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ŁOWCY MIKROBÓW 2008 w poszukiwaniu teorii wszystkiego Wojciech Marlicz  Klinika Gastroenterologii PAM, Szczecin Kongres PTG-E Gdańsk 13-15 czerwiec 2008
„ The dependence of the intestinal microbes on the food makes it possible to adopt measures to modify the flora in our bodies and to replace the harmful microbes by useful microbes.” 1. Mietchnikoff E.  The prolongation of life. Optimistic studies.  London: Butterworth Heinemann, 1907  2. Pasteur L.  et al.: Summary report of the experiments conducted at Pouilly-le-Fort, near Melun,    on the anthrax vaccination, 1881.
1. Guamer F.  et al.: Mechanism of Disease: the hygiene hypothesis revisited. Nature Clinical Practice Gastroenterology & Hepatology. 2006; 3, 275-284 2. Weiss ST. : Eat dirt – the hygiene hypothesis and allergic diseases. N Engl J Med. 2002,19; 47(12):930-1
Jama ustna Żołądek Dwunastnica Jelito czcze Jelito kręte Jelito grube Odbyt Lactobacilli  1-100/g Lactobacilli  1x10 2 /g Streptococci  1x10 4 /g Lactobacilli Streptococci Enterobacteria Bifidobacteria Yeasts 1x10 5 /g 1x10 6  /g Bifidobacteria Lactobacilli Streptococci Bacteroides Enterobacteria 1x10 5  /g 1x10 8  /g Bacteroides Eubacteria Bifidobacteria Anaerobic cocci Clostridia Lactobacilli Enterobacteria Streptococci 1x10 10  /g 1x10 11  /g
Gill SR et al.:  Metagenomics analysis  of the human distal gut microbiome.  Science, 2006; 312 (5728):1355-1359
 
Madara J. N Engl J Med 2004;351:1685-1686 Tickling Toll-like Receptors (TLRs)
Guarnerl F  et al.   (2006)  Mechanisms of Disease: the hygiene hypothesis revisited Nat Clin Pract Gastroenterol Hepatol   3:  275 – 284  doi:10.1038/ncpgasthep0471 Decision-making in the adaptive immune system is modulated by innate costimulatory signals
Wilson I.:  Top-down versus bottom-up rediscovering physiology via Systems biology?  Molecular Systems Biology, 2007, May, 3, 113
Ley RE  et al.: Microbial ecology: human gut microbes associated with obesity.  Nature 2006; 444 (7122): 1022-1023  Turnbaugh PJ et al.:  An obesity-associated gut microbiome with increased capacity for energy harvest.  Nature, 2006, 444; 21; 6-9
Siebler J.  et al.: The gut-liver-axis: Endotoxemia, inflammation, insulin resistance and NASH.  J Hepatology, 2008; 48, 1030-1034 Thalheimer U et al.:  Infection, coagulation and variceal bleeding in cirrhosis.  GUT, 2005; 54; 556-563
Rescigno M  et al.: The Pathogenic Role of Intestinal Flora in IBD  and Colon Cancer.  Current Drug Targets, 2008, 9, 395-403
Scanian PD  et al.:  Culture-independent analysis of the gut microbiota in colorectal cancer  and polyposis.  Environmental Microbiology. 2008, 10 (3), 789-798 Hope ME  et al.: Sporadic colorectal cancer – role of the commensal microbiota.  FEMS Microbiology Letters. 2005; 244; 1-7
Amaral FA  et al.: Commensal microbiota is fundamental  for the development of inflammatory pain.  PNAS, 2008, Vol 105, 6; 2193 Tjellstrom B  et al.: Gut microflora Associated  Characteristics in Children with Celiac Disease.  Am J Gastroenterology, 2005; 100: 2784-2788
 
