Hplc

High Performance Liquid Chromatography (HPLC) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

What is HPLC? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Types of HPLC Separations (partial list) ,[object Object],[object Object],[object Object],[object Object],[object Object]

What does the analyst do? ,[object Object],[object Object],[object Object],[object Object],[object Object]

Columns and Stationary Phases. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Stationary Phases ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Optimization of Separations in HPLC ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

The Mobile Phase in HPLC... ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Typical HPLC Pump (runs to 4,000+ psi)

Polarity Index for Mobile Phases….. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Optimization of Mobile Phase Polarity… Changing the mobile phase composition alters the separation.

Isocratic versus Gradient Elution ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Injection in HPLC ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

LOAD (the sample loop) Inject (move the sample loop into the mobile phase flow)

Detection in HPLC ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Standard Absorbance Detector…. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Diode Array Detector (DAD) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Refractive Index Detector ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

ELSD (Evaporative Light Scattering Detector) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Hplc

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Hplc