This is the new In-Spec catalog released 3/08/11. Take a look!

1. Spectrophotometric
Standards Reference ®
Guide
NIST Traceable
CHEMICALS Customizable
®
No Mixing or Diluting

2. Ordering Information
Customer service representatives and technical support are
Contents
Table of Contents
available Monday through Friday from 7:30 am to 4:00 pm
Continuous Improvement 4-5
Eastern Time. Orders may be placed by phone, e-mail or fax.
Every order receives an order confirmation that includes items In-Spec® UV Standards 5-6
ordered, pricing, shipping method and estimated shipping date.
In-Spec® Visible Standards 5-6
GFS Chemicals, Inc. In-Spec® Patent Blue Standards 8
800 Kaderly Drive In-Spec® Amaranth Red Standards 9
Columbus, OH 43228
In-Spec® BCA Purple Standards 10
800-838-5567 extension 121 (U.S. and Canada)
740-881-5989 (Fax) In-Spec® Nano-Kit 11
001-740-881-5501 (International) Wavelength Standards 12-13
in-spec@gfschemicals.com (e-mail)
Stray Light Filters 14
www.in-spec.com Potassium Dichromate 15
www.gfschemicals.com 16
Custom Standards
Opening an Account Amco Clear®Turbidity Standards 17
GFS Customer Service Representatives will be glad to help you Application Notes 18-20
open an account. For terms and conditions see Inside Back
Troubleshooting Guide 21
Cover.
For complete Terms and Conditions, please visit: Analytical & Lab Chemicals 22
http://www.gfschemicals.com/statics/documents/aboutus/
termsandconditions.html
Ordering
2 3

3. Continuous
GFS Chemicals has been manufacturing standards for
over twenty years for unique customer applications.
The In-Spec® product line is
constantly growing and currently
includes standards to check not just
photometric accuracy but also wave-
length, stray light, and resolution for
a variety of instrumentation.
Many of our standards are specific to your industry.
Some recent areas of focus have
been short pathlength instruments
and microplate readers used in
SeTTINg The STaNDarD for many pharmaceutical and biotech
SpeCTrophoToMeTrIC laboratories.
aNalySIS & verIfICaTIoN
Continuous improvement at GFS
Chemicals means:
• Development of new standards
for the latest instruments.
• R&D for new uses and ®
applications for the In-Spec®
product line.
• Investment in a new laboratory Features Benefits
to meet higher quality control Non-Toxic No disposal fees
standards for the demands of Green Alternative
the most stringent industries. Inexpensive Save cost compared to
• Certification for ISO 17025 is sealed cuvette alternatives
underway. Easy to Use No wasted time with dilutions
No special precautions
• Experienced technical support
to troubleshoot and answer your NIST Traceable Confidence in instrument readings
Meets regulatory requirements
specific questions.
Live Technical Assistance Assistance for method development
Help with troubleshooting
Customizable Made for your application
Multiple pathlengths and wavelengths
available
Manufactured by GFS In stock and ready to ship
Trusted name for quality in the industry
4 Improvement 3
5

