Bill Faloon presented an age reversal update on a webinar hosted by the Coalition for Radical Life Extension on January 23rd, 2021. These are his presentation slides.

1. These slides available for review at: www.age-reversal.net
Age Reversal Update
William Faloon
January 23, 2021

2. 98-Year Average Lifespan?
25% Lifespan Extension
It May Be Possible for Normal Aged People to
Benefit from these Kinds of Single-Gene Crispr Edit
If this Crispr-Treatment Works Similar in Normal Aged Humans
Crispr-Treated Normal AgedMouse
Science Translational Medicine. 06 Jan 2021:Vol. 13, Issue 575, eabd2655. DOI: 10.1126/scitranslmed.abd2655

3. Crispr-Treated Progeroid Mouse
17 Months
>160 Years?
7.1 Months
Combination Crispr-Treated Human
Untreated Progeroid Mouse
https://www.nature.com/articles/s41586-020-03086-7 https://doi.org/10.1073/pnas.1910073116
Extrapolation Assuming
Normal Aged People
Can Have Multi-Gene Edits
via Crispr

4. December 30, 2020
https://fortune.com/2020/12/30/anti-aging-research-health-care-spending-biden/
“Cracking the code of biological aging
could solve America’s health care crisis”
“The most exciting opportunity
for such an improvement in
health productivity is to
understand and address the
biology of aging.”

5. Dec. 30, 2020
 Therapeutic PlasmaExchange ---ConboyLabatUCBerkeley
 Thymic Regeneration---Greg FahyatInterveneImmune
 Reverseextra-cellular protein stiffening (glycation)
Age-Reversal Interventions Highlighted:
https://www.sciencedirect.com/science/article/pii/S1568163720302324
https://fortune.com/2020/12/30/anti-aging-research-health-care-spending-biden/

6. “Reversal of epigenetic aging and immunosenescent trends in humans.” Aging Cell; Sept 2019
Human Age Reversal Demonstrated in 2019
1) Human growth hormone
2) DHEA
3) Metformin
Study conducted by Dr. Greg Fahy in collaboration
with researchers from Stanford University and
UCLA consisted of individualized doses of:
Study subjects also provided with daily
vitamin D3 and zinc.

7. https:// www.dailymail.co.uk/health/article-7435427/
Oct.22,2019
Turning Back Time!
“Aging is REVERSED in men using a
cocktail of growth hormones and
diabetes drugs in study that saw test
group shed 2.5 biological years.”

8. Intervene Immune’s TRIIM-X Study:
Thymus Regeneration Trial Enrolling
Now 100 healthy 50–80-year-olds targeting
reversal of immune system decline.
Study volunteers can enroll remotely
from across the U.S.
Intensive immune system and
epigenetic age monitoring.
Personalized drug cocktail reversed
epigenetic age by >2.5 years in healthy
men in TRIIM trial at Stanford.
Find out more:
www.ClinicalTrials.gov NCT04375657
GrimAge

9. Dec. 30, 2020
https://fortune.com/2020/12/30/anti-aging-research-health-care-spending-biden/
Mainstream Recognition of
The Need to Reverse Human Aging
“Age is the biggest risk factor for cancer,
cardiovasculardisease,andneurodegeneration.
Withouttreatmentsto slowor reverseaspects
of biologicalaging,anagingpopulationmeans
we areinfor ahealthcarecosttsunami.
With such treatments, Americans would
experiencemorehealthy,productiveyearsoflife.”

10. “…why not launch an
Operation Warp Speed
for biological aging?”
CONCLUSION
https://fortune.com/2020/12/30/anti-aging-research-health-care-spending-biden/

11. Our Groups Recognized the Urgency
Sought Validated Methods to
Reverse Aging in People
Manhattan Beach Summit
November 2009

12. Huge Advances in 12 Years
No Way to Reverse Aging
Manhattan Beach Summit Attendees
November 2009
Many Potential Methods
2009 2021

13. December 2, 2015
George Church, Ph.D.
A Cure for Aging by Year 2030?
https://www.washingtonpost.com/news/achenblog/wp/2015/12/02/professor-george-church-says-he-can-reverse-the-aging-process/

