SlideShare uma empresa Scribd logo

Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

•
•
L
lleung

D145: Genomics and Proteomics

Tecnologia
1 de 28
Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells Michael Boutros, Amy A. Kiger, Susan Armknecht, Kim Kerr, Marc Hild, Britta Koch, Stefan A. Haas, Heidelberg Fly Array Consortium, Renato Paro, Norbert Perrimon (2004) Presenters: Lian Lim Luigi Leung
History of RNAi ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
RNAi and the Nobel Prize ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],Technicalities
Purpose of Study ,[object Object],[object Object],[object Object],[object Object]
Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Z-score Transformation ,[object Object],[object Object],[object Object],Cheadle, C., Vawter, M., Freed, W., Becker, K. “Analysis of Microarray Data Using Z Score Transformation.” J Mol Diagn 5.2 (2003 May): 73-81
Methods/Results - Observe cells ,[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Methods/Results - Observe cells ,[object Object],[object Object]
Methods/Results - Observe cells (z-score >3)
Methods/Results - Observe cells ,[object Object],[object Object]
Methods/Results - Further Analysis ,[object Object],[object Object],[object Object],[object Object],[object Object]
Method - Flow Cytometry
Methods/Results - Further Analysis ,[object Object]
Method - TUNEL ,[object Object]
Methods/Results - Further Analysis ,[object Object]
Methods - Rescue ,[object Object]
Methods/Results - Further Analysis ,[object Object]
Recap ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Importance of the Study ,[object Object],[object Object],[object Object]
Importance of RNAi ,[object Object],[object Object],[object Object],[object Object]
Further Reading RNAi – A powerful tool to unravel hepatitis C virus–host interactions within the infectious life cycle Joachim Lupberger, Laurent Brino, Thomas F. Baumert Focusing on RISC assembly in mammalian cells Junmei Hong, Na Wei, Alistair Chalk, Jue Wang, Yutong Song, Fan Yi, Ren-Ping Qiao, Erik L.L. Sonnhammer, Claes Wahlestedt, Zicai Liang, Quan Du Analysis of Microarray Data Using Z Score Transformation Cheadle, C., Vawter, M., Freed, W., Becker, K.
More on RNAi ,[object Object],[object Object],[object Object],[object Object]
Questions/Comments

Recomendados

Genomics lecture 3
Genomics lecture 3Genomics lecture 3
Genomics lecture 3iainj88
 
Gene knockoff
Gene knockoffGene knockoff
Gene knockofftanvi Sharma
 
Sandesh pawar master seminar
Sandesh pawar master seminarSandesh pawar master seminar
Sandesh pawar master seminarsnehaljikamade
 
RNA interference Activity in Glassy-winged Sharpshooter Cells and Whole Insec...
RNA interference Activity in Glassy-winged Sharpshooter Cells and Whole Insec...RNA interference Activity in Glassy-winged Sharpshooter Cells and Whole Insec...
RNA interference Activity in Glassy-winged Sharpshooter Cells and Whole Insec...huyng
 
RNA Interference (RNAi) and RNA Induced Gene Silencing
RNA Interference (RNAi) and RNA Induced Gene Silencing RNA Interference (RNAi) and RNA Induced Gene Silencing
RNA Interference (RNAi) and RNA Induced Gene Silencing Vigneshwaran Vellingiri
 
Antisense rna experiments lead to the discovery of
Antisense rna experiments lead to the discovery ofAntisense rna experiments lead to the discovery of
Antisense rna experiments lead to the discovery ofBruno Mmassy
 
RNA interference (RNAi)
RNA interference (RNAi) RNA interference (RNAi)
RNA interference (RNAi) KK CHANDEL
 
(Rn ai)
(Rn ai)(Rn ai)
(Rn ai)Ghazwa Shawash
 

Recomendados

Genomics lecture 3
Genomics lecture 3Genomics lecture 3
Genomics lecture 3iainj88
 