4. Selection Gui de
In-Spec® How to Use:
Choose your wavelength of interest then go across the chart
Photometric
to find your closest target absorbance value. The top of the
Standards column will identify the item number and product name
Selection Guide associated with that value.
UV Standards
Wave- Uv-1 Uv-2 Uv-3 Uv-4 Uv-5 Uv-6 Uv-7 Uv-8 Uv-20 Uv-30
length #8301 #8302 #8303 #8304 #8305 #8306 #8307 #8308 #8309 #8310
250 0.086 0.127 0.177 0.305 0.347 0.447 0.513 0.570 1.135 1.664
254 0.081 0.120 0.165 0.283 0.323 0.416 0.477 0.528 1.054 1.551 In-Spec® UV In-Spec® Visible
260 0.075 0.112 0.152 0.259 0.297 0.382 0.437 0.484 0.967 1.420 Standards Standards
280 0.056 0.870 0.112 0.188 0.220 0.283 0.322 0.355 0.715 1.058 In-Spec® UV Standards are de- The In-Spec® Visible Standards
300 0.039 0.060 0.79 0.131 0.155 0.201 0.230 0.253 0.508 0.754 signed to check the photometric are designed to check the pho-
350 0.011 0.030 0.042 0.072 0.086 0.110 0.127 0.139 0.284 0.420 accuracy of a traditional spectro- tometric accuracy of a traditional
photometer from 250nm up to spectrophotometer from 350nm
400 0.007 0.018 0.026 0.045 0.054 0.069 0.080 0.088 0.179 0.266
400nm. up to 850nm.
Visible Standards UV Standards - Item Numbers Visible Standards-Item Numbers
UV-1: 8301 Vis 0.05: 8311
Wave- vIS-0.5 vIS-0.1 vIS-0.3 vIS-0.5 vIS-0.8 vIS-1 vIS-4
UV-2: 8302 Vis 0.1: 8312
length #8311 #8312 #8322 #8313 #8314 #8315 #8316
UV-3: 8303 Vis 0.3: 8322
400 0.122 0.260 0.724 1.254 1.882 2.436 3.43
UV-4: 8304 Vis 0.5: 8313
450 0.082 0.177 0.500 0.871 1.323 1.780 3.326 UV-5: 8305 Vis 0.8: 8314
500 0.059 0.126 0.360 0.628 0.957 1.298 3.238 UV-6: 8306 Vis 1.0: 8315
550 0.043 0.093 0.270 0.470 0.719 0.975 3.12 UV-7: 8307 Vis 4.0: 8316
600 0.034 0.072 0.212 0.368 0.566 0.767 2.91 UV-8: 8308
UV-20: 8309
650 0.027 0.056 0.169 0.294 0.450 0.611 2.524
UV-30: 8310
700 0.022 0.045 0.137 0.239 0.365 0.497 2.111
Available as sealed quartz cuvettes or bottles (60mL, 125mL, 250mL,
750 0.018 0.037 0.111 0.195 0.299 0.406 1.740
500mL, 1L)
800 0.017 0.030 0.092 0.161 0.247 0.334 1.440 If you need a more precise value please see the custom
Please note that the values in the table are representative values only and are to assist in picking out the appropriate standard for
products section on page 16.
your application. The actual value of the lot you receive may vary. The data provided is representative of a 10mm pathlength cell.
6 7

5. ®
®
In-Spec® In-Spec® Amaranth
Patent Blue Standard Red Standard
Item # 8416 Item #8414
This popular product can be The In-Spec® Amaranth Red standard
customized to match a variety of was designed to complement the In-
wavelength and concentration levels. Spec® Patent Blue but can be used ex-
This reference standard is designed clusively for a variety of other processes.
for small volume instruments and When used together, the In-Spec® Patent
microplate readers but is equally Blue and Amaranth Red standards cover
adept on a traditional spectropho- a wider range of wavelengths that are
tometer. Typical applications include associated with applications in pharma-
analyses involving DNA, RNA, nu- ceutical and biotech laboratories. This
cleic acids, proteins, and enzymes. reference standard is similar to the In-
A commonly utilized wavelength for Spec® Patent Blue standard in that it can
this reference standard is 260nm. be used on microplate readers, traditional
spectrophotometers, and small path-
length instruments. Popular wavelengths
for this standard are 280nm and 320nm.
Sizes Available Sizes Available
60ml 60m l
125ml 125ml_
250ml 250ml
500ml 500ml
1l 1l
Sealed Cuvette Sealed Cuvette
8 Standards 9

