Kristian HOLST-LAURSEN, de la Faculté des sciences, Section plantes & sol, de l'Université de Copenhague (Danemark), revient sur les méthodes rapides pour l'authentification des produits, en s'arrêtant sur le projet Européen "AuthenticFood".

1. Sûreté alimentaire
Quelles innovations pour
la maîtrise des contaminants
et l'authentification des produits
agricoles et alimentaires ?
> 13 & 14 novembre 2013
> Montpellier SupAgro INRA
www.rencontres-qualimediterranee.fr

2. Kristian HOLST
LAURSEN
Faculté des sciences,
Section plantes & sol,
Université de Copenhague
(Danemark)

3. Fast methods for authentication of organic based
food
- presentation of the "AuthenticFood" project
Laursen, Kristian Holst, Dr., University of Copenhagen

4. Organic versus conventional plant
production
Organic
Conventional
Pesticides
No
Yes
Synthetic fertilizers
No
Yes
Organic fertilizers
Yes
Yes
Fertilizer amount
Lower or similar
Higher or similar
Longer
Shorter
Lower or similar
Higher or similar
Higher
Lower
Crop rotations
Yields
Product price
Laursen, Kristian Holst, Dr., University of Copenhagen

5. Organic agricultural land in Europe
Source: www.fibl.org
Laursen, Kristian Holst, Dr., University of Copenhagen

6. Credibility in the organic market
- Certifiction does not protect consumers
Laursen, Kristian Holst, Dr., University of Copenhagen

7. Research objectives of
AuthenticFood
To develop and test a portfolio of analytical techniques for
authentication of organic plant foods
• Ionomics
• Stable isotopes
• Metabolomics
• Pesticide screening
To evaluate the techniques in corporation with European
inspection and certification bodies
• Ecocert - France
• BIOS - Italy
• DVFA - Denmark
Laursen, Kristian Holst, Dr., University of Copenhagen

8. AuthenticFood partners
KU‐Science
FOOD‐DTU
DVFA
Bioforsk
UHEL
FERA
LIAE
RIKILT
Unika
GL Centre
ICT
FP7 ERA‐NET project
16 partners
11 European countries
Project leader: Søren Husted
Period: 2011‐2014
Eurofins
Ecocert
AIAB
FEM‐IASMA
BIOS
Laursen, Kristian Holst, Dr., University of Copenhagen

9. AuthenticFood workpackages
WP1:
WP2:
WP3:
WP4:
WP5:
WP6:
WP7:
WP8:
Project coordination
Sample production and distribution
Multi-elemental analysis, 31P (NMR)
Stable isotopes, δ2H, δ13C, δ15N, δ18O, δ34S, nitrate
and sulfate isotopes, δ15N in amino acids
Metabolomics, phenolic content, volatile analysis, fatty
acids, sensory profiles
Pesticide residues
Chemometrics
Results dissemination
Laursen, Kristian Holst, Dr., University of Copenhagen

10. Biological cases
Experimental factors
•
•
•
•
•
•
Organic/conventional
Plant species
Plant variety
Growth season
Geography
Processing
Laursen, Kristian Holst, Dr., University of Copenhagen

11. AuthenticFood project status
Laursen, Kristian Holst, Dr., University of Copenhagen

12. Essential plant nutrients
The periodic table
H
He
Li
Be
B
C
N
O
F
Ne
Na Mg
Al
Si
P
S
Cl
Ar
Se
Br
Kr
K
Ca
Sc
Ti
V
Rb Sr
Y
Zr
Nb Mo
Cs Ba
La
Hf
Ta
Fr
Ac
Rf
Db Sg
Ra
Lanthanider
Actinider
Cr
W
Mn Fe
Co
Ni
Cu Zn
Ga Ge As
Tc
Ru
Rh
Pd Ag Cd In
Sn Sb Te
I
Xe
Re
Os
Ir
Pt
Pb Bi
At
Rn
Bh
Hs
Mt
Uun Uuu Uub
Gd
Au Hg Tl
Po
Ce Pr
Nd
Pm
Sm
Eu
Tb
Dy
Ho Er
Tm
Yb
Lu
Th
U
Np
Pu
Am Cm Bk
Cf
Es
Md
No
Lr
Non‐mineral
Pa
Macronutrient
Fm
Micronutrient
Laursen, Kristian Holst, Dr., University of Copenhagen

13. Basically all trace elements are found in plants
– The Plant Nano-nutrients
Mass scan 7Li – 238U
Essential Nutrients ( 11 B - 95 Mo)
Background subtracted spectrum
1e+10
1e+9
Mg
K
Se
Co
-1
-1
Ion intensity (counts s )
1e+8
Mn
1e+7
1e+6
1e+5
Mo
Cd
B
1e+4
1e+3
Ni reference line
1e+2
1e+1
1e+0
1e-1
0
50
100
150
200
250
Mass (m/z)
Laursen, Kristian Holst, Dr., University of Copenhagen

14. Multi-elemental plant tissue analysis
Laursen, Kristian Holst, Dr., University of Copenhagen

15. Stable isotope analysis
14N
15N
  15 Nsample  

 
14
  Nsample  
 1 1000
15
 Nsample    15

 Nair 

 


  14 N 
air 



99.63%
”light” isotope
Units o/oo
0.37%
”heavy” isotope
Laursen, Kristian Holst, Dr., University of Copenhagen

16. Multi-isotopic analysis can reveal the
fertilization history of plants
δ15N (bulk tissue)
How was I
fertilized?
Low δ15N
• Synthetic
• Legume
High δ15N
• Slurry
• Manure
• Compost
δ18O (nitrate)
Low δ18O
• Legume
High δ18O
• Synthetic
Laursen, Kristian Holst, Dr., University of Copenhagen

17. Conclusions
Metabolites
Stable isotopes
Trace elements
Compund‐specific
isotopes
Pesticide residues
Laursen, Kristian Holst, Dr., University of Copenhagen

18. Thank you for your attention!
www.coreorganic2.org/authenticfood
holst@life.ku.dk
Laursen, Kristian Holst, Dr., University of Copenhagen

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