Gene knockoff
Gene knockoffGene knockoff
Gene knockofftanvi Sharma
 
Sandesh pawar master seminar
Sandesh pawar master seminarSandesh pawar master seminar
Sandesh pawar master seminarsnehaljikamade
 
RNA interference Activity in Glassy-winged Sharpshooter Cells and Whole Insec...
RNA interference Activity in Glassy-winged Sharpshooter Cells and Whole Insec...RNA interference Activity in Glassy-winged Sharpshooter Cells and Whole Insec...
RNA interference Activity in Glassy-winged Sharpshooter Cells and Whole Insec...huyng
 
RNA Interference (RNAi) and RNA Induced Gene Silencing
RNA Interference (RNAi) and RNA Induced Gene Silencing RNA Interference (RNAi) and RNA Induced Gene Silencing
RNA Interference (RNAi) and RNA Induced Gene Silencing Vigneshwaran Vellingiri
 
Antisense rna experiments lead to the discovery of
Antisense rna experiments lead to the discovery ofAntisense rna experiments lead to the discovery of
Antisense rna experiments lead to the discovery ofBruno Mmassy
 
RNA interference (RNAi)
RNA interference (RNAi) RNA interference (RNAi)
RNA interference (RNAi) KK CHANDEL
 
(Rn ai)
(Rn ai)(Rn ai)
(Rn ai)Ghazwa Shawash
 
Principle and method of RNAi-Creative Biogene
Principle and method of RNAi-Creative BiogenePrinciple and method of RNAi-Creative Biogene
Principle and method of RNAi-Creative BiogeneDonglin Bao
 
RNA interference (RNAi):A therapeutic strategy for aquaculture
RNA interference (RNAi):A therapeutic strategy for aquacultureRNA interference (RNAi):A therapeutic strategy for aquaculture
RNA interference (RNAi):A therapeutic strategy for aquacultureidrish123
 
Rna interference in insect pest management
Rna interference in insect pest managementRna interference in insect pest management
Rna interference in insect pest managementamoldchokhat
 
Rna interference
Rna interferenceRna interference
Rna interferenceNITIN KUMAR SINGH
 
Genetic engineering
Genetic engineeringGenetic engineering
Genetic engineeringIlika Kaushik
 
Creative biogene-The Extended Applications of RNAi
Creative biogene-The Extended Applications of RNAiCreative biogene-The Extended Applications of RNAi
Creative biogene-The Extended Applications of RNAicreative biogene
 
RNA interference in Biology and medicine
RNA interference in Biology and medicine RNA interference in Biology and medicine
RNA interference in Biology and medicine shahid chamran university of Ahvaz
 
Transgene silencing
Transgene silencingTransgene silencing
Transgene silencingpooranachithra flowry
 
Rna interferences in crop improvement
Rna interferences in crop improvementRna interferences in crop improvement
Rna interferences in crop improvementTAJNE SACHIN
 
Rn ai @ sid
Rn ai @ sidRn ai @ sid
Rn ai @ sidsidjena70
 
RNA interference
RNA interferenceRNA interference
RNA interferenceRazavi Nader
 
RNA interference (RNAi)
RNA interference (RNAi)RNA interference (RNAi)
RNA interference (RNAi)Haris Saddique
 
RNA INTERFERENCE TECHNOLOGY
RNA INTERFERENCE TECHNOLOGYRNA INTERFERENCE TECHNOLOGY
RNA INTERFERENCE TECHNOLOGYAshok2404
 
Rna silencing
Rna silencingRna silencing
Rna silencingUrooj Sabar
 
RNA interference (RNAi) in plants
RNA interference (RNAi) in plantsRNA interference (RNAi) in plants
RNA interference (RNAi) in plantsMohammadMosaddequlIs
 
Role of antisense RNAi in gene silencing in crop
Role of antisense RNAi in gene silencing in cropRole of antisense RNAi in gene silencing in crop
Role of antisense RNAi in gene silencing in cropKheta Ram Tak
 