6. ®
®
In-Spec® BCA Purple Standard In-Spec® Nano-Kit
Item # 8587 Item # 8476
The newest addition to the In-Spec® The In-Spec® Nano-Kit was awarded for the
product line came about due to innovation in solving the issue of validating short
demand to validate colorimeters and pathlength instruments without a toxic material.
spectrophotometers used in BCA pro- Prior to the kit’s introduction the only option was
tein assays. The wavelength of interest hazardous potassium dichromate solutions. The
for this application is 562nm. This In-Spec® Nano-Kit consists of our popular Patent
reference standard is equally customi- Blue and Amaranth Red reference standards with
zable as its Patent Blue and Amaranth standard data at 260nm, 280nm and 320nm.
Red counterparts. The data is certified with a 1mm pathlength cu-
vette eliminating the conversion from the 10mm
Sizes Available pathlength reading which is typical with many
60ml other standards.
125ml The reference standards are guaranteed for 1
50ml year from the date of shipment unlike many com-
500ml peting materials that are a one-time use product.
1l
Sealed Cuvette
10 Standards Nano-Kit 11

7. Wavelength Accuracy
Wavelength Accuracy Standards
The accuracy of your instrument’s wavelength scale is crucial
when obtaining absorbance readings on your sample. A slight
change of less than 1nm can cause an instrument’s read-
ings to shift leading to incorrect data. This puts the laboratory
at risk of passing a product that should have failed quality
control.
GFS Chemicals offers 3 different wave-
length reference filters that are used to
validate the wavelength scale. These
materials will add confidence to your
quality control process.
Listed below are the wavelength refer- Didymium Oxide Wavelength Standard Item # 8378
ence filters available with associated Peaks at: 298, 329, 355, 445, 469, 482, 512, 522, 576, 732, 740, 795, 802, 866nm
peaks. These materials are available in
sealed quartz cuvettes certified for 4
years or in HDPE bottles with a 1 year shelf life.
Recertification is available for the sealed quartz cuvettes.
Holmium Oxide Wavelength Standard Item # 8340 Samarium Oxide Wavelength Standard Item # 8379
Peaks at: 241, 250, 278, 287, 334, 345, 361, 386, 416, 451, 468, 485, 537, 641 nm Peaks at: 236, 267, 280, 290, 305, 318, 332, 345, 401, 416, 442, 464, 479 nm
12 13

8. Stray Light Filters Filters
Stray radiant light is energy reaching the detector that is not originating from the
Potassium Dichromate
Traditionally, potassium dichromate was used to check the
photometric accuracy of an instrument. GFS Chemicals Inc.
offers 5 different concentration levels. Using 3 or more of
these values will allow you to check the linearity of your instru-
instrument light source. This can come from a variety of sources and impacts ment. These standards come sealed in 10mm quartz cuvettes
the readings output by the instrument. Stray light can be a problem at any wave- or in bottles and are designed for use in the UV spectrum. The
length but is more prevalent in the UV range. sealed standards are certified for 1 year from shipment and
recertification service is available. Custom concentrations are
The use of a stray light filter can verify that an external light source is not effecting available. Please see the custom section on page 16.
the results. There are five commonly used materials to check the UV range of an
instrument. Each standard has a ‘cut-off’ wavelength and eliminates light trans- Catalog Numbers
mission below that level. Listed below are the standards offered and their associ- 20mg/L Item #8350
ated ‘cut-off’ wavelength. These are available in sealed quartz cuvettes certified for 40mg/L Item #8368
2 years or sealed HDPE bottles with a 1 year shelf life. Recertification is available. 60mg/L ltem #8369
80mg/L Item #8348
100mg/L Item #8372
Background
Solution Item # 8349
Potassium Iodide: 260nm
Item #: 8380
Lithium Carbonate: 227nm
Item #: 8382
Potassium Chloride: 200nm
Item #: 8384
Sodium Iodide: 260nm
Item #: 8381
Sodium Chloride: 205nm
Item #: 8383
14 15

