For acceptance controls, for low throughput analysis, for outdoor, on-field screening, Smart Strip DON is Tecna NEW, quick, reliable lateral flow device for the qualitative and quantitative detection of deoxynivalenol (vomitoxin) in cereals. Easy to manage, easy and fast to perform, adaptable to different needs, Smart Strip DON is the right tool to analyse such mycotoxin! For further information visit Tecna website www.tecnalab.com or contact us at export@tecnalab.com!

1. Development of a quantitative lateral-flow
immunoassay for the detection of
deoxynivalenol in cereals

2. About deoxynivalenol
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•Fusarium spp. (mainly F. graminearum and F. culmorum)
WHO
•15 – 30 °C, water activity > 0.85
WHEN
•Cereals (mainly wheat, durum wheat, barley, maize and their derivatives)
WHERE
•Human gastroenteritis with nausea, diarrhea and vomiting.
•The toxicity in animals is reflected by feed refusal and growth retardation.
•All animal species are susceptible (pigs > mice > rats > poultry ≈ ruminants).
WHAT

3. Screening techniques
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Fastness–Easeofuse–suitabilityfor
acceptancecontrols
Sensitivity–Characterizationofanayltes–Skill
requirement

4. Lateral flow immunoassay
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Lateral flow immunoassays are tests based on a kind of immunochromatography. All
reagents are generally inside the device and this makes the test suitable to be performed
by almost everyone, everywhere.
As the sample is loaded on the sample pad, it migrates toward the conjugate pad, where it
dissolves the tracer, an anti-deoxynivalenol antibody colloidal gold conjugate. Thanks to capillarity,
the liquid flow migrates through the membrane, reaching the immunoreactive lines. On the Test (T)
line a conjugate is absorbed. This is a competitor to the free deoxynivalenol molecules eventually
present in the sample. The other line is the Control (C) line, where anti-species secondary antibodies
are adsorbed and which represent the positive control of the assay. In case the control line would
not be coloured, at the end of the assay time, the test is to be considered invalid.

5. Why?
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 Lateral flow devices are hence characterized by fastness and ease of
use.
 They don’t need many dedicated laboratory equipments.
 They are a good option for factories, plants and small industies with
low throughput analysis.
is now avaliable on the market for
the QUANTITATIVE detection of
deoxynivalenol (vomitoxin) in

6. The test kit
Kit insert
Dilution buffer
Pouches containing the strips
Barcode Businesscard
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7. Product presentation
 Assay principle: competitive lateral flow immunoassay for the
quantitative detection of deoxynivalenol
 Applications: cereals
 Measuring range: 500 - 12500 ppb
 Assay time: 10 minutes
 Sample preparation: water extraction, centrifugation, dilution
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8. Sample preparation
Weight 5 g of
ground sample
and add deionized
or distilled water
Shake thoroughly for
3 minutes
Allow sample to
settle down
Centrifuge the
upper phase 1
minute
Dilute with the
provided dilution
buffer
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9. Assay implementation
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Pipet the diluted
extract in the
sample well
Wait for 10 minutes
Put the developed cassettes in the reader

10. The reader
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LAB LFD READER
• Multiplex laboratory reader
• Ideal for testing more samples together
• Ideal for using different assays per time
• Up to 5 different assays can be read at a time

11. 11
 The quantification depends on the ratio of the colour intensity of the
TEST LINE (T) and the CONTROL LINE (C).
 The T/C value is interpolated by the reader onto a MASTER-
calibration curve that is uploaded with a barcode businesscard for
every kit batch.
 The Control line must be developed and homogeneous, otherwise
the test is invalid
 Both readers are able to verify wether the assay is compliant or not
Results achievement
Validation
 Specificity 100%: no compliant samples
are detected as positive
 Sensitivity 100%: all > 500 ppb samples are
detected as positive.

12. Remarks: why SMART STRIP DON
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# Plastic cassettes provide high manageability of the devices. A lot of room is available to
write the name or the code of each sample, thus ensuring its traceability.
# The sample preparation is dramatically easy: no solvent, incubator, vortex is necessary.
# There are no reagents to prepare and mix, no strip to cut or dry: the sample extract is
ready to be dropped on the sample pad. The reaction is incubated 10 minutes at room
temperature.
# The LAB LFD READER allows to save time when more samples are run together

13. Appendix
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 Other analytical methods can be chosen to have different
performances
 ELISAs are now available in different formats
1. Master-curve calibrated, the most cost effective layout, ideal for
those implementing a few samples
2. Fast, single replicate layout: 15 – 20 minutes assays
3. Double replicates, to have higher accuracy, reliability and
precision

14. Contacts
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