Antisense genes in plants and their applications in crop improvement
Antisense genes in plants and their applications in crop improvementAntisense genes in plants and their applications in crop improvement
Antisense genes in plants and their applications in crop improvementAmritaGiri5
 
Rna interference
Rna interferenceRna interference
Rna interferencepranatee behera
 
gene silencing-for disease managment
gene silencing-for disease managmentgene silencing-for disease managment
gene silencing-for disease managmentBalaji Rathod
 
Gene Silencing (RNA interference )
Gene Silencing (RNA interference )Gene Silencing (RNA interference )
Gene Silencing (RNA interference )Mesele Tilahun
 
2013programmed cell dealth r
2013programmed cell dealth r2013programmed cell dealth r
2013programmed cell dealth rtamoohy
 
Apoptosis
ApoptosisApoptosis
ApoptosisImran Ahmad
 

Mais conteúdo relacionado

Mais procurados

Principle and method of RNAi-Creative Biogene
Principle and method of RNAi-Creative BiogenePrinciple and method of RNAi-Creative Biogene
Principle and method of RNAi-Creative BiogeneDonglin Bao
 
RNA interference (RNAi):A therapeutic strategy for aquaculture
RNA interference (RNAi):A therapeutic strategy for aquacultureRNA interference (RNAi):A therapeutic strategy for aquaculture
RNA interference (RNAi):A therapeutic strategy for aquacultureidrish123
 
Rna interference in insect pest management
Rna interference in insect pest managementRna interference in insect pest management
Rna interference in insect pest managementamoldchokhat
 
Creative biogene-The Extended Applications of RNAi
Creative biogene-The Extended Applications of RNAiCreative biogene-The Extended Applications of RNAi
Creative biogene-The Extended Applications of RNAicreative biogene
 
Rna interferences in crop improvement
Rna interferences in crop improvementRna interferences in crop improvement
Rna interferences in crop improvementTAJNE SACHIN
 
RNA interference (RNAi)
RNA interference (RNAi)RNA interference (RNAi)
RNA interference (RNAi)Haris Saddique
 
RNA INTERFERENCE TECHNOLOGY
RNA INTERFERENCE TECHNOLOGYRNA INTERFERENCE TECHNOLOGY
RNA INTERFERENCE TECHNOLOGYAshok2404
 
Role of antisense RNAi in gene silencing in crop
Role of antisense RNAi in gene silencing in cropRole of antisense RNAi in gene silencing in crop
Role of antisense RNAi in gene silencing in cropKheta Ram Tak
 
Antisense genes in plants and their applications in crop improvement
Antisense genes in plants and their applications in crop improvementAntisense genes in plants and their applications in crop improvement
Antisense genes in plants and their applications in crop improvementAmritaGiri5
 
gene silencing-for disease managment
gene silencing-for disease managmentgene silencing-for disease managment
gene silencing-for disease managmentBalaji Rathod
 
Gene Silencing (RNA interference )
Gene Silencing (RNA interference )Gene Silencing (RNA interference )
Gene Silencing (RNA interference )Mesele Tilahun
 

Mais procurados (20)

Principle and method of RNAi-Creative Biogene
Principle and method of RNAi-Creative BiogenePrinciple and method of RNAi-Creative Biogene
Principle and method of RNAi-Creative Biogene
 
RNA interference (RNAi):A therapeutic strategy for aquaculture
RNA interference (RNAi):A therapeutic strategy for aquacultureRNA interference (RNAi):A therapeutic strategy for aquaculture
RNA interference (RNAi):A therapeutic strategy for aquaculture
 
Rna interference in insect pest management
Rna interference in insect pest managementRna interference in insect pest management
Rna interference in insect pest management
 
Rna interference
Rna interferenceRna interference
Rna interference
 
Genetic engineering
Genetic engineeringGenetic engineering
Genetic engineering
 
Creative biogene-The Extended Applications of RNAi
Creative biogene-The Extended Applications of RNAiCreative biogene-The Extended Applications of RNAi
Creative biogene-The Extended Applications of RNAi
 