9. ®
You may have a unique application Custom Standards
that requires verification of certain
absorbance values or wavelengths AMCO Clear®
that aren’t listed for our stock products. Turbidity Standards
Our experienced staff has the ability Amco Clear® is an EPA approved primary standard for cali-
to work with you to develop a refer- bration of turbidity sensors. It is available in a wide range of
ence standard that will validate your values. It has a guaranteed one year shelf life. The convenient
instrument for your application. submicron polymer suspensions are non-toxic primary
calibration standards approved by the US EPA for drinking
Please call technical support for water analysis for turbidity.
assistance. Amco Clear® is “The Clear Choice in Turbidity.”
In order to facilitate custom standard
development there are several key
pieces of data that are required.
With this information, technical
support will be able to quickly assess
your needs.
• Wavelength(s) of interest
• Pathlength
• Instrumentation make and model
• Desired or sample absorbance
range
Our problem-solving lab team is
available Monday through Friday
from 7:30 am to 4:00 pm EST.
AMCO Clear® Advantages:
• Safe, non-toxic and disposable
• Stable—does not settle out of suspension
• Requires no mixing or inverting
• Easy-to-Use/ No dilutions or preparations
• Accurate to 1% lot-to-lot
Custom
• Guaranteed one year shelf life from date of shipment
• Available in a wide range of values
• NIST Traceable
• Custom Standards Available
16 17

10. Application Notes
Specific Uv absorbance (SUva) for Total organic
Carbon (ToC) analysis
Standards for validation of Microplate Readers
& Short Pathlength Instruments
Specific UV Absorbance (SUVA) is an analysis of drinking water that With growing demand to for smaller sample analysis, microplate readers are becoming
uses UV absorbance at 254nm to identify dissolved organic carbon more common place in the biotech/pharmaceutical industry.
(DOC) levels. UV absorbance is easily determined using a spectropho- These instruments are typically used in performing various tests in relation to the analysis
tometer. Previous studies established a relationship between SUVA of DNA/ RNA, protein and enzymes. Many of the options that are used to verify the accu-
and the levels of humic substances that are removed during enhanced racy of these instruments are either expensive or limited in the scope that they can check.
coagulation and/or enhanced softening. Utilizing this relationship allows
for a cheap and easy method to determine whether enhanced coagula- GFS Chemicals has developed standards to validate the accuracy of instruments with equal
tion or softening is required based off of the exemption allowed in Stage to or less than 1mm pathlengths. The most popular application at this time is for users
1 D/DBPR and Stage 2 D/DBPR. Removing the extra disinfection from checking at 260 and 280nm.
the process can save a water district money when it comes to TOC
removal. This type of validation is where the In-Spec® Color standards work perfectly. The color
standards are designed to work with the built in software programs that manipulate the
Under Stage 1 D/DBPR, Standards Method 5910B was the only EPA data obtained in the plate and displays it as optical density. The color standards have many
approved method for SUVA determination. Stage 2 D/DBPR approved useful wavelengths, as exhibited on page 8 and 9. These can also be made in a wide range
EPA Method 415.3 (Revision 1.1, 2005) for TOC, DOC and SUVA of concentrations to better match the values you see in your daily testing.
determination. This one method not only combines all three tests, but
also provides increased quality control procedures to ensure testing ac- The color standards are available in a wide range of sizes and have a 1 year shelf life so
curacy, including calibration verification procedures for all instruments you can keep them on the shelf and ready to test your instrument when the need arises. All
utilized in the method. you need to do is pipet an aliquot into the wells on your plate and run them on the reader.
Once the data is obtained you then compare that to the values on the certificate of analysis
According to the EPA method, the verification of the spectrophotom- to verify that the instrument is performing as it should.
eter can be done with a commercially prepared check standard. This
GFS Chemicals Inc. also offers Holmium Oxide solution in individual bottles so that you
method specifically mentions the In-Spec® photometric standards,
may verify the wavelength scale on your scanning microplate reader. This provides a much
manufactured by GFS Chemicals, Inc, which were utilized by the
cheaper alternative to sealed plates with Holmium Oxide or a sealed quartz cuvette.
EPA to validate their spectrophotometer when developing 415.3. The
In-Spec® photometric standards are NIST traceable, non-toxic, have a
By utilizing both the color standards and the Holmium Oxide solution you will be able to say
one-year shelf life and require no preparation to use.
with confidence that the data you obtained on your microplate reader is accurate based off
NIST-traceable standards.
Data provided at 254nm for In-Spec UV Standards 1 through 30.
18 19