 
Bjerrum   JT  et al.  (2008)  Technology Insight: metabonomics in gastroenterology—basic principles and potential clinical applications Nat Clin Pract Gastroenterol Hepatol  doi:10.1038/ncpgasthep1125   The relationship between the different 'omics' and their interaction via defined biologic processes
Metabonomika - to nowa dziedzina zajmująca  się całościową oceną dynamicznych  zmian metabolicznych zachodzących  w organizmach żywych.  Marchesi JR et al.:  Rapid and noninvasive metabonomic characterization of inflammatory bowel disease.  J Proteome Res 2007; 6: 546-551 Saric J et al.:  Species variation in the fecal metabolome gives  insight into differential gastrointestinal function.  J Proteome Res 2008; 7: 352-360
O’Hara AM et al.:  The gut flora as a forgotten organ. EMBO 2006, vol 7, 7, 688
Mikroflora jelitowa + + + + Farmabiotyki Jia W., Zhao L., Nicholson JK.:  Gut microbiota: a potential new territory  for drug targeting.  Nature Review, Vol 7; Feb 2008 Inne mikroorganizmy Prebiotyki Antybiotyki Probiotyki TURBO Leki stabilizujące błonę komórkową (leki izopatycze?) Fragmenty DNA LPS
 
 
Corr S.C. et al.:  Bacteriocin production as a mechanism  for the antiinfective activity of  Lactobacillus salivarius  UCC188.  PNAS, 2007, vol 104; 18, 2617-2621 Farmabiotyki TURBO Probiotyki
Sleator and Hill:  Designer probiotics:  a potential therapeutic for  Clostridium difficile ?  J Med Microbiol. 2008; 57: 793-794   TURBO Probiotyki Lactococcus lactis   Thy 12 Steidler et al.: Treatment of murine colitis by Lactococcus lactis Secreting interleukin-10.  Science 2000; 289 (5483):1352-5 Vandenbroucke K et al.:  Active delivery of trefoil factors  by genetically modified Lactoccus lactis prevents and heals  acute colitis in mice.  Gastroenterology 204; 127(2):502-13 Braat H et al.:  A phase I trial with transgenic bacteria expressing  interleukin-10 in Crohn’s disease.  Clin Gastroenterol Hepatol  2006; 4(6):754-9
 

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ŁOWCY MIKROBÓW 2008 w poszukiwaniu teorii wszystkiego

