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05. Protein Sequencing

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How To Sequence A Protein W. Robert Midden Department of Chemistry Bowling Green State University
Protein Sequencing Preliminary Steps ,[object Object],[object Object],[object Object],[object Object],[object Object]
2-Mercaptoethanol ,[object Object],[object Object]
Preventing Reversal ,[object Object]
Perfomic Acid ,[object Object]
Reversal Prevented ,[object Object],[object Object]
Protein Sequencing Preliminary Steps ,[object Object],[object Object]
Determining Amino Acid Sequence ,[object Object],[object Object],[object Object],[object Object]
Determining Protein Sequence ,[object Object],[object Object],[object Object]
Determining Amino Acid Composition ,[object Object],[object Object],[object Object]
Determining the N-Terminal Amino Acid ,[object Object],[object Object],[object Object],[object Object],[object Object]
Determining the N-Terminal Amino Acid ,[object Object],[object Object],[object Object],[object Object],[object Object]
Determining the N-Terminal Amino Acid ,[object Object],[object Object],[object Object],[object Object],[object Object]
Determining the N-Terminal Amino Acid ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Determining the C-Terminal Amino Acid ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Peptide Fragments ,[object Object]
Why Use Fragments? ,[object Object],[object Object]
Why 2 Sets of Fragments? ,[object Object],[object Object],[object Object]
Protein Cleavage Reagents ,[object Object],[object Object]
Protein Cleavage Reagents ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Cyanogen Bromide ,[object Object],[object Object]
Trypsin & Chymotrypsin ,[object Object],[object Object],[object Object]
Clostripain ,[object Object],[object Object],[object Object],[object Object]
Staphyloccal Protease ,[object Object],[object Object],[object Object]
Endopeptidases ,[object Object],[object Object],[object Object],[object Object]
How are Peptide Fragments Separated? ,[object Object],[object Object]
Edman Degradation ,[object Object],[object Object],[object Object]
Edman Degradation ,[object Object],[object Object],[object Object],[object Object],[object Object]
Edman Degradation
Disulfide Bridges ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Mass Spectroscopy ,[object Object],[object Object],[object Object],[object Object],[object Object]
Protein Sequencing by DNA Sequencing ,[object Object],[object Object]
Sequence Databases ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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05. Protein Sequencing

  • 1. How To Sequence A Protein W. Robert Midden Department of Chemistry Bowling Green State University
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