RNA interference in Biology and medicine
RNA interference in Biology and medicine RNA interference in Biology and medicine
RNA interference in Biology and medicine
 
Transgene silencing
Transgene silencingTransgene silencing
Transgene silencing
 
Rna interferences in crop improvement
Rna interferences in crop improvementRna interferences in crop improvement
Rna interferences in crop improvement
 
Rn ai @ sid
Rn ai @ sidRn ai @ sid
Rn ai @ sid
 
RNA interference
RNA interferenceRNA interference
RNA interference
 
RNA interference (RNAi)
RNA interference (RNAi)RNA interference (RNAi)
RNA interference (RNAi)
 
RNA INTERFERENCE TECHNOLOGY
RNA INTERFERENCE TECHNOLOGYRNA INTERFERENCE TECHNOLOGY
RNA INTERFERENCE TECHNOLOGY
 
Rna silencing
Rna silencingRna silencing
Rna silencing
 
RNA interference (RNAi) in plants
RNA interference (RNAi) in plantsRNA interference (RNAi) in plants
RNA interference (RNAi) in plants
 
Role of antisense RNAi in gene silencing in crop
Role of antisense RNAi in gene silencing in cropRole of antisense RNAi in gene silencing in crop
Role of antisense RNAi in gene silencing in crop
 
Antisense genes in plants and their applications in crop improvement
Antisense genes in plants and their applications in crop improvementAntisense genes in plants and their applications in crop improvement
Antisense genes in plants and their applications in crop improvement
 
Rna interference
Rna interferenceRna interference
Rna interference
 
gene silencing-for disease managment
gene silencing-for disease managmentgene silencing-for disease managment
gene silencing-for disease managment
 
Gene Silencing (RNA interference )
Gene Silencing (RNA interference )Gene Silencing (RNA interference )
Gene Silencing (RNA interference )
 

Destaque

2013programmed cell dealth r
2013programmed cell dealth r2013programmed cell dealth r
2013programmed cell dealth rtamoohy
 
Apoptosis
ApoptosisApoptosis
ApoptosisTKANHAR
 
Apoptosis in health and diseases
Apoptosis in health and diseasesApoptosis in health and diseases
Apoptosis in health and diseasesmeenuev
 
Chapt03 Holes Lecture Animation[1]
Chapt03 Holes Lecture Animation[1]Chapt03 Holes Lecture Animation[1]
Chapt03 Holes Lecture Animation[1]bholmes
 
Seminar- recent advances in apoptosis
Seminar- recent advances in apoptosisSeminar- recent advances in apoptosis
Seminar- recent advances in apoptosisEkta Jajodia
 

Destaque (11)

2013programmed cell dealth r
2013programmed cell dealth r2013programmed cell dealth r
2013programmed cell dealth r
 
Apoptosis
ApoptosisApoptosis
Apoptosis
 
Apoptosis
ApoptosisApoptosis
Apoptosis
 
Apoptosis in health and diseases
Apoptosis in health and diseasesApoptosis in health and diseases
Apoptosis in health and diseases
 
Apoptosis
ApoptosisApoptosis
Apoptosis
 
Chapt03 Holes Lecture Animation[1]
Chapt03 Holes Lecture Animation[1]Chapt03 Holes Lecture Animation[1]
Chapt03 Holes Lecture Animation[1]
 
Seminar- recent advances in apoptosis
Seminar- recent advances in apoptosisSeminar- recent advances in apoptosis
Seminar- recent advances in apoptosis
 
Apoptosis
ApoptosisApoptosis
Apoptosis
 
Apoptosis
ApoptosisApoptosis
Apoptosis
 
Apoptosis
ApoptosisApoptosis
Apoptosis
 
Apoptosis
Apoptosis Apoptosis
Apoptosis
 

Semelhante a Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

Genomics Technologies
Genomics TechnologiesGenomics Technologies
Genomics TechnologiesSean Davis
 
