2. 994 RAZEGHI, HADADI, MANSOR-KIAEI ET AL
high temperature, leukopenia, anemia, thrombocytopenia, Table 1. Clinical and Laboratory Manifestation of CMV in
atypical lymphocytosis, and deranged liver function tests.1 This Study
CMV infection can be identified by several methods, Frequency (%)
including pp-65 antigen detection on leukocytes, polymer-
Constitutional complaints 44 (67)
ase chain reaction (PCR) to detect CMV DNA in plasma Hematologic abnormalities
indicating active viral replication, viral culture, seroconver- Anemia 42 (64)
sion with the appearance of anti-CMV IgM antibodies; a Leukopenia 14 (21)
fourfold increase in preexisting anti-CMV IgG titers for Thrombocytopenia 31 (47)
qualitative detection of CMV.3 Fever 43 (65)
The clinical presentation, laboratory findings, and treat- GI effects
ment outcomes in kidney transplant recipients with CMV Abdominal pain 14 (21)
infection in our center have not been studied extensively. Diarrhea 13 (20)
Vomiting 10 (15)
We therefore conducted a retrospective analysis to examine
Pneumonitis
the incidence, clinical pattern of symptomatic CMV infec- Cough 21 (32)
tion, and factors affecting its presentation and treatment Sputum 9 (14)
outcomes in our renal patients. Dyspnea 15 (23)
Abnormal liver tests 14 (21)
METHODS Asymptomatic 13 (20)
We retrospectively reviewed the records of all renal transplant pa-
tients in our center from 2001 to 2006. Criteria for the diagnosis of
load and days needed for the patient to be cured was not
symptomatic CMV infection included the presence of symptoms and
significant (P .082).
signs or laboratory abnormalities plus detectable pp-65 antigen–
positive cells per 50,000 peripheral blood leukocytes. Although PCR Patients were treated with intravenous gancyclovir: oral
methods are emerging as the most clinically useful diagnostic method acyclovir was added in 12 (18%) patients. Pulse therapy was
with high sensitivity and specificity combined with rapid availability of done in 50(76%).
results. We used the pp-65 antigen to detect this disease because PCR Twenty-eight cases of recurrence and one death were
was not yet standardized in all laboratories in Iran and the pp-65 reported during this study. The estimated mean creatinine
antigen test is less expensive and more accessible. value was higher among the patients who experienced infec-
Depending on the organs or systems involved, the presentations tions (3.3) compared with the value estimated before diagnosis
were divided into (1) fever ( 38°C); (2) hematologic involvement (2.1) and after treatment (2.4). The difference in the mean
with either leukopenia (WBC 4300/L) or thrombocytopenia
creatinine before, at the time of CMV detection, and upon
(platelets 100,000/L) or anemia (significant decrease in hemoglo-
cure was statistically significant (P .001). Seventy- eight
bin); (3) a 25% rise in serum creatinine; (4) liver function
derangement (ALT or AST 40 IU/L); (4) pneumonitis; (5) percent of patients had a creatinine 2 mg/dL before the
gastrointestinal tract involvement; or (6) others. Patients fulfilling infection; creatinine remained 2 in only 26% when CMV
criteria for diagnosis of symptomatic CMV infection were enrolled was diagnosed, and in 60% after the treatment.
in this study.
For statistical analysis, the 2 test was used to compare differ- DISCUSSION
ences in proportions, and student’s t test was used to compare
It is well known that CMV infections are common among in
mean values.
renal transplant recipients.4 Symptomatic CMV infections
include a heterogeneous group of clinical features and
RESULTS
laboratory findings, ranging from mild infection to severe
There was evidence of CMV disease in 66 of 200 (33%) disease. The incidence of symptomatic CMV disease in our
patients, which was diagnosed at a median time of 9.7 center was 33%. The median time to diagnosis was 9.7
months (just after transplant to 10 years) posttransplanta- months posttransplant, 79% displayed symptomatic CMV
tion; 52 (79%) diagnoses were made within 6 months infections within the first 6 months. This period represents
posttransplantation. There were 42 males and 24 female the maximal immunosuppression for prevention and treat-
affected of overall mean age of 40 13 years (range, 14 to ment of acute rejection.
67 years). Recently, attention has been focused on the role of the
It is interesting to note that all donors and recipients quantitative CMV viral load as an accurate diagnostic test
enrolled in our study had previous exposure to CMV. After for CMV. Although in general, CMV viral load correlates
the diagnosis of CMV, the mean duration of pp65 antigen- with viral disease, CMV disease can occur in the setting of
emia was 13.8 days (range, 1 to 10 weeks). The clinical and a low viral load. In many studies, viral load was reported as
laboratory manifestations are shown in Table 1. All 27 an optimal parameter for monitoring responses to antiviral
patients who were referred due to symptoms related to therapy, whereas in our study there was no correlation
pneumonia had normal chest x-rays. between viral load and days needed for cure.3
A history of antilymphocyte globulin therapy was present CMV disease most commonly presents as a febrile illness,
in 29 (44%) of the patients. The correlation between viral but can present with only blood abnormalities (leukopenia,
3. KIDNEY RECIPIENTS WITH CMV INFECTION 995
thrombocytopenia, anemia, elevated liver enzymes) or with of CMV infection after treatment with gancyclovir is more
symptoms of solid organ involvement (pneumonia, retinitis, likely to occur if gancyclovir is discontinued while viral
colitis).2 The clinical presentation in this study was similar replication is ongoing. Therefore, it has been recommended
to other studies. Constitutional complaints were the most to continue gancyclovir until viral replication is no longer
common presentation, followed by leukopenia, anemia, evident.2
thrombocytopenia, fever, gastrointestinal tract involvement, As a result of the widespread use of antiviral prophylaxis
pneumonia, and abnormal liver function. However, pneu- and preemptive therapy, the incidence and severity of CMV
monitis, a severe complication, was rarely reported in other disease and its indirect effects are significantly reduced.
