Introduction to Oxidative Stress and its role

1. Introduction to Oxidative Stress
Shruti Suhas Varode
PRN 20210902023

2. What is a Free Radical ?
https://www.cellgs.com/blog/free-radicals-
vs-reactive-oxygen-species-whats-the-
difference.html

3. Damage by free radicals
https://twitter.com/hiba_alzoubi/status/10
74829633605042177

4. Reactive Oxygen Species
Superoxide ion Hydrogen
peroxide
Hydroxyl ion
http://www.chemspider.com

5. Production
of free
radicals
Endogenous ETC
NOS
NADPH Oxidase
Xanthine Oxidase
PGs synthesis
Lipid peroxidation
Auto-oxidation of metals
Exogenous Alcohol
Ionising Radiation
Drug metabolism
Smoking

6. Electron Transport Chain

7. Inflammation

8. Ionising Radiation

9. Metal Build-up and auto-oxidation

10. Lipid peroxidation

11. Cellular Defence
Mechanisms
(Anti-oxidants)
Enzymatic
• Superoxide dismutase
• Catalase
• Glutathione peroxidase
• Glutathione reductase
Non- enzymatic
• Vitamin A
• Vitamin C
• Vitamin E

12. Superoxide Dismutase

13. Catalase
Basic Structure of a peroxisome

14. Glutathione and related enzymes

15. Cellular location of anti-oxidants

16. Balance between pro-oxidants and anti-oxidants
Pro-oxidants Anti-oxidants
Glutathione and
related enzymes
SOD, Catalase
Vitamin A, C, E
Superoxide anion
Hydrogen
peroxide
Hydroxyl radicals
Oxidative Stress ROS lacking

17. By Dan Cojocari - Own work, CC BY-SA 4.0,
https://commons.wikimedia.org/w/index.p
hp?curid=46529393

18. Thank you!