Encode jc 20130412
Encode jc 20130412Encode jc 20130412
Encode jc 20130412Dake Zhang
 
OKC Grand Rounds 2009
OKC Grand Rounds 2009OKC Grand Rounds 2009
OKC Grand Rounds 2009Sean Davis
 
Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...
Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...
Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...OECD Environment
 
Chemogenomic profiling
Chemogenomic profilingChemogenomic profiling
Chemogenomic profilingmeetpatel762
 
gene expression.pptx
gene expression.pptxgene expression.pptx
gene expression.pptxbreenaawan
 
Genome Sequencing in Finger Millet
Genome Sequencing in Finger MilletGenome Sequencing in Finger Millet
Genome Sequencing in Finger MilletVivek Suthediya
 
Molecular Markers: Major Applications in Insects
Molecular Markers: Major Applications in InsectsMolecular Markers: Major Applications in Insects
Molecular Markers: Major Applications in InsectsSaramita De Chakravarti
 
21 kebere bezaweletaw 207-217
21 kebere bezaweletaw 207-21721 kebere bezaweletaw 207-217
21 kebere bezaweletaw 207-217Alexander Decker
 
CrossGen-Merck manuscript
CrossGen-Merck manuscriptCrossGen-Merck manuscript
CrossGen-Merck manuscriptKush Sharma
 
Lab Report Green Fluorescent Protein
Lab Report Green Fluorescent ProteinLab Report Green Fluorescent Protein
Lab Report Green Fluorescent ProteinTina Jordan
 
Next Generation Sequencing and its Applications in Medical Research - Frances...
Next Generation Sequencing and its Applications in Medical Research - Frances...Next Generation Sequencing and its Applications in Medical Research - Frances...
Next Generation Sequencing and its Applications in Medical Research - Frances...Sri Ambati
 
E research feb2016 sifting the needles in the haystack
E research feb2016 sifting the needles in the haystackE research feb2016 sifting the needles in the haystack
E research feb2016 sifting the needles in the haystackTom Kelly
 
Chapter 20 ppt
Chapter 20 pptChapter 20 ppt
Chapter 20 pptrehman2009
 
Advanced genome & epigenome editing tools.pptx
Advanced genome & epigenome editing tools.pptx Advanced genome & epigenome editing tools.pptx
Advanced genome & epigenome editing tools.pptxberciyalgolda1
 
Recombinant Dna technology, Restriction Endonucleas and Vector
Recombinant Dna technology, Restriction Endonucleas and Vector Recombinant Dna technology, Restriction Endonucleas and Vector
Recombinant Dna technology, Restriction Endonucleas and Vector Dr. Priti D. Diwan
 
Transgenic animal models & their
Transgenic animal models & theirTransgenic animal models & their
Transgenic animal models & theirkalpanatiwari17
 

Semelhante a Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells (20)

Genomics Technologies
Genomics TechnologiesGenomics Technologies
Genomics Technologies
 
Encode jc 20130412
Encode jc 20130412Encode jc 20130412
Encode jc 20130412
 
OKC Grand Rounds 2009
OKC Grand Rounds 2009OKC Grand Rounds 2009
OKC Grand Rounds 2009
 
Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...
Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...
Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...
 
Chemogenomic profiling
Chemogenomic profilingChemogenomic profiling
Chemogenomic profiling
 
gene expression.pptx
gene expression.pptxgene expression.pptx
gene expression.pptx
 
Genome Sequencing in Finger Millet
Genome Sequencing in Finger MilletGenome Sequencing in Finger Millet
Genome Sequencing in Finger Millet
 
Molecular Markers: Major Applications in Insects
Molecular Markers: Major Applications in InsectsMolecular Markers: Major Applications in Insects
Molecular Markers: Major Applications in Insects
 
21 kebere bezaweletaw 207-217
21 kebere bezaweletaw 207-21721 kebere bezaweletaw 207-217
21 kebere bezaweletaw 207-217
 
