This document discusses in silico PCR, which is a computational technique for simulating PCR reactions without physically performing experiments in a lab. It allows researchers to predict amplicon size and location, and assess primer specificity. The key benefits of in silico PCR include cost-efficiency, time savings, experimental design assistance, and quality control. The document provides details on the protocol for conducting in silico PCR using various bioinformatics tools. It also outlines some common applications of PCR such as DNA amplification, molecular cloning, and gene expression analysis.