1. The Streptococci With Emphasis on Streptococcus and Enterococcus www.freelivedoctor.com
2.
3.
4.
5.
6.
7.
8.
9.
10.
11. Lancefield Capillary Precipitation Antibody against a strep group antigen Strep Antigen Extract No Precipitate (Negative Test) Precipitate (Positive Test) Ag-Ab interface Ag-Ab interface Rabbit Anti- serum Rabbit Anti- serum Strep Antigen Extract www.freelivedoctor.com
12.
13. Preliminary Grouping – NOT on test Streptococcus Enterococcus Lactococcus Aerococcus Leuconostoc Pediococcus Gamella Broth ch tet + - + - + - - + + - - + + + Va S S b S S R R S PYR - a + - b + - - + LAP + + + - - + +/- NaCl - + +/- + +/- +/- - 45 o C +/- + - - - + - Broth=growth in broth; ch=chains; tet=tetrads; Va=vancomycin; PYR= pyrrolidonyl arylamidase; LAP=leucine aminopeptidase; NaCl=growth in 6.5% sodium chloride; Growth at 45 o C a Streptococcus pyogenes is PYR positive b Occasional exception Beta hemolysis +/- - b - - - - - www.freelivedoctor.com
14.
15.
16.
17.
18.
19.
20. Staph Streak For NVS Specimen streaked For isolated colonies Staph aureus culture Band streak NVS colonies “ Satelliting” the Staph streak www.freelivedoctor.com
25. Presumptive ID – NOT on test Organism GAS GBS Groups C,F,G Entero- coccus Group D Strep Viridans Pneumo- cocci Hemo- lysis a b , , , , Baci- tracin S R b R b NA NA NA NA SXT R R S NA NA NA NA CAMP - + - - - - - PYR + - - + - - - Bile Esculin - - - + + - b - Opto- chin NA NA NA R R R S NaCl - - b - + - - - NA=Not applicable; a Weak beta; b occasional exception www.freelivedoctor.com