This document provides information about a DNA extraction lab. It includes a list of materials needed for the lab such as a wooden stick, pipette, conical tubes, soap, water, ethanol, and plant samples. It instructs students to make a flow-gram and mix detergent every minute for 5 minutes. The purpose is to extract DNA and make general observations. It also includes introductory information about DNA structure including that DNA is made up of nucleotides with different nitrogenous bases that code for traits.

1. DNA Intro

2. Warm-ups!
O What is the chemical equation for
photosynthesis?
O What is the chemical equation for cellular
respiration?
O What are the steps of cellular respiration?
O Which step(s) do not require oxygen?
O Where does each step occur in the cell?
O *feel free to draw it out.
O What do you know about DNA and what do
you want to know?

3. A VIDEO
O http://www.youtube.com/watch?v=aQR_aaUqMPc
O http://www.youtube.com/watch?v=aIyaxgBkToU&feature=related

4. DNA Extraction Lab

5. Lab
Title: DNA Extraction Lab
Purpose: To extract DNA and make general
observations of DNA

6. Materials
O One wooden stick
O 1 pipette
O 1 50 ml conical tube
O 1 15ml conical tube
O Tube holder optional
O Soap
O 20mL water
O Waste beaker
O Ethanol
O Wheat Germ, Strawberry, Banana?

7. Make a Flow-gram

8. Mix the detergent once
every min. for 5 min

10. Intro to DNA

11. Zooming into DNA
http://www.thetech.org/genetics/zoomIn/
http://www.pbs.org/wgbh/nova/genome/dna
_flash.html

12. DNA stands for Deoxyribonucleic Acid
The structure of DNA is a double stranded
helix
DNA is important because it codes for all of
our traits.
http://www.biotechnologyonline.gov.au/popu
ps/int_dnazoom.html

13. DNA is a type of nucleic acid made up of
monomers called nucleotides

14. There are 3 parts to make a nucleotide

15. Warm up!!
O What are the 3 parts of a nucleotide?
O Which part makes each nucleotide different
O Work on your study guide until 8:50a
TO DO:
O Take out Photosynthesis and Cell Resp.
Annotations
O Take out Field Trip form with 5$
O Email Mackenzie at
mshultz@hightechhigh.org if you want study
help after school

16. Field Trip
O On Tuesday of Next Week!! Yay!!!
O You can bring a snack and water
O Wear comfortable clothing and shoes we
will be doing a lot of walking.

17. O The nitrogenous base is what makes each
type of nucleotide different

18. O The phosphate and the ribose sugar make
up the back bone of the DNA

19. There are 4 types of nucleotides that make up DNA
Adenine
Guanine
Cytosine
Thymine
http://www.yout
ube.com/watch
?v=HvJlnujmYc
g&feature=BFa
&list=PL9CDDA
BF9F37094D9
&lf=results_mai
n

20. Chargaff’s Ratio shows that the number of Adenine is
always equal to the number of Thymine and the
number of Guanine is always equal to the number of
Cysteine.
http://www.youtube.com/watch?v=HvJlnujmYcg&feat
ure=BFa&list=PL9CDDABF9F37094D9&lf=results_m
ain
http://www.youtube.com/watch?v=PDeaLxoL75M&fe
ature=BFa&list=PL9CDDABF9F37094D9&lf=results_
main

21. The nucleic acids of DNA pair up
O A pairs with T using 2 hydrogen bonds
O G pairs with C using 3 hydrogen bonds

22. O The strands of DNA are antiparallel.