Opportunity overview with OriGene Technologies. OriGene is looking to identify strategic collaboration partners for its full-length human proteins, validated monoclonal antibodies and "gene centric" tool box.

1. Developing Better Assays for
Every Human Protein
OriGene Technologies, Inc.
Mark E. Watson
Sr. Director Business Development
Phone: (240)994-6873
Email: mwatson@origene.com

2. Collaboration Opportunities with OriGene
• Strategic Licensing Agreements
• Supply agreement for cDNA clones, full-length human
proteins and validated monoclonal antibodies
-Purchase of full cDNA clone collection or subsets
-Human and Mouse ORF clones available
-Bulk full-length protein development
-Licensing of hybridoma cell lines (e.g. TrueMAB mAbs)
-Joint mAb and/or Hybridoma cell line development
-Custom multiplex assay development
• Collaboration agreements for antibody decoding and auto-
antibody studies using the proprietary protein lysate microarray
technology from OriGene
• Custom service projects leveraging gene synthesis
capabilities from Blue Heron Biotechnology, including codon &
antibody optimization

3. OriGene’s Key Capabilities
• Largest collection of cDNA clones in the world
• Protein production in human cells
– 12,000 transfected antigen lysates
– 5,000 purified proteins from HEK293Tcells
– Est.10,000 available by the end of 2011
– Heavy-labeled MRM/Mass spec. standards.
• High throughput mAb production/ validation
– Recently opened second facility to expand production to over 500 released and fully
validated mAbs/month
– Three fold increase in production capabilities by year end 2011
• Cytomyx tissue bank:
– >140,000 tissue samples
– High throughput IHC validation capabilities
• Synthetic Gene Production & Services
– Blue Heron Biotechnology Acquisition (Ab & Codon optimization)
• 10,000 Antigen Chip (Autoimmune & Cancer tests)
• Luminex multiplex assay development (Marligen acquisition)

4. Complete Gene/clone Resource
TrueORF/Gold True Proteins TrueMAB TruePlex
Human/mouse 5,000 human >1,800 mAbs
Expression vectors Custom purification IHC, IF, IP, FC, WB
Clones/genes Proteins Antibodies Assays
Any species Optimized Antibody engineering Multiplex Assays
Any sequences expression
Any variants

5. High Throughput Work Flow
Transient Transfection Cell Lysates Affinity
(MegaTran) Column
15,000 12,000 5,000
TrueORF Clones Over-expression Lysates Purified Protein
Largest offering of mammalian cell expressed human proteins

6. Highest Quality Standard
Not just sequence-verification
But also,
Expression Validation on 12,000 TrueORF clones
APO3C TP53 ENC1 CDH3 LRP1
TrueORF:RC206566 TrueORF:RC200003 TrueORF:RC201842 TrueORF:RC207346 TrueORF:RC218369
ORF size: 300 bp ORF size: 1182 bp ORF size: 1770 bp ORF size: 2490 bp ORF size: 13635 bp
Predicted MW: 11 kd Predicted MW: 53 kd Predicted MW: 65 kd Predicted MW: 91 kd Predicted MW: 504 kd

7. Transfected Over-Expression Lysates
• 12,000 TrueORF clone transfected HEK293T cell lysates
• Mammalian cell expressed full length, WB validated
• Delivered in modified RIPA buffer to preserve protein function
• Ideal tools for antibody validation, protein function studies, and
as assay standards

8. Over-expression Lysates
• OriGene over-expression lysates adopted in Antibody Atlas Program to
validate polyclonal antibodies (e.g. Atlas Antibodies).
• Improved validation success rate from 32% with regular cell lysates to 89% with over-
expression lysates.
• Data from Prof Mathias Uhlén, Program Director.

9. 10,000 Lysate Protein Arrays
E-Cadherin1
Enlarged image
Anti-DDK (Rabbit) Anti-E-Cadherin1 (mAb)
Using OriGene antigen microarray chip technology
to decode antibodies that were generated by whole cell immunization

10. Full Length Human Proteins
• Produced from TrueORF cDNA
clones
• 5,000 full length human proteins
• Expressed in HEK293 cells
• Optimal preservation of protein
structure, post-translational
modifications and functions

11. Human Protein Activity
mTOR Activity Assay
Homogeneous time-resolved
fluorescent (HTRF®) assay.
mTOR is a serine/threonine
protein kinase that regulates
cell growth, cell proliferation,
cell motility, cell survival,
protein synthesis, and
transcription. mTOR
belongs to the
phosphatidylinositol 3-
kinase-related kinase protein
family.
First commercial provider of active, full-length human mTOR protein

12. MS Collaboration with ISB
• Generate signature peptide sequences for all 5,000
human proteins
– Sequences will be in PeptideAtlas database
• Heavy-isotope labeled purified proteins
– 5,000 available now for SRM/MRM application
• Isotope labeled peptides
– Coming in 2011 for all 5,000 human protein

13. mAbs for Every Human Protein
 Dedicated mAb production center
- Capacity: +200 immunizations per month
 Authentic proteins as antigens
- Full length proteins made in human cells
 Extensive validation
- WB: 9 different cell lines
- IHC: 24 different tissues
- IF
- Flow cytometry
- Luminex
- Additional validation available
 Hybridoma cell lines for licensing
- High value targets available: ERCC1, LGR5,
HES1 & NOTCH1
Goal: To produce high quality mAbs
to every human proteins in 5-10
years

14. HTP Validation of mAbs
Anti-KRT WB with OriGene Anti-KRT WB with cell panel of 9 different cell lines
over expression lysate (For endogenous protein detection in cell line)
ERCC1 (1A3) ERCC1 (4F9)
IF data with A549 cells IF data with A549 cells
IHC data for human carcinoma liver tissue

15. Immunoassay Development
Quantitation of 6 Growth Factors in an 11-plex ELISA
30000
FGF -4
25000
Median Fluorescent Intensity
VEGF -D
EGF
20000
HGF
PlGF
15000
VEGF -A
10000
5000
0
1 10 100 1000 10000
Analyte Concentration (pg/ml)

16. Summary
 OriGene has developed the most comprehensive
genome wide product solutions available
 Leading developer of high quality reagents and
multiplex assays for every human protein
 OriGene is your perfect partner for companion
diagnostic and biomarker assay development
 Additional information available at:
www.origene.com