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Primer Designing

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mohammad shenagari
mohammad shenagari
Tecnologia
1 de 34
Primer Design
1. Gene Runner 2. Oligo 6 4. Manual 3. Primer 3(on line)
Gene Runner 1.Sequence Insertion
 
 
 
 
 
 
Oligo 6
 
 
 
 
 
 
Manual 1. Reference sequence & other sequences (NCBI) 2. Alignment (Mega 4) 3. Sequence selection (In selected gene) 4. Blast (NCBI) 5. TM ( oligo analyzer , oligo6, gene runner) 6. Secondary structures (loop, hairpin,…) 7. False priming (oligo 6)
HCV Genome
Alignment
 
Current oligo length Position number
Non specific band
 
 
 
 
- Length ,[object Object],[object Object]
 
 
 
 
 
 
 

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Primer Designing