Mission impossible

1. Night session
Pat Pataranutaporn (P.P.) JSTP #12
MISSION IMPOSSIBLE

2. Night session
Pat Pataranutaporn (P.P.) JSTP #12
MISSION IMPOSSIBLE

6. โครงงานของฉันและ
ประสบการณ์
Present งานต่าง
แดน

7. IMPOSSIBLEMISSION

8. IMPOSSIBLEMISSION

9. IMPOSSIBLE PROJECT

12. Family
JSTP

15. ??
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?
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? ?

18. “ Everyone has a question.
What's yours? “

19. “ Everyone has a question.
What's yours? “
“ Never be afraid to asked “

25. ZOMBI
E

27. Dr.Atichart Ketsuwan
and his reseach that
use mathematics to
study the history of
ancient temple
วัดอุ
โมง,เชียงให
ม่

32. NatureThe hidden treasure

41. Biomimicry

42. “ Never be afraid to asked “

43. IMPOSSIBLE

44. IMPOSSIBLE
IS NOTHING

46. IMPOSSIBLE

47. IMPOSSIBLE

48. IMPOSSIBLE
Right
solution

50. POLYSTYRENE

51. Styromelt™,http://www.polystyrenerecycle.com/

52. 100,000 Tons
Styromelt™,http://www.polystyrenerecycle.com/
of Polystyrene was produce in UK.

54. “Polystyrene Fact Sheet,” Foundation for Advancements
in Science and Education, Los Angeles, California.
"Ease of Disposal". Retrieved 2009-06-25

55. “Polystyrene Fact Sheet,” Foundation for Advancements
in Science and Education, Los Angeles, California.
"Ease of Disposal". Retrieved 2009-06-25
CARCINOGEN
Styrene Monomer
Carbon Monoxide

56. “Polystyrene Fact Sheet,” Foundation for Advancements
in Science and Education, Los Angeles, California.
"Ease of Disposal". Retrieved 2009-06-25
CARCINOGEN
Styrene Monomer
Carbon Monoxide

57. “Polystyrene Fact Sheet,” Foundation for Advancements
in Science and Education, Los Angeles, California.
"Ease of Disposal". Retrieved 2009-06-25
CARCINOGEN
Styrene Monomer
Carbon Monoxide
1%RECY
CLE

58. Information & Communication Technology Center,
Ministry of Energy, THAILAND
Natural degradation
>500 years

59. Information & Communication Technology Center,
Ministry of Energy, THAILAND
Natural degradation
>500 years

61. My inspiration

62. My inspiration

63. My inspiration

64. My inspiration

65. My inspiration

66. My inspiration

68. สิ่งเล็กๆที่เรียกว่า
แบคทีเรีย

69. สิ่งเล็กๆที่เรียกว่า
แบคทีเรีย

74. ??
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75. WHYPS foam
spend long time in
degradation ?

77. chemical
formula is (C8H8)n
Polymer of styrene
Hydrogen
Carbon

78. organic
compound
s
organic
compound
s
organic
compound
s
Naturally

79. POLYSTYRENE
organic
compound
s
organic
compound
s
organic
compound
s
Naturally

80. POLYSTYRENE
organic
compound
s
organic
compound
s
organic
compound
s
Naturally

81. POLYSTYRENE
organic
compound
s
organic
compound
s
organic
compound
s
Naturally

82. POLYSTYRENE
organic
compound
s
organic
compound
s
organic
compound
s
Naturally

83. HOW we isolate the
bacteria that able to
degrade foam ?

84. If bacteria can
degrade foam,
how it utilize ?

85. chemical
formula is (C8H8)n
Polymer of styrene
Hydrogen
Carbon

86. Bacteria nutritional requirements
‣ Energy source
‣ Carbon source
‣ Nitrogen source
‣ Minerals
‣ Water
‣ Growth factors