  • 1. ŁOWCY MIKROBÓW 2008 w poszukiwaniu teorii wszystkiego Wojciech Marlicz Klinika Gastroenterologii PAM, Szczecin Kongres PTG-E Gdańsk 13-15 czerwiec 2008
  • 2. „ The dependence of the intestinal microbes on the food makes it possible to adopt measures to modify the flora in our bodies and to replace the harmful microbes by useful microbes.” 1. Mietchnikoff E. The prolongation of life. Optimistic studies. London: Butterworth Heinemann, 1907 2. Pasteur L. et al.: Summary report of the experiments conducted at Pouilly-le-Fort, near Melun, on the anthrax vaccination, 1881.
  • 3. 1. Guamer F. et al.: Mechanism of Disease: the hygiene hypothesis revisited. Nature Clinical Practice Gastroenterology & Hepatology. 2006; 3, 275-284 2. Weiss ST. : Eat dirt – the hygiene hypothesis and allergic diseases. N Engl J Med. 2002,19; 47(12):930-1
  • 4. Jama ustna Żołądek Dwunastnica Jelito czcze Jelito kręte Jelito grube Odbyt Lactobacilli 1-100/g Lactobacilli 1x10 2 /g Streptococci 1x10 4 /g Lactobacilli Streptococci Enterobacteria Bifidobacteria Yeasts 1x10 5 /g 1x10 6 /g Bifidobacteria Lactobacilli Streptococci Bacteroides Enterobacteria 1x10 5 /g 1x10 8 /g Bacteroides Eubacteria Bifidobacteria Anaerobic cocci Clostridia Lactobacilli Enterobacteria Streptococci 1x10 10 /g 1x10 11 /g
  • 5. Gill SR et al.: Metagenomics analysis of the human distal gut microbiome. Science, 2006; 312 (5728):1355-1359
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  • 7. Madara J. N Engl J Med 2004;351:1685-1686 Tickling Toll-like Receptors (TLRs)
  • 8. Guarnerl F et al. (2006) Mechanisms of Disease: the hygiene hypothesis revisited Nat Clin Pract Gastroenterol Hepatol 3: 275 – 284 doi:10.1038/ncpgasthep0471 Decision-making in the adaptive immune system is modulated by innate costimulatory signals
  • 9. Wilson I.: Top-down versus bottom-up rediscovering physiology via Systems biology? Molecular Systems Biology, 2007, May, 3, 113
  • 10. Ley RE et al.: Microbial ecology: human gut microbes associated with obesity. Nature 2006; 444 (7122): 1022-1023 Turnbaugh PJ et al.: An obesity-associated gut microbiome with increased capacity for energy harvest. Nature, 2006, 444; 21; 6-9
  • 11. Siebler J. et al.: The gut-liver-axis: Endotoxemia, inflammation, insulin resistance and NASH. J Hepatology, 2008; 48, 1030-1034 Thalheimer U et al.: Infection, coagulation and variceal bleeding in cirrhosis. GUT, 2005; 54; 556-563
  • 12. Rescigno M et al.: The Pathogenic Role of Intestinal Flora in IBD and Colon Cancer. Current Drug Targets, 2008, 9, 395-403
  • 13. Scanian PD et al.: Culture-independent analysis of the gut microbiota in colorectal cancer and polyposis. Environmental Microbiology. 2008, 10 (3), 789-798 Hope ME et al.: Sporadic colorectal cancer – role of the commensal microbiota. FEMS Microbiology Letters. 2005; 244; 1-7
  • 14. Amaral FA et al.: Commensal microbiota is fundamental for the development of inflammatory pain. PNAS, 2008, Vol 105, 6; 2193 Tjellstrom B et al.: Gut microflora Associated Characteristics in Children with Celiac Disease. Am J Gastroenterology, 2005; 100: 2784-2788
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  • 17. Bjerrum JT et al. (2008) Technology Insight: metabonomics in gastroenterology—basic principles and potential clinical applications Nat Clin Pract Gastroenterol Hepatol doi:10.1038/ncpgasthep1125   The relationship between the different 'omics' and their interaction via defined biologic processes
  • 18. Metabonomika - to nowa dziedzina zajmująca się całościową oceną dynamicznych zmian metabolicznych zachodzących w organizmach żywych. Marchesi JR et al.: Rapid and noninvasive metabonomic characterization of inflammatory bowel disease. J Proteome Res 2007; 6: 546-551 Saric J et al.: Species variation in the fecal metabolome gives insight into differential gastrointestinal function. J Proteome Res 2008; 7: 352-360
  • 19. O’Hara AM et al.: The gut flora as a forgotten organ. EMBO 2006, vol 7, 7, 688
  • 20. Mikroflora jelitowa + + + + Farmabiotyki Jia W., Zhao L., Nicholson JK.: Gut microbiota: a potential new territory for drug targeting. Nature Review, Vol 7; Feb 2008 Inne mikroorganizmy Prebiotyki Antybiotyki Probiotyki TURBO Leki stabilizujące błonę komórkową (leki izopatycze?) Fragmenty DNA LPS
  • 21.  
  • 22.  
  • 23. Corr S.C. et al.: Bacteriocin production as a mechanism for the antiinfective activity of Lactobacillus salivarius UCC188. PNAS, 2007, vol 104; 18, 2617-2621 Farmabiotyki TURBO Probiotyki
  • 24. Sleator and Hill: Designer probiotics: a potential therapeutic for Clostridium difficile ? J Med Microbiol. 2008; 57: 793-794 TURBO Probiotyki Lactococcus lactis Thy 12 Steidler et al.: Treatment of murine colitis by Lactococcus lactis Secreting interleukin-10. Science 2000; 289 (5483):1352-5 Vandenbroucke K et al.: Active delivery of trefoil factors by genetically modified Lactoccus lactis prevents and heals acute colitis in mice. Gastroenterology 204; 127(2):502-13 Braat H et al.: A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn’s disease. Clin Gastroenterol Hepatol 2006; 4(6):754-9
  • 25.  

Notas do Editor

  1. Figure 1. Tickling Toll-like Receptors (TLRs). Commensal bacteria secrete TLR ligands such as lipopolysaccharide and lipoteichoic acid, which interact in the normal intestine with a population of surface TLRs. The resultant basal signaling, which is normally ongoing, enhances the ability of the epithelial surface to withstand injury while also priming the surface for enhanced repair responses. Because many types of cells on the surface of the intestine -- epithelial cells, lymphocytes between the epithelial cells, subepithelial mesenchymal cells, macrophages, and dendritic cells -- express TLRs, it is not known whether this critical homeostatic mechanism is maintained by the cell populations that also respond by means of TLRs when the surface is breached. Either the disruption of TLR signaling or the removal of TLR ligands compromises the ability of the intestinal surface to withstand insult and effect repair.