14. https://www.nature.com/articles/s41586-020-03086-7
January 6, 2021

15. https://www.wsj.com/articles/crispr-gene-editing-treatment-
could-point-way-to-fix-for-deadly-aging-disease-11609950054
“… mice with progeria that got the
gene-editing treatment lived more than
twice as long as the untreated mice.”
Crispr Gene-Editing Treatment
Jan 7, 2021|

16. https://www.wsj.com/articles/crispr-gene-editing-treatment-
could-point-way-to-fix-for-deadly-aging-disease-11609950054
“Scientists using a version of Crispr gene editing succeeded in
significantly extending the lives of mice with progeria…
These animals lived about two-and-½ times longer
than mice with progeria that weren’t given the treatment.
The edited mice, which got the injection three or 14 days after birth, lived
for nearly 1.5 years—roughly the start of old age in healthy mice.”
“Rapid Aging Disease Targeted by Gene Editing”
Jan. 7, 2021

17. Progeria is a rare, progressive genetic disorder that causes children to age rapidly,
starting in their first two years of life.
Children with progeria generally appear normal at birth. During the first year, signs and
symptoms, such as slow growth and hair loss, begin to appear.
Heart problems or strokes are the eventual cause of death in most children with progeria.
The average life expectancy for a child with progeria is about 13 years. Some with the
disease may die younger and others may live longer, even up to 20 years.
There's no cure for progeria, but ongoing research shows some promise for treatment.
Progeria
https://www.mayoclinic.org/diseases-conditions/progeria/symptoms-causes/syc-20356038
Rapid Accelerated Aging

18. Hutchinson-Gilford
syndrome
(Progeria)
Photo:
https://www.britannica.com/science/progeria
1 https://rarediseases.org/rare-
diseases/hutchinson-gilford-progeria/
1. Rare genetic disorder (1 in ≈20 million)1
2. Genetic mutation produces progerin, a highly toxic protein
3. Symptoms parallel many aspects of normal human aging.
4. Very limited treatment options
5. Humans age-to-death in 13-20 years

19. Crispr Gene-Editing Treatment
An untreated progeria mouse at 7.5
months of age, with a thin, grayish
coat, pronounced spinal curvature,
and low activity levels. Untreated
progeria mice have an average
lifespan of 7.1 months.
Three treated progeria mice at 11
months of age, with healthy
coats, no apparent spinal
curvature, and normal activity
levels. The treated progeria mice
had an average lifespan of 17
months.
CRISPR-Edited
Heathy Mice
Unedited
Rapid-Aged
Mouse
https://www.wsj.com/articles/crispr-gene-editing-treatment-could-point-way-to-fix-for-deadly-aging-disease-11609950054

20. Crispr Therapy Corrects Pathologic Mutations in DNA Genes
Healthy Longevity Conferred by Replacing Progeroid DNA
base pair T and A with Normal C and G DNA base pair
https://www.wsj.com/articles/crispr-gene-editing-treatment-could-point-way-to-fix-for-deadly-aging-disease-11609950054

21. 1. A ‘base editor’ was designed to home
in on this mutation and correct it to the
normal sequence. The molecule was put
into a special inactivated virus.
How this Gene
Editing Therapy
Extended Life
https://www.wsj.com/articles/crispr-gene-editing-treatment-could-point-way-to-fix-for-deadly-aging-disease-11609950054

22. https://www.wsj.com/articles/crispr-gene-editing-treatment-could-point-way-to-fix-for-deadly-aging-disease-11609950054
Crispr Gene-Editing Treatment
“Could Point Way to Fix for Deadly Aging Disease”
Jan. 7, 2021

23. Integrated Circuit Chip
Miles of Programmed Code
+ =
Reliable Smart Phones

24. OptimisticExtrapolationofCrisprMouseStudytoHumanAging
 Untreated progeria mice average lifespan: 7.1 months1
 Crispr-treated progeria mice average lifespan: 17 months1
 Untreated normal human lifespan: 78 years
 Combination Crispr-treated human lifespans: >160 years
Human age reversal will require combination Crispr-like gene therapies2
1.https://www.nature.com/articles/s41586-020-03086-7
2. PNAS November 19, 2019 116 (47) 23505-23511; https://doi.org/10.1073/pnas.1910073116