CrossGen-Merck manuscript
CrossGen-Merck manuscriptCrossGen-Merck manuscript
CrossGen-Merck manuscript
 
Lab Report Green Fluorescent Protein
Lab Report Green Fluorescent ProteinLab Report Green Fluorescent Protein
Lab Report Green Fluorescent Protein
 
Next Generation Sequencing and its Applications in Medical Research - Frances...
Next Generation Sequencing and its Applications in Medical Research - Frances...Next Generation Sequencing and its Applications in Medical Research - Frances...
Next Generation Sequencing and its Applications in Medical Research - Frances...
 
E research feb2016 sifting the needles in the haystack
E research feb2016 sifting the needles in the haystackE research feb2016 sifting the needles in the haystack
E research feb2016 sifting the needles in the haystack
 
Chapter 20 ppt
Chapter 20 pptChapter 20 ppt
Chapter 20 ppt
 
Grindberg - PNAS
Grindberg - PNASGrindberg - PNAS
Grindberg - PNAS
 
Advanced genome & epigenome editing tools.pptx
Advanced genome & epigenome editing tools.pptx Advanced genome & epigenome editing tools.pptx
Advanced genome & epigenome editing tools.pptx
 
Recombinant Dna technology, Restriction Endonucleas and Vector
Recombinant Dna technology, Restriction Endonucleas and Vector Recombinant Dna technology, Restriction Endonucleas and Vector
Recombinant Dna technology, Restriction Endonucleas and Vector
 
MGG2003-cDNA-AFLP
MGG2003-cDNA-AFLPMGG2003-cDNA-AFLP
MGG2003-cDNA-AFLP
 
Molecular tagging
Molecular tagging Molecular tagging
Molecular tagging
 
Transgenic animal models & their
Transgenic animal models & theirTransgenic animal models & their
Transgenic animal models & their
 

Último

GenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day PresentationGenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day PresentationMichael W. Hawkins
 
08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
08448380779 Call Girls In Diplomatic Enclave Women Seeking Men08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
08448380779 Call Girls In Diplomatic Enclave Women Seeking MenDelhi Call girls
 
Data Cloud, More than a CDP by Matt Robison
Data Cloud, More than a CDP by Matt RobisonData Cloud, More than a CDP by Matt Robison
Data Cloud, More than a CDP by Matt RobisonAnna Loughnan Colquhoun
 
A Call to Action for Generative AI in 2024
A Call to Action for Generative AI in 2024A Call to Action for Generative AI in 2024
A Call to Action for Generative AI in 2024Results
 
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdfThe Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdfEnterprise Knowledge
 
Enhancing Worker Digital Experience: A Hands-on Workshop for Partners
Enhancing Worker Digital Experience: A Hands-on Workshop for PartnersEnhancing Worker Digital Experience: A Hands-on Workshop for Partners
Enhancing Worker Digital Experience: A Hands-on Workshop for PartnersThousandEyes
 
My Hashitalk Indonesia April 2024 Presentation
My Hashitalk Indonesia April 2024 PresentationMy Hashitalk Indonesia April 2024 Presentation
My Hashitalk Indonesia April 2024 PresentationRidwan Fadjar
 
Transcript: #StandardsGoals for 2024: What’s new for BISAC - Tech Forum 2024
Transcript: #StandardsGoals for 2024: What’s new for BISAC - Tech Forum 2024Transcript: #StandardsGoals for 2024: What’s new for BISAC - Tech Forum 2024
Transcript: #StandardsGoals for 2024: What’s new for BISAC - Tech Forum 2024BookNet Canada
 
08448380779 Call Girls In Friends Colony Women Seeking Men
08448380779 Call Girls In Friends Colony Women Seeking Men08448380779 Call Girls In Friends Colony Women Seeking Men
08448380779 Call Girls In Friends Colony Women Seeking MenDelhi Call girls
 
