3. Ghana just went through the first
wave of Covid-19 infections...
4. Covid-19 Diagnostic process using open source
technology and reagents with the Bio.Kitchen Mobile Lab
Patient
sample
Virus
RNA
Corona Patient sample
RNA-extraction is run
using Oxgen kits. The
RNA is available after
45mins.
Virus RNA is inserted into
heatblocks for a LAMP
quick test (research use
only) with results within 30
min.
Reagents
Patient
Diagnosis
5. Severe global shortage of testing plastic consumables in
the markets: Ghana has big problems importing materials.
6. UnternehmerTUM GmbH / 21.11.19
With 1 station per 100 000 citizens our mobile lab allows
to compensate the lack of testing capacity in Ghana.
We need locally produced plastic consumables to keep up with consumption.
Per day we need approx:
● 10000 pipette tips
● 300 swabs
● 300 spin columns
● 2000 1.5ml tubes
● 1000 0.2ml tubes
Production has to be effortless, flexible, cheap, RNA free
and product has be packaged ready to use.
Join our deep dive tomorrow 16-18.00 to learn more.
Maximum capacity:
300 diagnoses per station per 24h
8. UnternehmerTUM GmbH / 21.11.19
Research use only LAMP quick test give results in 1.5h
without the need for thermocyclers or centrifuges
https://international.neb.com/protocols/2016/08/15/warmstart-colorimetric-lamp-2x-master-mix-typical-lamp-protocol-m1800
http://oxgen.ge/product/pcr-fragment-purification/
0. Sample collection by
medical staff.
The patient sample is taken
with a swab, packaged and
labelled. Patient data is
stored with a smartphone.
QR code labels are used
throughout the process.
1. Virus RNA extraction
using a simplified virus lysis
and RNA purification
protocol in 45 mins without
the need for a centrifuge.
2. RNA amplification
is done with a isothermal
LAMP molecular biology
test kits in 30 mins using a
heatblock instead of a
thermocycler.
3. machine result readout
is ready after 1h with
minimal hands on time. QR
codes link the results to
the patient data.
Documentation happens by
smartphone photo and is
stored offline. Positive
results should be
confirmed with QPCR
9. UnternehmerTUM GmbH / 21.11.19
certified qPCR test protocol give
high quality results in 3h in the mobile lab
https://www.chaibio.com/openqpcr
0. Sample collection by
medical staff.
The patient sample is taken
with a swab, packaged and
labelled. Patient data is
stored with a smartphone.
1. Virus RNA extraction
virus lysis and RNA
purification using
established protocols with a
centrifuge in 45mins. QR
code labels are used
throughout the process.
2. QPCR RNA
amplification
is done with a reverse
transcription and QPCR
protocol and CE certified
molecular biology test kits
in 2h.
3. machine result readout
is ready after 2h. QR codes
link the results to the
patient data.
Documentation happens by
smartphone photo and is
stored offline.