11. amaranth red
and its use in the Wine Industry
Notes
The In-Spec® Amaranth Red standard is ideal for use in the
TroubleShooting Guide
If your reference standards are not reading within the tolerance levels stated on
the certificate of analysis utilize the following guide prior to calling for techni-
cal support. Having the answers to the following questions will help expedite the
troubleshooting process.
wine industry for some common testing that is performed on
1) When was the last time the lamp was changed?
colorimeters or spectrophotometers. Wavelengths commonly
tested in wine samples for color intensity, hue, and total phe- a. GFS replaces the lamps in our instrument every 6 months, but with normal
nolic concentration analysis are 520nm, 420nm, & 280 nm. use, changing the lamps every 2 years should be sufficient.
b. Many older spectrophotometers do not have diagnostic capabilities to tell the
The color standards can be used to validate your instrument user when lamp energy is depleted or how many hours they have been in use.
so that you know that it is performing as it should or it can be
used as an inter-lab check so that you know your instrumen- 2) Are your cuvettes/cells clean, scratch-free and suitable for your range of use?
tation in one lab is matching that in another lab. Over time an a. All data provided has been obtained in a 10mm FUV quartz cuvette unless
instruments light source may dim and the values you obtain stated in the certificate of analysis.
will start to drift. b. Do not allow SDVB suspensions to dry on or inside your cells as it is difficult to
remove.
Knowing when this is occurring allows you to perform the
c. Disposable plastic cuvettes are generally not designed for the UV range unless
necessary maintenance before the issue impacts the quality
specified on the package.
of the wine that you are producing. Multiple concentrations
are available so that you may check a broad range of
absorbance values. 3) Was the supplied background solution* used to provide a baseline that
reads 0.0?
a. GFS has found that the “baseline” function alone may not provide a 0.000 ab-
sorbance when the background solution is scanned later. It may take a combina-
tion of “Auto zero” and “Baseline” over the range of interest.
b. See Certificate of Analysis for appropriate lot number of background solution.
4) How recently has your instrument had a full calibration that included wavelength
scale certification and stray light detection?
a. GFS has this performed twice yearly by the manufacturer
b. GFS provides NIST traceable wavelength and stray light check standards as well.
5) What spectral bandwidth are you using on your instrument?
a. All provided data is for a 1nm bandwidth unless otherwise specified.
b. Higher bandwidths often provide lower resolution and affect the values ob-
Guide
tained.
*Supplied with In-Spec UV and Visible standards
20 21

