1. Splenocyte Isolation

2. Splenocyte Isolation
Purpose: To define and understand
the lymphatic system and its
components, and to purify and
culture splenocytes.

3. Splenocyte: any one of the different white blood
cell types that are situated in the spleen or
purified from splenic tissue.
Splenocytes generally consist of phagocytic
macrophages, antigen-presenting cells, and
mature T and B lymphocytes.

4. The lymphoid system or
lymphatic system: consists of all
of the tissue aggregates and
organs composed of lymphoid
tissue which function together to
produce specific resistance to
disease (immunity).

6. Primary lymphoid organs Secondary lymphoid organs
site of lymphopoiesis provide interaction of
acquire Ag receptors
lymphocytes with themselves,
selected for tolerance to self-Ags accessory cells, and Ag
Ex: Thymus (T) and fetal liver
disseminate immune responses
and bone marrow (B)
Ex: Lymph nodes and the spleen

7. fetal
liver

9. Disseminate: to distribute or spread something,
especially information, or become widespread.
Used within this context, secondary lymphoid
organs are capable of dispatching the appropriate
WBCs to the site of infection.

10. Lymph nodes and the spleen are the most highly
organized of the secondary lymphoid organs.
Lymph nodes: the sites where immune responses
are mounted to antigens in lymph.
The spleen: a secondary lymphoid organ that
specializes in filtering blood and trapping blood-
borne antigens.

11. Lymph nodes Spleen
responses mounted against responses mounted against
Ag in lymph blood-borne Ag
filter Ag in lymph filter Ag in blood
specialized for local can respond to systemic
infections infections
Ag supplied by lymphatic
Ag supplied by splenic artery
system

13. Lymphopoiesis (lymphocyte development): a
process occurring within primary lymphoid organs
in which lymphocytes differentiate from lymphoid
stem cells, proliferate, and mature into functional
cells.

14. fetal
liver

15. Systemic infection: a generic term for infection
caused by microorganisms in animals or plants,
where the causal agent (the microbe) has spread
actively or passively in the host's anatomy, and
is disseminated throughout several organs in
different systems of the host.