Understanding the Laravel MVC Architecture
Understanding the Laravel MVC ArchitectureUnderstanding the Laravel MVC Architecture
Understanding the Laravel MVC ArchitecturePixlogix Infotech
 
Scaling API-first – The story of a global engineering organization
Scaling API-first – The story of a global engineering organizationScaling API-first – The story of a global engineering organization
Scaling API-first – The story of a global engineering organizationRadu Cotescu
 
The Codex of Business Writing Software for Real-World Solutions 2.pptx
The Codex of Business Writing Software for Real-World Solutions 2.pptxThe Codex of Business Writing Software for Real-World Solutions 2.pptx
The Codex of Business Writing Software for Real-World Solutions 2.pptxMalak Abu Hammad
 
How to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected WorkerHow to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected WorkerThousandEyes
 
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...Igalia
 
Presentation on how to chat with PDF using ChatGPT code interpreter
Presentation on how to chat with PDF using ChatGPT code interpreterPresentation on how to chat with PDF using ChatGPT code interpreter
Presentation on how to chat with PDF using ChatGPT code interpreternaman860154
 
The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024Rafal Los
 
Swan(sea) Song – personal research during my six years at Swansea ... and bey...
Swan(sea) Song – personal research during my six years at Swansea ... and bey...Swan(sea) Song – personal research during my six years at Swansea ... and bey...
Swan(sea) Song – personal research during my six years at Swansea ... and bey...Alan Dix
 
Neo4j - How KGs are shaping the future of Generative AI at AWS Summit London ...
Neo4j - How KGs are shaping the future of Generative AI at AWS Summit London ...Neo4j - How KGs are shaping the future of Generative AI at AWS Summit London ...
Neo4j - How KGs are shaping the future of Generative AI at AWS Summit London ...Neo4j
 
04-2024-HHUG-Sales-and-Marketing-Alignment.pptx
04-2024-HHUG-Sales-and-Marketing-Alignment.pptx04-2024-HHUG-Sales-and-Marketing-Alignment.pptx
04-2024-HHUG-Sales-and-Marketing-Alignment.pptxHampshireHUG
 
FULL ENJOY 🔝 8264348440 🔝 Call Girls in Diplomatic Enclave | Delhi
FULL ENJOY 🔝 8264348440 🔝 Call Girls in Diplomatic Enclave | DelhiFULL ENJOY 🔝 8264348440 🔝 Call Girls in Diplomatic Enclave | Delhi
FULL ENJOY 🔝 8264348440 🔝 Call Girls in Diplomatic Enclave | Delhisoniya singh
 

Último (20)

GenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day PresentationGenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day Presentation
 
08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
08448380779 Call Girls In Diplomatic Enclave Women Seeking Men08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
 
Data Cloud, More than a CDP by Matt Robison
Data Cloud, More than a CDP by Matt RobisonData Cloud, More than a CDP by Matt Robison
Data Cloud, More than a CDP by Matt Robison
 
A Call to Action for Generative AI in 2024
A Call to Action for Generative AI in 2024A Call to Action for Generative AI in 2024
A Call to Action for Generative AI in 2024
 
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdfThe Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
 
Enhancing Worker Digital Experience: A Hands-on Workshop for Partners
Enhancing Worker Digital Experience: A Hands-on Workshop for PartnersEnhancing Worker Digital Experience: A Hands-on Workshop for Partners
Enhancing Worker Digital Experience: A Hands-on Workshop for Partners
 
My Hashitalk Indonesia April 2024 Presentation
My Hashitalk Indonesia April 2024 PresentationMy Hashitalk Indonesia April 2024 Presentation
My Hashitalk Indonesia April 2024 Presentation
 
Transcript: #StandardsGoals for 2024: What’s new for BISAC - Tech Forum 2024
Transcript: #StandardsGoals for 2024: What’s new for BISAC - Tech Forum 2024Transcript: #StandardsGoals for 2024: What’s new for BISAC - Tech Forum 2024
Transcript: #StandardsGoals for 2024: What’s new for BISAC - Tech Forum 2024
 
