.
Secondary
Antibody
Probing
secondary antibody was added to
the solution of 1X TBST with 5%
skimmed milk powder with
1:2000 dilution ratio, for 1 hr.
Blocking 1X TBST with 5% skimmed milk
powder for 1 hr.
Membrane
Wash
1X TBST wash for 3 times.
Primary
Antibody
Probing
primary antibody was added to
the solution of 1X TBST with
2% BSA, 4˚C for overnight
Visualisation ECL visualization for 1 min (the
visualization time was
determined by actual result. It
was ensured that the
visualization time was identical
for the same antigen).
Model Number STJ96023
Antibody Name Anti-TIMP-1 antibody
Application Western Blot
Species Mouse
Tissue Kidneys were
homogenized in RIPA
buffer containing
protease inhibitor
cocktail (Roche), equal
volume of 2X Laemmli
buffer was added then
boiled for 5 min in water
bath at 95˚C.
Dilution 1:2000
Secondary Antibody HRP goat anti-rabbit
Secondary Dilution 1:2000 ß
In both control and
fibrotic mouse
kidneys there were
target bands detected
by STJ96023 Timp-1
antibody at 23 kD,
representing TIMP-1.
The fibrotic kidney
was more positive
(positive control).
(Figure: Lane 1:
control kidney; Lane
2: fibrotic kidney).