SHAPE Society

1. MICE MRIMICE MRI
New Data

2. AIMSAIMS
This study is for imaging aortic
atherosclerotic plaques in Apo E deficient
mice
In addition to study of Apo E
atherosclerotic mice this effort is meant to
provide necessary technical experiences for
rabbit study soon to be approved and
transferred to Galveston

4. MRIMRI
withwith
Contrast (Magnevist)Contrast (Magnevist)
In order to be sure that we are looking to
the aorta,we decided to inject contrast
media
We had to inject Magnevist to mouse while
inside the magnet, so we tried to cannulate
femoral vein, jugular vein, intracardiac
approach

5. In order to decrease the motion effect of
respiration,we had to use respiratory gated
MRI
To have respiratory gating,we needed to
Intubate the mouse and use ventilation

29. The MR Image characteristicsThe MR Image characteristics
Coil:5 and 9cm
Field of view:6 cm
Matrix(nv):256
Spin Echo:Tr=2500,Te=12, Slice=17-24
Movi(while injecting the Gadelenium) :
Tr=200, Te= 5

30. 3/103/10

31. MRI Parameters forMRI Parameters for
Movi ImagesMovi Images
After injection of Magnivest
Tr=200, Te=5

33. Soft ware analysis to show theSoft ware analysis to show the
flow and find the aortaflow and find the aorta

37. Spin Echo Images:Spin Echo Images:
Tr=2500, Te=12Tr=2500, Te=12
nv=128nv=128

40. High resolution spin echoHigh resolution spin echo
imagesimages
Slice thickness :1.6 ,Gap:0.5Slice thickness :1.6 ,Gap:0.5
Tr=2500, Te=12, nv=256Tr=2500, Te=12, nv=256

42. 03/1303/13

48. 3/14mvcor3/14mvcor

50. Sems tranSems tran

54. SPIO InjectionSPIO Injection
With injection of Feridex with maximum
dose(3µmol/kg),we had some problems:
because of small blood content of mouse,
dilution wasn’t enough
It caused too much decrease in T2 , so
although we had approximately good
resolution, we think that by decreasing the
dose we can find better images

55. Future PlansFuture Plans
Injection of 40µmol/kg of Feridex
ECG gated MRI (we have the motion effect
of heart in chest region)
Starting rabbit study