08448380779 Call Girls In Friends Colony Women Seeking Men
08448380779 Call Girls In Friends Colony Women Seeking Men08448380779 Call Girls In Friends Colony Women Seeking Men
08448380779 Call Girls In Friends Colony Women Seeking Men
 
Understanding the Laravel MVC Architecture
Understanding the Laravel MVC ArchitectureUnderstanding the Laravel MVC Architecture
Understanding the Laravel MVC Architecture
 
Scaling API-first – The story of a global engineering organization
Scaling API-first – The story of a global engineering organizationScaling API-first – The story of a global engineering organization
Scaling API-first – The story of a global engineering organization
 
The Codex of Business Writing Software for Real-World Solutions 2.pptx
The Codex of Business Writing Software for Real-World Solutions 2.pptxThe Codex of Business Writing Software for Real-World Solutions 2.pptx
The Codex of Business Writing Software for Real-World Solutions 2.pptx
 
How to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected WorkerHow to Troubleshoot Apps for the Modern Connected Worker
How to Troubleshoot Apps for the Modern Connected Worker
 
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
 
Presentation on how to chat with PDF using ChatGPT code interpreter
Presentation on how to chat with PDF using ChatGPT code interpreterPresentation on how to chat with PDF using ChatGPT code interpreter
Presentation on how to chat with PDF using ChatGPT code interpreter
 
The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024
 
Swan(sea) Song – personal research during my six years at Swansea ... and bey...
Swan(sea) Song – personal research during my six years at Swansea ... and bey...Swan(sea) Song – personal research during my six years at Swansea ... and bey...
Swan(sea) Song – personal research during my six years at Swansea ... and bey...
 
Neo4j - How KGs are shaping the future of Generative AI at AWS Summit London ...
Neo4j - How KGs are shaping the future of Generative AI at AWS Summit London ...Neo4j - How KGs are shaping the future of Generative AI at AWS Summit London ...
Neo4j - How KGs are shaping the future of Generative AI at AWS Summit London ...
 
04-2024-HHUG-Sales-and-Marketing-Alignment.pptx
04-2024-HHUG-Sales-and-Marketing-Alignment.pptx04-2024-HHUG-Sales-and-Marketing-Alignment.pptx
04-2024-HHUG-Sales-and-Marketing-Alignment.pptx
 
FULL ENJOY 🔝 8264348440 🔝 Call Girls in Diplomatic Enclave | Delhi
FULL ENJOY 🔝 8264348440 🔝 Call Girls in Diplomatic Enclave | DelhiFULL ENJOY 🔝 8264348440 🔝 Call Girls in Diplomatic Enclave | Delhi
FULL ENJOY 🔝 8264348440 🔝 Call Girls in Diplomatic Enclave | Delhi
 

Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

  • 1. Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells Michael Boutros, Amy A. Kiger, Susan Armknecht, Kim Kerr, Marc Hild, Britta Koch, Stefan A. Haas, Heidelberg Fly Array Consortium, Renato Paro, Norbert Perrimon (2004) Presenters: Lian Lim Luigi Leung
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14. Methods/Results - Observe cells (z-score >3)
  • 15.
  • 16.
  • 17. Method - Flow Cytometry
  • 18.
  • 19.
  • 20.
  • 21.
  • 22.
  • 23.
  • 24.
  • 25.
  • 26. Further Reading RNAi – A powerful tool to unravel hepatitis C virus–host interactions within the infectious life cycle Joachim Lupberger, Laurent Brino, Thomas F. Baumert Focusing on RISC assembly in mammalian cells Junmei Hong, Na Wei, Alistair Chalk, Jue Wang, Yutong Song, Fan Yi, Ren-Ping Qiao, Erik L.L. Sonnhammer, Claes Wahlestedt, Zicai Liang, Quan Du Analysis of Microarray Data Using Z Score Transformation Cheadle, C., Vawter, M., Freed, W., Becker, K.
  • 27.