25. Untreated Progeroid Mouse
Crispr-Treated Progeroid Mouse
7.1 months
17 months
Combination Crispr-Treated Human
> 160 Years?
Extrapolation Assuming Normal Aged People
Can Have Multi-Gene Edits via Crispr
https://www.nature.com/articles/s41586-020-03086-7
https://doi.org/10.1073/pnas.1910073116

26. Results from single dose combination gene therapy:
Noah Davidsohn, Matthew Pezone, Andyna Vernet, Amanda Graveline, Daniel Oliver, Shimyn Slomovic, Sukanya
Punthambaker, Xiaoming Sun, Ronglih Liao, Joseph V. Bonventre, and View ORCID Profile George M. Church
PNAS November 19, 2019 116 (47) 23505-23511; https://doi.org/10.1073/pnas.1910073116
November 19, 2019
1)58%increasedfunctionafterheartfailure
2)38%reductioninvasculardiseasemarker
3) 75% reduction in kidney atrophy
4)Complete reversal ofobesity &diabetes

27. 1) 25% extended lifespan in normal aged mice.1
2) Improved overall appearance and grip strength.1
“A genome-wide CRISPR-based screen identifies
KAT7 as a driver of cellular senescence”
January 6, 2021
1. https://stm.sciencemag.org/content/13/575/eabd2655/tab-pdf
2. https://www.genome.gov/10001345/importance-of-mouse-genome
Mice and humans share virtually the same set of genes though in slightly different forms.2
KAT7 gene is significant driver of cell senescence. Targeting KAT7 using CRISPR enabled:

28. Crispr-Treated Normal Aged Mouse
25% Lifespan Extension
If this Crispr-Treatment Works Similar in Normal Aged Humans
98-Year Average Lifespan?
Extrapolation Assuming Normal
Aged People Benefit from this Single-Gene Crispr Edit
https://stm.sciencemag.org/content/13/575/eabd2655/tab-pdf

29. Maximum natural lifespan of
bowhead whales is 268 years.
DNA Genes of Bowhead Whale Sequenced
For Human Crispr Replication Studies
https://cosmosmagazine.com/biology/life-clock-ticking
Supported by grants from Life Extension® and the Methuselah Foundation.
Crispr-edited human DNA will
soon be studied using bowhead
whale genome.

30. Do You Want Un-Edited Genes? or Precisely Edited Genes?
Rough Draft Manuscript Finalized “Edited” Publication
or

31. newscientist.com/article/mg23931920-600-how-a-positive-mind-really-can-create-a-healthier-body
“George Church: The maverick
geneticist now wants to reverse aging”

32. DEC. 8, 2019
“A Harvard geneticist's goal: to
protect humans from viruses,
genetic diseases, and aging”
“George Church's lab at Harvard Medical School is
working to make humans immune to all viruses, eliminate
genetic diseases and reverse the aging process…”

33. December 2, 2015
George Church, Ph.D.
A Cure for Aging by Year 2030?
https://www.washingtonpost.com/news/achenblog/wp/2015/12/02/professor-george-church-says-he-can-reverse-the-aging-process/

34. Live 25% Longer Now!

35. “In the multi-variable model,
high adherence to the
Mediterranean diet was
associated with 25% lower
risk of death as compared
with the lowest category.”
https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/mediterranean-diet-and-mortality-in-
the-elderly-a-prospective-cohort-study-and-a-metaanalysis/F2D6B083AA187849477112DB77820521
“Mediterranean diet and mortality
in the elderly: a prospective cohort
study and a meta-analysis”
Published online by Cambridge University Press: 30 August 2018