87. HOW we isolate the
bacteria that able to
degrade foam ?

90. POLYSTYRENE

91. Isolation of polystyrene foam biodegradable
bacteria from petrol contaminated soil
Researcher : Pat Pataranutaporn Advisor : Dr.Opas Tuntithagoon and Dr.Ampaithip Sukghom Co-Advisor : Rattanawan Inpang
Abstract 01
The purpose of this study was to isolate polystyrene foam
biodegradable bacteria from petrol contaminated soil. Mineral salt
medium with polystyrene foam as the only carbon source was used in
this experiment. After 4 months of incubation, surface of polystyrene
foam was observed compared with the control group. The result showed
that there were some traces of degradation on the surface of
polystyrene foam when compared to the control group which the same
source of soil was used as inoculum but had been sterilized before
inoculation. Twenty-four isolates of Gram-positive bacteria were
isolated from polystyrene foam degraded flasks, 19 isolates were bacilli
and 5 were cocci shape.
Introduction 02
Polystyrene foam is a type of plastic produced from styrene. It is a
lightweight, moisture-resistant material with exceptional insulation
properties. Products made from foamed polystyrene are nearly
ubiquitous, for example packing materials, insulation, and foam drink
cups. Polystyrene takes hundreds of years to decompose and is often
abundant as a form of pollution in the outdoor environment.
Methodology 03
Methodology. Soil samples were collected from sources that were
contaminated by petrol. One gram of contaminated soil sample was
inoculated into 100 ml of Mineral salt medium (K2HPO4, 2.0g; KH2PO4,
0.2g; (NH4)2SO4, 0.5g; MgSO4, 0.05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg;
MnCl2.4H2O, 0.003mg; CoCl2.6H2O, 0.012mg; CuCl2.2H2O, 0.001mg;
NiCl2.6H2O, 0.002mg; Na2MoO4.2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg,
H3BO3, 0.02mg with 1000 ml distilled water, pH 7.3) in 250 ml flask.
Small pieces of 0.1 g of polystyrene foam were added as carbon source.
Prior to use, polystyrene foam was soaked in 70% alcohol and washed
two times with distilled water and dried in desiccator for two days. In
the control group, 1 gram of sterilized petrol contaminated soil sample
was used. All experiment was carried out in triplicate. All flasks were
incubated in shaker at 150 rpm, 30oC for 2 months. After 2 months of
incubation 1 ml of cell suspension was inoculated into a new flask and
incubated in the same condition for 2 months. Surface of polystyrene
foam was observed under stereo zoom microscope and taken
photograph. Culture broth was diluted and spread onto glucose yeast
extract agar (4 gram of glucose and yeast extract in 1,000 ml of distilled
water, pH 7.3) and incubated at 30oC for two weeks, colonies of bacteria
were picked, purified and morphological studied by Gram-staining and
observed under microscope.
Result and discussion 04
The results showed that bacteria from petrol contaminated soil
could grow in mineral medium that polystyrene foam was the only
carbon source and 24 isolates of Gram-positive bacteria were isolated;
19 isolates were rod and 5 isolates were round shape. Furthermore,
some traces of degradation were found on the surface of polystyrene
foam from the experiment flasks rather than the control group. These
results indicated that there are polystyrene foam degradable bacteria
from petrol contaminated soil and It might use polystyrene foam as
carbon and energy sources. However, further investigation need to
be done for confirmation the results of these experiments and that is
our future study.
Acknowledgement: This project was supported by JSTP.
National Science and Technology Development Agency.
Experimental group Control group
Reference 05
Daniel Burd (2008) Plastic not fantastic.
Libraries (online). Available : http://www.science.uwaterloo.ca
Rod shape Positive gram
Round shape Positive gram
79.1%
(19 isolates )
20.9%
(5 isolates )
Types of bacteria that isolated from the experimental group
The surface of polystyrene foam

