Customer Review for Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)

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Acetyl-Histone H2A (Lys5) Antibody detects endogenous levels of histone H2A only when acetylated at lysine 5. To purchase this antibody use the following link: http://www.stjohnslabs.com/histone-h2a-acetyl-lys5-antibody?filter_name=STJ97171

Ciências

HOW DID OUR
ANTIBODY PERFORM?
CUSTOMER REVIEW: Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)

WHAT WAS USED?
Primary antibody: STJ97171. Histone H2A
(Acetyl Lys5) Polyclonal Antibody.
Provider: St John's Laboratory
Dilution ratio: 1:250
Application: Immunofluorescence
Materials for Validation: Drosophila 3rd instar
imaginal discs

WHAT WAS THE PROTOCOL?
Fixation: 4% paraformaldehyde in PBS + 0.1% Triton-X for 5
minutes.
Blocking: 5% FBS in PBS + 0.4% Triton-X for 45 minutes.
Primary antibody probing: primary antibody was added to
the solution of blocking buffer, 4°C overnight.
Wash: PBS + 0.4% Triton-X wash for 3 times.
Secondary antibody probing: secondary antibody (Alexa-
conjugated goat anti-rabbit 568) was added to the blocking
buffer with 1:500 dilution ratio, for 2 hrs.
Wash: 1X PBS + 0.4% Triton-X wash for 3 times.
Mounting: DAPI-staining conducted in PBS for 10 minutes,
followed by mounting with Prolong Gold Antifade.
Visualization: Deltavision fluorescence microscope.

WHAT WAS THE PROTOCOL?
Fixation: 4% paraformaldehyde in PBS + 0.1% Triton-X for 5 minutes.
Blocking: 5% FBS in PBS + 0.4% Triton-X for 30 minutes.
No. Antigen Primary antibody
Primary
antibody
dilution ratio
Secondary
antibody dilution
ratio
Co-staining
Antibody
Mounting Medium
1
Diploid imaginal
disc cells
STJ97171. Histone H2A
(Acetyl Lys5) Polyclonal
Antibody
1:250 1:500 DAPI Prolong Gold

WHAT DID THE CUSTOMER THINK?
Testing results were provided by Serafin Colmenares from
Lawrence Berkeley National Laboratory. They stated that
“H2AK5Ac signal overlaps with complete nucleus, except
for DAPI bright heterochromatic region. This is in line
with the expected signal for this specific modification.”

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Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Anti-GAPDH -http://www.stjohnslabs.com/gapdh-antibody-p-98566 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67). Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore, it is associated with ribosomal RNA transcription.[5] Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. Anti-Ki67 -http://www.stjohnslabs.com/ki-67-antibody-p-92897 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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