Preethi, MD DVL, INDIA

1. Platelet rich plasma
Preethi chekuri

2. What is Platelet Rich Plasma?
It is an effective concentration of
multiple fundamental growth factors
(GF’s) by virtue of platelets alone
(stored as α-granules in platelets) and
plasma proteins
“REGENERATIVE”

3. • COCKTAIL of growth factors
• Degranulation of the pre-packed
GF’s in platelet
occurs upon “activation”
• Cacl2 and calcium gluconate
(1 in 10 dilution)

4. PRP preparation
Manual double spin method:
 Soft spin – 1200 rot/min for 10 min
 Hard spin – 2400 rot/min for 10 min

5. • Aim : to sequestrate platelets in high
concentrations, enough for achieving
therapeutic benefit and in a viable state at
the same time, so that they can actively
secrete their GF’s
• Precautions : aseptic condition
optimum temp regulation
anticoagulant

7. Indications for use
• Androgenic alopecia
• Alopecia areata
• Acne scars
• Skin rejuvenation
• Wound healing

8. Procedure
• A special dermaroller is
used to stimulate dermis of
scalp.
• The microneedling causes
microtrauma to dermis that
induces growth repair cells
into action.
• PRP is then applied
topically

9. Mechanism of action
Differentiation of hair follicle stem cells
bcl-2 : anti apoptotic – prolonged
survival of dermal papilla cells
Expression of FGF-7 : prolonged anagen
VEGF and PDGF - proangiogenic

10. Modes of usage
• PRP mesotherapy
• Used in conjunction with minoxidil daily
topical therapy
• Used as an adjunct to hair transplantation
(it is noted to increase the result)

11. Safety
• It is likely that hundreds of
thousands of humans have been
treated in clinical practice by now
• No major side effects reported
• AUTOLOGOUS – minimum risk
of reactivity compared to
exogenous compounds

12. In vitro research
• PRP increased in vitro proliferation
and differentiation of stem cells in the
hair follicle bulge area and has an
angiogenic role which makes it useful
as a hair growth modality
• Thrombin and CaCl2 increased GF
release in dose dependent manner
in-vitro

13. In vivo research
• Wide variability between patients in PRP
content
• Variability between PRP ‘resting’ and
‘activation’

14. Various
studies

15. Conclusion
PRP has been known and used in
dermatology, dentistry and sports medicine
The mechanism of action of GFs have been
studied and well established
So this technology should not be considered
experimental any more, although it can still
undergo further improvement in the furure

16. Thank you