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markers in yeast yeast centrmere plasmid yeast episomal plasmid yeast replicating plasmid yeast artificial chromosome yeast integrating plasmid cup1 system gal system cloning saccharomyces cerevisiae schizosaccharomyces pombe pichia pastoris yeast hybridoma screening hat mdium monoclonal antibody hybridoma technology monoclonal antibody production & hybridization and procesing and editing & control rna splicing repressors transcription activators diagnostics drug discovery replacement of lab animals organ on a chip