12. GFS Chemicals - Innovative Chemical Technology of the Society of Chemical Manufacturers & Affili-
for Innovative Applications ates (SOCMA) and the Ohio Chemistry Technology
Council, GFS is committed to conducting our
Analytical and Laboratory Chemists and technicians can connect to a single business with integrity and foresight. In addition,
Chemicals company with manufacturing resources in all key
areas of chemistry. Customers rely on us for our
GFS is committed to full implementation of the
SOCMA® ChemStewards® environmental, health,
problem solving ability. Our proven technologies, safety and security management system.
GFS manufactures over 6,000 organic and inorganic technical expertise, engineering excellence and
attention to detail allow us to work hand-in-hand Why Choose GFS Chemicals?
analytical reagent chemicals and specialties in a lean with our customers every step of the way – from
manufacturing operation. We have most chemicals startup to commercialization. GFS technology and • 80+ years of chemical manufacturing and chem-
products have kept us steps ahead of the indus- istry experience supporting process and
needed for laboratory, bench, analytical, quality control, try’s ever- changing chemical needs. commercial applications
materials characterization, scientific or research work. • Knowledgeable chemists and operators who
GFS Chemicals began when Professor G. Freder- have a complete understanding of the
ick Smith started producing perchloric acid in manufacturing process
Our ultra-high purity double-distilled Veritas® and ACS a small factory in Columbus, Ohio. Since then, • Scale-up flexibility from small sample sizes to
GFS has grown to become a leading producer of drums to metric tons, to ensure consistent quality
Superior™ brand acids and Watermark® brand reagents
specialty chemicals with performance-proven tech- from R&D to pilot to final production
are used throughout the world. Our AMCO Clear® nologies for laboratory, analytical, scientific, • International expertise shipping products to 85+
Water Analysis Division manufactures the highest water research and development, pilot scale production, countries means your products will arrive on time,
and manufacturing applications. meeting all regulatory compliance regulations
calibration standards available for water treatment plants • Technical support and resources every step of
around the globe. Project Customization the way
• Dedicated, responsive customer support
Our production environment gives us the flexibility personnel and points of contact
• Watermark® pyridine-free • Silver Compounds to offer you innovative solutions for all of your • Competitive pricing and reliable production
Karl fischer reagents • Reagents for Water chemical needs. GFS research and development lead times
can support you with the chemicals that your • U.S.-based quality manufacturing to ensure
• Veritas® hplC Solvents & Wastewater labs
application requires. Using our proven technology material availability, consistency and delivery
• HPLC/GC/UV-Vis Solvents • Organic Halogen Reagent – to customize your product, we create customer- • Experienced staff who understand the complexi-
• Reagent Grade Solvents OHR/Halogen Level Field specific item numbers and SKUs to streamline ties and dependencies of process chemistry
• Spectroscopy Products TestKits and safeguard your process. Our formalized and
repeatable ISO manufacturing ensures that you get Associations and Affiliations
• Primary Standard Salts • Polyethylene Glycol the same exact material every time. We package GFS Chemicals has been actively involved in the
• Normality Solutions (small quantities) according to your requirements in small packages scientific community for generations. Member-
through drums. ships include:
• Colors and Indicators • Conductivity Standards and If your application requires more stringent condi- • American Chemical Society
ph Buffers tions, we have experience with Good Manufac- • Society of Chemical Manufacturers and
• AA and ICP Standard Solutions turing Practice (GMP) processes. We are a key Associates (SOCMA)
supplier of critical materials for numerous Fortune • Tier II ChemSteward® Certified for Environmental
• Anion Standard Solutions
100, GMP manufacturers. We understand the Health and Safety
• Standard Solutions for needs of our GMP customers – including pharma- • American Water Works Association and Stan-
Trace Metal analysis ceutical manufacturers. dard Methods for the Examination of Water and
Wastewater
• ACS Reagents
Unmatched Product Quality • Water Environment Federation
• ASTM Type I Deionized water • Ohio Water Environment Association
GFS is an ISO 9001-2008 certified company. Our • ASTM International - Committees D19, E13,
quality control team guarantees product consis- E15 and E27
tency from lot-to-lot. We guarantee our products • American Council of Independent Laboratories-
to meet the quality standards that we publish, and Associate member
ship a Certificate of Analysis and a Material Safety • Ohio Manufacturers Association
Data Sheet (MSDS) with every order. • Chemical and Specialties Management Council
• Ohio Chemistry Technology Council
Environmental and Community Stewardship • U.S. Chamber of Commerce
• Ohio Chamber of Commerce
GFS was founded on a business philosophy of • National Federation of Independent Businesses
community stewardship and respect for our envi- • National Association of Manufacturers
ronmental resources. As a long-standing member • Tech Columbus
22 23

13. Team GFS
Are you sure your instrumentation is properly reading your
samples? Do your standard operating procedures require a vali-
dation standard? Over time small changes in your instrumenta-
tion can affect the data you obtain. Our reference standards
are a high quality, in-expensive way to add confidence to your
measurements.
GFS Chemicals Inc offers a full line of spectrophotometer
validation standards.
• Wavelength reference standards
• Photometric reference standards
• Stray Light reference standards
Contact information:
e-mail in-spec@gfschemicals.com
800-838-5567 extension 121 for technical assistance,
pricing and availability
(M-F 7:30 am to 4:00 pm Eastern Standard Time)
614-824-3299
740-881-5989 FAX
CHEMICALS
®
Visit www.in-spec.com for technical information
or Order online at www.gfschemicals.com