36. Mediterranean diet and longevity
A. Trichopoulou* and E. Vasilopoulou
University of Athens Medical School, Department of Hygiene and Epidemiology, Mikras Asias 75, 115 27 Athens, Greece
British Journal of Nutrition (2000), 84, Suppl. 2, S205±S209
The conclusion of these studies is that a diet that adheres to the principles of the
traditional Mediterranean one is associated with longer survival. The Greek
version of the Mediterranean diet is dominated by the consumption of olive oil
and by high consumption of vegetables and fruits. Antioxidants represent a
common element in these foods and an antioxidant action provides a plausible
explanation for the apparent benefits. Wild edible greens frequently eaten in rural
Greece in the form of salads and pies contain very high quantities of flavonoids
considerably higher than those found in red wine or black tea. While there is no
direct evidence that these antioxidants are central to the benefits of the
Mediterranean Diet, indirect evidence from epidemiological data and the
increasing understanding of their mechanisms of action suggest that antioxidants
may play a major role.
https://www.researchgate.net/profile/Effie_Vasilopoulou/publication/12090631_Mediterranean_diet_and_longevity/links/00b4951e39aed3d079000000.pdf

37. May 2008 Sept 2016
April 2015
Longevity Benefits of Mediterranean Diet

38. https://www.mayoclinic.org/healthy-lifestyle/nutrition-and-healthy-eating/in-depth/mediterranean-diet/art-20047801
Mediterranean Diet Healthy Foods
More fruits, vegetables, and whole grains, and less dairy & meat than typical Western diet.

39. Foods to Reduce/Avoid on Mediterranean Diet
Re
 Red meat
 Overcooked meats
 Processed red meat (hot dogs, bacon)
 Butter
 Cured ham (nitrite/nitrite preserved meats)
 Most frozen meals
 Soda, desserts, candy
 Most processed foods
 Sugars
 Refined grains (white bread/white grain pasta)
 Excess salt
https://www.foodnetwork.com/healthy/articles/what-foods-are-not-allowed-on-mediterranean-diet

40. https://www.health.harvard.edu/staying-healthy/eating-more-ultra-processed-foods-may-shorten-life-span
May 2019
After nine years, the researchers found a direct
statistical connection between higher intake of
ultra-processed food and a higher risk of early
death from all causes, especially cancers and
cardiovascular disease.
Eating more ultra-processed foods may shorten life span

41. https://www.health.harvard.edu/staying-healthy/eating-more-ultra-processed-foods-may-shorten-life-span
May 2019
 Ready-to-eat foods
 Microwaveable foods
 Bread, breakfast cereals
 Instant noodles
 Chicken or fish nuggets
 Chocolate bars, candies, chips
 Artificially sweetened beverages
Ultra-processed foods defined as:

42. Foods like instant noodles and soups, breakfast cereals and
chicken nuggets were associated with an earlier death.
Feb. 29, 2019
The authors suggest that high-
temperature processing may form
contaminants, that additives may
be carcinogenic, and that the
packaging of prepared foods can
lead to contamination.
https://www.nytimes.com/2019/02/12/well/eat/eating-processed-foods-longevity-death-mortality.html
Avoid High Temperature Cooked Foods

43. New evidence links ultra-processed
foods with a range of health risks May 29, 2019
Two large European studies
find positive associations
between consumption of
highly processed ('ultra-
processed') foods and risk
of cardiovascular disease
and death.
https://www.sciencedaily.com/releases/2019/05/190529221040.htm

44. Small Increases in Ultra-processed Food Intake
Markedly Increases Incidence of Stroke and Heart Attack
May 29, 2019
Results showed that an absolute
10% increase in the proportion of
ultra-processed food in the diet was
associated with significantly higher
rates of overall cardiovascular
disease, coronary heart disease, and
cerebrovascular disease (increase of
12%, 13%, and 11% respectively).
https://www.sciencedaily.com/releases/2019/05/190529221040.htm

45. LowerDiseaseRiskWithUnprocessed
Or Minimally Processed Foods
May 29, 2019
In contrast,the researchers
found a significant
association between
unprocessed or minimally
processed foods and lower
risks of all reported diseases.
https://www.sciencedaily.com/releases/2019/05/190529221040.htm

46. "Ultra-processed" foods linked to
heart disease, early death
May 30, 2019
In the second study — of nearly 20,000 Spanish
adults — ultra-processed foods were linked to a
shorter life span:
Those with the highest intake were 62% more likely
to die over two decades, compared to those with the
lowest intake.
https://www.cbsnews.com/news/ultra-processed-foods-linked-to-heart-disease-early-death/

47. January 7, 2021
Adherence to the Mediterranean diet and grade group
progression in localized prostate cancer: An active
surveillance cohort
https://acsjournals.onlinelibrary.wiley.com/doi/10.1002/cncr.33182
Each one-unit improved adherence to the Mediterranean diet was
associated with a 12% lower trend of aggressive (high Gleason score)
prostate cancer detrimental progression.
A four-unit improved Mediterranean diet score would equate to a 48%
associated lower trend of prostate cancer progression.

