20 minutes ELISA test kit for the quantitative and sensitive detection of T2 and HT2 toxins in cereals and feed. For further informations, please visit Tecna website www.tecnalab.com.

1. T2
T2
Fast immunoassay for the detection of T2 and HT2 toxins in cereals and feeda
code MT 100 / MT101

2. Features
T2
Applications Standard curve example
Cereals, feed
Measuring range
0.025 - 1 ppm
Detection limit
Cereals, feed: 0.025 ppm
Assay time
20 minutes
Specificity
T2 toxin 100%
HT2 toxin 72%

3. Sample preparation
T2
Grinding, methanol extraction,
filtration, dilution
In details:
Weight 50 g of homogeneous and ground sample.
Add 10 g of NaCl.
Add 250 ml Methanol-water solution 70:30.
Shake 3 minutes.
Filter.
Dilute the extract 1:7 in water.

4. MAIZE validation
T2
Specificity and Sensitivity
10 blank maize samples were analysed.
The mean relative signal was 89±6%.
By subtracting 2 standard deviations, a cut off was set at
76%, corresponding to 0.025 ppm.
The same samples were spiked at 0.025 ppm T2 toxin.
One determination up to twenty turned to be negative.
The Limit of Quantification (LOQ) was set at 0.025 ppm.
The sensitivity turned to be 95%.
Summary
Maize cut-off: 0.025 ppm
Maize LOQ: 0.025 ppm

5. MAIZE validation
T2
Accuracy
A blank sample was spiked with T2 toxin in the range 0.1 -
1 ppm.
The mean recovery was 109±13%.
The graph on the right represents the linear
correlation between spiked toxin concentration and
results, including the results obtained at 0.025 ppm.
Trueness
Code T2 assigned value HT2 assigned value Sum and Celer T2 mean result
satisfactory range (n=5)
TC970 0.324 ppm 0.414 ppm 0.738 ppm 0.64±0.04 ppm
(0.508-0.960 ppm) Satisfactory
MTC999D 0.264 ppm 0.523 ppm 0.787 ppm 0.65±0.04 ppm
(0.479-1.074 ppm) Satisfactory

6. WHEAT validation
T2
Specificity and Sensitivity
10 blank wheat samples were analysed.
The mean relative signal was 84±5%.
By subtracting 2 standard deviations, a cut off was
set at 75%, corresponding to 0.026 ppm.
The same samples were spiked at 0.025 ppm T2
toxin.
All determinations were revealed as positive.
The Limit of Quantification (LOQ) was set at 0.025 Summary
ppm. Wheat cut-off: 0.026 ppm
The sensitivity turned to be 100%. Wheat LOQ: 0.025 ppm

7. OATS validation
T2
Specificity and Sensitivity
10 blank oats samples were analysed.
The mean relative signal was 79±8%.
By subtracting 2 standard deviations, a cut off was
set at 64%, corresponding to 0.030 ppm.
The same samples were spiked at 0.050 ppm T2
toxin.
All determinations were revealed as positive.
The Limit of Quantification (LOQ) was set at 0.050 Summary
ppm. Wheat cut-off: 0.030 ppm
The sensitivity turned to be 100%. Wheat LOQ: 0.050 ppm

8. FEED validation
T2
Specificity and Sensitivity
10 blank poultry feed samples were analysed.
The mean relative signal was 76±8%.
By subtracting 2 standard deviations, a cut off was set at
59%, corresponding to 0.047 ppm.
The same samples were spiked at 0.050 ppm T2 toxin.
All determinations were revealed as positive.
The Limit of Quantification (LOQ) was set at 0.050 ppm.
The sensitivity turned to be 100%.
Summary
10 blank samples of feed for pigs were implemented to study the
possible difference to the poultry ones. Their mean B/Bo was 77± Feed cut-off: 0.047 ppm
11%. This experiment confirmed the cut-off at 59% B/Bo. Feed LOQ: 0.050 ppm

9. Advantages
T2
sample preparation:
The common sample extraction enabels end-users to extract sample just once and
run the same extract within all Celer test kits:
Cereal sample
3 minutes methanol / water extraction
Feed sample
AFLA DON ZON FUMO T2
AFLA B1

10. Advantages
T2
Quickness:
Sample preparation: < 10 minutes
Assay time: 20 minutes
High HT2 cross-reactivity:
72% cross-reactivity to HT2
Good accuracy on T2-HT2 incurred samples
Double format available:
Code: MT100 - 96 determinations
Code MT101 - 48 determinations
PLUS: easy concentration calculation with downloadable spreadsheet

11. More
T2
Tecna’s port-folio of rapid and sensitive
tools for mycotoxin screening in cereals
B ZERO
fast ELISA test kits with no calibration curve for aflatoxin B1 and deoxynivalenol
fast ELISA test kits for total aflatoxins, aflatoxin B1, fumonisins, zearalenone, deoxynivalenol, T2/HT2 toxins
high sensitivity ELISA test kits for aflatoxins and ochratoxin A
EZ FLOW
New quantitative lateral flow for deoxynivalenol

12. Instruments and services
Aquaculture
Other foods
More
Allergens
Cereals
Honey
Dairy
Feed
Water
Meat
T2
TECNA CATALOGUE
Find what best fits your screening
necessities in different fields, for
different analytes.
Contact our team of experts for
technical support
Require our Performance Data
Sheets to see our in-house
validative results
Download our spreadsheet for a
fast and easy result calculation
Contacts
export@tecnalab.com
servizioclienti@tecnalab.com