92. egraded flasks, 19 isolates were bacilli
02
of plastic produced from styrene. It is a
material with exceptional insulation
rom foamed polystyrene are nearly
g materials, insulation, and foam drink
ds of years to decompose and is often
n the outdoor environment.
gy 03
s were collected from sources that were
gram of contaminated soil sample was
ral salt medium (K2HPO4, 2.0g; KH2PO4,
05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg;
H2O, 0.012mg; CuCl2.2H2O, 0.001mg;
2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg,
distilled water, pH 7.3) in 250 ml flask.
ene foam were added as carbon source.
was soaked in 70% alcohol and washed
and dried in desiccator for two days. In
erilized petrol contaminated soil sample
carried out in triplicate. All flasks were
, 30oC for 2 months. After 2 months of
on was inoculated into a new flask and
be done for confirmation the results of these experiments and that is
our future study.
Experimental group Control group
Rod shape Positive gram
Round shape Positive gram
79.1%
(19 isolates )
20.9%
(5 isolates )
Types of bacteria that isolated from the experimental group
The surface of polystyrene foam

93. egraded flasks, 19 isolates were bacilli
02
of plastic produced from styrene. It is a
material with exceptional insulation
rom foamed polystyrene are nearly
g materials, insulation, and foam drink
ds of years to decompose and is often
n the outdoor environment.
gy 03
s were collected from sources that were
gram of contaminated soil sample was
ral salt medium (K2HPO4, 2.0g; KH2PO4,
05g; EDTA, 0.5mg; FeSO4.2H2O, 0.25mg;
H2O, 0.012mg; CuCl2.2H2O, 0.001mg;
2H2O, 0.003mg; ZnSO4.7H2O, 0.008mg,
distilled water, pH 7.3) in 250 ml flask.
ene foam were added as carbon source.
was soaked in 70% alcohol and washed
and dried in desiccator for two days. In
erilized petrol contaminated soil sample
carried out in triplicate. All flasks were
, 30oC for 2 months. After 2 months of
on was inoculated into a new flask and
be done for confirmation the results of these experiments and that is
our future study.
Experimental group Control group
Rod shape Positive gram
Round shape Positive gram
79.1%
(19 isolates )
20.9%
(5 isolates )
Types of bacteria that isolated from the experimental group
The surface of polystyrene foam
Experimental
group
control group

94. The results showed that bacteria from petrol contaminated soil could
grow in mineral medium that polystyrene foam was the only carbon
source and 24 isolates of Gram-positive bacteria were isolated; 19 isolates
were rod and 5 isolates were round shape.

95. Howto make sure that
bacteria that was isolate can
survive in our condition ?

96. Molecular biology technic

97. Molecular biology technic
time(week)

98. Molecular biology technic
time(week)
Microbe diversity

99. Molecular biology technic
time(week)
Microbe diversity
Dominant species

100. ControlTreatment

101. 100x 200x 500x
Polystyrene in Medium with Soil sample
regular polystyrene foam that didn’t use in experiment.

102. Magnification = 300X
Control Experimental group

104. WHYPS foam spend long time in degradation ?
HOW TO FIND THE MICROBE THAT ABLE TO DEGRADE PS FOAM ?
HOWwe isolate the bacteria that able to degrade foam ?
If bacteria can degrade foam, how it
utilize ?

105. ??
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106. Question

107. Question

108. No great discovery was ever
made without a bold guess
Isaac Newton

109. Start
Goal

110. Start
Goal
Why
Why
Why

111. Start
Goal
Why
Why
Why
Why
Why
Why

112. Start
Goal
Why
Why
Why
Why
Why
Why

114. osamu shimomura
Wins Nobel Prize in Chemistry 2008
for Discovery Of Green Fluorescent Protein

118. ??
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119. You have a chance
to answer your
own question .

120. “ Never be afraid to asked “

121. POSSIBLEIM

122. AKE POSSIBLEI M

123. THANK YOU

124. Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol(
(Current(advisor)(
Miss(Apinya(Boonkhum( Mrs.RaCanawan(Inpang(

125. Dr.Opas(Tun,thagoon( Dr.Ampai,p(Sookhom( Asst.Prof(Dr.Savaporn(Supaphol(
(Current(advisor)(
Miss(Apinya(Boonkhum( Mrs.RaCanawan(Inpang(
Advisor