studies.1 Some of the clinical manifestations could be the However, there is the increasing recognition of gancyclovir-
result of CMV infection and the side effect of the drugs, but
resistant CMV infection. The overall incidence of gancyclovir-
as in our study they disappeared after the cure, suggesting
resistant CMV infection is 2.1%, which varies widely among
CMV infection to be the main cause.
transplant groups, with the highest incidence among recip-
Although mycophenolate mofetil has been shown in
ients of lung and combined kidney- pancreas transplants.
many studies to be the agent responsible for increasing the
incidence CMV infection,5 our study reported that the Gancyclovir-resistant CMV infection has been observed in
combination of mycophenolate mofetil, cyclosporine Ne- 0%, 0.3%, 1%, 9%, and 13% among liver, heart, kidney,
oral, and prednisolone used after the transplant in our lung, and combined pancreas– kidney transplant recipients,
center, has made the manifestation of CMV disease milder; respectively. In our study recurrence was reported in 23
in other words organ involvement seemed to occur less (42.4%) cases.1 There was no relation between recurrence
frequently after using this combination (data not shown). and viral load. The high incidence of recurrence in our
Prophylactic, deferred treatment, and preemptive treat- study may be due to the short-term treatment used for these
ment strategies have been described in renal transplanta- patients, whereas other studies have reported that 3 months
tion but not compared adequately to determine the best continued treatment with an oral agent is necessary to
strategy.5 In our center, prophylactic treatment included 2 prevent recurrence.
weeks of gancyclovir in patients who needed ALG or ATG According to our data, the patients who experience CMV
to prevent rejection or as induction therapy. disease have poorer renal function at the time of CMV
In the literature, patients were treated for CMV disease diagnosis. The difference in the mean creatinine between
at the discretion of the attending clinician with intravenous before, at the time of CMV detection, and when the patient
gancyclovir for a course of 10 to 14 days followed by 3 was cured was statistically significant. Thus, CMV infection
months of oral acyclovir with a temporary reduction in may influence renal function as creatinine was reduced to
immunosuppression. Oral valgancyclovir was also indicated its normal range after CMV treatment.
for a 3-month course in patients with less severe symptoms.5 CMV is also a significant underlying cause of morbidity
Our patients were treated with intravenous gancyclovir
and mortality in this setting. The impact of CMV on overall
until a week after disappearance of CMV antigen. A small
mortality was examined in a prospective, single-center study
proportion of patients (12%) required treatment using oral
of almost 500 patients who did not receive induction
acyclovir (400 to 800 mg four times a day) plus gancyclovir
therapy or CMV prophylaxis. Patients were monitored by
depending on the patient’s compliance and the severity of
weekly CMV pp-65 antigenemia for 100 days and followed
the disease. The reason for giving the additive drug was to
prevent recurrence. Valgancyclovir was not prescribed in for a median time of 66 months. Compared to those without
any patient because it is too expensive and not available for CMV, CMV disease was associated with a relative risk of
all. In addition, there was no relation between the type of overall mortality of 2.5, and surprisingly, asymptomatic
drug and the duration of treatment. CMV infection was associated with a relative risk of overall
Although a history of receiving antilymphocyte globulin mortality of 2.9.1 In our study, one patient died of cardiac
was present in 29 (43.9%) of the patients; there wasn’t a problems apparently unrelated to CMV infection. It is possi-
significant relation between receiving antilymphocyte glob- ble that the diagnosis and treatment of CMV infection was
ulin and viral load in these patients. In a study performed in done before organ involvement.
England, the difference in incidence of acute rejection In conclusion, symptomatic CMV infection was relatively
between patients experiencing no infection (7.1%) and common among our renal transplant population. Coming
those experiencing disease (35%) was clinically but not across any post transplant patient with constitutional symp-
statistically significant due to the small number of patients toms or an elevated serum creatinine, clinicians must always
in each group. One can speculate that the increased inci- think of CMV infection. This presumption along with the
dence of acute rejection among patients experiencing CMV efforts to develop more sensitive methods to detect early
disease is the cause of worse renal function at the first year CMV infections may be of value for surveillance strategies.
following transplantation. In our study, 50 of 66 patients Because of the high recurrence rate in these patients, there
needed pulse therapy due to evidence of possible rejection is a need for long-term treatment. Prophylaxis and treat-
by a raised creatinine in the remaining group, there was no ment of CMV infection may be enhanced with the intro-
evidence of rejection. Various studies have reported relapse duction of newer agents such as valgancyclovir.
4. 996 RAZEGHI, HADADI, MANSOR-KIAEI ET AL
REFERENCES 3. Nordoy I, Muller F, Nordal K, et al: Immunologic parameters
as predictive factors of cytomegalovirus disease in renal allograft
1. Yeung JS, Tong KL, Chan HWH: Clinical pattern, risk
recipients. J Infect dis 180:195, 1999
factors, and outcome of CMV infection in renal transplant recipi-
ents: Local experience. Transplant Proc 30:3144, 1998 4. Armstrong J, Evans A, Rao N, et al: Viral infections in renal
2. Geddes C, Church C, Collidge T, et al: Management of transplant recipients. Infect Immuni 14:970, 1976
cytomegalovirus infection by weekly surveillance after renal trans- 5. Waiser J, Budde K, Schreiber M, et al: Effectiveness of
plant: analysis of cost, rejection and renal function. Nephrol Dial deferred therapy with gancyclovir in renal allograft recipients with
Transplant 18:1891, 2003 cytomegalovirus disease. Transplant Proc 30:2083, 1998