This ppt is quite helpful for students/ researchers to understand the mechanism behind ethosomes penetration in the skin barrier when applied topically as well as it helps you to brief on drug detailing while formulating the ethosomes formulation. for any more question you want to ask, feel free to contact: shikhasingh_ss@yahoo.com thank you!

1. ETHOSOMES AS NOVEL DRUG
DELIVERY SYSTEM
Presented by Ms. SHIKHA Y. SINGH
Under the guidance of Prof. SMITA.S AHER
Kalyani charitable trust’s
Ravidra gambhirrhao Sapkal
Anjaneri, Nashik-422213

2. CONTENTS
 INTRODUCTION
 ETHOSOMES
 COMPOSITION
 MECHANISM OF ACTION
 ADVANTAGES & DISADVANTAGES
 CHARACTERIZATION
 METHODS OF PREPARATION
 APPLICATION
 CONCLUSION
Presentaionby-ShikhaYSingh

3. INTRODUCTION
Why we need NDDS?
What is NDDS?
What are the approaches used in
NDDS?
Why TDDS ?
What is vesicular system?
What is Ethosomes?
Presentaionby-ShikhaYSingh

4. ETHOSOMES
 Ethosomes are “Ethanolic liposomes”.
 Ethosomes were developed by touitou, 1997
 Ethosomes are non-invasive delivery carrier that
enable drugs to reach the deep skin layers and / or
systemic circulation.
 “Soft vesicles” represent novel vesicles carrier for
enhanced delivery through the skin.
 Size of ethosomes vesicles-30nm to few microns.
Presentaionby-ShikhaYSingh

5. COMPOSITION
It mainly comprises of
Phospholipid- PC, PA, PPG, PS etc
Alcohol- ethanol & isopropyl alcohol
Water
Polyglycol or glycol
Cholesterol
Dye- rodamine & FITC
Vehicle - Carbapol
Presentaionby-ShikhaYSingh

6. SKIN MECHANISM AFTER DRUG APPLICATION
Presentaionby-ShikhaYSingh

7. SKIN MECHANISM AFTER DRUG APPLICATION
Presentaionby-ShikhaYSingh

8. ETHANOL PENETRATION
Presentaionby-ShikhaYSingh

9. ADVANTAGES:
 Ethosomes enhance permeation of the drug through skin transdermal &
dermal delivery.
 Ethosomes are platforms for the delivery of large & diverse groups of
drugs (peptides, protein molecules)
 Ethosomal systems are much more efficient at delivering a fluorescent
probe (quantum dots) to the skin in terms of quantity & depth.
 Low risk profile- the technology has no large scale drug development
risk, as the toxicological profiles of the ethosomes components are well
documented in the scientific literature.
 High patient compliance- the ethosomes drugs are administered in a
semisolid form (gel/cream), producing high patient compliance. In
contrast, iontophoresis & phonophoresis are relatively complicated to use,
which will affect patient compliance.
 High market attractiveness for products with proprietary technology.
Relatively simple to manufacture with no complicated technical
investments required for the production of ethosomes.
 The ethosomes system is passive, non-passive & available for immediate
commercialization.
Presentaionby-ShikhaYSingh

10. LIMITATIONS:
 Poor yield.
 In case if shell locking is ineffective then the ethosomes may
coalescence and fall apart on transfer into water.
 Loss of product during transfer form organic to water media.
Future perspective:
 Introduction of ethosomes has intiated new area in transdermal
drug delivery
 Further research in this area will allow better control over drug
release in vivo allowing physicians to make therapy more efficient.
 It offers good opportunity for non-invasive delivery of small-,
medium- & large-sized drug molecules.
 Special emphasis given to skin delivery of proteins & other
macromolecules & for transcutaneous immunization.
Presentaionby-ShikhaYSingh

11. CHARACTERISATION METHODS:
Vesicle shape- Transmission electron microscopy (TEM), Scanning electron microscopy( SEM)
Entrapment efficiency- Mini column centrifugation method; Fluorescence spectrophotometry
Vesicle size- Dynamic light scattering method
Vesicle Skin interaction study- Confocal laser scanning microscopy; Fluorescence microscopy; TEM.
Eosin-Hematoxylin staining
Phospholipid-ethanol interaction- 31P NMR; Differential scanning calorimeter
Degree of deformability- Extrusion method
Zeta potential- Zeta meter
Turbidity- Nephalometer
In vitro drug release study- Franz diffusion cell with artificial or biological membrane, Dialysis bag diffusion
Drug deposition study- Franz diffusion cell
Stability study- Dynamic light scattering method & TEM
Presentaionby-ShikhaYSingh

12. METHOD OF PREPARATION
Phospholipid + Drug + Other lipid material
Dissolve in Ethanol in covered vessel with vigorous stirring at room temperature
Add Propylene Glycol at 40°C during stirring
Heat mix upto 30°C
Add water at 30°C
Stir for 5 minuets in covered vessel
Size reduction by sonication or extrusion
Store under refrigeration
COLD METHOD
Presentaionby-ShikhaYSingh

13. Disperse Phospholipid in
water at 40°C
Ethanol + Propylene Glycol at
40°C
Mix organic phase to aqueous phase
Add drug dissolved in suitable solvent (Water
or Ethanol depending on solubility)
METHOD OF PREPARATION
INJECTION METHOD
CLASSIC DISPERSION METHOD
HOT METHOD
Presentaionby-ShikhaYSingh

14. APPLICATION
 Delivery of Antiviral drugs
 Topical delivery of DNA
 Transdermal delivery of Hormones.
 Delivery of Anti-parkinsonism agent
 Transcellular Delivery
 Delivery of Anti-Arthritis Drugs
 Delivery of Problematic drug molecules
 Delivery of Antibiotics
 Delivery of Antigen loaded Drugs
 Delivery of NSAIDS
 Widely used in Cosmoceuticals
Presentaionby-ShikhaYSingh

15. CONCLUSION
 The main limiting factor of transdermal drug
delivery system i.e. epidermal barrier can be
overcome by ethosomes to significant extent.
 The ethosomes more advantages when compared
to transdermal and dermal delivery.
 Ethosomes are the non invasive drug delivery
carriers that enable drugs to reach the deep skin
layers finally delivering to the systemic circulation.
 It delivers large molecules such as peptides,
protein molecules. Simple method for drug delivery
in comparison to Iontophoresis and Phonophoresis
and other complicated methods.
 High patient compliance as it is administrated in
semisolid form (gel or cream) and various
application in Pharmaceutical, Veterinary, Cosmetic
field.
Presentaionby-ShikhaYSingh

16. ANY QUERIES???
Presentaionby-ShikhaYSingh

17. Presentaionby-ShikhaYSingh