48. Longitudinal Study Update
Vitality in Aging Research Group has shifted into the digital realm.
With a new electronic consenting and secure online data capturing platform.
To enroll, select "Enroll Now" hyperlink on Vitality in Aging website to create:
VIA Longitudinal Study of Aging Account Profile
Annual requirements for the study:
1) Age Management Blood Test Panel with Omega-3 Index Kit ($495)
2) Online Health Questionnaires
The VIA Longitudinal Study of Aging will collect data for 10 years.
To apply or enroll in VIA studies: VitalityInAging.org/

49. “Plasma dilution improves cognition and
attenuates neuroinflammation in old mice”
“Explaining the mechanism of the positive effects of
neutral blood exchange on the brain, our
comparative proteomics analysis demonstrated that
dilution of old blood plasma yields an increase in the
determinants of brain maintenance and repair in
mice and in people.
These findings confirm the paradigm of rejuvenation
through dilution of age-elevated systemic factors
and extrapolate it to brain health and function.”
https://link.springer.com/article/10.1007/s11357-020-00297-8
November 15, 2020

50. Sheldon G. Adelson, a cabdriver’s son who built
the world’s largest empire of casinos and resort
hotels in Las Vegas, Macau, Singapore and other
gambling meccas.
The cause was complications of non-Hodgkin’s
lymphoma.
Donated to many charities including some that
supported medical research.
Who missed the longevity boat?
Sheldon Adelson
(1933 - 2021)
Expiration date: Jan. 11, 2021
net worth > $35.8 billion https://www.palmerfuneralhomes.com/obituary/Arthur-Decio

51. Human Age Reversal Project
$1,018,600
Donations for
Vitality in Aging
Intervention Trial:
Goal: Statistically Significant +
Real World Age Reversal

52. Fund Research with Tax-Deductible Donations
Human Age Reversal Project
Make checks payable to:
Human Age Reversal Project
300 NE 20th St. #409
Boca Raton, FL 33431
Or donate online:
age-reversal.net/donate

53. Copyright ©Life Extension®. Confidential and proprietary.
How I Fund Age Reversal Research
Supplement Sales Support Rejuvenation Projects

54. Unparalleled Track Record
of Biomedical Innovation
Established 1977

55. Crispr-Treated Normal Aged Mouse
25% Lifespan Extension
If this Crispr-Treatment Works Similar in Normal Aged Humans
98-Year Average Lifespan?
Extrapolation Assuming Normal
Aged People Benefit from this Single-Gene Crispr Edit
https://stm.sciencemag.org/content/13/575/eabd2655/tab-pdf

56. Untreated Progeroid Mouse
Crispr-Treated Progeroid Mouse
7.1 months
17 months
Combination Crispr-Treated Human
> 160 Years?
Extrapolation Assuming Normal Aged People
Can Have Multi- Gene Edits via Crispr
https://www.nature.com/articles/s41586-020-03086-7
https://doi.org/10.1073/pnas.1910073116

57. Manhattan Beach Summit Attendees
November 2009

58. 98-Year Average Lifespan?
Extrapolation Assuming Normal Aged People
Can Have Multi-Gene Edits via Crispr
25% Lifespan Extension
If this Crispr-Treatment Works Similar in Normal Aged Humans
Crispr-Treated Normal AgedMouse
Science Translational Medicine. 06 Jan 2021:Vol. 13, Issue 575, eabd2655. DOI: 10.1126/scitranslmed.abd2655

59. > 160 Years?
17 Months
If this Crispr-Treatment Works Similar in Normal Aged Humans
Crispr-Treated ProgeriodMouse
Science Translational Medicine. 06 Jan 2021:Vol. 13, Issue 575, eabd2655. DOI: 10.1126/scitranslmed.abd2655
Untreated ProgeroidMouse
7.1 Months

60. age-reversal.net
This entire presentation available at: